Quantitative determination of immunoglobulin was used in the diagnosis of prostatitis in seminal fluid and serum by radial immunodiffusion method. The study was performed in 11 normal healthy men and 20 prostatitis patients and they were compared with each other. Significant elevation of IgA in seminal fluid of patients with prostatitis was obtained.
Diagnosis ; Humans ; Immunodiffusion ; Immunoglobulin A ; Immunoglobulins* ; Male ; Prostatitis*

Determination of immunoglobulin in prostatic secretion and serum by radial immunodiffusion method was used in the diagnosis of prostatitis. The study was performed in 16 normal healthy men and 45 prostatitis patients. The results were as follows; 1 Significant elevation of IgG and IgA in Prostatic fluid of patients with prostatitis was obtained. 2. Ratio of IgA in prostatic secretion to immunoglobulin in serum was increased in patients with prostatitis than normal healthy men.
Diagnosis ; Humans ; Immunodiffusion ; Immunoglobulin A ; Immunoglobulin G ; Immunoglobulins* ; Male ; Prostatitis*

PURPOSE: The purpose of this study is to observe the salivary immunoglobulin level in whole saliva of infected patients and also to investigate the changes of immunoglobulin level according to its management. MATERIALS AND METHODS: Thirty infected patients who have been admitted to the dept. of oral and maxillofacial surgery of Pusan National University Hospital have been selected as subjects and we analysed the changes of immunoglobulin level of 1.5~3.0ml of unstimulated whole saliva collected throughout four times; the day before treatment, the first day after treatment, the third day after treatment and the day before discharge. We also compared them with immunoglobulins in whole saliva that was collected from 4 normal persons as control group. In radial immunodiffusion technique with BACKMAN(Array 360 system, McLean, USA), level of immunoglobulins was analyzed. RESULTS: The isotypes of Ig that have been found in saliva of normal persons were IgG, IgA, IgM and IgE and their mean level was 8.23, 36.41, 4.38, and 2.38 respectively. In the infected patients before the treatment, the level of IgG, IgA was remarkably higher than that of normal persons, however we could not find the difference on the level of IgM, IgE. As the infection was healing, the level of IgG, IgA was decresing significantly.
Busan ; Humans ; Immunodiffusion ; Immunoglobulin A ; Immunoglobulin E ; Immunoglobulin G ; Immunoglobulin Isotypes* ; Immunoglobulin M ; Immunoglobulins* ; Saliva* ; Surgery, Oral

OBJECTIVE: Systemic Lupus Erythematosus(SLE) is characterized by various autoantibodies to a variety of nuclear antigens. Certain extractable nuclear antigens(ENA) have been served as extremly useful aids in differentiation of clinical subset, diagnostic marker and in the detection of early forms of systemic rheumatic diseases. This study was to employ immunoblot to determine the prevalences of antibodies to ENA in SLE compared with immunodiffusion. METHODS: Sera were obtained from 127 SLE patients. Antibodies to ENA were assessed by double immunodiffusion (DID) and immunoblot method. RESULTS: Using the immunblot method, the prevalences of antibodies to ENA were as follows: The antibody to Sm was 27%, UIRNP 33.6%, Ro 41.8%, La 14%, ribosomal P 14% and Ku 4%. The prevalences of antibodies to ENA by DID were as follows: The antibody to Sm was 15%, RNP 24.5%, Ro 54.3% and La 9.4%. CONCLUSIONS: Compared with immunodiffusion, results using immunoblot showed greater sensitivity in the detection of autoantibodies to ENA in SLE.
Antibodies* ; Antigens, Nuclear ; Autoantibodies ; Humans ; Immunoblotting* ; Immunodiffusion* ; Lupus Erythematosus, Systemic* ; Prevalence ; Rheumatic Diseases

