1.Increased Growth of Human Leukemic HL-60 Cells by Dimethyl Sulfoxide and Dimethylformamide.
Korean Journal of Immunology 1999;21(3):285-289
A recent study has proposed that dimethyl sulfoxide (DMSO) increases the number of HL-60 cells in high cell density conditions by inhibiting cell density-dependent apoptosis. We observed that dimethylformamide (DMF), a DMSO-related polar compound, also increased the concentration of HL-60 cells. The effective dose range of DMSO and DMF was 0.5-1% and 0.2-0.6% respectively. DMF, like DMSO, inhibited density-dependent apoptosis of HL-60 cells. The flow cytometric PKH26 cell proliferation assay showed DMSO and DMF actively increased cell division. However, the difference in the distribution of cell cycle phase was not noted between the control and the DMSO- or DMF-treated HL-60 cultures. Finally, DMSO and DMF stimulated HL-60 growth even in low density conditions. These results suggest that DMSO and OMF at appropriate concentrations increase the number of HL-60 cells by both apoptosis inhibition and cell division augmentation.
Apoptosis
;
Cell Count
;
Cell Cycle
;
Cell Division
;
Cell Proliferation
;
Dimethyl Sulfoxide*
;
Dimethylformamide*
;
HL-60 Cells*
;
Humans*
2.Effect of 1,25 dihydroxy-vitamin D3 and physiological inducers on the expression of interleukin-1?gene in the human myeloid leukemic cells.
Jong Suk OH ; In Chol KANG ; Jin JEONG ; Boo Ahn SHIN ; Myung Ja SONG ; Hyun Chul LEE
Journal of the Korean Society for Microbiology 1993;28(1):69-79
No abstract available.
Humans*
3.Expression of antigens during HL-60 cell differentiation.
Jong Suk OH ; In Chol KANG ; Phil Youl RYU ; Boo Ahn SHIN ; Hyun Chul LEE
Journal of the Korean Cancer Association 1991;23(3):536-543
No abstract available.
HL-60 Cells*
;
Humans
4.An Experimental Study on the Role of Blood Vessels in the Formation of Peritumoral Abnormal MR Signal Intensity.
Man Chung HAN ; Heung Sik KANG ; Chu Wan KIM ; Ji Hye KIM ; Chol Woo KIM
Journal of the Korean Radiological Society 1994;31(5):933-939
PURPOSE: To assess the role of blood vessels in the formation of peritumoral abnormal signal intensity which exaggerates the size of malignant tumor on MR images. MATERIALS AND METHODS: We performed MR-microangiographic-pathologic correlation using implanted VX-2 carcinoma in 16 rabbit thighs 1-28 days after tumor implantation. The shape and distribution of abnormal vessels were analyzed on microangiography and on histologic examination in correlation with peritumoral abnormal signal intensity on MR images. RESULTS: Dilated peritumoral blood vessels gave rise to irregular, tortuous tumor vessels penetrated into the tumor. With the tumor growth, hypervascular tumor vessels in peritumoral area and central avascular areas were increased. These hypervascular areas on microangiography were corresponded with abnormal signal intensity on MR images. CONCLUSION: Hypervascularity could be a cause of peritumoral abnormal signal intensity which exaggerates the size of experimentally induced malignant musculoskeletal tumors on MR images.
Blood Vessels*
;
Thigh
5.A Case of Transseptal Approach to Carotid Artery Stenting in Right Internal Carotid Stenosis.
Woong Chol KANG ; Young Sup YUN ; Donghoon CHOI ; Won Heum SHIM
Korean Circulation Journal 1998;28(8):1409-1413
Although the carotid endarterectomy presently represents the standard therapeutic approach for most patients with significant carotid artery stenosis, a percutaneous transluminal angioplasty (PTA) with stenting has become an alternative method for treating patients with co-mobid conditions, particulary coronary artery disease. A PTA with stenting has the potential for being safer, less traumatic, more cost-effective, and useful in patients at high surgical risk. As well, they are not limited to the cervical carotid artery. But it is reported that carotid angioplasty by femoral approach is difficult to do in 1 - 2% of patients with carotid stenosis due to abnormal origin of carotid artery or occlusion of femoral arteries. We succeeded in PTA with stenting of tight stenosis of right internal carotid artery through the transseptal approach in case of a sharply angled right brachiocephalic artery take-off from the aorta. The transseptal approach can be used for PTA with stenting in case of problems with femoral approach.
Angioplasty
;
Aorta
;
Arteries
;
Carotid Arteries*
;
Carotid Artery, Internal
;
Carotid Stenosis*
;
Constriction, Pathologic
;
Coronary Artery Disease
;
Endarterectomy, Carotid
;
Femoral Artery
;
Humans
;
Stents*
6.In vitro infection of murine macrophages with salmonella typhimurium and listeria monocytogenes.
Hyun Chul LEE ; Jung Sook PARK ; In Chol KANG ; Sung Sik PARK
Journal of the Korean Society for Microbiology 1991;26(6):499-505
No abstract available.
