No abstract available.
Liver Diseases* ; Liver* ; Pregnancy*

OBJECTIVE: Recently, telomerase activity was found in normal physiologically regenerating cells as well as cancer cells or germ cells. Human endometrium is also physiologically regenerating tissues and undergoes regular and dynamic changes during the menstrual cycle. So the authors examined normal human endometrial tissues for telomerase activity according to menstrual cycle. METHOD: In the current study, 66 normal human endometrial tissues were analyzed for telomerase activity by a radioisotope polimerase chain reaction-based telomeric repeat amplification protocol assay. RESULTS: Of 27 proliferative phase endometrial samples, 22(81%) expressed telomerase activity, whereas 10 of 23(43%) secretory phase sample, 2 of 10(10%) postmenopausal sample, 1 of 6(17%) pregnant endometrial sample did(P<0.05). 4 of 27(15%) endometrial samples in the proliferative phase expressed high telomerase activity after 100-fold dilution of extract, whereas 1 of 23(4%) from secretory phase, none of the 10 postmenopausal and 6 pregnant endometrial samples did. The highest activity was observed in late proliferative phase. CONCLUSION: This study indicates that normal endometrium expresses telomerase, the activity of which changes dramatically and regularly during the course of the menstrual cycle.
Endometrium* ; Female ; Germ Cells ; Humans ; Menstrual Cycle* ; Telomerase*

Ever since the first intermediate host of Clonorchis sinensis was identified with Bulimus striatulus, it has been place to an important position in epidemics. One way to prevent Clonorchis sinensis is to exterminate Bulimus, which is itself the first intermediate host and there by to separate the life cycle of Clonorchis. In killing B. striatulus, nicotine sulfate and lindane have been chosen from insecticides which are widely used in farming areas. And then nicotine sulfate and lindane have been used to check their effectiveness in killing B. striatulus. In this experiment, the resistance of cercaria which parasites to B. striatulus has also been studied. Dipping method was used in the study. Nicotine sulfate and lindane have been used to check the mortality effect. Nicotine sulfate was used to check percentage of mortality in varied times. The existance of cercaria was tried only in nicotine sulfate. In the experiment of mortality effect of insecticides to B. striatulus and cercaria, the difference in killing rate and the resistance in different concentration and different length of time have been researched. It resulted as following: In the experiment with nicotine sulfate, the mortality increased with thicker concentration in the constant length of time. When compared the necessary liquid of nicotine sulfate and lindane in LD 50, nicotine sulfate was less used than lindane, but the mortality proved high. In the treatment to the same concentration of nicotine sulfate in different length of time, it proved that the longer period showed higher mortality. The mortality time required in LD 90 of nicotine sulfate was dependent on the concentration. And thicker concentration showed quicker effect. In the resistance of cercaria and B. striatulus to nicotine sulfate, the resistance of cercaria was proved to be stronger than that of B. striatulus.
parasitology-chemotherapy-helminth-trematode-Clonorchis sinensis ; Bulimus striatulus ; nicotine sulfate ; lindane

No abstract available.
Carbon Disulfide* ; Carbon* ; Korea*

Although there are many reports on the splenic (systemic) T cell response after Toxoplasma gondii infection, little information is available regarding the local T cell responses of peritoneal exudate cells (PEC) and gut intraepithelial lymphocytes (IEL) following peroral infection with bradyzoites. Mice were infected with 40 cysts of the 76K strain of T. gondii, and then sacrificed at days 0, 1, 4, 7 and 10 postinfection (PI). The cellular composition and T cell responses of PEC and IEL were analyzed. The total number of PEC and IEL per mouse increased after infection, but the ratio of increase was higher in IEL. Lymphocytes were the major component of both PEC and IEL. The relative percentages of PEC macrophages and neutrophils/eosinophils increased significantly at day 1 and 4 PI, whereas those of IEL did not change significantly. The percentage of PEC NK1.1 and gamma delta T cells peaked at day 4 PI (p < 0.0001), and CD4 and CD8 alpha T cells increased continuously after infection. The percentages of IEL CD8 alpha and gamma delta T cells decreased slightly at first, and then increased. CD4 and NK1.1 T cells of IEL did not change significantly after infection. IFN-gamma-producing PEC NK1.1 T cells increased significantly from day 1 PI, but the other T cell subsets produced IFN-gamma abundantly thereafter. The proportion of IEL IFN-gamma-producing CD8 alpha and gamma delta T cells increased significantly after infection, while IEL NK1.1 T cells had similar IFN-gamma production patterns. Taken together, CD4 T cells were the major phenotype and the important IFN-gamma-producing T cell subsets in PEC after oral infection with T. gondii, whereas CD8 alpha T cells had these roles in IEL. These results suggest that PEC and IEL comprise different cell differentials and T cell responses, and according to infection route these factors may contribute to the different cellular immune responses.
Acute Disease ; Animals ; Ascitic Fluid/cytology/*metabolism ; Female ; Interferon Type II/*biosynthesis ; Intestinal Mucosa/cytology ; Lymphocytes/*metabolism ; Mice ; Mice, Inbred C57BL ; Support, Non-U.S. Gov't ; T-Lymphocyte Subsets/*immunology ; Toxoplasmosis/*immunology

