In order to obtain some informations on the nature and relative activity of the phosphatases present in various helminths, biochemical studies have been made in thirteen kinds of worm parasites including the adults and larvae (Fasciola hepatica, Eurytrema pancreaticum, Paramphistomum sp., Taenia solium, Taenia pisiformis, Dipylidium caninum, Diphyllobothrium mansoni, Cysticercus cellulosae, Cysticercus fasciolaris and Sparganum). A comparison based on the analysis of pH-activity curves was made among these helminths. The worm materials were mostly obtained alive from an abattoir and removed from the organs or tissues of the animal hosts naturally infected. Sparganum and Cysticercus cellulosae, however, are collected from the subcutaneous tissue of the patients by surgical removal. The worms thoroughly washed were weighed and transferred with 0.1 M Tris buffer to a chilled glass grinder (Capacity; 15 ml) and homogenized in the cold. The homogenate was centrifuged at 5000 RPM for 30 minutes. The supernatant was pipetted off for determination of the phosphatase activity. Incubation mixtures consisted of 1 ml substrate, 1 ml buffer and 0.5ml extract. The buffers used were Tris (Hydroxymethyl) aminomethane and citric acid monohydrate and the substrate was paranitrophenyl phosphate (1 gm/25 ml). These mixtures were incubated at the temperature of 37 C for 30 minutes in water bath. The absorbance or transferance of mixture was determined colorimetrically by "Spectronic 20 "spectrophotometer at 410 nm against a distilled water blank. The amount of phenol liberated was then calculated from a standard curve using phenol solutions. Controls consisted of unincubated mixtures. The results were deducted from this experiment. The phosphatase activity occurred over all parasitic helminths used in this experiment. In trematodes, pH-activity curves have demonstrated two peaks of phosphatase activity in Fasciola hepatica and Paramphistomum species. However the acid phosphatase activity was predominantly found and the alkaline phosphatase activity was found distinctly to be low in all three species. In Eurytrema pancreaticum, the pH-activity curves displayed two peaks in acid phosphatase activity, one at pH 5.0 and the other pH 9.0. In cestodes, both alkaline and acid phosphatase activity displayed the pH optima 5.0 and 9.0 to 10.0 in the adult tapeworms. However, major activity in the adults is due to the alkaline phosphtases. In contrast to the adults, Cysticercus and sparganum showed the higher activity in acid phosphatases which predominates in the larvae. In all cases of nematodes, the pH optimum for acid phosphatase was 4.0 to 6.0. A preponderance of acid phosphatase activity was shown in the extract of intestine of Ascaris lumbricoides. The aspect that phosphatases are correlated with phosphorylated passage of substances through the cuticle of helminths and may also be involved in carbohydrate metabolism is discussed.
parasitology-helminth-trematoda-cestoda ; Fasciola hepatica ; Eurytrema pancreaticum ; Paramphistomum sp. ; Taenia solium ; Taenia pisiformis ; Dipylidium caninum ; Diphyllobothrium mansoni ; Cysticercus cellulosae ; Cysticercus fasciolaris ; sparganum ; alkaline phosphatase ; acid phosphatase ; biochemistry

It is well established that guinea pig, rabbit and rat are equally susceptible to experimental infection with the Chinese liver fluke, Clonorchis sinensis. However, little work has been done on the studies of the host-parasite relationships between the liver fluke and mouse host. The experiments were undertaken to investigate the susceptibility, development and sexual maturity of Clonorchis sinensis in mouse host. The metacercariae of C. sinensis used in these experiments were isolated from the fish, Pseudorasbora parva by digestion technic. And in all these studies the mice weighing around 20 g were commercially purchased and infected with the metacercariae under slightly narcotized condition with ether. The animals were starved overnight before infection. The mature metacercariae, e.g. 5, 10, 20, 30, 50, 200 were given orally into stomach by means of the polyethylene tubing (intramedic, Clay Adams, Inc.PE-90/S12) respectively to each mouse of groups ranging 4 to l5. After various period of infection, the mouse liver was first opened along the common bile duct and cut into small pieces for collecting and counting the worms. For the histopathological examination of the liver, the tissue specimens were fixed in formalin, sectioned and stained with haematoxylin-eosin. Another experiment was separately set up for the study of egg laying capacity of C. sinensis in mice. The following results were obtained: Mice were successfully infected with metacercariae of C. sinensis in all cases of the groups except the group given 5 and 10 metacercariae, in which the infection was not established in one case of each group. Therefore it is assumed that mice are susceptible to infection with this worm. In an earlier period ot infection, the worms were found mostly in common bile duct and intrahepatic biliary passages with same rate, however in later period, they were seen rather in the intrahepatic biliary passages (73 percent) than in common bile duct and gall bladder. The recovery rate of fully matured adult worms (less than 10 percent) was lower than that of the average recovery rate of the worms in general (22.3 percent). Under same age of infection or even in same host, the size of collected worms showed the great variations. Oral sucker was smaller in size than ventral sucker in the early stage of infection. After 11 days of infection it reversed. The posterior part of body length began to elongate since 5 days after infection, and therefore the ratio of antero-posterior part became l to 3. Fully matured adult worms were only collected after 30 days of infection. The first positive appearance of eggs in feces was on the 17th and 18th day after infection. However, the egg-laying capacity in mouse host seemed to be stabilized since 30 days after infection. Histopathologically, the cystic dilatation of medium to small biliary passages was noted and focal but diffusely scattered necrosis of the liver cells with scarce inflammatory cells was also observed as well as the hepatocellular degeneration, diffuse vascular congestions and adenomatous proliferationof bile duct.
parasitology-helminth-trematoda-Clonorchis sinensis ; mouse ; host-parasite relationship ; worm recovery ; histology ; pathology ; liver

