No abstract available.
Hernia, Inguinal*

No abstract available.
Humans ; Male*

In an attempt to discover the factors contributing to the increased proliferative activity in hepatocytes and subsequent development of HCC, the proliferative activity of hepatocytes was compared with the size of regenerative nodules and HBcAg expression status in the surrounding nontumorous liver of 45 surgically resected hepatocellular carcinomas, including 34 HBV related ones. In the tumor, the difference in proliferative activity and the histological grade was analyzed in terms of p53 gene alteration. The proliferative activity was assessed by immunohistochemical methods using Ki-67 monoclonal antibody. HBcAg expression in the surrounding nontumorous liver correlated with both the inflammatory and proliferative activity of hepatocytes (p<0.05). p53 overexpression was associated with high proliferative activity and aggressive phenotype of tumor. No correlation was observed between the proliferative activity of hepatocytes and the size of regenerative nodules in cirrhosis (p>0.05). p53 overexpression was not evident in surrounding nontumorous liver including cirrhosis. In conclusion, the above results are in line with the view that hepatic carcinogenesis is a mutistep, progressive process. In the initial stage, chronic cellular injury incurred by immumologic reaction against HBcAg seems to play a pivotal role in increased cellular regeneration. However, once transformation of hepatocytes occur the major contributor to tumor growth seems to be alteration in p53 tumor suppresor gene.
Carcinogenesis ; Carcinoma, Hepatocellular* ; Fibrosis ; Genes, p53 ; Hepatitis B Core Antigens* ; Hepatitis B virus ; Hepatocytes ; Liver* ; Phenotype ; Regeneration

No abstract available.
Spinal Cord Injuries* ; Spinal Cord* ; Syringomyelia*

Proliferating cell nuclear ntigen (FCNA) iis a nuclear protein that is syntheaimd in late Gl and S phases of cell cycle and is correlated with the cell proliferative stale. The recent study demonstrated that FCNA functions in 13NA replication. The present study evaluated proliferetive iindices (PI) for the assessment of tumor proliferation and for investigating prognostic significancx, in cervical tumors. lmmunohiatoehemical PCNA staining was perfurmed in formalin-fixed paraffin-embedded cervical tissues via the avidin-biotin-complex immunoperoxidase methad. Mean PI was 36.03+/-5.14% in normaI controls, as compared to 66.19+/-11.36% in cerviml intraepithelial neoplasia. and 63.19+/-10.94% in invasive cervical cancer. Our results showed no significant correlation between Pll and histological type. Among invasive cervical cancer (24 cases), PI waa 64.43+/-10.94% in squamoua cell carcinoma and 59.00+/-4.10% in adenocarcinoma. There was no eipiifiant relationship between Fl and clinical etage, and between PI and lesion size. This study auggeste that Pl may not serve as a new prognostie factor in cervical tumors.
Adenocarcinoma ; Cell Cycle ; Nuclear Proteins ; Proliferating Cell Nuclear Antigen* ; S Phase ; Uterine Cervical Neoplasms

In order to clarify the preneoplastic nature of large regenerative nodules without dysplastic change, we analysed the clonality of hepatocellular carcinomas (HCCs) and large nodules, diameter > or =0.5 cm, of cirrhotic liver by X-linked human androgen receptor (HUMARA) gene assay, using the principle of random X chromosome methylation and inactivation in female. Ten cases of HCC and 5 cases of large nodules without dysplasia from 9 female patients were selected. All the tumors, large nodules and paired normal control cells were selectively microdissected from deparaffinized hematoxylin and eosin stained slides. Genomic DNA was isolated and digested with HhaI. Polymerase chain reaction(PCR) amplication of the HUMARA locus was performed using 32P-a-dCTP containing PCR mixtures. The PCR amplified products were separated by gel electrophoresis and analysed by autoradiography. Nine HCCs from 8 patients were monoclonal and 1 case was polyclonal and the remaining 1 case was not polymorphic at the HUMARA locus. The HCC case which showed polyclonality contained many inflammatory cells. All the large nodules were polyclonal by HUMARA assay. These results suggest that all or most of the cells composing the large regenerative nodules without dysplasia are polyclonal. This assay may be informative for the differentiation between regenerative and preneoplastic nodules in cirrhotic liver and the size of nodule may be not important in hepatocarcinogenesis.
Autoradiography ; Carcinoma, Hepatocellular* ; DNA ; Electrophoresis ; Eosine Yellowish-(YS) ; Female ; Fibrosis ; Hematoxylin ; Humans ; Liver ; Methylation ; Polymerase Chain Reaction ; Receptors, Androgen ; X Chromosome

To understand better the complex natural course of HBV infection, the expression patterns of HBcAg and HBsAg in the liver of 51 inactive serum HBsAg carriers (24 CPH and 27 NPD) were studied by immunohistochemical methods. The inactive serum HBsAg carriers were devided into 3 groups by the following expression patterns of serum HBeAg/anti-HBe status and tissue HBcAg and HBsAg. Pattern A (18 cases) : HBeAg+, cHBcAg+ (94.4%), mHBsAg+ (61.1%), pATTERN B (14 cases) : anti-HBe+, nHBcAg+, cHBsAg+, Pattern C (19 cases) : anti-HBe+, HBcAg-, cHBsAg+ (89.5%). There were no significant differences between CPH and NPD, lthough the core free pattern was more common in the latter. The cHBcAg was expressed in 17 of 18 (94.4%) HBeAg seropositive cases but only one of 33 cases with serum anti-HBe, suggesting that the cHBcAg is intimately related to HBeAg. Since the inactive HBsAg carriers also expressed cHBcAg and/or mHBsAg, the necro-inflammatory activity of HBV infected liver is assumed to depend on the host immune response rather than their presence alone

