After the synthesis of IL-15 cDNA from the total RNA of mouse spleen, it was inserted into the prokaryotic expression vector, pRseta, and eukaryotic expression vector, pcDNA3.0, respectively. Subsequently, the insertion of gene and open reading frame were confirmed by sequencing of each plasmid, respectively. Using pRseta- IL-15 plasmid, the recombinant IL-15 protein was induced by IPTG under BL21 (DE 3) host cells and recombinant IL-15 was expressed at 14.5 KDa with time. Then, IL- 15 was separated by His-tag affinity chromatography and analyzed by SDS-PAGE to yield soluble IL-15 at 14.5 KDa as monomer and 29.0 KDa as dimer. In order to inspect the function and contribution of IL-15, the in vitro experiment was established using mononuclear cells separated from the mouse spleen. After 48h exposure of PHA to mouse splenocyte and 24h treatment with recombinant IL-15, the effects of cytokine inductions inspected against IL-2, IL-6, IL-10, IL-12, IFN-r, and GM-CSF. The results showed that comparing with the control, IL-6 increased, IL-2, IL-12 and IFN-r increased and similar, and GM-CSF decreased. In addition, the direct injection of pcDNA3.0-IL-15 plasmid into mice gave the similar results to in vitro studies. Namely, IL-6 and IL-12 increased, and IL-2, IFN-r and GM-CSF were similar or decreased. IL-10 was not induced in in vitro and in vivo experiments. These results suggested that the IL-15 induce the splenocyte activation and can be an important factor in proliferation and fuction recovery of weakened T-cell.
Animals ; Chromatography, Affinity ; DNA, Complementary ; Electrophoresis, Polyacrylamide Gel ; Granulocyte-Macrophage Colony-Stimulating Factor ; Interleukin-10 ; Interleukin-12 ; Interleukin-15* ; Interleukin-2 ; Interleukin-6 ; Isopropyl Thiogalactoside ; Mice* ; Open Reading Frames ; Plasmids ; RNA ; Spleen ; T-Lymphocytes

To determine the causes of and related factors to childhood injuries, the emergency room records and inpatient medical records were reviewed for 4,849 injured children out of 15,790 pediatric patients(<15 years old) who visited the emergency rooms of 3 university hospitals and 2 general hospitals in Taegu from 1 January to 31 December 1987. Out of total injured children, 54.6% were 3-8 years old and the male to female ratio of the total injured children was about 2 : 1. The leading causes of injury were falls and slips (29.1%) and traffic accident(28.2%). The frequency of injury was higher in May-October than the rest of months and 51.6% of the injuries occurred between 15 and 20 o'clock. Falls and slips took place most frequently at the stairway(25.7%). The most common interpersonal violence was inflicted injuries(85.6%) and there were 11 child rapes. Dog bites accounted for 67.6% of all biting injuries and it occurred 2.9 times more in male than in female. CO intoxication was the most common cause of poisoning (45.3%) and scalding accounted for 85.2% of all burns. Common places of drownings were river (32.2%), swimming pool (22.6%) and construction site(19.3%). To prevent childhood injuries, it is recommended to eliminate the hazardous environmental factors, to provide safe playgrounds, to educate the children for safety from kindergarten and the general public through mass communication, to establish a strict safety standard for houses, public buildings and facilities, and playgrounds.
Animals ; Burns ; Child ; Daegu* ; Dogs ; Drowning ; Emergencies* ; Emergency Service, Hospital* ; Female ; Hospitals, General ; Hospitals, University ; Humans ; Inpatients ; Male ; Medical Records ; Poisoning ; Rape ; Rivers ; Swimming Pools ; Violence

No abstract available.
Intercellular Adhesion Molecule-1* ; Prurigo*

Cyclic strabismus is now well recognized, but remains a rare entity. This heterotropia represents an interesting ocular motility problem in which the ocular deviation is present on a rhythmic basis, usually a 48 hour cycle. On the strabismic day, constant heterotropia is large and associated with suppression and no diplopia. On the nonstrabismic day, no deviation or only a small heterophoria is present with good binocular function. Most of these strabismus were presented with esotropia, but in our case, the pattern of deviation is cyclic exotropia. This is a very rare and unique feature.
Diplopia ; Esotropia ; Exotropia* ; Strabismus ; Telescopes

No abstract available.
Animals ; Rats*

PURPOSE: This study aimed at uncovering the disabled adult's way of thinking and behavior due to their changed life style. METHOD: In depth interviews with 11 participants were held and analysed based on the Grounded Theory Methodology by Strauss and Corbin (1990). RESULT: This study showed us that the disabled are getting independent by selfconfidence through developing coping strategies for their physical, emotional, social, sexual problems. And in the process of creating these strategies, support structures, satisfaction for performance effect, financial burdens, and mobility had an effect on the intervening factors. Adults who become disabled go through many changes. First, they have vague hopes of improving. Second, they become discouraged. Third, they accept their disability. Next, they learn coping strategies and finally, they integrate the changes into their new life style. CONCLUSION: This study can be utilized in rehabilitation nursing so that the disabled can adapt to their changed life style quickly and lead independent and self-confident lives.
Adult* ; Hope ; Humans ; Life Style* ; Rehabilitation Nursing ; Thinking

