1.Evaluation of microparticle enzyme immunoassay for the measurement of serum T3.
Korean Journal of Clinical Pathology 1992;12(4):433-437
No abstract available.
Immunoenzyme Techniques*
2.Detection and clinical significances of the occurrence of endogenous LH surge with enzyme immunoassay and fluoroimmunoassay.
Jong Kwan JUN ; Shin Yong MOON ; Yoon Seok CHANG
Korean Journal of Obstetrics and Gynecology 1991;34(7):961-971
No abstract available.
Fluoroimmunoassay*
;
Immunoenzyme Techniques*
3.The incidence of Chlamydia trachomatis infection in women of reproductive age in Hanoi explored by the technique of enzym immunoassay
Journal of Practical Medicine 2003;425(5):12-14
338 subjects at the age of 17-49 were undergone EIA test. Results showed an increasing trend in Chlamydia trachomatis infection. Clinical manifestations were not detected. The incidence is highest at the age of 17-26 (14.68%), 17-36 (10.67%). In the groups of genital tract inflamation, of uterine neck, of vaginitis the incidence reaches 2.7%, 12.71% and 12.50% respectively
Chlamydia trachomatis
;
Immunoenzyme Techniques
;
Women
5.A Simple Outline of Methods for Protein Isolation and Purification.
Endocrinology and Metabolism 2017;32(1):18-22
At the summer workshop of the Korean Endocrine Society held in 2016, some examples of protein experiments were discussed in the session entitled “All about working with proteins.” In contrast to what the title suggested, it was unrealistic to comprehensively discuss all protein analytical methods. Therefore, the goal was to outline protein experimental techniques that are useful in research or in bench work. In conversations with clinicians, however, I have always felt that researchers who do not engage in bench science have different demands than those who do. Protein research tools that are useful in bench science may not be very useful or effective in the diagnostic field. In this paper, I provide a general summary of the protein analytical methods that are used in basic scientific research, and describe how they can be applied in the diagnostic field.
Chromatography
;
Education
;
Immunoenzyme Techniques
;
Methods*
;
Molecular Imaging
6.Change of Surface Carbohyd rate during Trans differentiation of Retinal Pigment Epithelial Cell.
Hee Seong YOON ; Sung Chur MOON ; Woo Jin JEUNG ; Hae Il PARK ; Young Hyun YOO
Journal of the Korean Ophthalmological Society 2000;41(4):838-846
This study was conducted to study the changes of cell surface carbohydrates during transdifferentiation of retinal pigment epithelial(RPE)cells. RPE cells were cultured from adult pig eyes. Surface carbohydrates of RPE cells from 1st, 3rd, 5th, 7th and 9th passages were assayed by lectin histochemistry and enzyme immunoassay. Changes in binding affinities to the lectins employed were demonsrated during trasdifferentiation of RPE cell. Whereas binding affinities of ConA, ECL, PNA, WGA, and UEA-I decreased graudally as the number of culture passage increased, binding properties to LCA, STL and DBA fluctuated depending on the number of passages. The results demonstrate changes of surface carbohydrates of RPE cell during trasdifferentiation. We suggest that changes of surface carbohydrates of RPE cell during trasdifferentiation may be close relations with the functional changes during transdifferentiation.
Adult
;
Carbohydrates
;
Epithelial Cells*
;
Humans
;
Immunoenzyme Techniques
;
Lectins
;
Retinaldehyde*
7.Comparison of Three Third-generation Anti-HCV Enzyme Immunoassay Tests.
Hee Soon CHO ; Jin Young MOON ; Chae Hoon LEE ; Kyung Dong KIM
Yeungnam University Journal of Medicine 1998;15(1):143-150
The aim of this study was to evaluate domestic enzyme immunoassay(EIA) kit ?LG HCD 3.0?(LG) for the detection of antibody to hepatitis C virus(anti-HCV) in comparision with Axsym HCV version 3.0(Axsym), Cobas Core anti-HCV EIA(Cobas). Cobas kit shows better clear distinction between positive and negative by signal/cutoff ratio(S/C), but it also reveal relatively high false positive rate. The concordance rate of test results between LG and Axsym was 96.2%, between LG and Cobas was 95.5%, and total agreement between three EIA kit was 93.9%. LG were relative poor distinction between positive and negative results, but it could be applied clinically as a screening tool for hepatitis C in general population. The S/C of one false negative result by LG was 0.91, and false positive were less than 4.0, therefore we concluded it is necessary to confirm by immunoblotting assay when S/C were between 0.8 and 4.0.
