BACKGROUND: Although phospholipase C (PLC)-B3 is thought to be a very important enzyme in intracellular signal transduction, the sophisticated and complicated purification steps make it difficult to obtain sufficient amount of protein to study regulation of its activity by G proteins or other proteins. In order to get large amount of PLC-B3 protein, I employed baculovirus expression system which is known to express large amount of functionally active proteins. METHODS: In order to make recombinant baculovirus which expresses PLC-B3 gene, partial cDNA of PLC-B3 which lacked 51 nucleotides was used to make full length PLC-B3 cDNA. By PCR, 5-end sequence of PLC-B3 was ligated into partial rat PLC-B3 cDNA and later cloned into pVL1393 transfer vector to make recombinant baculovirus. This recombinant baculovirus containing PLC-B3 sequence was used to infect Sf9 insect cells. RESULTS: Infection of Sf9 cells with recombinant baculovirus rendered expression of 152 kDa-PLC-B3 protein, which was confirmed by immunoblot assay and PLC activity assay. CONCLUSION: The whole length PLC-B3 cDNA was expressed in Sf9 cells using baculovirus expression system. By using it, homogeneous enzyme is expected to be purified to study precise activation and regulation mechanisms of PLC-B3.
Animals ; Baculoviridae* ; Clone Cells ; DNA, Complementary ; GTP-Binding Proteins ; Insects ; Nucleotides ; Phospholipases* ; Polymerase Chain Reaction ; Rats ; Sf9 Cells ; Signal Transduction ; Type C Phospholipases

No Abstract Available.
GTP-Binding Proteins* ; Receptors, Cell Surface*

No abstract available.
Airway Resistance* ; Oscillometry*

No abstract available.
Child* ; Humans ; Prognosis*

We experienced a case of Rotor syndrome in an 8 year 6 month old boy who presented with icteric sclera and icteric skin on whole body. His clinical and laboratory characteristics were as follows; 1) Jaundice appeared from several months ago before admission without any clinical disturbance. 2) Direct bilirubin was more increased than indirect bilirubin. 3) Plasma indocyanine green (ICG) kinetics test revealed hepatic excretory in this patient that after intravenous injection of a standard dose of ICG (0.5mg/kg), its initial plasma disappearance is decreased, resultin in markedly increased retention of the dye 45 to 50 minutes after administration. 4) Oral cholangiography, ultrasonogram, and computed tomogram of abdomen were normal. 5) Liver biopsy specimen showed no dark brown pigmentations in the hepatic cells and any other pathologic abnormalities. 6) Nearly similar clinical and laboratory findings were observed 26 months later.
Abdomen ; Bilirubin ; Biopsy ; Cholangiography ; Hepatocytes ; Humans ; Hyperbilirubinemia, Hereditary* ; Indocyanine Green ; Infant ; Injections, Intravenous ; Jaundice ; Kinetics ; Liver ; Male ; Pigmentation ; Plasma ; Sclera ; Skin ; Ultrasonography

To elucidate the clinical characteristics-especially its clinical pattern and outcome-of children with encephalopathy, we carried out a retrospective review on medical records of 45 patients who were initially diagnosed as encephalopathy except mumps meningitis, Reye's syndome and neonatal hypoxic ischemic encephalopathy at the Department of pediatrics, Hanyang University children's hospital from Jaunary 1986 to February 1994. The results were summarized as follows: 1) Twenty five were male and twenty were female with male to female ratio 1.3:l. 2) There were no association with death rate and conscious level, abnormal liver function test, and sugar level in CSF on admission. 3) Death rate (33.3%) of group with seizure was higher than that (13.3%) of group without seizure on admission, but there was no statistical significance (p=0.28). 4) Death rate (7.5%) of group (l) which initial therapy started before 12hours was lower than that (43.4%) of group (ll) which initial therapy started after 12hours, but there was no statistical significance (p=0.09). However complete recovery rate (66.7%) of the former group was higher than that (26.7%) of the latter group with statistical significance (p<0.05). Therefore, early diagnosis and treatment before 12hours was essential to recovery and good prognosis in childern with acute encephalopathy.
Child* ; Early Diagnosis ; Female ; Humans ; Hypoxia-Ischemia, Brain ; Liver Function Tests ; Male ; Medical Records ; Meningitis ; Mortality ; Mumps ; Pediatrics ; Prognosis ; Retrospective Studies ; Seizures

