1.Evaluation of microparticle enzyme immunoassay for the measurement of serum T3.
Korean Journal of Clinical Pathology 1992;12(4):433-437
No abstract available.
Immunoenzyme Techniques*
2.Detection and clinical significances of the occurrence of endogenous LH surge with enzyme immunoassay and fluoroimmunoassay.
Jong Kwan JUN ; Shin Yong MOON ; Yoon Seok CHANG
Korean Journal of Obstetrics and Gynecology 1991;34(7):961-971
No abstract available.
Fluoroimmunoassay*
;
Immunoenzyme Techniques*
4.The incidence of Chlamydia trachomatis infection in women of reproductive age in Hanoi explored by the technique of enzym immunoassay
Journal of Practical Medicine 2003;425(5):12-14
338 subjects at the age of 17-49 were undergone EIA test. Results showed an increasing trend in Chlamydia trachomatis infection. Clinical manifestations were not detected. The incidence is highest at the age of 17-26 (14.68%), 17-36 (10.67%). In the groups of genital tract inflamation, of uterine neck, of vaginitis the incidence reaches 2.7%, 12.71% and 12.50% respectively
Chlamydia trachomatis
;
Immunoenzyme Techniques
;
Women
5.Evaluation of Genedia HCV Rapid for Rapid Detection of Antibody to Hepatitis C Virus.
Sang Khoo LEE ; Woo Hyun LIM ; Seung Jung KEE ; Jung Won SONG ; Jong Hee SHIN ; Soon Pal SUH ; Dong Wook RYANG
Korean Journal of Clinical Pathology 1998;18(4):620-623
BACKGROUND: Hepatitis C virus (HCV) is a major agent of transfusion-associated non-A, non-B hepatitis. The best method for prevention of HCV infection through transfusion is blood donor screening using reliable diagnostic tools. Enzyme immunoassays (EIA) for detection of HCV antibody were developed, but it required equipment and time consuming. In emergency situation such as massive bleeding, HCV screening of blood donor needs more simple, rapid and reliable method. Recently, for rapid detection of HCV antibody, Genedia HCV Rapid assay was developed. To evaluate the usefulness of this assay, comparative studies with third generation anti- HCV EIA and two HCV confirmatory tests (Genedia HCV Confirm 4.0 and Chiron RIBA HCV 3.0 SIA) were performed. METHODS: A total of 156 sera (106 positive and 50 negative), screened by second generation IMx anti-HCV assay (Abbott 2.0; Abbott Laboratories, U.S.A.), were examined with Genedia HCV Rapid (Green Cross, Korea) and Genedia HCV ELISA 3.0 (Green Cross, Korea). The discrepant sera between Genedia HCV Rapid and Genedia HCV ELISA 3.0 were confirmed by both Genedia HCV Confirm 4.0 and Chiron RIBA HCV 3.0 SIA. RESULTS: The concordance rates of Abbott 2.0 vs Genedia HCV Rapid, Abbott 2.0 vs Genedia HCV ELISA 3.0, and Genedia HCV Rapid vs Genedia HCV ELISA 3.0 were 88.4%, 89.7%, and 96.1%, respectively. The sensitivity, specificity, positive predictive value and negative predictive value of Genedia HCV Rapid for Genedia HCV Confirm 4.0 were 97.7%, 100%, 98.9%, and 97.0%, respectively, and for Chiron RIBA HCV 3.0 SIA 97.8%, 98.5%, 98.9%, and 97.0%, respectively. Of discrepant 6 sera between Genedia HCV Rapid and Genedia HCV ELISA 3.0, 2 were positive by Genedia HCV Confirm 4.0, and 3 positive by Chiron RIBA HCV 3.0 SIA. However, 14 negative sera by both Genedia HCV rapid and Genedia HCV ELISA 3.0, which were all positive by Abbott 2.0, were all negative by two confirmatory tests. CONCLUSIONS: These data show that Genedia HCV Rapid could be used in emergency blood donor screening for HCV antibody detection.
Blood Donors
;
Emergencies
;
Enzyme-Linked Immunosorbent Assay
;
Hemorrhage
;
Hepacivirus*
;
Hepatitis C*
;
Hepatitis*
;
Humans
;
Immunoenzyme Techniques
;
Mass Screening
;
Sensitivity and Specificity
6.Evaluation of the Usefulness of Anti-Cyclic Citrullinated Peptide Antibodies Measured by an Automated Enzyme Immunoassay.
