OBJECTIVES: It has been identified a novel protein in porcine and bovine follicular fluid termed Gonadotropin Surge Inhibiting Factor (GnSIF) which inhibits GnRH-stimulated LH secretion by pituitary cells in culture. The purpose of this study was to investigate the production of GnSIF by porcine granulosa cell in vitro. MATERIALS AND METHODS: Granulosa cells were aspirated from different size of porcine follicles (1-3mm, 3-5mm, >6mm in diameter) and cultured in 24-well plates in Ham's F-12/DMEM at 500,000 cells/well for 24h. After plating, cells were washed and incubated in triplicate for 72h in DMEM/F-12 containing 2% FBS in the presence of 100ng/ml FSH and the media were collected. Granulosa cell-conditioned media were chromatographed on heparin-Sepharose to remove inhibin and follistatin, and assayed for GnSIF activity using a rat pituitary cell bioassay. Briefly, dispersed pituitary cells from adult female Sprague-Dawley rats were plated (500,000 cells/well) for 72h, washed, and incubated with granulosa cell-conditioned media in the presence of 10nM GnRH for 4h. The media were collected and assayed for LH by RIA. GnSIF activity is defined as the suppression of GnRH-stimulated LH secretion. RESULTS: Granulosa cell-conditioned media significantly inhibited GnRH-stimulated LH secretion by rat pituitary cells in vitro (69% of control, P<0.001, n=2 experiments). Preliminary data suggest that granulosa cells from small follicles (1-3mm) produce greater amounts of GnSIF than those obtained from larger follicles (>6mm). CONCLUSIONS: GnSIF activity is produced by porcine granulosa cells in vitro. Granulosa cell production of GnSIF, along with other gonadal proteins such as inhibin, follistatin, and activin may be an important component of the ovarian control of pituitary gonadotropin secretion.
Activins ; Adult ; Animals ; Biological Assay ; Female ; Follicular Fluid ; Follistatin ; Gonadotropin-Releasing Hormone ; Gonadotropins* ; Gonads ; Granulosa Cells* ; Humans ; Inhibins ; Rats ; Rats, Sprague-Dawley

The present study attempted to induce an acquired immunity against the dog hookworm, Ancylostoma caninum. The experiments were carried out to demonstrate and confirm whether an acquired resistance could be developed by the repeated lowgrade infections with the infective larvae of A. caninum in the abnormal host of mouse. In order to determined the distribution of hookworm larvae in the lungs and liver of mice after inoculation of infective larvae, 54 mice were inoculated with 1,000 larvae and sacrificed in batches daily up to 9 days after infection. It was found that in all cases the average total number of larvae recovered from the lungs and liver increased at 48 hours after infection, then began to decrease and reached 0 to 1 at the 9th day after inoculation. One hundred fifteen mice were immunized 2 or 4 times at 7 day intervals with 50 infective larvae, followed by challenging infection with 1,000 infective larvae and killed in batches at 48 hours after challenge. The interval between challenge and last immunization were from one to four weeks for each group. Sixty mice as the controls were given only challenging infection without previous immunization infection. Induced resistance was evaluated by the rate of recovery or the average total number of the larvae recovered from the lungs and liver in the challenged mice, compared with the controls. It was noted that the rates of recovery in the controls were twice or nearly higher than those in the previously infected mice in all instances and these remained low for the first 7 day intervals between the last immunizing infection and challenge, then gradually increased. From the above observations it is highly suggested that an acquired resistance can be produced by repeated previous infections with the larvae of A. caninum, even in low grade, in the abnormal host of mouse, and evaluated by the rate of recovery of the larvae after challenge.
parasitology-helminth-nematode-Ancylostoma caninum ; immunology ; recovery rate

This study was made to investigate the effect of adenoidectomy on dentofacial skeleton in naso-respiratory dysfunction children. The clinical material compromised the 24 children in a previous study who had naso-respiratory dysfunction and 24 children who were the nasal breathing with normal occlusion. The cephalograms were taken at the initial examination and 1 year later for the control group and experimental group the paired sample statistical analysis was performed. The results were as follows. 1. In cranial base variable, difference between two groups were not statistically significant. 2. In craniofacial variables, experimental group showed brachyfacial pattern but control groups didn't show significant growth pattern. 3. In maxillary variables, experimental group showed flattening the plane. 4. In mandibular variables, experimental group showed the decrease of mandibular plane angle and gonial angle. 5. In facial height variables, experimental group showed horizontal growth rotation.
Adenoidectomy* ; Adenoids ; Child* ; Humans ; Respiration ; Skeleton* ; Skull Base

Radioactive C(14)-glycine was given to Clonorchis sinensis in Tyrode medium in order to trace the metabolic fate of the labeled carbon. The labeled carbon from glycine enters into every major fraction of Clonorchis sinensis and is highest in the fraction of protein and nucleic acid. Significant amount of C(14)-glycine is incorporated into respiratory carbon dioxide. Relatively high percentage of C(14)-glycine in medium is converted to amino acid fraction and lipid fraction of the worm. In general, glycine is continuously being utilized in the synthesis of proteins and for energy production despite the uptake rate of glycine decreased gradually as incubation proceeds.
parasitology-helminth-trematoda ; Clonorchis sinensis ; metabolism ; biochemistry ; glycine ; amino acid ; nucleic acid ; protein ; lipid ; Tyrode medium

