Acupuncture Points ; Acupuncture Therapy ; Adolescent ; Adult ; Female ; Humans ; Hysteria ; therapy ; Male ; Middle Aged ; Paralysis ; therapy ; Young Adult

Heart Septal Defects, Ventricular ; genetics ; pathology ; Humans

The silver doped poly ( L-lysine ) modified glassy carbon electrode was fabricated by cyclic voltammetry, the surface of the electrode was characterized by scanning electron microscopy and electrochemical impedance spectroscopy. The electrochemical behaviors and the simultaneous detection of xanthine and uric acid were studied by cyclic voltammetry and differential pulse voltammetry. The results indicated that this modified electrode exhibited excellent electrocatalytic activity towards the oxidation of xanthine and uric acid. The stable oxidation peaks of xanthine and uric acid appeared with the peak potential of 0. 980V and 0. 600V respectively at the modified electrode in pH 3. 0 phosphate buffer solution. The oxidation peaks of xanthine and uric acid were separated at 380 mV. Under the optimum conditions, the linear ranges for the determination of xanthine and uric acid were 1 . 00 × 10-6-2 . 50 × 10-4 mol/L respectively by differential pulse voltammetry. The detection limits were 5. 0×10-7mol/L. The method has been applied to the simultaneous detection of xanthine and uric acid in healthy human urine with satisfactory results.

Objective To observe the value of detection of pulmonary arterial hypertension(PAH) with curvature ratio measured at cardiac MR.Methods We performed a retrospective study of 48 patients with cardiac MR,systemic systolic blood pressure and right heart catheterization(RHC) data.The parameter curvature ratio (Rc) was measured at cardiac MR and transseptal/transmural pressure ratio (RP) was calculated.By using the regression analysis between Rc and RP,the SPAP was calculated.Bland-Altman analysis was used to compare the SPAP calculated with SPAP obtained by RHC.The accuracy of the SPAP calculated in detecting PAH was analyzed by using receiver operating characteristic (ROC) curves (SPAP higher than 40 mmHg as cutoff).The normality of parameter is tested by K-S test.Bivariate correlation Pearson test was used to test the consistency of parameters obtained by different measurers.Linear regression analysis was performed to find the relationship between Rc and RP.Results Parameter RP =0.28 ± 0.37 (-0.50-0.83) and RC =0.31 ± 0.57 (-0.78-0.97).Linear relationship was found between Rc and RP (r =0.94,P ＜ 0.01) and regression equation:Rc =1.44 × RP-0.09.Bland-Altman analysis showed satisfactory limits of agreement with the SPAP calculated and obtained by RHC.ROC analysis of the diagnostic value in PAH revealed 94.4％ (34/36) of sensitivity and 100.0％ of specificity (12/12,area under ROC curve =0.942,P ＜ 0.01).Conclusions Our study suggests that with curvature ratio measured at cardiac MR,the SPAP estimated have considerable sensitivity and specificity for detecting PAH.It may be a useful non-invasive assessment in patients that suspected of PAH.

AIM: Agricultural and industrial wastes, such as deteriorated corns and oil foots, were used as slow-release materials to prepare biodegradable puffed fertilizers, with urea was encapsulated in fertilizer particles with three-dimensional (3D) skeleton construction.METHODS: Slow-release materials formed 3D skeleton construction through modified treatments, swelling, comminution,extrusion and pelleting. Also 3D skeleton construction and its scales were modified by alternating technical conditions,species and ratio of materials in order to realize the controlled-release of fertilizers.RESULTS: Puffed slow-release fertilizers were prepared. And 3D skeleton construction formed as a compact protective layer on surface of fertilizer particles, whose coating fraction of urea was larger than 85%, the number of pin hole (larger than 20 nm) was lower than 20%, and thickness of membrane was larger than 30 nm. The protective layers of samples in soil were biodegraded and pulled out by microbes. Therefore fertilizer particles emerged gradually and dissolved into soil. Slow release of puffed fertilizers was a continuous process containing degradation, abscission and dissolution. And the whole procedure did not yield any polluting residuals. Release experiments in water showed that pure urea (100%) dissolved absolutely in 10 minutes, whereas only 8.2% urea was released from samples with urea content 85.50% after 120 minutes.And similar experiments in soil illuminated that 85% urea was dissolved from pure urea (100%) in 20 hours, whereas 1.3% and 0.8% urea was released from puffed slow-release fertilizers with urea content 81.5% and 40.9% respectively. Therefore,the release rate of slow-release puffed fertilizers was only 1/65.38 and 1/106.25 to that of pure urea.CONCLUSION: Compared with pure urea, the release rates of puffed slow-release fertilizers in both water and soil are apparently low. Furthermore, puffed slow-release fertilizers have good biodegradability (＞95%), which can improve the construction of soil and increase the content of organic fertilizers.

