1.In vitro Expansion of Natural Regulatory T Lymphocytes Useful for Cell Therapy in Allotransplantation.
Youn Hee PARK ; Sun Kyung KOO ; Yu Joung LEE ; In Yi JOE ; Song Cheol KIM ; Duck Jong HAN ; Dong Gyun LIM
Journal of Bacteriology and Virology 2009;39(3):217-227
Immunotherapy with regulatory T lymphocytes is considered to be an attractive new therapeutic modality to prevent allograft rejection. The success of this new therapy is critically dependent on the preparation of highly effective and enough number of regulatory T cells. Here, we tried to establish a proper strategy for the ex vivo expansion of regulatory T cells and evaluated their characteristics. CD4+CD25h+CD62L+ T cells were isolated from the recipient mice and weekly stimulated with various stimuli in the presence of IL-2. The most efficient protocol for the expansion of regulatory T cells maintaining Foxp3 expression and regulatory activity was the three cycles stimulation with donor bone marrow-derived dendritic cells (BM-DCs) which yielded around 400 fold expansion of regulatory T cells. The in vitro-expanded regulatory T cells expressing lymph node homing receptors on their cell surface, were composed of polyclonal population, and did not acquire the ability to produce effector cytokines. Importantly, these expanded regulatory T cells induced a modest prolongation of skin allograft survival when combined with transient T cell depletion in recipient mice. These data indicate that our protocol could be used to obtain an effective population of natural regulatory T cells available for the regulatory T cell therapy to prevent allograft rejection.
Animals
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Cytokines
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Dendritic Cells
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Humans
;
Immunotherapy
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Interleukin-2
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Mice
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Receptors, Lymphocyte Homing
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Rejection (Psychology)
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Skin
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T-Lymphocytes
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T-Lymphocytes, Regulatory
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Tissue Donors
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Tissue Therapy
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Transplantation, Homologous
2.Development of a contralateral acute subdural hematoma during awake craniotomy for glial tumor in a 12-year-old boy: A case report.
Han Bum JOE ; Sung Yong PARK ; Kwan Sik PARK ; Kyu Dong KYOUNG ; Yi Hwa CHOI ; Kyung Gi CHO ; Bong Ki MOON
Anesthesia and Pain Medicine 2011;6(2):157-159
Contralateral acute subdural hematomas that occur during removal of brain tumors under general anesthesia are extremely rare, and there are no reports of this developing during awake craniotomy for brain tumors. We report a case of a 12-year-old boy who complained of sudden and severe headache and nausea around the completion of removal of a glial tumor of the frontal lobe under awake anesthesia. Postoperative computerized tomography scan revealed the presence of contralateral acute minimal subdural hematoma. We suggest that during craniotomy with awake anesthesia for brain tumors, contralateral acute subdural hematoma may occur, even in the absence of brain bulging or changes in vital signs. Sudden intra-operative headache and nausea should be investigated by immediate postoperative computerized tomography scans to ascertain diagnosis.
Anesthesia
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Anesthesia, General
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Brain
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Brain Neoplasms
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Child
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Craniotomy
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Frontal Lobe
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Headache
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Hematoma, Subdural
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Hematoma, Subdural, Acute
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Humans
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Nausea
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Vital Signs
3.Development of a contralateral acute subdural hematoma during awake craniotomy for glial tumor in a 12-year-old boy: A case report.
Han Bum JOE ; Sung Yong PARK ; Kwan Sik PARK ; Kyu Dong KYOUNG ; Yi Hwa CHOI ; Kyung Gi CHO ; Bong Ki MOON
Anesthesia and Pain Medicine 2011;6(2):157-159
Contralateral acute subdural hematomas that occur during removal of brain tumors under general anesthesia are extremely rare, and there are no reports of this developing during awake craniotomy for brain tumors. We report a case of a 12-year-old boy who complained of sudden and severe headache and nausea around the completion of removal of a glial tumor of the frontal lobe under awake anesthesia. Postoperative computerized tomography scan revealed the presence of contralateral acute minimal subdural hematoma. We suggest that during craniotomy with awake anesthesia for brain tumors, contralateral acute subdural hematoma may occur, even in the absence of brain bulging or changes in vital signs. Sudden intra-operative headache and nausea should be investigated by immediate postoperative computerized tomography scans to ascertain diagnosis.
