1.Species identification and strain differentiation of bacillus athracis and closely related bacillus species by ouchterlony test using antisera raised against whole bacterial cells of encapsulated and uncapsulated bacilli.
Chul Soon CHOI ; Cheong Hoon PARK ; Sang In CHUNG ; Yong Tae YANG
Journal of the Korean Society for Microbiology 1992;27(5):407-417
No abstract available.
Bacillus*
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Immune Sera*
2.Improved procedure for production of monospecific B.pertussis antisera
Journal of Preventive Medicine 1998;8(2):58-62
The improved procedure for production of monospecific B.pertussis antiserum at the Institute of Vaccines satisfied the technical requirement, sterility specificity, sensibility and stability. Similar results were obtained with monospecific B.pertussis antisera issued by the United Kingdom.
Bordetella pertussis
;
Immune Sera
3.Identification of agglutinogens 1, 2 and 3 by the IVAC monospecific B.pertussis antisera
Journal of Preventive Medicine 2001;11(4):18-21
Monospecific B.pertussis antisera prepared at IVAC, Nha Trang, Da Lat have been used in the identifying testing for the presence of agglutinogens 1, 2 and 3 in B.pertussis strains GL353, 360E, H36, 248, 305, 18323 and in vaccine final bulks L617, L617-636, L624-628, L627-634, L634-636, L613-614. Similar results were obtained with monospecific B.pertussis antisera issued by the United Kingdom.
Virulence Factors, Bordetella
;
Bordetella pertussis
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Immune Sera
4.Use of mycobacterium vaccae as immuno-stimulant in the preparation of H.influenzae type b (Hib) antisera
Journal of Preventive Medicine 2004;14(1):66-70
In order to enhance the capability of antiserum production rabbits, Mycobacterium vaccae was used to stimulate rabbit’s immune system unspecifically before immunization with Haemophilus influenzae type b (Hib). The results showed that only single immunization with Hib antigen: the number of rabbits had response fulfill depended-time requirements (harvested after 1 or 6 months) varied from 30% to 70%, harvesting time for antiserum with fulfilled quantity and quality was 24 weeks (6 months) (2 courses induced immunization). Stimulating rabbit’s immune system by M. vaccae, then inducing rabbit’s immunization with Hib antigen: the number of rabbits had response fulfiling requirements varied from 71.4% to 100%; harvesting time for antiserum with fulfilled quantity and quality was 5 weeks (only after 1 course induced immunization). Sensitivity and specificity of antiserum were unchanged
Mycobacterium
;
Helicobacter pylori
;
Immune Sera
;
Adjuvants, Immunologic
5.Chemical modification of RBC surface antigen with glutaraldehyde crosslinking.
Chae Seung LIM ; Il Tae KIM ; Kyung Ran MA ; Young Kee KIM ; Kap No LEE ; Si Myung BYUN
Korean Journal of Blood Transfusion 1998;9(1):45-49
BACKGROUND: The chemical modification of RBC surface antigen has many advantages for safe transfusion practice. We evaluated the change of antibody reactivity to RBC surface antigen before and after glutaraldehyde crosslinking. MATERIALS AND METHODS: The 10 mL of blood were collected from 20 volunteers and were treated by 2-3% glutaraldehyde at 4degrees C. After 30 minute incubation, Agglutinability of various RBC surface antigen (ABO, Rh-C, c, D, E, e) was measured by titration using anti-sera (Green Cross, Korea, Dade, USA), and compared the agglutinability changes before and after glutaraldehyde crosslinking. RESLUTS: The agglutinability of Rh surface antigens (D, C, c, E, e) was disappeared after glutaraldehyde crosslinking. However, ABO antigens (n=20) still showed strong agglutinability against antisera with some decreased. CONCLUSIONS: It would be useful to apply glutaraldehyde crossliked RBCs for rare blood group transfusion practice, if the safety problem were solved.
Antigens, Surface*
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Blood Substitutes
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Glutaral*
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Immune Sera
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Korea
;
Volunteers
6.Vibrio vulnificus serovars: a vailability for rapid identification method of vibrio vulnificus and prognostic value.
Korean Journal of Dermatology 1992;30(6):787-793
Vibrio vulnificus is a halophilic gram-negative, marine bacterium which causes fulminating and potentially fatal human disease. A new sensitive and specific serogrouping procedure was developed for identifying V. vulnificus. Reference antis:ra(01-014) to V. vulnificus were prepared by vaccinated rabbits with heat-killed bacteria, and the antisera were examined whether erogrouping test will be available for rapid and sensitive identification method of V. vulnifiius or not. All of thirty-seven clinical strains tested were agglutinated with 5 kinds of aritisera such as 04A, 01, 013, 03, and 014. Of these serovar 04A was predominant(75.7%). The author investigated the correlation among certain serovars, consumed seafoods, and nortality rate to verify whether the serovar is of pognostic value. There was no significant orrelation among them. These results suggest theart serogrouping procedure could be of value in rapidly identifying V. vulnificus.
Bacteria
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Humans
;
Immune Sera
;
Rabbits
;
Seafood
;
Vibrio vulnificus*
;
Vibrio*
7.Epitope Mapping of HIV1 gp41 Protein for Korean Anti - HIV1 Antisera using Synthetic Peptides.
