Objective To evaluate the clinical effect of transcatheter arterial chemoembolization (TACE) combined with three-dimension conformal radiotherapy (3DCRT) in treating unresectable primary Hepatic carcinoma(PHCC).Methods 64 cases of PHCC were randomly divided into 2 groups,TACE combined with 3DCRT group (Treatment group) and TACE alone group (control group).34 cases in treatment group were treated with TACE 2 ～3 times at first,after resting for 2-4 weeks,3DCRT treatment was used.30 cases in control group were treated with TACE 2 ～ 3 times only.Results The percentage of tumor regression rate (CR + PR) was 73.5% in treatment group and 36.6% in control group.The difference was significant (P ＜0.01).The percentage of AFP declined was 100% in treatment group and 92.9% in control group,which difference was not significant (P ＞0.05).The cumulative survival rate of 1-,2-and 3-years were 76.5%,58.8% and 41.2% in treatment group,and 56.7%,26.7%,13.3% in control group respectively.There was significant difference between the two groups (P ＜ 0.05).Conclusion The treatment of TACE combined with 3DCRT is an effective and security treatment method for primary Hepatic carcinoma.

Objective To investigate the clinical efficacy and security of intravenous sequential oral voricon -azole treatment of blood diseases combined invasive fungal infections (IFI).Methods Combined blood of patients were reated with invasive fungal infections ,88 patients were randomly divided into the control group and treatment group using sequential treatment , the control group were treated with conventional anti-infective therapy , treatment group based on the use of conventional anti-infective drugs ,plus implementation of voriconazole therapy .The clinical efficacy were compared and adverse drug reactions were observed .Results The total effective rate of the treatment group was 90.91%,which was significantly higher than 70.45%of the control group (χ2 =5.906,P<0.05);the in-fection control in patients treated with time ,continuous medication and incidence of adverse events of the treatment group were significantly lower than those of the control group (t=2.019,P<0.05;t=1.987,P<0.05;χ2 =4.062, P<0.05).Conclusion Intravenous sequential oral voriconazole therapy combined blood invasive fungal infections in patients is safe ,effective,fewer adverse reactions ,which is widely used clinically .

Thyroid associated ophthalmopathy (TAO) is an organ-specific autoimmune-inflammatory disorder,ranking top among the orbital diseases with highest incidences.TAO is characterized by a complex pathogenesis,including glycosaminoglycan and hyaluronic acid deposition,fibrosis of extraocular muscle and adipose degeneration of ocular tissue.The physical manifestations of the disease include periorbital edema,double vision,ophthalmoptosis,ocular dryness,eyelid retraction and on occasion vision loss secondary to optic nerve compression.Selenium evolves in the diversified pathogenesis of TAO.Although the therapeutic effects for TAO of selenium have already been proven,some problems appear to be no clear among the clinicians,such as its optimum dose,duration of the treatment,and safety to date,etc.Nowadays,the mechanism of selenium supplementation in TAO treatment includes neutralization of reactive oxygen,inhibition of HLA-DR expression,inhibition of secretion of TSHR-Ab and TPO-Ab.The aim of the review was to summarize the studies of selenium supplementation theray for TAO.

Objective To express the fusion protein ABD-Fc-IL-2 in eukaryotic cells and detect its biological activity. Methods The target gene SP-ABD-Fc-IL-2 was amplified by direct and overlapping PCR,and then ligated to vector pcDNA3. 1(+).The obtained recombinant plasmid pcDNA3. 1/SP-ABD-Fc-IL-2 was transiently transfected into CHO-S cells to express the fusion protein ABD-Fc-IL-2,which was purified by Protein A beads affinity chromatography. The specificity of the purified fusion protein was detected by Western blot,the biological activity was detected by CTLL-2/MTT cell proliferation colourimetry,and the interaction between ABD fragment and human serum albumin(HSA)was detected by pull down/Western blot. Results The recombinant plasmid pcDNA3. 1/SP-ABD-Fc-IL-2 was constructed correctly as identified by restriction analysis and sequencing. The purified fusion protein ABD-Fc-IL-2 showed a purity of 90% and bound specifically to mouse anti-IL-2 monoclonal antibody with the biological activity of 3. 29 × 108IU/mL. The ABD of fusion protein and HSA bound to each other. Conclusion The eukaryotic fusion protein ABD-Fc-IL-2 had high biological activity,which promoted the proliferation of CTLL-2 cells and maintained the binding ability of ABD fragment to HSA.

