No abstract available.
Anus, Imperforate* ; Perineum* ; Ultrasonography*

It has been generally believed that the continuous flow of blood characterised by arterial inflow and venous outflow is essential for the survival of flaps. Since Baek et al.(1985) demonstrated the survival of a flap based on only a single vein without arterial inflow, there have been a lot of reports of successful skin flaps that depend on only venous blood flow. The location of pedicle is one of the important factors on the survival of flaps, but there have not been an effective study on the survival rates of venous island flaps according to their location of pedicles. In this study, we compared the survival rates of proximally based venous island flaps with those of distally based ones and attempted to decrease the difference, if any, in the survival rates between them. Distally or proximally based 4x3 cmvenous island flap, whose pedicle was a marginal branch of central vein, was elevated from the auricular cartilage of New Zealand white rabbit. IN the half of 20 rabbits, superoxide dismutase (SOD, 20,000 U/kg) was injected intravenously 30 minutes and 60 minutes after the flap elevation. On the 7th postoperative day, survival areas of the flaps were measured by image analysis system and the vascular network with the flaps were examined by micro fil cast. 40 flaps were divided as follows: proximally based venous flaps, distally based venous flaps, SOD treated proximally based venous flaps, SOD ttreated distally based venous flaps. The results were as follow: 1. The survival rate(79.39+/-9.24%) of the proximally based venous flaps were significantly higher than those(60.79+/-14.10%) of the distally based ones (p< 0.01). 2. SOD improved survival of the flap significantly (p< 0.05), and there was no statistical difference in the survival rates between proximally based venous flaps and SOD treated distally based venous flaps (p = 0.1785). 3. Revascularization was actively developed in the proximally based venous flaps and those flaps treated with SOD, but was minimal in the distally based venous flaps. These results suggested that the vascular pedicle would rather be located proximally than distally in designing the venous island flaps and that SOD treatment may be effective for the enhancement of survival of venous island flaps, especially in distally based ones.
Ear Cartilage ; New Zealand ; Rabbits ; Skin ; Superoxide Dismutase* ; Superoxides* ; Surgical Flaps ; Survival Rate ; Veins

No abstract available.
Constriction, Pathologic* ; Intestinal Atresia*

One of the most interesting subjects in the field of dermatology has become the clarifying of the action mechanism of various drugs in promoting skin permeability in regard to their usefulness or harmfulness to various skin diseases. Although dimethyl sulfoxide (DMSO) detergents and propylene glycol were well known as having a promoting effect on the skin permeability, but the exact action mechanism of them was still unknown because of difficulty in methodology. So an attempt to clarify the action mechanism and find out the differences in the mode of action between them have been made by using frog skin, which has been used in research for the 'membrane transport', during past but having complex structures i.e. soley as 'skin' in this study was done. Abdominal skin was removed from frogs, Rana nigromaculata, captured on the area of Kyung Ki Do. In a series of experimental procedures about effect of experimental agents, short circuit current (SCC) as an estimate of net flux of sodium transport and potential difference (PD) was measured by the method of Ussing and Zerahn(1951). And changes of SCC and PD was observed during treatment with bathing mediums which contained agents and maintained iso-osmorality by varying amount of sodium chloride. These were compared including the effect of concentration of each experimental agent. The experimental agents used were DMSO and various detergent, i.e., sodium lauryl sulphate(SLS): anionic detergent, benzalkonium chloride (BAC): cationic detergents, and 'Tween-80: nonionic detergents. Results obtained are summarized on the followings. 1. The promoting effect of the sodium lauryl sulphate on the active sodium transport was reached to maximum within 10 to 20 min and was present until 40 min. after bathing with it, and thereafter, sodium transport was inhibited irreversibly at the both sides of the frog skin. And the maximal promoting effect of the SLS induced by increasing concentration had relationship in such a way on the saturation curve. 2. The active sodium transport was always increased on the inside of the frog skin after bathing with benzalkonium chloride (BAC) and in spite of changing of bathing medium with Ringer's solution, the active sodium transport was steadiIy increased even beyond the effect of BAC except temporally decreasing just after the bathing with Riger's solution. But the frog skin died within 20 min. at the outside after bathing with BAC. 3. Tween-80 brought about reversible reaction increasing the active sodium transport across the frog skin at the inside after bathing. And it seemed to be parallel to the degree of tle coneentration of it. 4. The effect of dimethyl sulfoxide (DMSO) on the active sodium transport across the frog skin was altered seasonaly, i.e., promoting sodium transport during the summer months hut inhibiting it during the winter months. So it was suggested that the action of DMSO on the sodium transport depends on the metabolic rates of the frog skin and the action differed from that of ADH hormone which promotes the sodium transports only in winter months. Also the effect of the DMSO differed from various detergents in view of seasonal variation and reversibility of the action of it. From the above resu]ts, this experimental method should be used more preferably using isolated frog skin in the research for clarifying the action mechanisms of the various other drugs in skin permeability.
Baths ; Benzalkonium Compounds ; Dermatology ; Detergents ; Dimethyl Sulfoxide ; Permeability* ; Propylene Glycol ; Ranidae ; Seasons ; Skin Diseases ; Skin* ; Sodium ; Sodium Chloride