Objective To evaluate the efficacy of deanxit in treatment of post-stroke depression (PSD).Methods60 patients with PSD were randomly divided into treatment group ( plus with deanxit) and control group (plus with estazolam necessarily). At the baseline and at 2 weeks,4 weeks, 8 weeks after the treatment, the efficacy was assessed with HAMD, SSS, and ADL rating scale respectively. Results After 2,4,8 weeks treatment, HAMD and SSS scores of the treatment group significantly decreased compared with the control group ( all P ＜ 0. 01 ), and ADL scores of the treatment group increased significantly compared with the control group ( P ＜ 0. 01 ). Conclusion Deanxit could effectively relieve depressing symptoms and neurological impairment of PSD, and clinical side effects of deanxit were less. Deanxit was suitable for acute phase and long-term maintenance therapy in PSD.

Objective To establish the optimum preparation process for Yinxingye Dropping Pills. Methods The preparation conditions, such as different kinds of primary substance, cooling agent, the proportion between the extracts and primary substance, etc, were studied with L9 (34) orthogonal design. Results At the following conditions could the best was 30 d/min, dropping distance was 8cm, and cooling temperature was 10℃. Conclusion This preparation process was suitable for both laboratory test and industrial production for Yinxingye Dropping Pills.

Objective To explore the test-retest reliability of elevated plus maze (EPM) which is taken as an animal model of state anxiety in Kunming mice.Methods The behavior of adult,male and female,Kunming mice in EPM was recorded twice with one-week inter-trial interval for lasting five minutes.The following parameters were evaluated:percentage of time on EPM open arms (Otime％),percentage of time on EPM closed arms (Ctime％),time on EPM open arms (Otime),time on EPM closed arms (Ctime),percentage of entries into EPM open arms (Oentries％),entries into EPM open arms (Oentries),entries into EPM closed arms (Centries),total entries into EPM open and closed arms (Entries).Subsequently,the intraclass correlation coefficient (ICC) and the kappa agreement index (κ) were calculated for these parameters.Results ICC for Otime％ (♂ ICC=0.506,P＜0.05;♀ ICC=0.456,P＜0.05;♂ + ♀ ICC=0.492,P ＜0.01),Oentries (♂ ICC=0.673,P＜0.01;♀ ICC=0.648,P＜0.01;♂ + ♀ ICC=0.665,P＜0.01),Centries (♂ ICC=0.704,P＜0.01;♀ ICC=0.661,P＜0.01;♂ + ♀ ICC=0.686,P＜0.01),Entries (5 ICC=0.701,P＜0.01;♀ ICC=0.661,P＜0.01;5 + ♀ ICC=0.685,P＜0.01) showed a fair test-retest reliability,respectively.Only κ for Entries (♂ κ=0.253,P＜0.05;♀ κ=0.339,P＜0.05;♂ + ♀ κ=0.298,P＜0.01)displayed a significant but poor reliability between the test-retest trials.Conclusion The Otime％ Entries parameters as an acceptable index for Kunming mice in EPM can be used for exploratory and locomotion activity.Sex and repeated measurement have different effects on the Otime％ and Entries parameters,especially the more on the former.Therefore,Otime％ and Entries can be considered when EPM as an animal model of state anxiety in Kunming mice;meanwhile,more attention should be paid to both the sex (male animals recommended to EPM,especially) and repeated measurement effects.

Hypoxic pulmonary pressor response (HPPR) was observed witb a preparation of perfused lungs in situ in rats, and the effects of deoxy-2-glucose (2DG), verapamil, and dipyridamole on HPPR were studied.During bypoxia (inhalation of 3% oxygen for 10 minutes) , there was a 50% increase on average in pulmonary arterial pressure in the perfused lungs in situ in rats. 2DG inhibited the first and second but enhanced the seventh and eighth HPPR. Both verapamil and dipyridamole depressed HPPR.On the basis of the results, the author suggests that the mechanism of HPPR might originate from the lungs themselves. There might be a close relation between the glucose-energy metabolism and the occurrence of HPPR, the trans-membranous influx of calcium might play a certain role in the development of HPPR, and adenosine would probably participate in the regulation of HPPR.

Objective:To examine the expression of integrin ?V?3 in periodontium during experimental tooth movement, and then to propose the possible role of ?V?3 in bone remodeling during tooth movement. Methods:Thirty-five rabbits were divided into 7 groups (5 rabbits per group):untreated(control), 1,3, 5, 7, 14 and 21 d groups. A elastic coil spring was ligated between the incisors and the first molar and exert a force of approximately 0.78 N to pull the first molar mesially. The animals were sacrificed at each time intervals(1,3,5,7,14 and 21 d) respectively and specimens were made to observe the expression of integrin ?V?3 in periodontium through in situ hybridization. Results:Different extent expression of integrin ?V?3 were seen in osteoclasts, osteoblast precursors, osteoblasts,odontoclasts and odontoblast except bone cells. Conclusions: Integrin ?V?3 may play an important role in bone modeling, cementum resorption and its reparation during experimental tooth movement.