The concentrations of three immunoglobulins (IgG, IgA, and IgM) were studied in sera of 307 healthy children from the age of one month to 16 years. The method used was a single radial immunodiffusion technique, with the use of commercially available antibody agar plates. The results obtained were compared with those found in umbilical cord sera and with the average levels in healthy adults. The average concentration of IgG was lowest at the age of 3 months, i.e. about 40 percent of adult level. At the age of one year the average level of IgG was 50 percent of adult level and approached the adult level at the age of 6~8 years (Fig. 1,4 and 5 and Table 1). In cord sera IgA could only be demonstrated in three neonates out of 20. At the age of one year an average IgA level of about 25 percent of adult level was found and the adult level was reached at age of 12 to 16 years(Fig. 2, 4 and 5, and Table 1). IgM was detected in all cord sera with an average concentration of 15 percent of adult level. At the age of 3 months the IgM values were about 50 percent of adult level and at the age of one year approximately 70 percent(Fig. 3,4 and 5, Table 1).
Adult ; Agar ; Child* ; Humans ; Immunodiffusion ; Immunoglobulin A ; Immunoglobulin G ; Immunoglobulin M ; Immunoglobulins* ; Infant, Newborn ; Umbilical Cord

This study was performed to evaluate the changes of serum IgG4 level in children with allergic diseases. Serum Ige, IgG and IgG4 level were measured using enyzme immunoassay (IgE) and radial immunodiffusion method (IgG and IgG4) in 21 children with bronchial asthma, allergic rhinitis or urticaria Eosinophil counts in peripheral blood and the values of serum IgE were significantly increased in allergic patients. The values of IgG4 in allergic patients were also increased compare to those in controls (24.23mg/dl versus 20.33mg/dl). But the values of IgG4 were not significantly correlated to either IgG or IgE levels. Further studies will be needed for measuring allergen specifie IgE and IgG4 levels considering the onset of diseases, methods and duration of treatment.
Asthma ; Child ; Eosinophils ; Humans ; Hypersensitivity ; Immunoassay ; Immunodiffusion ; Immunoglobulin E ; Immunoglobulin G* ; Rhinitis ; Urticaria

BACKGROUND: To interpret the serotyping data from group A streptococcal infections and their complications, serotyping data from healthy children are essential. In this study, we determined the serotypes of the strains isolated from the elementary school children located in Seoul and Chinju. METHOD: Eighty-two strains of group A streptococci isolated from Seoul and 76 strains from Chinju were serotyped with T typing, serum opacity reaction (SOR), opacity factor (OF) and M typing. T typing was undertaken by slide agglutination after trypsinization of bacteria, SOR and OF typing by microwell technique and M typing by Ouchterlony double immunodiffusion after Lancefield's extraction method. RESULTS: In Seoul T 12 (52.4%), T 3 (9.8%), T 5 (8.5%) and T 27 (8.5%) were frequently encountered. In Chinju T 12 (44.7%), T 28 (13.2%) and T 6 (7.9%) were commonly identified. While M 12 (48.8%) and M 5 (14.6%) were most common in Seoul, M 12 (26.3%), M 22 (14.5%) and M 28 (10.5%) were widely distributed in Chinju. SOR positivity was 15.9% in Seoul and 54.0% in Chinju respectively. CONCLUSIONS: The distributions of serotypes of group A streptococci between the two areas were more or less different. Serotyping of strains from healthy children may be considered as the basic data to understand the epidemiology of bona fide group A streptococcal infections and disease association of virulent strains.
Agglutination ; Bacteria ; Child ; Epidemiology ; Gyeongsangnam-do* ; Humans ; Immunodiffusion ; Seoul* ; Serotyping ; Streptococcal Infections ; Trypsin

We examined the incidence of anti-Ro antibodies with sera from 60 Korean patients with systemic lupus erythematosus(SLE) by double immunodiffusion. Among these 60 sera tested, 31(51.7%) showed evident immunoprecipitations against Ro antigen on the Ouchterlony plates. Several recent studies indieate that the anti-Ro antibody occurs much less frequently in Occidental lupus patients(around 30%) than in Japanese lupus patients (50%). These data may be suggestive that the racial or ethnic baekground might be an important factor determining the serological, and perhaps the clinical features of SLE.
Antibodies* ; Asian Continental Ancestry Group ; Humans ; Immunodiffusion ; Incidence* ; Lupus Erythematosus, Systemic