BACKGROUND: Several keratolytic agents have been used in many dirmatologic conditions such as callus, keratosis palmaris of plantaris, however the evaluation method of the effects of keratolytiic agents has not been good enough in clinical use. OBJECT: We have conducted an investigation to measure the effect of several keratolytic agents using an in vitro model. METHOD: We measured the fegraded protein of keratin by the bui iet method after adding enzymes such as trypsin, pepsin and papain, and irritants, salicylic acid and sodii m lauryl sulfate to the collected callus. RESULT: The order of the keratolytic effect of the enzymes was trypsir >pepsin>papain. It was difficult to detect the keratolytic effect of salicylic acid becaues of color hiidrance and there was an increasing tendency of keratolyti effect of sodium lauryl sulfate, however it was prominent mainly at a high concentration. CONCLUSION: These results suggested that the potency of similar types of keratolytic enzymes such as pepsin, trypsin and papain can be possible. However it was not such a sitable model to check the potency of the keratolytic effect of salicylic acid and the eoncentration tevel may be an important factor for certain kinds of chemicals such as sodium lauryl sulfate.
Bony Callus ; Irritants* ; Keratolytic Agents ; Keratosis ; Papain ; Pepsin A ; Salicylic Acid ; Sodium Dodecyl Sulfate ; Trypsin

PURPOSE: For the synthesis of surfactant protein(SP) peptides and production of next generation artificial pulmonary surfactant(PS), we have isolated SP-B, C from bovine PS, and studied the biochemical properties and amino acid sequences of these protein-peptides. METHODS: Crude surfactant and purified surfactant were isolated from materials extracted from the bovine lung lavage. The hydrophobic SP-B, C were purified by Sephadex LH 60 column chromatography from PS. The purities of SP-B, C were assessed by tricine buffer SDS-polyacrylamide gel electrophoresis and the amino acid sequences of these proteins were determined using Beckman PI-2090. RESULTS: The molecular weights of SP-B, C shown in SDS-polyacrylamide gel electrophoresis were as follows; 15,000-18,000(oligomer) Da for SP-B, 3,500-5,000 Da for SP-C. The amino acid sequences were; FPIPLPYCWL LRTLIKKIQA VIPKGVLAMT VAQCHVVPL LVGGIQQLV IEYSVILLTD TLLGRLPNLV CGLRLRCSG in SP-B, LIPCCPVNIK RLLIVVVVVV LLVVVIVGAL LMGL in SP-C, respectively. These results indicated that the amino acid sequences of bovine SPs were different. CONCLUSION: The SP-B, C were purified from bovine PS, and amino acid sequences of SP-B, C were determined. Further studies are needed for the development and use of next generations of exogenous PS preparation based on synthetic SP-peptides for the treatment of neonatal RDS in the future.
Amino Acid Sequence* ; Bronchoalveolar Lavage ; Chromatography ; Electrophoresis ; Family Characteristics ; Humans ; Infant, Newborn ; Molecular Weight ; Peptides ; Pulmonary Surfactants*

A 6(1/2)-year-old girl developed recurrent cholangitis following hepatic portoenterostomy for biliary atresia. Computed tomogram showed an ovoid cyst (4.5 x 4.0 cm in size) in the left hepatic lobe and another tubular dilatation (2.0 x 0.8 cm in size) in the right hepatic lobe. Percutaneous transhepatic cholangio-drainage (PTCD) with cystogram showed an ovoid cyst in the left hepatic lobe (Tsuchida type A), measuring 6.6 x 5.0 cmin size. She became afebrile and anicteric with aid of PTCD and parenteral antibiotics. However she continued to drain 45-150 cc of bile per day via the tube for over 2 weeks. Then she successfully underwent intrahepatic cystojejunostomy with guidance of intraoperative ultrasonography. This case illustrates relapsing cholangitis caused by Tsuchida type A intrahepatic cyst, which was successfully managed with PTCD followed by internal drainage procedure.
Anti-Bacterial Agents ; Bile ; Biliary Atresia* ; Cholangitis ; Dilatation ; Drainage ; Female ; Humans ; Portoenterostomy, Hepatic ; Ultrasonography