Anesthesia
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Anesthesia, General
;
Brain
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Brain Neoplasms
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Child
;
Craniotomy
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Frontal Lobe
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Headache
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Hematoma, Subdural
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Hematoma, Subdural, Acute
;
Humans
;
Nausea
;
Vital Signs
4.Efficacy of Itraconazole Melt-Extrusion Tablet One-week Therapy in Treatment of Hyperkeratotic Type of Tinea Pedis and/or Tinea Manus.
Kee Chan MOON ; Jai Kyoung KOH ; Baik Kee CHO ; Hyung Ok KIM ; Gun Su PARK ; Dae Gyu BYUN ; Jin Woo KIM ; In Kang JANG ; Jong Yuk YI ; Jae Bok JUN ; Tae Jin YOON ; Nack In KIM ; Kyu Suk LEE ; Chill Hwan OH ; Soo Nam KIM ; Sook Ja SON ; Yong Woo CHIN ; Dong Seok KIM ; Gwang Yeol JOE ; Won Woo LEE ; Kyung Sool KWON ; Dae Hun SUH ; Sang Eun MOON ; See Yong PARK ; Kea Jeung KIM ; Jong Suk LEE ; Eun So LEE ; Hyun Joo CHOI ; Eung Ho CHOI ; Ki Hong KIM ; Seung Hoon CHA ; Young Gull KIM ; Jung Hee HAHM ; Hae Young CHOI ; Sung Uk PARK ; Bang Soon KIM ; Sang Wahn KOO ; Byung Soo KIM ; Young Ho WON ; Han Uk KIM ; Eun Sup SONG ; Byoung Soo CHUNG ; Byung In RO ; Chang Kwun HONG ; Jagn Kue PARK ; Tae Young YOUN ; Hee Sung KIM ; Cheol Heon LEE ; Kwang Joong KIM ; Dae Won KOO ; Jong Min KIM ; Chang Woo LEE ; Hee Joon YU
Korean Journal of Dermatology 1999;37(8):1047-1056
BACKGROUND: Since the bioavailability of itraconazole capsule is influenced by patients gastric acidity, it results in treatment failure due to its low dissolution and subsequent low absorption when administered in fasting. Itraconazole Melt-Extrusion tablet has been lately developed in order to improve its dissolution profile. It is the first clinical study to evaluate the efficacy and safety of itraconazole Melt-Extrusion tablet in Korea. OBJECTIVE: This study was conducted to evaluate the efficacy and safety of itraconazole melt-extrusion tablet 400mg daily for 1 week(pulse therapy) for hyperkeratotic type of tinea pedis and manus. METHODS: A clinical and mycological investigation was made of 812 outpatients with hyperkeratotic type of tinea pedis and/or tinea manus who had visited at 52 general hospitals under the lead of the Korean Dermatological Association from June to December, 1998. Patients confirmed by clinically and microscopically as hyperkeratotic type of tinea pedis and/or tinea manus were administered 2 tablets twice a day for one week and followed up for 8 weeks from the start of the medication. RESULTS: The results were summarized as follows; 1. Clinical symptoms of hyperkeratotic type of tinea pedis and/or tinea mauns were significantly improved at the end of study, week 8(p<0.001). 2. Clinical response rate, defined as more than 50% decrease of the sum of the clinical symptom scores, was 79.3%(512/646). 3. Mycological cure rate, dafined as both culture and KOH negative at week 8, was 78.2%(244 /312). 4. 40(5.5%) patients, of the 727 patients evaluable for drug safety evaluation, were reported to have adverse event. CONCLUSION: Itraconazole Melt-Extrusion tablet 400mg/day for 1 week (pulse therapy) is effective and safe in the treatment of hyperkeratotic type of tinea pedis and/or tinea manus.
Absorption
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Biological Availability
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Fasting
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Gastric Acid
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Hospitals, General
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Humans
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Itraconazole*
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Korea
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Outpatients
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Tablets
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Tinea Pedis*
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Tinea*
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Treatment Failure