The author had investigated the effects of Na-K-ATPase activity on the incubation period, ATP and enzyme concentration, and various electrolytes in brain homogenate of Korean native goat. The results were summarized as follows: 1) The initial velocity of Na-K-ATPase reaction was in proportion to incubation period and enzyme concentration. However, the optimal reaction was found at 2 mM ATP, 50-100 mM Na, 5-10 mM K, 2-4 mM Mg, and pH 7.4 in medium. 2) Na concentration for the optimal activity of Na-K-ATPase was varied by K concentration and the ratio of K to Na concentration for optimal activity was roughly 1 to 5. 3) The Na-K-ATPase activity was inhibited by Ca. The inhibitory effect by Ca was an apparently mixed type with respect to Na activation of the Na-K-ATPase, by reducing apparent Vmax but increasing the apparent Km for Na. 4) The Na-K-ATPase activity was inhibited by cadmium and ouabain, The Km for cadmium and ouabain was 8+/-10 M and 1+/-10 M, respectively. The general characteristics of Na-K-ATPase activity in goat cerebral cortex were similar to that of another animals.
Adenosine Triphosphate ; Animals ; Brain ; Cadmium ; Cerebral Cortex* ; Electrolytes ; Goats* ; Hydrogen-Ion Concentration ; Ouabain