Many operative techniques for the repair of cleft lip nasal deformities have been reported. However, the situation with regard to nasal deformity is less satisfactorily understood than lip deformity, in which we have experienced significant improvement led by enhanced understanding of the total anatomic deformity of the primary cleft lip and the consequent refinement of surgical techniques. Our purposes in tip plasty are the restoration of nasal tip symmetry and correction of the obtuse angle of the cleft side nostril. Additionally, an inconspicuous scar is one of the merits of our method. The alar cartilage war exposed through both the alar rim and transcolumella incision, and then interalar loose connective tissue based superiorly was dissected and elevated. Cleft side alar cartilage was cross-hatched(Lipsett technique) on the deformed dome and relocated superomedially by a 4-0 vicryl fixation suture. The elevated intera1ar loose areolar tissue was used for augmentation of the alar dome. Conchal cartilage graft on cleft side dome and septoplasty were carried out in cases of specific necessity. We have performed this procedure in 19 patients, aged between 15 to 19. Average follow-up period was one year. Results have been rated good to excellent by patients and surgeons. There has been no recurrence of this deformity.
Cartilage ; Cicatrix ; Cleft Lip* ; Congenital Abnormalities* ; Connective Tissue ; Follow-Up Studies ; Humans ; Lip ; Polyglactin 910 ; Recurrence ; Sutures ; Transplants

It has been well known that the incidence of delayed or nonunion of tibial shaft fracture is high and in order to improve methods of treatment and to get satisfactory final results in these fractures, it is important to find out as early as possible whether the fracture is healing normally or not. The fracture healing is closely related to the circulatory conditions at the fracture site, however, in contrast to animal experiments it is impossible to visualize directly the circulatory conditions at the fracture site. There have been many efforts to visualize indirectly these vascular re-establishments through angiography. Kaski(1974) reported a paper concerning osteomedullography of tibial fracture with phlebocompression and noticed 5 types of veins related to the process of healing. We performed 54 cases of osteomedullography in 41 tibial fracture at 3 months after treatment and the following results were obtained: 1. The sinusoidal vein in the proximal fragment was observed most frequently and the next were intra-osseous crossing vein, periosteal callus vein, ascending branch of main efferent vein, and periosteal veins of proximal fragment in decreasig frequency. 2. The rate of positive finding in osteomedullography at 3 months after treatment was lower, and periosteal callus veins were observed more frequently in the group treated with only cast immobilization than in the group treated with plate and screws. 3. In the group treated with compression plate and screws, osteomedullographs were positive in all 3 cases within 3 months after operation and sinusoidal veins in the proximal fragment were visualized in all cases, however periosteal callus vein was not visualized at all. 4. After bone graft, the differentiation of periosteal callus from grafted bone was not easy in plain X-ray film, but osteomedullography was very useful in these cases. Positive findings were noticed in 2~3 months in all cases following bone graft. 5. The authors found that the ideal time of performing osteomedullography was 3 months following treatment. If found to be negative after 4 months following treatment, bone graft was necessary.
Angiography ; Animal Experimentation ; Bony Callus ; Fracture Healing ; Immobilization ; Incidence ; Tibial Fractures ; Transplants ; Veins ; X-Ray Film