Hepacivirus
;
Hepatitis C
;
Immunoblotting
;
Immunoenzyme Techniques*
;
Mass Screening
8.Effectiveness of Supplemental Enzyme Immunoassay Using Abbott IMx HCV Kit for the Anti-HCV Positive Donors.
Heung Bum OH ; Yoo Sung HWANG ; Young Hee CHO ; Doo Sung KIM ; Sang In KIM
Korean Journal of Blood Transfusion 1997;8(2):11-21
BACKGROUND: Only 39% was the positive predictive value of anti-hepatitis C virus (HCV) antibody test done by Korean Red Cross. Supplemental enzyme immunoassay (EIA) by another EIA kit may be also effective for reporting the more correct result to donors, instead of expensive supplemental immunoblot test. METHODS: All repeatedly reactive blood samples by EIA from 16 regional blood centers were retested for anti-HCV antibody by Abbott IMx HCV kit and LG HCD CONFIRM immunoblot kit. Presence of viral RNA was also confirmed using Amplicor HCV TEST kit from 180 samples, which were proportionately selected according to supplemental EIA and Immunoblot results. RESULTS: Of 2,211 repeatedly reactive samples, 909 samples (41%) were reactive and 1,302 (59%) samples were non-reactive with IMx HCV kit. 81% of reactive samples also showed positive pattern on the LG HCD CONFIRM strips and 79% of 1,302 samples showed negative pattern. RNA positivity was estimated 66% and 17% in Abbott IMx HCV positive and negative samples respectively, and 72%, 6%, 20% in LG HCD CONFIRM positive, indeterminate and negative samples respectively. CONCLUSION: HCV RNA positivity in positive samples by Abbott IMx HCV or LG HCD CONFIRM was not statistically significant (z=0.57 < 1.96, alpha=0.05). RNA detection rate by Abbott IMx HCV or LG HCD CONFIRM among HCV RNA positive samples, which was estimated as 73%, 70% respectively, was also statistically insignificant (z=0.375 < 1.96, alpha=0.05). So, it seems to be a good and economical practice that donors are notified of anti-HCV antibody results after supplemental EIA test using Abbott IMx HCV kit.
Humans
;
Immunoenzyme Techniques*
;
Red Cross
;
RNA
;
RNA, Viral
;
Tissue Donors*
9.Confirmation of HCV Positivity for Indeterminate Donors in Anti-HCV Antibody Immunoblot Assay among Blood Donors.
Youn Jung CHO ; Dong Hee SEO ; Byung Gap HWANG ; Dong Jip KIM ; Nam Hyun CHUNG
Korean Journal of Blood Transfusion 2001;12(2):237-244
BACKGROUND: In Korean Red Cross, recombinant immunoblot assay (RIBA) has been used for the confirmatory test of HCV positive units since 1995. To certify the HCV infection in blood donors who showed the 'indeterminate result on the RIBA test, this study was performed. METHODS: Three enzyme immunoassay (EIA) kits (LG HCD 3.0, DONG-A HCV 3.0, and ORTHO HCV 3.0)and RNA detection method were employed to evaluate infection state of 135 samples of the 'indeterminate in the RIBA test. RESULTS: The 52.6% of the samples showed the same test results with three EIA kits. Fifteen samples (11.1%) were HCV RNA positive with RT-PCR-hybridization technique. Among 15 samples of HCV RNA positive, 13 (86.7%), 13 (86.7%), and 14(93.3%) of samples were positive in LG HCD 3.0, Ortho HCV 3.0 and Dong-A HCV 3.0 EIA, respectively. In the analysis of RIBA band reaction, HCV RNA positivity were correlated with core14, core518, and 897 antigen. However, among 64 samples which react with core antigen only, five samples (7.8%) were HCV RNA positive. CONCLUSION: Based on the results of the present study, it is recommend that the HCV RNA test be used as a method of confirmatory test in order to notify exact HCV positivity status to blood donor who showed indeterminate RIBA result.
Blood Donors*
;
Humans
;
Immunoenzyme Techniques
;
Red Cross
;
RNA
;
Tissue Donors*
10.Latex agglutination inhibition test(UCG-slide test) and monoconal antibody - based enzyme immunoassay test (RAMP test) in the diagnosis of ectopic pregnancy.
Hyun Taik SHIH ; Hyuck Dong HAN ; Jung In BAI ; Young Jin LEE ; Sang Won HAN ; Dong Soo CHA ; Dae Hyun KIM
Korean Journal of Obstetrics and Gynecology 1993;36(7):2204-2207
No abstract available.
Agglutination*
;
Diagnosis*
;
Female
;
Immunoenzyme Techniques*
;
Latex*
;
Pregnancy
;
Pregnancy, Ectopic*