OBJECTIVE:To establish an UV method for the dermination of main components in rilmazafone hydrochloride tablets. METHODS: The content of rilmazafone was determined by UV spectrophotometry in accordance with Chinese Pharmacopeia (2005 edition). The detective wavelength was set at 254 nm. RESULTS: The linear range of rilmazafone was 2.5～30 ?g?mL-1 (r=0.999 9) and the average recovery was 99.41% (RSD=0.59%,n=10). CONCLUSION: The method is simple, accurate and applicable for the determination of rilmazafone in this preparations.

Objective:To explore the effects and problems of on-the-spot surveys to provide reference for promoting the investigation,analysis and evaluation of adverse drug reactions/events.Method:By the retrospective case study, 27 ADR/ADE death reports and their corresponding on-the-spot surveys were analyzed in Jiangsu province in 2008.Result: The death occurred more in the elderly,the ADR drags were more seen in the use of antibiotics,and ADRs occurred highly in the venous injection,and the the completion rate of the scene survey was 44.44%.Conclusion:The on-the-spot survey needs good cooperations from various departments,and the management of drugs should be strengthened.

Objective To study the effect of atorvastatin on the levels of high sensitive C-reactive protein (hs-CRP)in coronary heart disease(CHD)patients with different Gensini score.Methods 120 cases of CHD from March 201 1 to September 2015 in our hospital were selected as the research subjects.According to the different Gensini scores,they were divided into group A (Gensini score <40 ),group B (Gensini score 40 -60 ),group C (Gensini score 60 -80 ),group D (Gensini score >80 ),with 30 patients in each group.All patients were given atorvastatin treatment,the therapeutic effect of the four groups,the total cholesterol(TC),three glycerol(TG),high density lipoprotein cholesterol(HDL-C),low density lipoprotein cholesterol(LDL -C)and hs -CRP levels after treatment were compared.Results After treatment,the lower the Gensini score,the better curative effect,four groups had statistically significant difference in the therapeutic effects(P<0.05),and the total effective rates of group A,B, C and D were 96.7%,86.7%,76.6%,56.7%.After treatment,the TG,TC,HDL-C,LDL-C in four groups were improved,A group improved significantly better than the B group,B group improved better than those of C group,C group improved better than those of D group,the differences were statistically significant(F=6.253,6.258,5.856, 6.259,all P<0.05).After treatment,the levels of hs-CRP in group A,B,C and D were (8.2 ±3.9)mg/L,(11.9 ± 6.9)mg/L,(19.2 ±9.3)mg/L,(24.5 ±11.6)mg/L,the hs-CRP levels of four groups were lower,hs-CRP level after treatment in group A was lower than that of B,C,D groups;and hs-CRP level after treatment in group B was lower than that of group C,D;hs-CRP level of C group after treatment was lower than that of group D.The differ-ences were statistically significant(t=12.651,9.185,4.982,3.030,all P<0.05).Conclusion The level of serum hs-CRP in patients with CHD is higher,and the level of hs-CRP is positively correlated with the Gensini score.

OBJECTIVETo study the effect of nadroparin in the migration of breast cancer cells MDA-MB-231 and its action mechanism.

METHODThe MTT test was adopted to observe the effect of different concentrations of naringenin on the growth capacity of breast cancer cells MDA-MB-231. Wound healing and transwell experiment analysis were conducted to detect the effect of naringenin on the migration of breast cancer cells MDA-MB-231. Western blotting was adopted to investigate the effect of naringenin on protein expressions of MDA-MB-231 cell Integrin β3, β1 and matrix metalloproteinase MMP-2 and MMP-9. The computer virtual docking technique was used to evaluate the combining capacity of naringenin and Integrin β3 in vitro.

RESULTNaringenin inhibited the migration of MDA-MB-231 cells in a dose-dependent manner. In wound healing and transwell experiments, with the increase in the concentration of naringenin, the number of migrant MDA-MB-231 cells and the invasion capacity of breast cancer cells decreased. Naringenin could inhibit the protein expression of Integrin β3 in a dose-dependent manner, but with unobvious effect on expression of Integrin β1. Besides, naringenin could significantly inhibit the protein expressions of MMP-2 and MMP-9. The results of the computer virtual docking showed a negative value in the combining capacity between naringenin and Integrin β3, indicating the high affinity between them.

CONCLUSIONNaringenin can inhibit the growth capacity of breast cancer cells MDA-MB-231 and block the migration and invasion of breast cancer cells MDA-MB-231. Its mechanism is to down-regulate MMP-2 and MMP-9 expressions after combining with Integrin β3.

