An experimental study of the tensile bonding strength of acrylic bone cement was carried out by using the tubular bones of 12 mongolian dogs. Following observations were made: 1. The later the cementing stage, the weaker was the tensile bonding strength of bone cement. 2. When saline or synovial fluid was interposed between the contact surfaces of the recipient bone and the cement, the tensile bonding strength of bone cement decreased by more than 50% of that when the bonding surface was dry. 3. When the bonded bone was stored in Hartmans solution, the tensile bonding strength of bone cement was reduced to 15–25% of the strength when stored in air.
Animals ; Dogs ; Polymethyl Methacrylate ; Synovial Fluid

One of the primary functions for which bones have evolved is to act as a structural support. To achieve this, bones remodel throughout life so that their structure remains optimal for the prevailing mechanical environment. Bone remodeling consists of an initial phase of osteoclastic bone resorption followed by a bone formation period. Prostaglandins are potent regulators of bone formation and bone resorption that can have both stimulatory and inhibitory effects. Elevation of intracellular cAMP is an important intracellular signaling mechanism involved in the regulation of the expression of many proteins. In this study we examine whether PGE or DBcAMP affects osteoblastic activation or osteoclastic differentiation in mouse bone marrow cells and osteosarcoma ROS 17/2.8 cells. The effect of PGE and DBcAMP on the cell proliferation was measured by the incorporation of [3H]- thymidine into DNA. As a result, PGE2 (0.5-1 ug/ml) and DBcAMP (0.1-0.5 mM) inhibited the [3H]-thymidine incorporation into DNA in a dose dependent manner. The effect of PGE2 and DBcAMP on the induction of alkaline phosphatase (ALP) was investigated in ROS 17/2.8 cells cultured in medium containing 0.4% fetal bovine serum. PGE and DBcAMP stimulated ALP activity in the cells in a dose- dependent manner. PGE2 also increased the intracellular cAMP content in a dose- dependent fashion with a maximal effect at 0.5 ug/ml. ROS 17/2.8 cells release nitric oxide upon stimulation of PGE2 or DBcAMP with interferon-r. PGE2 and DBcAMP increase the phosphorylation level of CREB (cAMP response element binding protein) without any change on the amount of CREB protein. Also, PGE (10-6 M) and DBcAMP (10-4 M) significantly increase the generation of osteoclasts in mouse bone marrow cell culture system. In conclusion, the results of this study suggested that cAMP appears to be an important regulatory molecule in the processes of bone formation and resorption.
Alkaline Phosphatase ; Animals ; Bone Marrow Cells ; Bone Remodeling ; Bone Resorption ; Bucladesine* ; Cell Proliferation ; Cyclic AMP Response Element-Binding Protein ; Dinoprostone* ; DNA ; Metabolism* ; Mice ; Nitric Oxide ; Osteoblasts ; Osteoclasts ; Osteogenesis ; Osteosarcoma ; Phosphorylation ; Prostaglandins ; Prostaglandins E ; Response Elements ; Thymidine

As a preliminary step to determine if we can perform methacholine challenge test in young children, We investigated the feasibility of modified technique of methacholine challenge test in which tidal breathing and chest auscultation were used instead of dosimeter and measurement of lung function in older children. The results are as follows: 1) Values of PC20 measured by the tidal breathing method correlated significantly with those of PC20 measured by dosimeter 2) Ratio of values (PC20) obtained wth both methods was not related to age. 3) The minimal concentration of methacholine which caused wheezing on chest ausculation (PCW) correlated closely with PC20, but was 44% greater on average. 4) Expiratory wheezing is associated with greater fall of FEVI from baseline than inspiratory wheezing. Thus wheezing on chest auscultation can be used to detect bronchial obstruction at the end point for bronchial provocation testing in older children during the quiet tidal inhalation of methacholine. This may be applied to measurement of airway reactivity in young children who are unable to perform routine pulmonary function tests.
Auscultation* ; Bronchial Provocation Tests ; Child ; Humans ; Inhalation ; Lung ; Methacholine Chloride ; Respiration* ; Respiratory Function Tests ; Respiratory Sounds ; Thorax*

No abstract available.
Doxorubicin* ; Membrane Potentials* ; Membranes* ; Purkinje Fibers* ; Sodium*

For comparison of clinical classification of leprosy to histopathologic classification, a detailed histopathologic study, using hematoxylin and eosin stain and Ziehl-Neelsen stain, was done on 72 fresh uncomplicated cases of leprosy. The clinical classification was done using the criteria of Ridley and Jopling (1966), and the microscopic features were classified according to Ridley's(1974) definition. Clinical classification revealed that 8 of total 72 patients had tuberculoid(TT), 9 had borderline tuberculoid (BT), 5 had borderline(BB), 10 had borderline lepromatous.(BL), and 31 had lepromatous leprosy(LL). Nine patients were claasified as indeterminate(I) group. Histopathologic classification showed that 3 cases presented tuberculoid(TT), 10 presented borderline tuberculoid(BT), 4 presented borderline(BB), 9 presented borderline lepromatous(BL), 20 presented subpolar leprornatous(LLs), and 10 presented polar lepromatous(LLp) histopathologic characteristics, Sixteen cases were classified as indeterminate(I) leprosy by histopathologic findings. On comparison of clinical classification to histopathologic classification, the two were in consonance with each other in 50 cases(69.4%) and the disparity between them was noticed in 22 cases(30.6%). Among the 22 cases which showed disparity, there was a shift of one step either towards the tuberculoid or lepromatous end of the spectrum in 15 cases, and ihe remaining 7 cases were classified as indeterminate group beaause of nonspecific histopathologic changes.
Classification* ; Eosine Yellowish-(YS) ; Hematoxylin ; Humans ; Leprosy* ; Leprosy, Paucibacillary

