1.Isolation of GTP binding from bovine brain.
Yeungnam University Journal of Medicine 1993;10(2):360-368
GTP binding protein (G-protein) associated with membrane and involved in signal transduction was isolated from bovine brain, and molecular weight of G protein was observed. As the results, cell membranes were homogenized from bovine brain tissues and proteins of membrane were gained using 1% cholate, and progressed the chromatography. The purification process was performed by step, DEAE-Sephacel, Ulttrogel AcA 34 and heptylamine-Sepharose column chromatography. The chromatographic fractions were confirmed by GTP binding assay and SDS-polyacrylamide gel electrophoresis. Molecular weight of Goa was revealed 39,000 dalton and GR 36,000 dalton. One more step of heptylamine-Sepharose was enforced to purify the GTP binding protein. Finally I gained the GTP binding protein isolated subtype of Goalpha and Gbeta.
Brain*
;
Cell Membrane
;
Cholates
;
Chromatography
;
Electrophoresis
;
GTP-Binding Proteins
;
Guanosine Triphosphate*
;
Membranes
;
Molecular Weight
;
Signal Transduction
2.Multidrug Resistance in Cancer Chemotherapy.
Yeungnam University Journal of Medicine 1996;13(1):11-21
No abstract available.
Drug Resistance, Multiple*
;
Drug Therapy*
3.Brain Tumors in Childhood.
Korean Journal of Pediatrics 2004;47(Suppl 2):S384-S396
No abstract available.
Brain Neoplasms*
;
Brain*
4.The choice of resurfacing flap according to volumetric concept in the lower leg region.
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1992;19(5):878-886
No abstract available.
Leg*
5.Infertility Counseling for Clinicians.
Sook Jung HWANG ; Hye Jung HWANG
Korean Journal of Fertility and Sterility 2006;33(4):207-217
No abstract available.
Counseling*
;
Infertility*
6.The relationship of hyperuricemia to risk factors of atherosclerosis.
Kyeong Soo CHEON ; Hye Won JUNG ; Hye Soon PARK
Journal of the Korean Academy of Family Medicine 1993;14(12):774-786
No abstract available.
Atherosclerosis*
;
Hyperuricemia*
;
Risk Factors*
7.The clinical significance of serum CA125 and CA19-9 levels in endometriosis.
Tae Jung KANG ; Hye Sung MOON ; Kyung Ah JEONG ; Hye Won JUNG ; Jung Ja AHN
Korean Journal of Obstetrics and Gynecology 2000;43(7):1181-1188
OBJECTIVE: Because endometriosis is difficult to diagnose and has a high recurrence rate after treatment, a reliable serum marker of endometriosis is necessary. Therefore, the aim of this study is to measure the serum levels of CA125 and CA19-9 in patients with endometriosis before and after treatment and during recurrence, and to assess the usefulness of these levels in the diagnosis, clinical follow up and prediction of recurrence in endometriosis. METHODS: Eighty-eight patients who visited the department of Obstetrics and Gynecology of Ewha Mokdong Hospital from January 1994 to December 1998 and were diagnosed as endometriosis by laparoscopy or explo-laparotomy were enrolled as subjects. A retrospective analysis of serum CA125 and CA19-9 levels at 1 month before and 3 to 6 months after initiation of treatment was done. RESULTS: The serum CA125 and CA19-9 levels of endometriosis group(81.0+/-252.5, 36.6+/-53.4 ; mean+/-2SD, U/ml) before treatment was significantly higher than control group(11.6+/-12.8, 9.4+/-8.6)(p<0.05). Overall sensitivity rate for CA125, CA19-9 levels and both was 53.4%, 42.9% and 64.3% respectively. The sensitivity rate for endometriosis, stage 3 and 4(85.4%, 55.0%) was significantly higher than that, stage 1 and 2(p<0.05). The serum CA125 level in endometriosis group showed a significant increment according to stages(p<0.05) while the serum CA19-9 level showed an increasing trend(p=0.055) and both levels decreased significantly after treatment(p<0.05). The serum CA125 level was also higher at recurrence after treatment(p<0.05). CONCLUSIONS: The serum CA125 and CA19-9 levels are a useful marker for diagnosing severity of disease, monitoring efficacy of treatment and predicting recurrence in endometriosis.
Biomarkers
;
Diagnosis
;
Endometriosis*
;
Female
;
Follow-Up Studies
;
Gynecology
;
Humans
;
Laparoscopy
;
Obstetrics
;
Recurrence
;
Retrospective Studies
8.Analysis of nucleotides and their derivatives in renal tissue of rat during ischemia by HPLC.
Yeungnam University Journal of Medicine 1992;9(1):90-101
In rat kidney, the changes in concentrations of nucleotides and their derivatives during ischemia induced by renal artery ligation was measured quantitatively with high performance liquid chromatography (HPLC). After the ligation of renal artery for 60minutes, the concentrations of the nucleotides and derivatives to 80.7±18.39 µg (p<0.01); ATP, 307.2±56.68 µg to 47.6±5.95µg (p<0.01); ADP+AMP, 227.1±7.98 µg to 61.4±3.92 µg (P<0.01); NAD+, 217.9±4.49 µg to 126.6±10.44 µg (P<0.01); GTP, 202.5±23.76 µg to 117.7±14.24 µg (P<0.05); GMP, 54.5±9.03µg to 23.7±0.46 µg (p<0.05), and inosine, 16.6±3.45 µg to 7.8±0.87 µg (P<0.05). But hypoxanthine and xanthine were significantly increased from 113.0±15.58µg to 159.7±12.97µg (P<0.05) and from 87.7±6.77µg to 173.1±12.52µg (P<0.01). In ischemic kidney, concentration of ATP was decreased to 39.9% of control at 10 minutes, 19.8% at 30 minutes, and 15.5% at 60 minutes, and ADP+AMP were decreased to 70.3% of control at 10 minutes, 67.3% at 30 minutes, and to 27.0% at 60 minutes, but hypoxanthine and xanthine were increased to 121.5% and 127.1% at 10 minutes, 126.0% and 174.4% at 30 minutes, and 141.4% and 197.3% at 60 minutes. Total adenosine nucleotides were decreased to 20.3% of control during 60 minutes of ischemia, but hypoxanthine and xanthine were increased to 157.5% of control. These results suggest that the changes in the concentration of nucleotides and their metabolic derivatives are useful indices of the extents of tissue ischemia in rat kidney.