Objective To explore the clinical application value of rapid detection method for respiratory tract influenza virus and mycoplasma pneumonia(MP).Methods 386 cases were selected from patients with respiratory infections in our hospital from Janu-ary 2011 to December 2015 in the study,nasopharyngeal secretions A,B influenza virus were detected by gold immunchromato-graphic assay,the Mycoplasma pneumoniae(MP)was detected by rapid culture,and pathogens-IgM were detected by immunofluo-rescence assay.In the end,the two rapid detection detection method were compared with immunofluorescence assay.Results 386 cases of respiratory tract infections patients were detected by immunofluorescence assay,54 cases were found influenza A virus in-fection,the positive rate was 13.59%,37 cases were found influenza B virus infection,the positive rate was 9.59%,61 cases were found MP infection,the positive rate was 15.80%.In detections of influenza A virus infection and influenza B virus infection,im-munchromatographic assay sensitivity were 20.37%,18.92% and specificity were 93.41%,92.77%.In detection of MP infection, rapid culture sensitivity was 37.70% and specificity was 89.85%.Conclusion Rapid detection of respiratory influenza virus,MP infection sensitivity is low,only as a supplementary means of indirect immunofluorescence assay clinically.

Objective To investigate the method of the operation of the tube type,improve the drying efficiency,and control the occurrence of infection in hospital,reduce the energy consumption.Methods Devices with full automatic washing machine washing out the lumen of 600 pieces were divided into control group and two experimental group by random digital table method each group was 200 pieces,respectively,and using dumped water before drying,luminal devices placed in different position and different container among the three groups in 90 ℃ drying 30 minutes after lumen devices to compare the drying effect.Results At the dry temperature 90℃,the same drying time of 30 minutes,the rate of drying qualified of the experimental group 2 was 100.0％ (200/200),which was higher than 82.5％ (165/200) of the experimental group 1,the difference was statistically significant (x2=38.356,P ＜ 0.05);and also higher than 21％ (42/200) of the control group,the difference was statistically significant (x2=261.157,P ＜ 0.05).Conclusions The new drying operation method can improve the drying efficiency of the tube type,and control the risk of inffection in the hospital.

Objective To observe the efficacy and safety of BiPAP ventilator combined with nikethamide for the chronic obstructive pulmonary disease (COPD)complicated with type II respiratory failure.Methods 76 COPD patients complicated with type II respiratory failure were selected as the research subjects,they were randomly divided into two groups by single blind and randomized method,38 cases in each group.The control group was treated with nikethamide therapy,while the observation group used BiPAP combined with mechanical ventilation therapy.The clinical therapeutic effect and adverse reactions were analyzed and compared in the two groups.Results The total effective rate in the observation group was 92.11%,which was significantly higher than 68.42% in the control group (χ2 =10.54,P <0.05).After treatment,the HR and RR of the observation group were (94.28 ±7.62)/min, (13.27 ±4.41)/min,which were significantly decreased than before treatment (t =8.02,8.04,all P <0.05),and which were significantly lower than those in the control group (t =7.54,6.88,P <0.05).After treatment,the pH (7.39 ±0.06),PaO2 (96.31 ±5.44)mmHg,SaO2 (98.42 ±6.87)% in the observation group were significantly increased (t =7.56,7.76,7.45,all P <0.05),PaCO2 (57.36 ±4.98)mmHg was significantly decreased (t =7.54, P <0.05),which had significant differences with the control group (t =6.56,7.02,6.73,7.32,all P <0.05).There was no significant difference in the incidence of adverse reactions between the two groups (χ2 =2.14,P >0.05). Conclusion BiPAP ventilator combined with nikethamide meters has remarkable curative effect and high safety in treatment of COPD patients complicated with type II respiratory failure,which is worthy of clinical reference and popularization.

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