Kyung Soo HAHM ; Myung Kyu LEE ; Song Yub SHIN ; Sun Young KIM ; So Youn JANG
Korean Journal of Immunology 1997;19(1):107-112
The N-terminal sequence of HIV1 gp41 (amino acid residues 584-623) was known to be the immundominant region of HIV1 gp41 protein. In order to determine epitope for gp41 protein of Korean anti-HIV1 positive sera, multiple antigenic peptides (MAPs) for the sequences corresponding to 584-604, 590-612, 604-623 and 584-618 of HIV1 gp41 were synthesized by solid phase method using Fmoc-Lys (Fmoc)-OH and used as coating antigens for ELISA. The reactivities of the synthetic peptides with Korean HIV1 positive (21 samples) and anti-HIV1 negative sera (22 samples) obtained from healthy blood doner were estimated by an indirect ELISA. MAPs for 584-604, 590-612 and 604-623 of gp41 reacted with 62 %, 100 % and 81 % of Korean anti-HIV1 positive sera tested, respectively. The results suggest that the epitope for HIV1 gp 41 for Korean anti-HIV1 positive sera is located in the region of amino acid 590-612 of gp41. MAP for gp41 (584-618) reacted with all (100 %) of anti-HIV1 positive sera tested, but did not react with anti-HlV1 negative sera. In addition, this MAP reacted stronger with seven samples of anti-HIV1 positive sera of anti-HIV1/2 combo performance panel than the mixture of 584-604, 590-612 and 604-623 of gp41, but did not react with anti-HIV negative serum. The high sensitivity and selectivity of MAP of gp41 (584-618) suggest that this peptide as a coating antigen in an ELISA system will be useful for antibody detection of HIV1.
Enzyme-Linked Immunosorbent Assay
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Epitope Mapping*
;
Immune Sera*
;
Peptides*
8.Reactivities of Commercial Antisera for HLA Typing.
Dong Il WON ; Jae Woo SONG ; Ki Il PARK ; Hyon Uk KIM
Korean Journal of Clinical Pathology 2000;20(5):510-515
BACKGROUND: The most common problem in HLA typing is unsatisfactory quality of the antisera, or a lack of understanding of their reactivities. Therefore, commercial antisera must be verified under the conditions applied in a particular tissue typing laboratory. METHODS: We evaluated the antisera reactivities of a commercial HLA-yping tray, Lymphotype HLA-BC 72 oriental, the lot 7220999, 7230100 (Biotest, Germany), in about 300 samples from organ transplant recipients and healthy potential donors. RESULTS: The relatively weak antisera were those that defined A26, A33, Cw5, Cw14, B46, B58, B64 and B71 etc. Some of these antisera were not indicated as 'weak reaction' in the test result catalogue. The reactivities of each antisera indicated as 'extra reaction' or 'sometimes missing' were various. CONCLUSIONS: As for antisera reactivities, the data obtained by a laboratory itself are necessary in addition to those in the test result catalogue. These data will be helpful for the correct interpretation for laboratories using same commercial kits.
Histocompatibility Testing*
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Humans
;
Immune Sera*
;
Tissue Donors
;
Transplants
9.Reactivities of Commercial Antisera for HLA Typing.
Dong Il WON ; Jae Woo SONG ; Ki Il PARK ; Hyon Uk KIM
Korean Journal of Clinical Pathology 2000;20(5):510-515
BACKGROUND: The most common problem in HLA typing is unsatisfactory quality of the antisera, or a lack of understanding of their reactivities. Therefore, commercial antisera must be verified under the conditions applied in a particular tissue typing laboratory. METHODS: We evaluated the antisera reactivities of a commercial HLA-yping tray, Lymphotype HLA-BC 72 oriental, the lot 7220999, 7230100 (Biotest, Germany), in about 300 samples from organ transplant recipients and healthy potential donors. RESULTS: The relatively weak antisera were those that defined A26, A33, Cw5, Cw14, B46, B58, B64 and B71 etc. Some of these antisera were not indicated as 'weak reaction' in the test result catalogue. The reactivities of each antisera indicated as 'extra reaction' or 'sometimes missing' were various. CONCLUSIONS: As for antisera reactivities, the data obtained by a laboratory itself are necessary in addition to those in the test result catalogue. These data will be helpful for the correct interpretation for laboratories using same commercial kits.
Histocompatibility Testing*
;
Humans
;
Immune Sera*
;
Tissue Donors
;
Transplants
10.Immunohistochemical Localization of Adenohypophyseal Gonadotropes in Korean Native Goat , Capra hircus.
In Se LEE ; Heung Sik LEE ; Moo Ho WON ; Je Hoon SEO ; Tae Chun KANG
Korean Journal of Anatomy 1997;30(6):649-657
The localizations and morphological characteristics of gonadotropes in the adenohypophy-sis of Korean native goat were investigated with double immunohistochemistry. The gonadotropes were present in the pars distalis and pars tuberalis, but not in the pars intermedta. Gonadotropes occupied about 49.0% of the cells in the pars distalis in females, and about 40.8% in males. Three types of gonadotropes ; FSH immunoreactive cells[FSH cells], LH immunoreac-tive cells[LH cells], and FSH and LH immunoreactive cells[FSH/LH cell], were identified according to their immunoreactivities for FSH and LH antisera. The possessional perce-ntages of FSH cells, LH cells and FSH/LH cells were 1.1%, 40.6%, 58.3% in females and 1.8%, 30.0%, 68.8% in males, respectively. FSH/LH cells were large and oval or round in shape. These cells were distributed throughout the pars distalis, but were more abundant on the dorsal part adjacent to the hypophyseal cavity and along the lateral and ventral peripheral regions. LH cells were smaller than other gonadotropes and were observed throughout the pars distalis, but predominant in the central region. FSH cells were large and oval in shape. These cells were intercalated between FSH/LH cells.
Female
;
Goats*
;
Gonadotrophs
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Humans
;
Immune Sera
;
Immunohistochemistry
;
Male