Background: Insulin is known to act as second effector that provides potassium transfer from extracellular fluid to intracellular fluid, thus causing hypokalemia and arrythmia. We evaluated the effect of magnesium sulfate treatment on hemodynamics and serum potassium concentration, induced by continuous infusion of regular insulin solution in rabbits. Method: Twenty-four rabbits were divided into four groups for the experiment. Group I was given intravenouse injection of 5 ml of normal saline and continuous dripping of 7.5 U of RI. Group II was pretreated with 30 mg/kg of magnesium sulfate, followed by continuous dripping of 7.5 U of RI. Group III was given 5 ml of normal saline and 15 U of RI. And Group IV was administered 15 U of RI after 30 mg/kg of magnesium sulfate pretreatment. Venouse blood sampling was done before pretreatment, and after 5, 30, 60 and 120 minutes respectively. Heart rate and arterial blood pressure were taken at the same intervals. Result: 1) There was no significant change in serum potassium concentration in groups injected with 7.5 units of RI respectively (groups I and II), regardless of magnesium pretreatment. But in groups injected with 15 units of RI, the group that was not pretreated with magnesium (Groups III) showed a decrease in serum potassium concentration from 3.39+/-0.57 Eq/L to 2.29+/-0.71 mEq/L, while the concentration in the pretreated group (Group IV) decreased more significantly from 3.35+/-0.50 mEq/L to 1.81+/-0.41 mEq/L. 2) Pulse rate did not change significantly in groups that received continuous infusion of 7.5 units of RI (groups I and II) regardless of magnesium pretreatment, but increased significantly in groups injected with 15 units of RI (groups III and IV), pretreated or not. The pretreated group (group IV) had a more significant rise in pulse rate compared with the group that was not pretreated (group III). 3) In all of the groups, those pretreated with magnesium (groups II and IV) and those not pretreated (groups I and III), there was no significant change in systolic and diastolic blood pressures. Nor was arrhythmia detected. Conclusion: The above results indicate that while magnesium sulfate pretreatment at 30 mg/kg with insulin infusion affects serum potassium concentration, has an antiarrhythmic effect, and may induce tachycardia, it does not have any significant effect on blood pressure.
Arrhythmias, Cardiac ; Arterial Pressure ; Blood Pressure ; Extracellular Fluid ; Heart Rate ; Hemodynamics* ; Hypokalemia ; Insulin ; Intracellular Fluid ; Magnesium Sulfate ; Magnesium* ; Potassium* ; Rabbits* ; Tachycardia

By performing the county-wide surveys of Paragonimus westermani infection in several areas in the Chilgok county, Kyungpook Province, three endemic foci of Paragonimus westermani were found. The foci found in this survey were Haksan first dong, Haksan second dong, and Settom in Kumhwa village, all in Kasan myun, Chilgok county. In the three communities, the positive rates for the fluke among the residents by the intradermal test with Paragonimus antigen varied from 22.3 per cent to 25.0 per cent; school children from these areas showed infection rates above 50 per cent. The rate in males and females was 23.9 and 23.0 per cent, respectively. This difference was not significant. The presence and number of Semisulcospira snails and Astacus crayfish, the intermediate hosts of Paragonimus westermani in the stream communities and the high infection rates for the fluke in the residents were the supporting factors in determining the foci.
parasitology-helminth-trematoda ; Paragonimus westermani ; paragonimiasis ; epidemiology ; host ; snail ; Semisulcospira snails ; Astacus crayfish

Clinical electrophysiological studies(EPS) were done in seven patients with chronic recurrent sustained ventricular tachycardia(VT) in an attempt to delineate the reproducibility and to select the effective antiarrhythmic drugs for the prevention of the recurrence of VT. We could induce and terminate the sustained VT, and could select the effective antiarrhythmic drugs in all patient in the cardiac catheterization laboratory with EPS. With these effective antiarrhythmic drugs VT did not recur for the follow up period of 15 to 20 months. In view of the serious nature of the VT and the demonstrated benefits of EPS, we could conclude that patients with chronic recurrent sustained VT should undergo EPS.
Anti-Arrhythmia Agents ; Cardiac Catheterization ; Cardiac Catheters ; Follow-Up Studies ; Humans ; Recurrence ; Tachycardia, Ventricular*