Hye Ran KIM ; Jeong Whan SHIN ; Jeong Nyeo LEE
Journal of Laboratory Medicine and Quality Assurance 2005;27(1):183-188
BACKGROUND: Rheumatoid arthritis (RA) is the most common autoimmune rheumatic disease, but sensitive and specific test for its diagnosis is lack. This study evaluated the analytical performance and diagnostic role of a new automated ELISA anti-cyclic citrullinated peptide (anti-CCP) antibody test. METHODS: Anti-CCP antibody test was done with the enzyme-linked immunosorbent assay (ELISA) in serum samples from 49 RA patients and 104 non-RA patients, and 51 healthy subjects. Serum pools were used to determine its precision and linearity. The optimal cut-off values were determined by the receiver-operator characteristics (ROC) curve. The rheumatoid factor (RF) by turbidimetry was also assayed in every samle and the results were compared to anti-CCP for sensitivity and specificity. RESULTS: The total imprecision (CV%) was 4.8%, 7.6% for serum pools with low (mean concentration: 2.7 U/mL) and high (mean concentration :82.2 U/mL) concentration, respectively. Linearity data were acceptable (R2=0.9907). At each optimal cut-off value, the sensitivity of anti-CCP was higher than that of RF (81.6 % vs 69.4%), but statistical significance was not defined. Specificity of anti-CCP was higher than that of RF (95.5% vs 75.5%, p<0.001). A combination of anti-CCP and RF increased sensitivity and specificity to 87.7%, 98.0%, respectively. Nine of 15 (60.0%) sera from RF negative RA patients were positive for anti-CCP. CONCLUSIONS: Anti-CCP ELISA antibody test, we examined on a fully automated enzyme immunoassay, is easy to assay in routine laboratory, and showed good analytical performance. And anti-CCP antibody test also showed higher diagnostic specificity than RF. So, anti-CCP antibody may be useful serologic marker for diagnosis and monitoring of RA, if performed concomitantly with RF assay.
Antibodies*
;
Arthritis, Rheumatoid
;
Diagnosis
;
Enzyme-Linked Immunosorbent Assay
;
Humans
;
Immunoenzyme Techniques*
;
Nephelometry and Turbidimetry
;
Rheumatic Diseases
;
Rheumatoid Factor
;
Sensitivity and Specificity
7.Enzyme Amplification, A Method Applied to Provide An ELISA for Studying Anti-Hib-Ps Antibodies in Children.
Journal of the Korean Pediatric Society 1997;40(2):173-180
PURPOSE: Enzyme immunoassay (EIA) is now a widely used technique. We have described the application of enzyme amplification (sensitive ELISA) in the field of immunoassays of pediatric population. There are two issues with the sensitive ELISA. First is that one can minimize the serum volume, an important concern for pediatricians. The second is the problem of background signal. We demonstrate that it is possible to develop EIAs of high sensitivity and detectability with using very small volume of infant's sera immunized with Hib-PRP vaccine. METHODS: Monoclonal Abs HG11, HP6016, HK2, and KL1 specific for human IgG1, IgG2, C , and C were used. The mAb OAK-1 specific for a subfamily of V I L chains (V Ia), the mAbs KB13 and B12 specific for human V II and V III L chains were also used respectively. Adults were immunized with Hib-CRM vaccine. Immune serum was obtained 4 to 8 wk after immunization. Twenty infants received Hib-CRM vaccine at 2, 4, and 6 month of age and blood samples were obtained at 7 month old. The amount of anti-Hib-PS Ab expressing a V subgroup or V was determined by sandwich type immunoassays using conventional substrate. The amount of the enzyme immobilized to the well was determined with para-nitrophenyl phosphate substrate. A standard ELISA was performed but different substrate (lyophilized NADPH) and amplifier (alcohol dehydrogenase and diaphorase) were used to develop color in final step for enzyme amplification method. RESULTS: We get the dose-response curves obtained using the conventional and amplified detection methods in the anti-PRP Ab assay. The sensitivities of the two assay methods were compared. We can increase the sensitivities four to sixteen folds and minimize the infant's sera volume to perform varing anti-PRP antibody assays. To obtain the advantages of increased sensitivity, any background is minimized by using noncontaminated reagents. CONCLUSIONS: It is possible to develop EIAs of high sensitivity and detectability with using very small volume of infant's sera with using enzyme amplification system (sensitive ELISA).
Adult
;
Antibodies*
;
Child*
;
Enzyme-Linked Immunosorbent Assay*
;
Humans
;
Immunization
;
Immunoassay
;
Immunoenzyme Techniques
;
Immunoglobulin G
;
Indicators and Reagents
;
Infant
;
Oxidoreductases
8.A Simple Outline of Methods for Protein Isolation and Purification.