Breast Neoplasms ; drug therapy ; genetics ; metabolism ; pathology ; Cell Line, Tumor ; Cell Movement ; drug effects ; Drugs, Chinese Herbal ; pharmacology ; Female ; Flavanones ; pharmacology ; Gene Expression Regulation, Neoplastic ; drug effects ; Humans ; Integrins ; genetics ; metabolism ; Matrix Metalloproteinase 2 ; genetics ; metabolism ; Matrix Metalloproteinase 9 ; genetics ; metabolism ; Neoplasm Invasiveness

Objective To study the effect of dehydroepiandrosterone (DHEA) on the immune system by measuring the apoptosis and expression of Fas/FasL mRNA of thymocytes. Methods The cell viability of thymocytes was determined by MTT assay. Thymocyte apoptosis was detected by agrese gel electrophoresis. The expression of Fas and Fas-L was analyzed by a semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) method. Results DHEA decreased the cell viability of thymocytes and induced thymocytes apoptosis and increased the levels of mRNA of Fas/FasL. Conclusion DHEA can induce apoptosis of thymocytes through Fas/Fas-L pathway. It suggests that DHEA may play a role in certain autoimmune diseases related to apoptosis disturbance.

Applications of molecular diagnostics in clinical microbiology laboratory were introduced in the article,including nucleic acid hybridization,nucleic acid amplification,DNA sequencing,gene chips and mass spectrometry.Molecular diagnostic techniques provide major tools for rapid diagnosis of infectious diseases,molecular epidemiology investigation,rapid identification of microbial pathogens and the study of the pathogenicity and antibiotics resistance.The application of these new technologies,as the complement of traditional culture methods,increases sensitivity,accuracy and diagnostic efficiency of assays.

Pim-3,a new member of the the proto-oncogene pim family which expresses serine and threonine kinase activity,shows a high similar to pim-1 and pim-2 at the function,structure and sequence.Pim-3 plays an important regulative role in the cell cycle transform,cell apoptosis and occurrence of the malignant tumors by phosphorylating numerous specific substrates.

Objective To discuss the association between vitamin D level and the severity of diabetic retinopathy and maculopathy in type 2 diabetes mellitus.Methods 657 patients with type 2 diabetic patients were included in our study and classified into four groups according to their severity of diabetic retinopathy:the non-diabetic retinopathy group(NDR group, n=257) ,the background diabetic retinopathy group(BDR group, n=243), the preproliferative diabetic retinopathy group(PPDR group, n=135) and the proliferative diabetic retinopathy group(PDR group, n=22).The patients were divided into maculopathy(n=563) and non-maculopathy(n=94).Characteristics data were recorded and disease duration, body mass index, blood pressure and other lipid makers were measured and compared among groups.Results Among four diabetic retinopathy groups, 25(OH)D was statistically different (P <0.05), however, compared to diabetic maculopathy group, the level of 25(OH)D in non-maculopathy group was not statistically different.Logistic regression showed that 25(OH)D was significantly associated with the severity ofdiabetic retinopathy(P<0.05), while not with diabetic maculopathy.Conclusion The level of 25 ( OH ) D is closely associated with the severity ofdiabetic retinopathy in patients with type 2 diabetes mellitus, and it might be an important predictor for the severity of diabetic retinopathy.

The Wnt/β-catenin signal transduction pathway is essential for regulating cell proliferation, movement, differentiation and embryo development. Researchers have found that Wnt pathway is an important regulation factor in the early stimulation of the cardiogenesis and contacts closely the several key events during cardiac morphogenesis. The article reviews the recent studies about the relation between cardiac development and Wnt pathway in early embryo.

Aim To investigate the effects of curcumin on expression of HIF-1? in human hepatocellular carcinoma BEL-7402 cells.Methods Different concentrations of 0,2.5,5,10,15 and 20 ?mol?L~(-1) curcumin-treated human hepatocellular carcinoma BEL-7402 cells were cultured under hypoxia for 6 hours.The cell proliferation and cell viability were assayed by WST-8 and the trypan blue dye exclusion method.The expression level of HIF-1? in human hepatocellular carcinoma BEL-7402 cells was checked by RT-PCR and Western Blot;0,10 ?mol?L~(-1) curcumin,10 ?mol?L~(-1) MG-132,and 10 ?mol?L~(-1) curcumin +10 ?mol? L~(-1) MG-132-treated human hepatocellular carcinoma BEL-7402 cells were cultured under hypoxia for 6 hours.The protein expression level of HIF-1? protein in human hepatocellular carcinoma BEL-7402 cells was checked by Western Blot.Results ① No significant difference was found in the cell proliferation rate and cell viability between control and different concentrations of curcumin.② Curcumin can down-regulate the expression of HIF-1? protein with the increasing concentrations.③ the effect of curcumin on expression of HIF-1?mRNA had no significant difference between control and different concentrations.④ MG-132 can reverse the curcumin-mediated decrease of HIF-1? protein.Conclusion Inhibitory effect of curcumin on expression of HIF-1? in human hepatocellular carcinoma BEL-7402 cells was acted through posttranscriptional mechanisms and the proteasome pathway.