It is known that antithrombin III is a potent vasodilator and plasmin is a vasoconstrictor, and some patients with a subarachnoid hemorrhage(SAH) develop clinical vasospasm and some patients do not. Under the hypothesis that the development of clinical vasospasm might depend on the difference of the blood level of antithrombin III in each patient with SAH and that the plasmin might have a role in the development of clinical vasospasm, we repeatedly checked the levels of blood antithombin III with a single radial immunodiffusion method and CSF fibrinogen degradation products(FDP : indirect indicator of plasmin activity) with a latex-test(Thrombo-Wellcotest(R)) during the period between 1-4, 5-11 and 12-24 days after a SAH in 29 patients. 10 patients with diseases except those with a SAH were selected as a control group. First, we analyzed the difference of the average of blood antithrombin III and CSF FDP between aneurysmal SAH patients and control patients and then, between patients with clinical vasospasm(8 cases) and patients without clinical vasospasm(21 cases). Secondly, we also analyzed the difference of these data between patients with clinical vasospasm and patients without clinical vasospasm according to the sampling day after a SAH. As a result, there was no statistical difference between the average blood level of antithrombin III in control and in SAH patients(29.06+/-3.04 vs. 25.61+/-6.95, respectively), and in patients with clinical vasospasm and in patients without clinical vasospasm(26.59+/-7.65 vs. 23.67+/-7.40, respectively). The average CSF levels of FDP is higher in SAH patients than in control patients(18.16+/-14.36 vs. 1.00+/-3.16, respectively : p<0.01). It is also higher in patients with clinical vasospasm than in patients without clinical vasospasm. However, there is no statistical significance(28.75+/-9.91 vs. 21.75+/-12.07, respectively : p>0.05). In the analysis of the average CSF levels of the FDP according to the sampling day after a SAH, even though the average levels is higher in patients with clinical vasospasm than in patients without clinical vasospasm(1-4 days : 31.43+/-14.64 vs. 27.33+/-16.24, 5-11 days : 23.75+/-17.68 vs. 18.10+/-16.32, 12-24 days : 32.50+/-13.89 vs. 18.82+/-16.54, respectively), a statistical significant difference was noticed only in levels which were checked between 12 and 24 days after a SAH(p<0.05). This study concludes that the blood level of antithrombin III shows no difference between the control and SAH patients, and patients with clinical vasospasm and patients without clinical vasospasm. Although it suggests a causal relationship between the FDP itself or plasmin in CSF and the development of clinical vasospasm, it does not justify any valid conclusion.
Aneurysm* ; Antithrombin III* ; Cerebrospinal Fluid* ; Fibrinogen ; Fibrinolysin ; Humans ; Immunodiffusion ; Subarachnoid Hemorrhage* ; Vasospasm, Intracranial

This study was performed to investigate associations between serum alpha(1)-antitrypsin(AAT) concentration and radiological categories of coal workers' pneumoconlosis(CWP), between AAT concentration and pulmonary complications such as tuberculosis and emphysema, and to study associations between AAT concentration and FEV(1.0)% in CWP patients, We classified 254 CWP patients in D Hospital into categories of small opacity profusion. And we selected 86 subjects by with or without emphysematous finding in each categories by proportional stratified sampling method. Semm AAT concentrations were quantkated by single radial immunodiffusion method, and the findings of chest radiographs were evaluated by radilogist. The results were as follows: 1. Serum AAT concentrations were not significantly different among groups of radiological categories of small opacities. 2. Complication of emphysema was associated with smoking habits sigmficantlyl(chi square=12.16, p<0,01). And AAT concentraLion was higher in smokers and ex-smokers than in non-smokers. Serum AAT concentration was significantly higher in the cases with emphysema than in the cdses without emphybema{p<0.01). 3. Serum AAT concentration of the group with active pulmonary tuberculosis was significantly higher than with inactive or without: pulmonary tuberculosis group(p<0.1). 4. Serum AAT concentration of the group with low FEV(1.0)% was significantly higher than with high or normal group(p<0.05).
Anthracosis ; Coal* ; Emphysema ; Humans ; Immunodiffusion ; Pneumoconiosis* ; Radiography, Thoracic ; Smoke ; Smoking ; Tuberculosis ; Tuberculosis, Pulmonary