Endocrinology and Metabolism 2017;32(1):18-22
At the summer workshop of the Korean Endocrine Society held in 2016, some examples of protein experiments were discussed in the session entitled “All about working with proteins.” In contrast to what the title suggested, it was unrealistic to comprehensively discuss all protein analytical methods. Therefore, the goal was to outline protein experimental techniques that are useful in research or in bench work. In conversations with clinicians, however, I have always felt that researchers who do not engage in bench science have different demands than those who do. Protein research tools that are useful in bench science may not be very useful or effective in the diagnostic field. In this paper, I provide a general summary of the protein analytical methods that are used in basic scientific research, and describe how they can be applied in the diagnostic field.
Chromatography
;
Education
;
Immunoenzyme Techniques
;
Methods*
;
Molecular Imaging
9.A Measurement of Rubella Antibodies among Korean Children by Enzyme-linked Immunosorbent Assay.
Ki Young LEE ; Jun Hee SUL ; Young Nam LEE ; Chang Hee KIM ; Joo Deuk KIM
Yonsei Medical Journal 1981;22(1):41-48
We measured the degree of immunity of 326 Korean children to rubella virus by enzyme-linked immunosorbent assy (ELISA). They were admitted to Pediatrics Unit of Yonsei Medical Center between April and July, 1980 with various illnesses excluding rubella disease. Among the 326 tested, 172 cases gave a positive titer of antibodies (mainly, IgG and IgM antibodies) and 127 had IgG antibody against rubella virus antigen. These represented 52.8% and 39.0%, respectively, of the total number of children tested. There was no significant difference in the rate of positivity between sex, but the positive rate increased as the age increased. The antobody titers of positive individuals to rubella virus were higher among the older children. Results and a brief outline of the ELISA method for serodiagnosis of rubella in clinical use will be discussed.
Adolescent
;
Age Factors
;
Antibodies, Viral/analysis*
;
Child
;
Child, Preschool
;
Enzyme-Linked Immunosorbent Assay*
;
Female
;
Human
;
Immunoenzyme Techniques*
;
Infant
;
Infant, Newborn
;
Korea
;
Male
;
Rubella/immunology
;
Rubella virus/immunology*
10.Development of the Enzyme Immunoassay for the Detection of Anti-HSV-2 Antibody with HSV-2 Specific Monoclonal Antibody.
Chung Gyu PARK ; Jae Won PARK ; Dae Joong KIM ; Jinhee KIM ; Eung Soo HWANG ; Hyun Moo LEE ; Ai Young LEE ; Chang Yong CHA
Korean Journal of Infectious Diseases 1999;31(4):309-316
BACKGROUND: The serological diagnosis of herpes simplex virus type 2 (HSV-2) infection has pitfalls, in that most of the antibodies against HSV-2 cross-react with HSV-1 and the prevalence of HSV-1 infection is high, especially in Korea. In this study, we tried to establish the serological diagnostic method, which could detect and measure the specific antibodies against HSV- 2 by competitive immunofluorescent staining method as well as competitive ELISA based on the specific monoclonal antibody, MH2-7. METHODS: Immunofluorescent staining and western blot analysis were used to characterize the antigens recognized by MH2-7. Competitive immunofluorescent staining (IF), competitive enzyme immunoassay (ELISA), and western blot analysis were used to detect specific antibodies against HSV-2 in patients' sera. RESULTS: In western blot analysis, the sera from two of six patients clinically diagnosed as genital herpes showed characteristic band patterns, which have been known to be compatible with HSV-2 infection. In competitive immunofluorescent staining, only the sera from the two patients clinically diagnosed as genital herpes and with characteristic band pattern showed competition with MH2-7 monoclonal antibody. The dilution range of the serum showing specific competition was between 1:10 and 1:80. Competitive ELISA was also performed and evaluated as the diagnostic efficacy as ELISA has been known to be advantageous over IF staining in mass screening. The result showed linear dose-response relationship for the patient's sera in inhibition of the reactivity of MH2-7. CONCLUSION: We suggest that the competitive immunofluorescent staining method and competitive ELISA based on the specific monoclonal antibody MH2-7 is a simple, accurate, and precise method, which can be used in serological diagnosis of HSV-2 infection.
Antibodies
;
Blotting, Western
;
Diagnosis
;
Enzyme-Linked Immunosorbent Assay
;
Herpes Genitalis
;
Herpesvirus 1, Human
;
Herpesvirus 2, Human*
;
Humans
;
Immunoenzyme Techniques*
;
Korea
;
Mass Screening
;
Prevalence