The objective of this study was to investigate the change of native immune adhering function (ENIAF) in self-plasma of patients with hematologic and lymphoid neoplasms and its effect on the killing activity of NK cells. The whole blood was anticoagulated with citric acid. 5 microl precipitated red blood cells and 500 microl plasma of patients or controls were directly mixed with 750 microl quantitative K562 cells at 37 degrees C for 30 minutes. One K562 cell attached by one or more erythrocytes was counted as one rosette, the ratio of rosettes was calculated. Using K562 cells as target cells, the killing activity of NK cells isolated from normal persons was detected by MTT assay, the change of the killing activity was observed after adding RBCs. The results indicated that the ratio of rosettes formed by RBCs of 21 normal controls and K562 cells was 15.3% +/- 6.4%, and the ratio of rosettes formed by RBCs of 24 patients and K562 cells was 7.6% +/- 7.0%. The ability of ENIAF in patients with hematologic and lymphoid neoplasms was significantly lower than that in healthy individuals (t = 3.61, p < 0.001). The killing rate of NK cells in peripheral blood of normal individuals was 67% - 71% without adding RBCs, and it increased by 14.7% +/- 5.2% after adding RBCs of normal controls but decreased by 4.3% +/- 7.6% with RBCs of patients. It is concluded that the ENIAF of RBCs in patients with hematopoietic and lymphoid neoplasms decreases, accompanying the reduction of the killing activity of NK cells to K562 cells, so to detect change of ENIAF may be helpful for the assessment of the immunological function of patients with hematopoietic and lymphoid neoplasms.
Adolescent ; Adult ; Erythrocytes ; immunology ; Hematologic Neoplasms ; immunology ; Humans ; Immune Adherence Reaction ; K562 Cells ; Killer Cells, Natural ; immunology ; Lymphoma ; immunology ; Middle Aged ; Rosette Formation ; Young Adult

Antitubercular Agents ; pharmacology ; therapeutic use ; Diagnostic Techniques, Respiratory System ; Drug Approval ; Drug Resistance ; Female ; Gene Amplification ; Humans ; Immune Adherence Reaction ; Male ; Treatment Failure ; Tuberculosis ; diagnosis ; drug therapy ; genetics

OBJECTIVETo investigate the effect of Shenmai Injection (SMI) on immunologic function in patients with dilated cardiomyopathy (DCM).

METHODSFifty-six patients were divided into two groups, the control group treated with conventional western medicine, and the SMI group treated with conventional western medicine plus SMI. The indices including red blood cell (RBC) C3b receptor rosette (RBC-C3bRR) and immune complex rosette (RBC-ICR), T-lymphocyte subsets (CD3, CD4, CD8, CD4/CD8) were determined before and after treatment.

RESULTSThe level of RBC-C3bRR, CD4, CD8 and CD3 in patients with DCM were significantly decreased (P <0 .01, P < 0.05), RBC-ICR and CD4/CD8 were significantly increased than those in the normal control group (P < 0.01); While the level of RBC-C3bRR, CD4, CD8 and CD3 in the SMI group after treatment were significantly higher, and the level of RBC-ICR and CD4/CD8 were significantly lower (P < 0.01, P < 0.05) than those in the control group.

CONCLUSIONThe RBC immune adherence and cellular immune function are lower in patients with DCM, and SMI has the effect in regulating immune function in patients with DCM.

Cardiomyopathy, Dilated ; drug therapy ; immunology ; Drug Combinations ; Drugs, Chinese Herbal ; therapeutic use ; Erythrocytes ; immunology ; Female ; Humans ; Immune Adherence Reaction ; Male ; Phytotherapy ; Plant Extracts ; therapeutic use ; Receptors, Complement 3b ; blood ; Rosette Formation ; T-Lymphocyte Subsets ; immunology