BACKGROUND: Flecainide is an antiarrhythmic agent that is used primarily in the treatment of cardiac arrhythmias. Some evidences also suggest that flecainide can participate in alveolar fluid clearance and inflammatory responses. This experiment was aimed to evaluate the effects of flecainide on sepsis induced acute lung injury in a rat model. METHODS: Rats were treated with subcutaneous infusion of saline or flecainide (0.1 or 0.2 mg/kg/hr) by a mini-osmotic pump. Subcutaneous infusion was started 3 hours before and continued until 8 hours after intraperitoneal injection of saline or endotoxin. Animals were sacrificed for analyses of severity of acute lung injury with wet to dry (W/D) ratio and lung injury score (LIS) in lung and inflammatory responses with level of leukocyte, polymorphonuclear neutrophils (PMNs) and inteleukin-8 (IL-8) in bronchoalveolar lavages fluid (BALF). RESULTS: Flecainide markedly improved dose dependently sepsis induced acute lung injury as analysed by W/D ratio (from 2.24 ± 0.11 to 1.76 ± 0.09, p < 0.05) and LIS (from 3 to 1, p < 0.05), and inflammatory response as determined by leukocyte (from 443 ± 127 to 229 ± 95, p < 0.05), PMNs (from 41.43 ± 17.63 to 2.43 ± 2.61, p < 0.05) and IL-8 (from 95.00 ± 15.28 to 40.00 ± 10.21, p < 0.05) in BALF. CONCLUSIONS: Flecanide improve sepsis induced acute lung injury in rats by controlling inflammatory responses.
Acute Lung Injury* ; Animals ; Arrhythmias, Cardiac ; Bronchoalveolar Lavage ; Flecainide* ; Infusions, Subcutaneous ; Injections, Intraperitoneal ; Interleukin-8 ; Leukocytes ; Lung ; Lung Injury ; Models, Animal ; Neutrophils ; Rats ; Sepsis*

Uterine sarcomas are relatively rare tumors of mesodermal origin that constitute 2-6% of uterine malignancies. Uterine sarcomas include endometrial stromal sarcoma (ESS), leiomyosarcoma (LMS), malignant mixed m llerian tumor (MMMT) of both homologous and heterologous type, pure heterologous sarcomas, blood vessel sarcomas, and lymphoma. Endometrial stromal tumors are divided into three types: 1) endometrial stromal nodule, 2) low-grade stromal sarcoma or endolymphatic stromal myosis, and 3) endometrial stromal sarcoma. The low grade endometrial stromal sarcoma is very rare. It shows occasional local recurrence, which might arise from endometrial stroma, from adenomyosis, and rarely from endometriosis. We have experienced a case of low grade endometrial stromal sarcoma of the uterus in a 54-year-old woman, which is presented with a review of literatures.
Adenomyosis ; Blood Vessels ; Endometrial Stromal Tumors ; Endometriosis ; Female ; Humans ; Leiomyosarcoma ; Lymphoma ; Mesoderm ; Middle Aged ; Recurrence ; Sarcoma ; Sarcoma, Endometrial Stromal* ; Uterus*

No abstract available.
Cartilage*

Purpose: To report a case of contact lens-related infectious keratitis caused by three different bacterial species.Case summary: A 40-year-old man presented with pain and redness in his left eye. His best-corrected visual acuity (BCVA) in the affected eye was 20/25. Slit-lamp examination revealed a 3 × 3 mm corneal epithelial defect with infiltration located 1 mm inferior to the pupil. Following admission, a microbial culture test was performed, and empirical antibiotic therapy was initiated. On the fourth day, Pseudomonas aeruginosa was isolated from the corneal sample and the contact lens, while Serratia marcescens and Stenotrophomonas maltophilia were isolated from the contact lens case. Based on the results of the antibiotic susceptibility tests, 0.5% moxifloxacin, fortified amikacin, and ceftazidime were administered topically and intravenously. The corneal epithelial defect reduced to 1 × 1 mm by the eleventh day of admission. After two months, BCVA improved to 20/20 with no remaining corneal epithelial defect, although an inactive corneal opacity persisted at the previous ulcer site. Conclusions Contact lens wear can be associated with polymicrobial keratitis involving three distinct Gram-negative bacteria, which may present greater treatment challenges compared to monomicrobial keratitis. Microbial culture testing of the contact lens, its case, and corneal scrapings is essential for identifying the causative organisms and selecting appropriate antibiotic therapy.