Renal and perinephric abscess are uncommon diseases with a multitude of clinical presentations. In past morbidity and mortality were high rate because of delay in diagnosis. Diagnosis and treatment approaches were challenged recently by newer imaging modalities as ultrasonography and CT scanning and by interventional uroradiology. A clinical observation was made in 9 patients with renal abscess and 1O patients with perinephric abscess admitted in the Dept. of Urology, Kyung Hee University Hospital during the period from Jan. 1981 to Jan. 1986. The following results were observed: 1. Among 19 patients, 11 patients were women and their age was ranged from 22 to 68 yrs old with mean of 44.5 yrs. In 12 cases, Lt. kidneys were involved. 2. In 5 patients who had positive urine culture, E. coli was recovered in 3 cases, and klebsiella sp. and staph. epldermidis were recovered in one case, respectively. Blood culture was positive in one case. Among 14 cases performed pus culture, bacteria was recovered in 8 cases: E. coli in 4 cases staph. aureus in 2 cases, and staph. epldermidis and peptococcus in one case, respectively. 3. In 15 cases, diagnosis could be confirmed by ultrasonography, but CT scan was needed in 4 cases to confirm the nature of lesion. The common findings of ultrasonogram were mixed echogenic mass and echolucent mass found in 8 cases and 7 cases, respectively. 4. Among 19 cases, 1O patients underwent open drainage, 5 cases were treated by antibiotics only, and 3 cases were managed successfully by closed drainage.
Abscess* ; Anti-Bacterial Agents ; Bacteria ; Diagnosis ; Drainage ; Female ; Humans ; Kidney ; Klebsiella ; Mortality ; Peptococcus ; Suppuration ; Tomography, X-Ray Computed ; Ultrasonography ; Urology

Neonatal hepatitis(NH) and congenital extrahepatic biliary atresia(BA) are two major causes of neonatal cholestasis. The method of therapeutic trials for each disease is essentially different. Nonetheless it is very difficult to differentiate these diseases histologically, since most of the hepatic changes are mutual in both of them. This study is to aimed to find out major differences between the two by scoring various histological parameters. A total of 63 consecutive liver biopsies taken from 54 patients with suggested NH and BA were examined by applying morphometric scoring system. The detailed clinical histories, laboratory data including serology for HBsAg and TORCH infection and radiologic operative findings were reviewed. Among 54 patients, 27 were diagnosed as NH and 20 as BA. In two cases, features of both diseases were coexistent. The pathological diagnosis was not compatible with the final diagnosis in 5 cases(10.7%). In all of these 5 cases, biopsy had been performed at the age of one to two months. The seropositivity for TORCH was 59.3%(16.27) in NH, but 25.0%(5/20) in BA. Serum AST, ALT and alpha-fetoprotein values were higher in NH, and total bilirubin in BA. Of various histological parameters, scores of portal fibrosis, bile duct and ductular proliferation and bile thrombi were much higher in BA, and at the age of less than 2 months, extramedullary hemopoiesis(EMH) was found much more frequently in NH. Giant cell transformation of hepatocytes(GCT) was more commonly observed in NH. The numbers of GCT and EMH were particulary plentiful when the patients' sera were positive for HBsAg or TORCH. These results indicate that portal fibrosis, biliary proliferation and bile thrombi are the three major histologic features of BA, and therefore erroneous histological diagnosis may ensue when scores of those features are low as in some early BA.
Infant, Newborn ; Humans ; Biopsy

Clinical observation was done on 40 cases of renal cell carcinoma, who have been admitted to the Department of Urology, Kyung Hee University Hospital from January, 1978 to January, 1988. The results were obtained as follows. 1. Among 40 patients, there were 22 men and 18 women, with peak incidence at the sixth decade. 2. Hematuria was the most frequent symptom at the presentation followed by flank pain and abdominal mass. 3. On laboratory examinations, 4 patients had anemia and 2 patients had erythrocytosis. Among 7 patients with hypercalcemia, 2 patients had bone metastasis. 6 patients showed abnormal liver function and liver function became normalized after nephrectomy in 5 of them. 4. Comparing to pathologic stage, accuracy of CT scan in determining of stage was 82.3 %, 5. 3 year-survival rate was 75% in patients with stage I, and 33% in stage II, 40% in stage III. 6. In 14 patients, there were metastasis to lung, lymph node and bone in order of frequency.
Anemia ; Carcinoma, Renal Cell* ; Female ; Flank Pain ; Hematuria ; Humans ; Hypercalcemia ; Incidence ; Liver ; Lung ; Lymph Nodes ; Male ; Neoplasm Metastasis ; Nephrectomy ; Polycythemia ; Tomography, X-Ray Computed ; Urology