PURPOSE: Chronic lung disease is a cause of significant morbidity and mortality in the neonatal respiratory distress syndrome. Although the exact mechanisms leading to the development of chronic lung disease are unknown, there is increasing evidence indicating that pulmonary inflammation contributes to the pathogenesis. The proinflammatory cytokines, interleukin(IL)-1beta, IL-8 may have a role to play in initiating the inflammatory response that is seen in chronic lung disease. In contrast, IL-10 is an antiinflammatory cytokine. The abnormal persistent inflammatory process in chronic lung disease may be due to dysregulation of these proinflammatory cytokines. So, to describe cytokine profiles of chronic lung disease in neonate, we analysed the IL-1beta, IL-8 and IL-10 levels in bronchoalveolar lavage fluid by ELISA technique. METHODS: We obtained bronchoalveolar lavage samples from 14 respiratory distress syndrome infants without chronic lung disease and 8 respiratory distress syndrome infants with chronic lungisease. The cytokine levels were assayed. RESULTS: The results were as follows: 1) IL-1beta levels were higher in chronic lung disease group(205.16+/-360.21pg/rnl) than group without chronic lung disease(21.21+/-26.14pg/ml)(P<0.05). 2) IL-8 levels were also higher in chronic lung disease group(166.16+/-347.26pg/ml) than group without chronic lung disease(49.85+/-105.27pg/ml)(P>0.05). 3) In contrast, IL-10 levels were lower in chronic lung disease group(78.29+/-138.69pg/ml) than group without chronic lung disease(111.29+/-155.27pg/ml)(P>0.05). CONCLUSION: These results suggest that the dysregulation of cytokines contributes to the pathogenesis of chronic lung disease. The difference in cytokine levels of bronchoalveolar lavage fluid can be used as a predictor for development of chronic lung disease.
Bronchoalveolar Lavage Fluid* ; Bronchoalveolar Lavage* ; Cytokines* ; Enzyme-Linked Immunosorbent Assay ; Humans ; Infant ; Infant, Newborn ; Interleukin-10 ; Interleukin-8 ; Lung Diseases* ; Lung* ; Mortality ; Pneumonia ; Respiratory Distress Syndrome, Newborn

No abstract available.

The carpal tunnel syndromes is one of the most common peripheral nerve entrapment syndromes. The typical symptoms are pain, numbness and paresthesia in the median nerve territory of the hand. Recently, it is widely recognized that occupational factor is regarded as the important cause of the carpal tunnel syndrome. Clinical study is performed in the 42 female workers who is repetitively working at packing department in a rayon manufacturing factory from November 1991 till March 1992. The study included a questionnaire, physical examinations, and the neurophysiological test. The summary of the results obtained was as follows: 1. Among 42 packing workers, 9 workers(21.4%) were diagnosed as carpal tunnel syndrome by electromyography. The affected side was bilateral in 4 workers(9.5%), right in 4 workers(9.5%), and left in one worker(2.4%). 2. Among 42 subjects, 28 workers(66.7%) complained the clinical symptoms related to carpal tunnel syndrome, 11 workers(26.2%) showed positive Phalen sign, and 7 workers(16.7%) showed positive Tinel sign. 3. Researchers regard electromyographic finding as the gold standard for diagnosis of carpal tunnel syndrome. The sensitivity and specificity of the clinical symptoms to diagnose the carpal tunnel syndrome were 0.89, 0.39 respectively. If the carpal tunnel syndrome is diagnosed by the combination of the positive findings of the symptoms and the physical examinations, either Tinel of Phalen sign, the sensitivity and specificity were 0.67 and 0.76 respectively. Considering above results, though this small number of worker is not adequate for epidemiologic conclusions, carpal tunnel syndrome seems to be an important occupational disorder among packing workers in a rayon manufacturing factory.
Carpal Tunnel Syndrome* ; Diagnosis ; Electromyography ; Female ; Fibrinogen ; Hand ; Humans ; Hypesthesia ; Median Nerve ; Occupations ; Paresthesia ; Peripheral Nerves ; Physical Examination ; Surveys and Questionnaires ; Sensitivity and Specificity