Purpose: To report a case of contact lens-related infectious keratitis caused by three different bacterial species.Case summary: A 40-year-old man presented with pain and redness in his left eye. His best-corrected visual acuity (BCVA) in the affected eye was 20/25. Slit-lamp examination revealed a 3 × 3 mm corneal epithelial defect with infiltration located 1 mm inferior to the pupil. Following admission, a microbial culture test was performed, and empirical antibiotic therapy was initiated. On the fourth day, Pseudomonas aeruginosa was isolated from the corneal sample and the contact lens, while Serratia marcescens and Stenotrophomonas maltophilia were isolated from the contact lens case. Based on the results of the antibiotic susceptibility tests, 0.5% moxifloxacin, fortified amikacin, and ceftazidime were administered topically and intravenously. The corneal epithelial defect reduced to 1 × 1 mm by the eleventh day of admission. After two months, BCVA improved to 20/20 with no remaining corneal epithelial defect, although an inactive corneal opacity persisted at the previous ulcer site. Conclusions Contact lens wear can be associated with polymicrobial keratitis involving three distinct Gram-negative bacteria, which may present greater treatment challenges compared to monomicrobial keratitis. Microbial culture testing of the contact lens, its case, and corneal scrapings is essential for identifying the causative organisms and selecting appropriate antibiotic therapy.

Purpose: To report a case of contact lens-related infectious keratitis caused by three different bacterial species.Case summary: A 40-year-old man presented with pain and redness in his left eye. His best-corrected visual acuity (BCVA) in the affected eye was 20/25. Slit-lamp examination revealed a 3 × 3 mm corneal epithelial defect with infiltration located 1 mm inferior to the pupil. Following admission, a microbial culture test was performed, and empirical antibiotic therapy was initiated. On the fourth day, Pseudomonas aeruginosa was isolated from the corneal sample and the contact lens, while Serratia marcescens and Stenotrophomonas maltophilia were isolated from the contact lens case. Based on the results of the antibiotic susceptibility tests, 0.5% moxifloxacin, fortified amikacin, and ceftazidime were administered topically and intravenously. The corneal epithelial defect reduced to 1 × 1 mm by the eleventh day of admission. After two months, BCVA improved to 20/20 with no remaining corneal epithelial defect, although an inactive corneal opacity persisted at the previous ulcer site. Conclusions Contact lens wear can be associated with polymicrobial keratitis involving three distinct Gram-negative bacteria, which may present greater treatment challenges compared to monomicrobial keratitis. Microbial culture testing of the contact lens, its case, and corneal scrapings is essential for identifying the causative organisms and selecting appropriate antibiotic therapy.

Purpose: To report a case of contact lens-related infectious keratitis caused by three different bacterial species.Case summary: A 40-year-old man presented with pain and redness in his left eye. His best-corrected visual acuity (BCVA) in the affected eye was 20/25. Slit-lamp examination revealed a 3 × 3 mm corneal epithelial defect with infiltration located 1 mm inferior to the pupil. Following admission, a microbial culture test was performed, and empirical antibiotic therapy was initiated. On the fourth day, Pseudomonas aeruginosa was isolated from the corneal sample and the contact lens, while Serratia marcescens and Stenotrophomonas maltophilia were isolated from the contact lens case. Based on the results of the antibiotic susceptibility tests, 0.5% moxifloxacin, fortified amikacin, and ceftazidime were administered topically and intravenously. The corneal epithelial defect reduced to 1 × 1 mm by the eleventh day of admission. After two months, BCVA improved to 20/20 with no remaining corneal epithelial defect, although an inactive corneal opacity persisted at the previous ulcer site. Conclusions Contact lens wear can be associated with polymicrobial keratitis involving three distinct Gram-negative bacteria, which may present greater treatment challenges compared to monomicrobial keratitis. Microbial culture testing of the contact lens, its case, and corneal scrapings is essential for identifying the causative organisms and selecting appropriate antibiotic therapy.

