1.Easy Application of Digoxigenin-11-dUTP Labelled Probe in Detection of Human Papillomavirus DNA.
Annals of Dermatology 1991;3(2):91-95
In situ hybridization was performed in ten cases of condyloma acuminata in order to study the applicability of digoxigenin-11 dUTP(Dig-dUTP) labelled probe compared with radioactive isotope labelled probes. Although signal intensity was denser in radiolabelled probes, high positive rates were obtained with Dig-dUTP labelled probes. From these results, Dig-dUTP labelling is found to be more efficient in typing of human papillomavirus DNA than radiolabelling.
DNA*
;
Humans*
;
In Situ Hybridization
2.Easy Application of Digoxigenin-11-dUTP Labelled Probe in Detection of Human Papillomavirus DNA.
Annals of Dermatology 1991;3(2):91-95
In situ hybridization was performed in ten cases of condyloma acuminata in order to study the applicability of digoxigenin-11 dUTP(Dig-dUTP) labelled probe compared with radioactive isotope labelled probes. Although signal intensity was denser in radiolabelled probes, high positive rates were obtained with Dig-dUTP labelled probes. From these results, Dig-dUTP labelling is found to be more efficient in typing of human papillomavirus DNA than radiolabelling.
DNA*
;
Humans*
;
In Situ Hybridization
3.Detection of herpesviruses in renal allograft rejection by in situ hybridization.
Wan Shik SHIN ; In Sik PARK ; Yoon Sik CHANG ; Yung Jin CHOI ; Sang In SHIM ; Jin Han KANG ; Jin Hong YOO ; Yang Lee KIM ; Yung Suk YOON ; Moon Won KANG ; Byung Kee BANG
Korean Journal of Infectious Diseases 1993;25(4):351-355
No abstract available.
Allografts*
;
Herpesviridae*
;
In Situ Hybridization*
4.Characterizing Atypical BCL6 Signal Patterns Detected by Digital Fluorescence In Situ Hybridization (FISH) Analysis.
Michael LIEW ; Leslie R ROWE ; Phillipe SZANKASI ; Christian N PAXTON ; Todd KELLEY ; Reha M TOYDEMIR ; Mohamed E SALAMA
Annals of Laboratory Medicine 2018;38(6):619-622
No abstract available.
Fluorescence*
;
In Situ Hybridization*
6.Molecular biomarkers detected using fluorescence in situ hybridizationin a Filipino with retinoblastoma
Arnold Dominic A. Barzaga ; Glenmarie Angelica S. Perias ; Lia Angela E. Reyes ; Patrick Gabriel G. Moreno ; Patrick R. Relacion ; Richelle Ann M. Manalo ; Yasmyne C. Ronquillo ; Francisco M. Heralde III
Acta Medica Philippina 2024;58(10):99-107
Background and Objective:
Retinoblastoma is one of the most common intraocular cancers among children usually caused by the loss of retinoblastoma protein function. Despite being a highly heritable disease, conventional diagnostic and prognostic methods depend on clinical examination, with limited consideration of cancer genetics in the standard of care. CD133, KRT19, and MUC1 are commonly explored genes for their utility in liquid biopsies of cancer including lung adenocarcinoma. To date, there are few extensive molecular studies on retinoblastoma in Filipino patients. To this end, the study aimed to describe the copy number of CD133, KRT19, and MUC1 in retinoblastoma samples from a Filipino patient and quantitate the respective expression level of these genes.
Methods:
Hematoxylin & Eosin (H&E) staining was utilized to characterize the retinoblastoma tissue while fluorescence in situ hybridization (FISH) using probes specific to CD133, KRT19, and MUC1 was performed to determine the copy number of genes in retinoblastoma samples from a Filipino patient (n = 1). The gene expression of CD133, MUC1, and KRT19 was quantitated using RT-qPCR.
Results:
The H&E staining in the retinoblastoma tissue shows poorly differentiated cells with prominent basophilic nuclei. CD133 was approximately 1.5-fold overexpressed in the retinoblastoma tissue with respect to the normal tissue, while MUC1 and KRT19 are only slightly expressed. Multiple intense signals of each probe were localized in the same nuclear areas throughout the retinoblastoma tissue, with high background noise.
Conclusion
These findings suggest that CD133 is a potential biomarker for the staging and diagnosis of retinoblastoma in Filipino cancer patients. However, further optimization of the hybridization procedures is recommended.
Retinoblastoma
;
Biomarkers
;
In Situ Hybridization
7.Diagnosis and study of viral diseases by using in situ hybridization method.
Korean Journal of Infectious Diseases 1993;25(3):189-194
No abstract available.
Diagnosis*
;
In Situ Hybridization*
;
Virus Diseases*
8.Detection of Human Papillomaviruses DNA in Genital Wart - like Lesions.
Joo Hyun CHOI ; Kyoung Chan PARK ; Un Cheol YEO ; Jeong Aee KIM ; Yoo Shin LEE ; Kye Yong SONG
Korean Journal of Dermatology 1990;28(4):445-449
In situ hybridization using biotinylated HPV(Human papillomaviruses) probes was performed to detect HPV DNA in 24 patients with genital wart-like lesions. The lesions were divided into two groups, with or without dysplastic changes histologically. We could detect HPV6/11 in 13 of 17 lesions(76.5%) without dysplastic changes. HPU16/18 was detected in a case with dysplastic changes. HPV6/ll was also detected in a case considered to be misdiagnosed as bowenoid papulosis. Oncogenic HPV such as HPV16/18 was found in one of histologically splastic lesions(14.3%).
Condylomata Acuminata*
;
DNA*
;
Humans*
;
In Situ Hybridization
9.Expression of Dazla Gene in the Development of Mouse Ovary.
Chang Suk SUH ; Yong Beom KIM ; Seung Yup KU ; Byung Chul JEE ; Young Min CHOI ; Jung Gu KIM ; Shin Yong MOON ; Jin Yong LEE ; Seok Hyun KIM
Korean Journal of Fertility and Sterility 2002;29(3):159-166
No abstract available.
Animals
;
Female
;
In Situ Hybridization
;
Mice*
;
Ovary*
10.Use of Fluorescent in Situ Hybridization to Evaluate the Number of Chromosomes in Buccal Smear Cells in Normal Newborn Infants.
Byoung Chan LEE ; Jee Yeon SONG ; So Young KIM ; Hyun Hee KIM ; Seunghoon HAN ; Jong in BYUN ; Wonbae LEE ; Kyong Su LEE
Journal of the Korean Pediatric Society 1995;38(11):1470-1475
No abstract available.
Humans
;
In Situ Hybridization, Fluorescence*
;
Infant, Newborn*
Result Analysis
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