No abstract available.
Muscular Atrophy*

BACKGROUND: This study was aimed at defining the varying responses of porcine coronary artery(PCA) to various wavelengths of ultraviolet irradiation, and at relating them to the changes in cyclic GMP contents. METHODS: The ring preparations of PCA with intact or removed endothelium were irradiated with the ultraviolet or visible light of wavelengths(240-520mm) from xenon lamp of a spectrofluorometer, and the changes in vascular tension were recorder on polygraph. For cyclic GMP assay, rat thoracic aorta was frozen after irradiation and homogenated. The supernatant was extracted with water-saturated ether and the cyclic GMP contents were measured with radioimmunoassay. RESULTS: Ultraviolet irradiation relaxed the preparations(UVR-relaxation) in resting state and those precontracted by prostaglandin F2alpha, the maximal relaxation occurring at 410nm, and the magnitude depending on the duration of irradiation. The UVR-relaxation was not affected by removing the endothelium, while it was markedly potentiated by pretreatment with Bay K 8644. The Bay K 8644-induced potentiation of UVR-relaxation was abolished by hemoglobin and slightly reduced by wrapping the rings with aluminum foil. Cyclic GMP contents in the increase was markedly potentiated by pretreatment with Bay K 8644. CONCLUSION: The observations suggest that UVR-relaxation in procine coronary artery is caused by activating the nitric oxide-cyclic GMP system, which is most sensitively activated by UVR of 410nm and that its potentiation induced by Bay K 8644 may be related nitrous substance released from the agent upon UVR.
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester* ; Aluminum ; Animals ; Aorta, Thoracic ; Bays* ; Coronary Vessels* ; Cyclic GMP ; Dinoprost ; Endothelium ; Ether ; Light* ; Passive Cutaneous Anaphylaxis ; Radioimmunoassay ; Rats ; Relaxation* ; Ultraviolet Rays ; Vasodilation ; Xenon

BACKGROUND: Wheezing which is defined as a continuous sound with a musical quality is commonly auscultated in patients with chronic obstructive airway diseases. The correlation between wheezing and airway obstruction is unclear. OBJECTIVE: This study was designed to evaluate the relationships among wheezing, severity of airway obstruction, and pulmonary function tests. METHOD: Forty-one subjects were examined by the same observer. Wheezing during normal breathing and maximal forced exhalation, was auscultated respectively. Posterior lung bases were auscultated bilaterally with the seated patient taking repeated inspiratory capacity breaths through an open mouth. To quantify wheezing intensity, a regional score was assigned for each area after a minimum of 3 breaths, according to the following scale: zero, no wheezing heard: one, faint or intermittent wheezes: two, moderate wheezing during every expiration: three, loud wheezing during every expiration. The lung function tests by standard pneumotachograph were performed by skilled technicians. RESULTS: Wheezing was auscultated more in forced exhalation than in normal breathing in patients with asthma and COPD [8/9(88%) vs 1/9(11%), p<0.01 ll/15(73%) vs 1/15(6%), p<0.05)]. Forced expiratory wheezes group (n=25) compared to no wheezes group (n=16) had significantly lower FEVl (75+-5.8% vs 95.6+-6.6%, p<0.05). Compared to no wheezes group, the group with forced expiratory wheezes had lower FEV1 and FEV1/FVC (50.4+- 21.3% vs 81.15+-27.7%, 70.4+-22.4% vs 92.5+-19.3%, respectively, p<0.05). Bronchial asthma compared with COPD tended to have higher wheezing scores (Wheeze scores Bronchial asthma 3.5 vs COPD 2.4, p=0.08). Wheezing scores were correlated to FEV1 (normal breathing: r=-0.35, p<0.05: forced exhalation: r=-0.45, p<0.05), but no differences were found in wheezing incidence according to severity of airway obstruction. CONCLUSION: These findings suggest that wheezing on maximal forced exhalation may be a useful physical indicator for evaluating the severity of airway obstruction.
Airway Obstruction ; Asthma ; Exhalation ; Humans ; Incidence ; Inspiratory Capacity ; Lung ; Mouth ; Music ; Pulmonary Disease, Chronic Obstructive* ; Respiration ; Respiratory Function Tests ; Respiratory Sounds*

No abstract available.
Endometriosis* ; Female ; Leiomyoma* ; Ovary*

Toxic epidermal necrolysis(T.E.N.) is a toxic erythema of the skin that undergoes epidermal necrolysis and peding so that it appears to have been scalded. There seem to be two basic form of T.E.N. In children, the deavage of the epidermis that results from an acantholytic-dyskeratotic cytolytic process is in the malpighian or granular layer and is usually induced by a staphylococci toxin. In adult, it is characterized by a full-thicknees cytolytic cleavage of skin above the basal layer and is usually precipitated by a drug. The authors observed a 17 year-old male with T.E.N. He began to notify the erythematous skin lesion with burning sensation on the whole body surface at 12 hours after oral administration of some drugs for common cold and developed the characteristic flaccid bullous and denuded lesions with tenderness within next 20 hours.
Administration, Oral ; Adolescent ; Adult ; Burns ; Child ; Common Cold ; Epidermis ; Erythema ; Humans ; Male ; Sensation ; Skin