BACKGROUND: Compared to condyloma acuminatum, the cause of laryngeal papilloma has not been clearly established. Although mode of viral transmission is not known yet, laryngeal papilloma seems to be caused by same viruses as condyloma acuminatum. OBJECTIVE: This study was done to investigate the HPV types of condyloma acuminatum and laryngeal papilloma and the epidemiologic relationship between these two diseases. METHODS: The polymerase chain reaction (PCR) was done in fifteen cases of condyloma acuminatum and ten cases of laryngeal papilloma. RESULTS: Same types of HPV DNA (HPV6,11) were detected in all cases of condyloma acuminatum and laryngeal papilloma. CONCLUSION: Laryngeal papilloma is considered a pure viral disease caused by HPV 6 or HPVll and closely related to condyloma acuminatum caused by HPV 6, 11.
DNA* ; Human papillomavirus 6 ; Humans* ; Papilloma* ; Polymerase Chain Reaction* ; Virus Diseases

BACKGROUND: Compared to condyloma acuminatum, the cause of laryngeal papilloma has not been clearly established. Although mode of viral transmission is not known yet, laryngeal papilloma seems to be caused by same viruses as condyloma acuminatum. OBJECTIVE: This study was done to investigate the HPV types of condyloma acuminatum and laryngeal papilloma and the epidemiologic relationship between these two diseases. METHODS: The polymerase chain reaction (PCR) was done in fifteen cases of condyloma acuminatum and ten cases of laryngeal papilloma. RESULTS: Same types of HPV DNA (HPV6,11) were detected in all cases of condyloma acuminatum and laryngeal papilloma. CONCLUSION: Laryngeal papilloma is considered a pure viral disease caused by HPV 6 or HPVll and closely related to condyloma acuminatum caused by HPV 6, 11.
DNA* ; Human papillomavirus 6 ; Humans* ; Papilloma* ; Polymerase Chain Reaction* ; Virus Diseases

The treatment of calcaneal fracture has been controvertial because of its difficulty for many years, and now it tended to be concluded with ultimate result of earIy weight bearing. Authors tried a serial treatment of calcaneal fracture with enforcing early weight bearing after a week of elevation, eold compression, and other 3 weeks of cast immobilization. Materials are compised of 21 feet of 18 patients. Of which 12 cases treated by this method have restored normal range of walking ability. Another group of materials treated by open reduction showed delayed recovery.
Clinical Study ; Foot ; Humans ; Immobilization ; Methods ; Reference Values ; Walking ; Weight-Bearing

OBJECTIVES: To evaluated the relation of familial history of alcoholism and plasma level of beta-endorphin, ethanol, beta-endorphin, cortisol and blood glucose were compared in 48 male alcoholics and 29 normal controls. METHODS: Subjects are divided into two groups by family history of alcoholism. Blood samples were obtained before and after 0.75mg/kg of ethanol consumption a 7th admission day. RESULTS: 1) The ratio of family history positive to negative of the patient group was 2 to 1. 2) The age at admission of positive family history group was younger than negative groups. 3) There was no significant difference in change of plasma ethanol level among three groups. 4) There was no significant difference in change of plasma beta-endorphin level among three group. 5) There was no significant difference in change of plasma cortisol level among three groups. 6) There was no significant difference in change of fasting blood sugar level between two patients groups.
Alcoholics ; Alcoholism* ; beta-Endorphin* ; Blood Glucose ; Ethanol ; Fasting ; Humans ; Hydrocortisone ; Male ; Plasma