1. Using the kidney of the rabbit weighing approximately 1.5kg the LDH isozymes, LDH-l through 5, were analyzed on the acetate strip by electrophoresis and it was found that the LDH-3 was the least active isozyme followed by the LDH-2, -4, -1, and -5 in increasing order of activity both in that cortex and the medulla, showing a specific distribution pattern in the two tissues. 2. The difference in the isozymic distribution pattern between the cortex and the medulla was confirmed by separating the H-and M-LDH by DEAE-cellulose, the ratio of M-/H-LDH being l. 441t the medulla and 3. O3 in the cortex, showing more active anaerobic metabolism in the latter than in the former. 3. The total activity of LDH in the 20% homogenate of the total kidney tissue was 10. 55 units/ml the cortical 2.98 units/ml. and the medullary 7. 37 unite/ml. 4. In the unilaterally nephrectomized rabbit. the cortex of the remaining kidney showed increased activities of both H- and M-LDH, but the medullary H-LDH decreased, resulting in increases of the ratio M-,H-LDH both in the cortex and in the medulla 5. In the unilaterally pedicle-clamped rabbit, LDH of the clamped kidney medulla decreased while that of the cortex increased, and LDH of the unclamped kidney showed elevated activity both in the cortex and in the medulla, yielding an increase in the ratio of M-/H-LDH. 6. The specific difference in distribution pattern of LDH igozymes between the cortex and the medulla of the rabbit kidney and an increase of the ratio of M-/H.LDH resulting from functional compensation following unilateral nephrectomy or pedicle clamping have been briefly discussed.
Compensation and Redress ; Constriction ; DEAE-Cellulose ; Electrophoresis ; Isoenzymes ; Kidney Medulla ; Kidney* ; Metabolism ; Nephrectomy ; Oxidoreductases*

No abstract available.
Hepatitis B, Chronic* ; Hepatitis, Chronic*

No abstract available.
Hepatitis, Chronic* ; Korea* ; Lamivudine*

No abstract available.
Hepatitis B* ; Hepatitis*

No abstract available.
Dementia*

No abstract available.
Arteriovenous Fistula* ; Hospitals, General* ; Humans ; Reoperation*

No abstract available.
Aged* ; Humans ; Hypertension*

The orthopaedic treatment regarding to the residual deformity secondary to epiphyseal plate injury is the most important thing. Author particularily interested in the microscopic finding upon the repair of damaged transepiphyseal fracture of the distal femoral end of growing rabbits. Experimentally this type of fracture is produced surgically and fixation is achieved by two Kirshner wires; one Kirshner wire is inserted obliquely through the metaphysis. A total of 10 growing rabbits were fed with normal diet, and each 5 rabbits were psacrified by 8 and 10 weeks after the surgical procedure respectively and histological change is examined. No rabbit had infection for those experiment. The results of observation were summarized as follows:1. The ruptured epiphyseal plate due to osteotomy through epiphyseal plate was found to be healed by new bone and fibrotic tissue, not by cartilage regeneration. 2. The epiphyseal plate in the small part of fracture fragments revealed as follows; a. The arrangement of cartilage zones revealed marked irregularity. b. The form and the character of cartilage cells revealed an abnormality such as the hyperophy or the atrophy of cells or both. c. The necrotic area revealed at the several places. d. The thickness of epiphyseal plate revealed more narrowing and irregularity. 3. The effect on the kirschner wires were seen as follows; a. There was no evidence of cartilage regeneration on the margin of the damaged epiphyseal plate. due to Kirschner-wire insertion b. The adjacent tissues around the both kirschner Wires revealed pretty heavy new bone formation.
Atrophy ; Bone Wires ; Cartilage ; Congenital Abnormalities ; Diet ; Femur ; Growth Plate ; Osteogenesis ; Osteotomy ; Rabbits ; Regeneration

The purpose of this study is to observe the changes in 99mTc-MDP uptake of the tibia during blood-flow and skeletal phases and determine the clear borderline between both phases. Serial 99mTc-MDP uptake measurements were performed, as control, in ten matured rabbits with skin incision only and in thirty matured rabbits with fractures of middle and distal one-third of tibia, at ten minutes, thirty minutes, one hour, two hours and four hours after injection of bolus. A value in uptake ratio was calculated by measurements of 99mTc-MDP uptake at each observation time for analogous regions of normal and fractured tibia applied with or without tourniquet. The results obtained were as follows: 1. Group I (skin incision only in ten rabbits) 1) Without tourniquet, the mean 99mTc-MDP uptake ratio tibia was value of 1.05 at ten minutes, 0.97 at thirty minutes, 0.99 at one hour, 1.01 at two hours, 0.97 at three hours and 1.08 at four hours. 2) With tourniquet, the mean 99mTc-MDP uptake ratio of tibia was value of 0.61 at ten minutes, 0.64 at thirty minutes, 0.76 at one hour, 0.98 at two hours, 0.94 at three hours and 1.05 at four hours. 2. Group II (fracture with K-wire fixation in twenty rabbits) 1) Without tourniquet, the mean 99mTc-MDP uptake of tibia was value of 0.73 at ten minutes and 0.80 at two hours. 2) With tourniquet, the mean 99mTc-MDP uptake ratio of tibia was value of 0.45 at ten minutes and 0.76 at two hours. 3) There were no significant change of the 99mTc-MDP uptake ratio in two to four hours after fracture whether the tourniquet was applied on or not. 3. In cases with tourniquet application, there were no significant changes of 99mTc-MDP uptake ratio during two to four hours in group I anda II. This experiments suggest the optimal time for evaluation of the skeletal phase is in two hours following the injection of bolus (99mTc-MDP).
Rabbits ; Skin ; Technetium Tc 99m Medronate ; Tibia ; Tourniquets

No abstract available.
Erythroblastosis, Fetal* ; Gene Frequency* ; Infant, Newborn