Listeria monocytogenes*
;
Listeria*
;
Macrophages*
;
Salmonella typhimurium*
;
Salmonella*
7.Terminal Latency Index of the Median Nerve: Normal Values and Relation to Carpal Tunnel Syndrome.
Sa Yoon KANG ; Jay Chol CHOI ; Ji Hoon KANG
Journal of the Korean Neurological Association 2004;22(6):609-612
BACKGROUND: The terminal latency index (TLI) is a calculated value that adjusts the distal motor latency for the terminal distance and the proximal motor nerve conduction velocity. The purpose of this study is to evaluate the sensitivity of the median TLI in the diagnosis of carpal tunnel syndrome (CTS) and compare it with other electrophysiological parameters. METHODS: We prospectively studied 32 patients with nerve conduction studies using a conventional surface technique in the median and ulnar nerves. The distal distance for the CMAP was measured in 5 cm using Oh's techniques. Based on the clinical diagnosis, 32 patients (59 hands) were judged to have CTS. Control data were obtained from 40 healthy hands utilizing identical techniques. RESULTS: The mean TLI was 0.24 +/- 0.04 in the CTS group and 0.30 +/- 0.02 in the control group (P<0.01). The 32 patients had a mean age of 57 years (SD +/- 13 years; 73% women). Twenty subjects with an average age of 46 years (SD +/- 15 years; 65% women) were evaluated for control data. The calculated lower limit of normal from the control group (mean-2SD) was 0.26. The sensitivity of the TLI was 73%. The TLI was statistically better than the median motor distal latency and sensory nerve conduction velocity in the second digit-wrist segment. In four patients from the CTS group, the TLI was the only abnormal electrophysiological parameter. CONCLUSIONS: The median TLI is an easy, inexpensive, highly informative test and is therefore extremely useful in the diagnostic work-up of patients with CTS.
Carpal Tunnel Syndrome*
;
Diagnosis
;
Hand
;
Humans
;
Median Nerve*
;
Neural Conduction
;
Prospective Studies
;
Reference Values*
;
Ulnar Nerve
8.Striopallidodentate Calcinosis: Association with Hypothyroidism?.
Sa Yoon KANG ; Jay Chol CHOI ; Ji Hoon KANG
Journal of the Korean Neurological Association 2007;25(2):206-208
Bilateral striopallidodentate calcinosis involves calcification of the basal ganglia and dentate nuclei of the cerebellum. On occasion it may be associated with either hypoparathyroidism or pseudohypoparathyroidism. We report a 52-year-old woman with enduring dysarthria. She had bilateral calcification of the basal ganglia, thalamus, and dentate nuclei on a brain CT. She also had hypothyroidism. Familial study including her son and daughter was negative. We emphasize the need to evaluate the thyroid function in patients with specific intracranial calcifications.
Basal Ganglia
;
Brain
;
Calcinosis*
;
Cerebellum
;
Dysarthria
;
Female
;
Humans
;
Hypoparathyroidism
;
Hypothyroidism*
;
Middle Aged
;
Nuclear Family
;
Pseudohypoparathyroidism
;
Thalamus
;
Thyroid Gland
9.Two Cases of Hypertensive Encephalopathy Involving the Brainstem.
Sa Yoon KANG ; Jay Chol CHOI ; Ji Hoon KANG
Journal of Clinical Neurology 2007;3(1):50-52
Hypertensive encephalopathy is a medical emergency whose clinical manifestations are usually associated with bilateral parieto-occipital lesions. Predominant brainstem edema without accompanying occipital lesions is rare in hypertensive encephalopathy and usually occurs in patients with secondary hypertension. We describe the clinical and radiological features of two patients with reversible hypertensive brainstem encephalopathy. Both patients had chronic renal failure, but the extensive neuroimaging abnormalities revealed few clinical features of brainstem involvement. The clinical findings and neuroimaging abnormalities resolved once the hypertension was treated.
Brain Stem*
;
Edema
;
Emergencies
;
Humans
;
Hypertension
;
Hypertensive Encephalopathy*
;
Kidney Failure, Chronic
;
Neuroimaging
10.Combined Facial and Contralateral Trochlear Nerve Palsy in a Patient with Diabetes Mellitus.
Chol Jay CHOI ; Sa Yoon KANG ; Ji Hoon KANG
Journal of the Korean Neurological Association 2006;24(2):166-168
There is an increasing number of reports that the lesion site in isolated cranial neuropathies may be the brainstem. The authors describe a diabetic patient with peripheral type facial palsy and concurrent trochlear palsy. Magnetic resonance imaging showed only a small pontine infarction responsible for the facial palsy. Multiple cranial nerve palsies seen in this patient might be a manifestation of multiple acute small infarcts involving both the brainstem and its cranial nerve root simultaneously.
Brain Stem
;
Brain Stem Infarctions
;
Cranial Nerve Diseases
;
Cranial Nerves
;
Diabetes Mellitus*
;
Facial Paralysis
;
Humans
;
Infarction
;
Magnetic Resonance Imaging
;
Paralysis
;
Trochlear Nerve Diseases*
;
Trochlear Nerve*