OBJECTIVE: The purpose of this study was to investigate the correlation between the cervicographic diagnoses and histologic diagnoses in patients with ASCUS cytologic diagnosis according to The Bethesda System. METHODS: This study was performed in outpatient clinic of Department of Obstetrics and Gynecology at Dankook University Medical Center from January 1, 2000 to July 31, 2000. Of the 120 patients who underwent both conventional Papanicolaou cervical cytologic test and cervicographic examination, 30 cases with ASCUS cytologic diagnosis were analyzed. Cervicographic diagnoses were compared with histologic diagnoses as a reference standard. RESULTS: Followings are the results summarized. 1. Age distribution was between 26 and 62. The mean age was 43.1 years. 2. The mean gravidity was 4.5 and the mean parity was 2.5. 3. There were 19 (63.3%) cervicographic diagnoses of Negative, 6 (20.0%) of Atypical, and 5 (16.7%) of Positive. Of the 5 Positive diagnoses, there were 3 cases of P0 and 2 cases of P1. 4. There were 1 (3.3%) histologic diagnosis of WNL, 23 (76.7%) diagnoses of cervicitis, 3 (10.0%) of LSIL, and 3 (10.0%) of HSIL. 5. Of the 19 cervicographic diagnoses of Negative, there were 17 histologic diagnoses of cervicitis, and 2 of LSIL. Of 6 cases of Atypical, there were 4 histologic diagnoses of cervicitis and 2 of HSIL. Of 5 cases of Positive, there were 3 histologic diagnoses of cervicitis, 1 of LSIL, and 1 of HSIL. 6. Cervicography for detecting LSIL or worse yielded sensitivity of 33.3%, specificity of 87.5%, positive predictive value of 40.0%, and negative predictive value of 84.0%. CONCLUSION: There was no statistical significance because of small data. Cervicography does not seem to replace conventional Papanicolaou cervical cytologic screening test or play an adjunctive role for detection of LSIL or worse in patients with ASCUS on cervical cytology. But a multicenter study with large data will be required to get the proper conclusion.
Academic Medical Centers ; Age Distribution ; Ambulatory Care Facilities ; Diagnosis ; Female ; Gravidity ; Gynecology ; Humans ; Mass Screening ; Obstetrics ; Parity ; Sensitivity and Specificity ; Uterine Cervicitis

BACKGROUND: The oral ulcer is a common oral disorder, but the precise etiology remains elusive despite of intensive clinical, immunological, hematological and microbiological investigations. OBJECTIVE: The purpose of this study was to examine oral ulcers for the detection of HSV DNA by using PCR and to characterize clinical features of HSV DNA positive cases. METHODS: Specimens collected with cotton swabs and saliva from 48 cases of oral ulcers were examined for HSV DNA by PCR and Southern blot hybridization. RESULTS: 1. HSV DNA was detected in 8 of 48(16.7%) cotton swabbed specimens of oral ulcers and saliva by PCR and Southern blot hybridization. 2. Clinical features of HSV DNA positive oral ulcers were a. predominently located in buccal mucosa. b. mall sized(<3mm), multiple(> or =5) lesions. 3. In Behcet's disease, 2 of 7(28.6%) cases of oral ulcers were positive for HSV DNA CONCLUSION: PCR is an useful and accurate method for the detection of HSV DNA from cotton swabbed specimens of oral ulcers.
Blotting, Southern ; DNA ; Herpes Simplex* ; Mouth Mucosa ; Oral Ulcer* ; Polymerase Chain Reaction* ; Saliva ; Simplexvirus*

BACKGROUND: Recent restriction enclonuclease analysis studies hsve revealed that MCV DNA can be classified into two major types, designated MCV-1 and MCV-2, by th:ir restriction enzyme cleavsge patterns. In earlier reports of MCV DNA analysis, MCV-2 was the main virus type found in genital lesions. However many recent studies cienied the relationship between virus type and anatomical distribution. OBJECTIVE: The purpose of this study was to examine the ratio of MCV-l to MCV-2 in Korean isolates of MCV DNA and the relationship between MCV subtypes and with clinical features such as anatomical location, age, sex, numiber of lesions, and atopic dermatitis. METHODS: MCV DNA extrated from 112 cases of Korean patients waa examined by restriction endonuclease analysis using Brtm HI. RESULTS: 1. MCV-1 was found in 108 of 112 (96.4%) patients and MCV-2 in of 112 (3.6%) patients. The ratio of MCV-1 to MCV-2 wss 28:1. 2. There was no significant ciprrelation between MCV subtypes and the age, sex, number of lesions, atopic dermatitis, and anatoimic loction. 3. Lesions induced by MCV-1 MCV-2 were indistinguishable on the brsis of size and form. CONCLUSION: This study showis that the ratio of MCV-1 to MCV-2 was 28:1 in Korean molluscum contagiosum patients and there was no relationship between MCV subtyies and lesional morphology or snatomical distribution.
Dermatitis, Atopic ; DNA ; DNA Restriction Enzymes ; Humans ; Molecular Epidemiology* ; Molluscum contagiosum virus* ; Molluscum Contagiosum*