No abstract available.
Thyroidectomy*

The purpose of this study was to examine the relationship among variables which are categorized by two groups : the first group consists of obesity, serum lipids, and blood pressure which were measured by physical index or physiological index, and the second group consists of demographic variables and variables related to lifestyle that is known to influence the first group. The canonical correlation analysis was conducted with the data collected from 400 male clients who visited one university hospital located in Inchon in a period, from May 1996 to December 1996, for physical examination. According to the results, blood pressure and HDL of the first group were shown to have strong relationship with age, alcohol, smoking, exercise of the second group. And total cholesterol, triglyceride, systolic, BMI of the first group were shown to have strong relationship with income status, education, exercise of the second group. And all of the serum lipids (total cholesterol, triglyceride, HDL), BMI, and diastolic were shown to have relationship with education, smoking, alcohol. It should be noted that fat rate, which was one of the indices for obesity, was not significant in any of the canonical variates. From the comparative study results on which combination of indices of obesity, serum lipids and blood pressure was related to either alcohol or smoking more, it can be seem that smoking was more highly related to two blood pressure indices such as systolic and diastolic, and that alcohol was highly related to serum lipid indices such as HDL, total cholesterol, triglyceride. Also investigation on what differences exist between the combination of variables showing high relationship to systolic and the combination of variables showing high relationship to diastolic was conducted. Systolic turned out to have relationship with total cholesterol, triglyceride, obesity (fat rate), income status, education, exercise. And diastolic was related to HDL-c, total cholesterol, tryglyceride, obesity (BMI), education, smoking, alcohol. From this results, it could be seen that the combination of variables which have high relationship to systolic and diastolic have different patterns. Lastly, the investigation on what relationships exist between serum lipids and demographic variables/lifestyle variables was conducted. It turned out that HDL-c had high relationship with age, education, income status, alcohol, and that all of serum lipids (total cholesterol, triglyceride, HDL) were shown to have high relationship with income status, education, age, exercise. From these results, it could be seen that the demographic variables were more influential both on HDL and on all of serum lipids than lifestyle variables were.
Arteriosclerosis* ; Blood Pressure ; Cholesterol ; Education ; Humans ; Incheon ; Life Style ; Male ; Obesity ; Physical Examination ; Risk Factors* ; Smoke ; Smoking ; Triglycerides

No abstract available.
Hemophilia A* ; Humans

Most fractures of the proximal humerus are managed well to simple conservative treatment. But open reduction and interhal fixation of the fragment is definitely indicated in certain types of displaced fractures. Thirty eight cases of fractures of the proximal humerus which were treated at Inje Medical College from January 1980 to December 1985 were analyzed both clinically and radiologically accordingto Neer's classification and the following results were obtained. l. Among the 38 patients, the ratio of male and female was 2.2:1 and the highest incidence was in the fifth decades. 2. The main cause of fracture was traffic accident, 20 cases (52. 6%) and the the next one was falling from a height. 3. According to the classification of Neer, one part acture(52.6%) was the most common type. 4. The common associated injuries were fracture of the lower extremities and forearms. 5. Thirty two cases(84.2%) were treated by conservative treatment and six cases by operative treatment. 6. Thirty eight cases were followed form 6 months to 5 years and 6 months, average 14.3 months, and the results were analyzed according to Neers criteria and 26 cases (68. 6%) showed excellent results. 7. Two cases of limitation of shoulder motion were observed as a complication and one cases of paralysis of deltoid muscle by axillary nerve injury was observed. Excellent functional result could be obtained by a simple external support with early motion in a minimal displacement of proximal humeral fracture. Satisfactory functional result could be obtained by a open reduction and internal fixation with early motion in a severely displaced fracture. But long term immobilization of shoulder could have a poor functional result.
Accidental Falls ; Accidents, Traffic ; Classification ; Clinical Study ; Deltoid Muscle ; Female ; Forearm ; Humans ; Humerus ; Immobilization ; Incidence ; Lower Extremity ; Male ; Paralysis ; Shoulder ; Shoulder Fractures

With the expending use of sublaminar segmental instrumentation, various complication, including paraplegia, paresis, dysesthesia and dural tears, have been reported. These findings suggest that the displacement of sublaminar wires toward the spinal eord during extraction followed by spinal cord damage. The cut surface of Luque sublaminar wire is sharp and has a barb and it may cause damage to the spinal cord. The cut end of Luque wire was inserted into the Intracath (a kind of angiocatheter) 16 gauge and pulled parallel to the lamina. And we found the Intracath followed by Lugue wire in the operating field. Lateral view of spine roentgenogram revealed that the radioopaque Intracath was passed under the lamina in 3 mm in depth. When the Luque wire is removed with inserting into the Intracath 16 gauge and pulled parallel to the lamina, the dura can be protected by the cut end of Luque wire.
Paraplegia ; Paresis ; Paresthesia ; Spinal Cord ; Spine ; Tears

Large cell neuroendocrine carcinoma (LCNEC) of the lung is a newly recognized entity of pulmonary neuroendocrine carcinoma. Histologically, it is very difficult to differentiate LCNEC from other pulmonary carcinomas and the prognosis is significantly poor. The cutaneous metastasis of LCNEC of the lung shares some features with Merkel cell carcinoma of the skin in light microscopy and yet it is negatively stained with cytokeratin 20. We report a case of cutaneous metastasis of LCNEC of the lung, previously misdiagnosed as squamous cell carcinoma. Our patient showed a poor response to the chemotherapy and also revealed a brain metastasis on follow-up brain CT scan.
Brain ; Carcinoma, Merkel Cell* ; Carcinoma, Neuroendocrine* ; Carcinoma, Squamous Cell ; Drug Therapy ; Follow-Up Studies ; Humans ; Keratin-20 ; Lung* ; Microscopy ; Neoplasm Metastasis ; Prognosis ; Skin ; Tomography, X-Ray Computed