Adenosine
;
Adenosine Triphosphate
;
Animals
;
Chromatography, High Pressure Liquid*
;
Chromatography, Liquid
;
Guanosine Triphosphate
;
Hypoxanthine
;
Inosine
;
Ischemia*
;
Kidney
;
Ligation
;
Nucleotides*
;
Rats*
;
Renal Artery
;
Xanthine
9.Multidrug resistance and cytotoxicity of anticancer drug by verapamil in cisplatin resistant human stomach cancer cell.
Seong Kweon SON ; Jung Hye KIM
Yeungnam University Journal of Medicine 1992;9(1):75-89
The development of multidrug-resistant tumor cell population is a major problem in the chemotherapy of human cancer. These cells are often cross resistant to unrelated drugs and the precise mechanisms of multidrug resistant phenotype of tumor cells has not been fully elucidated. Cisplatin resistant tumor cell (SNU-1/Cis₅) was induced from human stomach cancer cell line (SNU-1) in vitro. Growth profiles of survival cells were observed during 5 days by thiazolyl blue (MTT) assay. To investigate the cross resistance of various anticancer drugs in SNU-1 and SNU-1/Cis5, We compared the value of IC₅₀-drug concentration at 50% survival of control and gained relative resistances (RR). The RR for SNC-1/Cis₅ were as follows; vinblastine, > 43.0; epirubicin, 22.9; dactinomycin, 16.0; etoposide, 15.0; vincristine, 9.2; adriamycin, 5.7; aclarubicin, 5.3. But 5-fluorouracil, methotrexate, daunorubicin have not cross resistance with cisplatin. Resistant inhibition values of 10µM verapamil for SNU-1/Cis₅ were as follows; vincristine, 13.1; epirubicin, 10.0; etoposide, 6.3; vinblastine, 4.4; dactinomycin, 3.6; daunorubicin, 2.4. Membrane proteins of 51,400 and 81,300 daltons were identified by radioiodination with SDS-PAGE, which might represented the drug resistance.
Aclarubicin
;
Cell Line
;
Cisplatin*
;
Dactinomycin
;
Daunorubicin
;
Doxorubicin
;
Drug Resistance
;
Drug Resistance, Multiple*
;
Drug Therapy
;
Electrophoresis, Polyacrylamide Gel
;
Epirubicin
;
Etoposide
;
Fluorouracil
;
Humans*
;
In Vitro Techniques
;
Membrane Proteins
;
Methotrexate
;
P-Glycoprotein
;
Phenotype
;
Stomach Neoplasms*
;
Stomach*
;
Verapamil*
;
Vinblastine
;
Vincristine
10.Effects of Growth Hormone Therapy in Prader-Willi Syndrome.
Journal of Korean Society of Pediatric Endocrinology 2000;5(1):52-59
PURPOSE: Growth hormone(GH) has not only growth promoting effect but also various metabolic effects. We evaluated GH effects by anthrometric data, biochmical data, electrolytes and simple CT in patients with Prader-Willi syndrome. METHODS: Nine children with Prader-Willi syndrome(PWS) were studied. The children were treated with GH(0.6U/kg/week) for 6 months. Before and after therapy we measured height, weight, waist, hip, and thigh. Blood sampling for eletrolytes, HgA1C, lipid profiles and other biochemistry were done in all patients before and after therapy. We also compared fat distribution with scan. RESULTS: Height standard deviation (SD) score increased from -0.7 to -0.5 and weight SD score decreased from 5.3 to 4.9. Body mass index(BMI) decreased from 28.2kg/m2 to 27.2kg/m2. But the changes in height, weight and BMI were not significant statistically. The waist/hip ratio decreased from 1.04 to 0.97(P<0.05), Thigh circumference had been decreased from 58.2+/-21.7cm to 49.9+/-6.9cm insignificantly. The visceral fat were decreased from 7,613+/-1,760 to 5,022+/-1,533 after GH therapy, and thigh muscle mass was increased from 6,358+/-1,616 to 7,175+/-2,155 (P<0.05). Total cholesterol and triglyceride decreased and HDL cholesterol increased after therapy although they were insignificant statistically. There were no differences in electrolytes, HgA1C, other biochemistry(Ca, P, protein, albumin, BUN, Cr) before and after therapy. CONCLUSION: In children with PWS, waist/hip ratio and fat mass were reduced and muscle mass was increased after GH therapy. There was tendency that total cholesterol and triglyceride decreased and HDL cholesterol increased after therapy. We confirmed that GH therapy had not only growth promoting effect but also metabolic effect on lipid and protein metabolism in children with PWS.
Biochemistry
;
Child
;
Cholesterol
;
Cholesterol, HDL
;
Electrolytes
;
Growth Hormone*
;
Hip
;
Humans
;
Intra-Abdominal Fat
;
Metabolism
;
Prader-Willi Syndrome*
;
Thigh
;
Triglycerides