There have been many methods of manual reduction of shoulder dislocation. But each method has its unique complications such as fracture of'humeral neck or shaft as well as vascular and nerve injuries around the shoulder due to excessive pulling and rotation during the maneuver. The present authors devised a method in which the backrest of a chair is used as a fulcrum during manupulative reduction. The dislocated shoulder can be abducted by the backrest rim, when the armpit is placed over the top of the backrest. In this abducted position of the shoulder, minimal adduction force on distal humerus and gentle backward pressure on humeral head are very effective in reducing the anterioly disocated shoulder.
Axilla ; Humeral Head ; Humerus ; Methods ; Neck ; Shoulder Dislocation ; Shoulder

Bilateral acoustic neurofibrornatosis or neurofibrornatosis-2 is characterized by bilateral acoustic neurornas and it is thought to be genetically distinct from the neurohbrornatosis-1. Also called von Recklinghausen's neurofibrornatosis. We report 2 patients with neurofibrornatosis-2. Who showed progressive bilateral hearing loss, unsteady gait and headache.Neuroirnaging studies revealed bilateral cerebellopontine angle rnasses and biopsies confirmed the diagnosis.
Acoustics ; Biopsy ; Cerebellopontine Angle ; Diagnosis ; Gait Disorders, Neurologic ; Hearing Loss, Bilateral ; Humans ; Neurofibromatoses*

OBJECTIVE: Our purpose was to evaluate whether maternal body mass index measured before pregnancy are associated with an increased risk of cesarean delivery. METHODS: Maternal weight and height were prospectively collected on 819 women who delivered between 37 weeks and 42 weeks in the College of Medicine, Catholic University of Taegu-Hyosung. Statistical analysis was done using Chi-square tests, prepregnancy maternal weight and height were used to calculate the body mass index, and its contribution to the risk of cesarean delivery was determined. Women with congenital anomaly and pregestational diabetes were exclude from analysis. RESULTS: The analysis of risk factors for cesarean delivery in the 819 women revealed a decreased risk of cesarean delivery with maternal age < or =20 years and multiparity; increased risk of cesarean delivery was noted with maternal age >30 years. Increase in prepregnancy maternal body mass index and total weight gain were significantly associated with increase in the odds of cesarean delivery. CONCLUSION: The risk of cesarean delivery is associated with incremental changes in maternal weight and body mass index before pregnancy after adjustment for potential confounding factors. Prepregnancy counseling about optimizing maternal weight and monitoring weight gain during pregnancy to decrease the risk of cesarean delivery are supported by this study.
Body Mass Index* ; Counseling ; Female ; Humans ; Maternal Age ; Parity ; Pregnancy ; Prospective Studies ; Risk Factors ; Weight Gain

We performed intra-osseous venography in 16 patients with chondromalacia patellae or patellofemoral osteoarthritis. Of 16 patients, eight patients had chondromalacia patellae and 6 patellofemoral osteoarthritis. Two patients with normal patellae who underwent meniscectomy were used as controls. The results obtained were as follows: 1. Dye congested in the normal patella was disappeared within 2 minutes after injection and extra-osseous drainage pattern disappeared within 1 minute. 2. in chondromaiacia patellae, large amount of dye was spread throughout the entire patella and remained till 10 minutes, while extra-osseous drainage pattern disappeared at about 5 minutes. 3. In patellefemoral osteoarthritis, venous engorgment in patella was similar to that of chondromalacia patellae and extra-osseous drainage pattern disappeared at about 10 minutes. Conclusively, authors thought the patella I.O.V. was one of the significant clinical methods to define the etiological factors of the various patellofemoral disorders such as patellofemoral osteoarthritis and chondromalacia patellae.
Chondromalacia Patellae ; Drainage ; Estrogens, Conjugated (USP) ; Humans ; Osteoarthritis ; Patella ; Phlebography

No abstract available.
Conjunctiva* ; Melanoma*

No abstract available.
Orthopedic Procedures* ; Orthopedics*

No abstract available.
Ossification of Posterior Longitudinal Ligament* ; Spine*

Anisakiasis is a parasitic infectious disease caused by eating raw, infected fish. It causes severe abdominal pain-like acute abdomen, but not fatal. Anisakiasis was firstly reported by van Thiel et al. in 1960 in Netherlands and it has subsequently been reported in the Netherlands, Japan, the United states, Korea as well as other nations. Because Koreans like eating raw fish, the anisakiasis of the stomach was occasionally reported, but the case of severe gastric bleeding was very rare. So we report one case of gastric ulcer bleeding due to gastric anisakis larva proven by endoscopy and gastric resection.
Abdomen, Acute ; Anisakiasis* ; Anisakis ; Communicable Diseases ; Eating ; Endoscopy ; Hemorrhage* ; Japan ; Korea ; Larva ; Netherlands ; Stomach ; Stomach Ulcer* ; United States