PURPOSE: to test of the reliability and validity of the Disabled's Motivation for Rehabilitation Scale. which was developed in 2002. METHOD: An experimental version of the scale was distributed to a sample of 441 disabled with ages above 18 and below 80 years. The subjects of the test-retest were 60 disabled. RESULTS: revealed a satisfactory level of test-retest and internal consistency. The overall fit of the factor model to the data was good. Correlation among the subscales revealed a simple pattern that, in general. provides support for the self- determination continuum and the construct validity. In testing concurrent, criterion-validity, there was a positive correlation between the motivation scores for rehabilitation and the Health-Related Hardiness scores and a negative correlation between the motivation scores for rehabilitation and the Learned Helplessness scores. CONCLUSION: The Disabled's Motivation for Rehabilitation Scale revealed a useful instrument with a high degree of reliability and validity. In this sense, this instrument can be effectively utilized in rehabilitative nursing for the disabled.
Helplessness, Learned ; Motivation* ; Nursing ; Rehabilitation* ; Reproducibility of Results*

PURPOSE: This study was conducted to evaluate successful aging (SA) in elderly individuals and to determine the factors that influence SA. METHODS: The subjects included 207 elderly individuals from D city, Korea. The data were obtained between July 1 and August 30, 2008. The participants were assured of anonymity and confidentiality. For the evaluation, Kim & Shin's SA scale, Lawton's instrumental activities of daily living (IADL) scale, Jon's self-esteem scale, Kee's geriatric depression scale short form-Korean (GDSSF-K), and Hong's productive activities scale were used. The data were analyzed using descriptive statistics, the t-test, analysis of variance (ANOVA), and Pearson correlation coefficients; multiple regression analysis was performed using the SPSS/WIN 10.0 program. RESULTS: The maximum score for SA was 34, and the mean score was 22.62. It was found that SA was significantly correlated to IADL, self-esteem, depression, and productive activity. Depression was the most powerful predictor of SA and it accounted for 40.6% of the total variance in SA. Self-esteem, productive activity, living situation, economic status, and age together accounted for 62.1% of the total variation in SA. CONCLUSION: Depression, self-esteem, and productive activity were identified as variables that influence SA. On the basis of these results, we conclude that nurses should assist elderly individuals in order to facilitate SA. Moreover, individualized nursing management strategies must be developed in order to facilitate SA.
Activities of Daily Living ; Aged ; Aging ; Anonyms and Pseudonyms ; Confidentiality ; Depression ; Humans ; Korea

The purpose of this research of this research was to survey the health of 181 male and 130 female students at K University in Chunganm from June 1 to June 23, 2001. It analyzed the students' dietary behaviors, life styles and perceived body shapes and the correlation of these factors to the health condition of the students surveyed. The results of the survey are summarized as follows. The average nutrition knowledge score (out of a possible 20 points), dietary attitudes score (out of a possible 50 points) and dietary habits score (out of a possible 30 points) were 8.66, 33.14 and 19.02 for males, and 9.99, 32.64, and 19.05 for females, respectively. The percentages of those who smoked, drank, smoked and drank and exercised regularly were 55.2, 92.8, 54.1, 82.9% for males, and 6.9, 91.5, 6.9, 63.1 for females, respectively. A significantly higher proportion of males smoked, drank, and exercised as compared to females (p<0.001). The percentages of students who were underweight, normal or overweight by BMI were 5.0, 79.0 and 16.0 for males, and 20.0, 78.5 and 1.5 for females, respectively. Fifty percent of females indicated that they felt they were obese, versus only 31.1% of males. Half of the females thought they were fattish, while only 31.1% of males thought that. Seventy-six point nine percent of females and 61.9% of males, more females than males, were dissatisfied with their weights. The health scores (the higher the point, the better the health condition) of the females (44.17) were significantly lower than those of the males (47.17) (p<0.001). A stepwise multiple regression analysis with health scores as the dependent variable and other scores as independent variables, indicated that the health scores of males were highly correlated with satisfaction with his body weight, smoking and drinking, and perceived body shape, whereas the females' health scores were highly correlated with obesity level, satisfaction with her body weight, and smoking and drinking.
Body Weight ; Chungcheongnam-do* ; Drinking ; Female ; Food Habits* ; Humans ; Life Style* ; Male ; Obesity ; Overweight ; Smoke ; Smoking ; Thinness ; Weights and Measures

We performed immunohistochemical stain of p-glycoprotein using JSB-1 monoclonal antibody to study multidrug resistant(MDR) gene expression in 137 gastric tumor tissues obtained from 87gastric carcinoma patients. The incidence of p-glycoprotein expression was 60 of 87 cases(69%) and it was not correlated with age, sex, depth of tumor invasion, and lymph node metastasis, but was correlated with histologic type of gastric adenocarcinoma. The distribution of p-glycoprotein positive cells in the tumor tissue was diffuse in 34 cases(73.9%) and focal in 12 cases(26.1%), and the dominant staining patterns of p-glycoprotein in the tumor cells were cytoplasmic and golgi staining in 20 cases(43.5%) and 19 cases(41.3%), respectively, and 7 cases(15.2%) showed fine granules in the cytoplasm. The incidence of p-glyco-protein expression in the tumor tissue was higher in A and AB blood type patients who have A antigen than in 0 and B blood type patients. Cytoplasmic staining pattern was dominant in O and B blood types and golgi staining in A and AB blood type patients. Among 27 patients 'who received chemotherapy, partial remission was noted in 9 of 11 p-glyco-protein negative patients(81.8%) and no remission or progression of the tumor was seen in 9 of 16 p-glycoprotein positive patient(56.3%). The p-glycoprotein expression in gastric carcinoma had no direct correlation with known several prognostic factors of the gastric tumor except for histologic type, and it is supposed that p-glycoprotein detection in gastric tumor tissue by immunohisto-chemical stain is a good method for predicting the response of chemotherapy, especially in p-gly-coprotein negative cases.
Incidence