The authors studied the ultrastructural changes of liver cell mitochondria in autolysis. The male Sprague-Dawley rats, weighting 140~160 gm were sacrificed for extract liver tissue. The slices of the liver tissue were incubated in 37 degrees C aseptic normal saline, and those were examined by following intervals; 10, 20 and 30 minutes and 1, 2, 6, 12 and 24 hours with light and electron microscope. The results obtained by light microscopy were summerized as follow. Several fine intracytoplasmic vacuoles were observed 1 hour after incubation. After 3 hours, focal destruction of cytoplasmic membrane with pyknosis of nuclei were observed. More delicate intracytoplasmic architectural changes could not be detected at light microscopic studies. The cord arrangement and cellular boundaries were relatively well preserved until 24 hours after incubation. Electronmicroscopically, mild intramitochondrial swelling with diminution of intramatrical granules were observed at 20 minutes. These were the earliest findings. Both high amplitude swelling and destruction of mitochondrial membrane were observed concurrently at 1 hour after incubation. The earlier membrane changes were observed at inner membrane with cristae and followed by degeneration of the outer membrane. The intramatrical amorphous dense deposits were observed at 30 minutes when the membranes were not destroyed. These deposits were noted in the other experimental groups which were incubated longer than 30 minutes. More electron dense deposits were observed after 1 hour at that time the membrane changes appeared. Vhe results suggest in this experiment that the earliest autolytic changes of liver cell mitochondria is loss of intramitochondrial granules and the membraneous changes led to the irreversible mitochondiral injury. The appearance of two types of intramitochondrial dense deposits would be an interesting finding needed to require further investigation for the chemical stucture and mechanism of dense deposit formation.
Male ; Humans

No abstract available.

The authors studied the formation of giant mitochondria in liver cell. The Sprague Dawley rats were sacrificed following intervals; 5, 10, 20, 30, and 60 minutes after intraperitoneal injection of hydrazine in the amount of 200 microliter/kg. And the extracted liver tissues were examined with light and electron microscopes. The results obtained were summarized as follow; Light microscopically, there is little difference between control and experimental groups. Electron microscopically, elongated, bizzare shaped mitochondria are appears 5 minutes after hydrazine injection. Those show attenuated portion, Y, U, or C shaped feature suggesting fusion or budding mitochondria. The number of giant mitochondria is decreased after 10 minutes group and rarely present in 60 minutes group. The results suggest in this experiment that the formation of giant mitochondria is kind of reversible change and it is different from the mitochondrial swelling of cellular injury. Intermitochondrial fusion and mitochondrial budding may be related with the formation of giant mitochondria.
Rats ; Animals

No abstract available.
Germ Cells* ; Neoplasms, Germ Cell and Embryonal*

No abstract available.
Case-Control Studies* ; Female* ; Humans ; Lung Neoplasms* ; Lung* ; Smoke* ; Smoking*

No abstract available.
Krukenberg Tumor*

Upper canine is described as 'cornerstone' of maxilla, and its importance is implicated by long root and good alveolar support, The incidence of impaction of upper canine is the second most frequent next to the third molar because it takes a long period of time to develop, and has a complicated path of eruption, and erupts lately. Generally, the patient who has a palatally impacted canine visit the clinic primarily due to a missing of canine after 12-13 years old. Palatally impacted canine is different from labially impacted canine in its cause and treatment process, It is due to malposition or anomalous lateral incisor rather than arch length deficiency. Once the impaction is identified, the first stage of the treatment is to localize the lesion by radiographic examinations or others and according to severity, orthodontic traction, or transplantation should be considered, and comprehensive diagnosis and treatment plan of malocclusion should be established, Properly managed impacted canine can provide funtion and esthetic through proper diagnosis and treatment of extraction of canine is not indicated.
Diagnosis ; Humans ; Incidence ; Incisor ; Malocclusion ; Maxilla ; Molar, Third ; Traction

The authors report a rare case of thalamic abscess in a 38-year old male who suffered from a ventricular septal defect. Preoperative axial magnetic resonance imaging(MRI) showed two ring enhancing masses on T1-weighted image and a hypointensity of dark rim on T2-weighted image. Purulent material was aspirated by computerized tomography(CT)-guided stereotactic procedure following antibiotic therapy for 3 days. No growth was obtained in the culture of the purulent material. The disturbance of left ocular movement before surgery was completely recovered. Even though the abscess wall is uniform in thickness, it is difficult to distinguish from tumor. A rim like hypointensity surrounding the mass on T2-weighted MRI is helpful in distinguishing both lesions.
Abscess* ; Adult ; Heart Septal Defects, Ventricular ; Humans ; Magnetic Resonance Imaging ; Male

The authors report a rare case of thalamic abscess in a 38-year old male who suffered from a ventricular septal defect. Preoperative axial magnetic resonance imaging(MRI) showed two ring enhancing masses on T1-weighted image and a hypointensity of dark rim on T2-weighted image. Purulent material was aspirated by computerized tomography(CT)-guided stereotactic procedure following antibiotic therapy for 3 days. No growth was obtained in the culture of the purulent material. The disturbance of left ocular movement before surgery was completely recovered. Even though the abscess wall is uniform in thickness, it is difficult to distinguish from tumor. A rim like hypointensity surrounding the mass on T2-weighted MRI is helpful in distinguishing both lesions.
Abscess* ; Adult ; Heart Septal Defects, Ventricular ; Humans ; Magnetic Resonance Imaging ; Male

The origin and clinical importance of subdural hygroma(SDG) are still uncertain. Its pathogenetic mechanism and natural history have not yet been settled. Although the incidence of traumatic SDG has been reported to be 5-20% of posttraumatic space-occupying lesions, the true incidence has not been documented in prospective study. Therefore, authors have tried to determine the incidence of traumatic SDG prospectively during past six months. Serial computed tomography(CT) or magnetic resonance imaging(MRI) studies were done in all patients who were admitted to our department after head injuries. Data on the age, sex, Glasgow coma scale(GCS) on admission, and initial CT findings were collected and analyzed to determine the true incidence, pattern and premorbid conditions for the development of traumatic SDG. Serial CT or MRI scans were performed on the date of admission, the second to sixth hospital day, and the seventh to fourteenth hospital day. Study population consisted of 115 patients, excluding 31 expired, discharged, or transferred patients within a week. Subdural hygroma was noted in 42(35.6%) patients. It shared 45.2% of posttraumatic mass lesion. More than half (54.7%) of patients aged 40 or more had subdural hygromas. They were generally delayed lesions, due to the fact that most of them(81%) were observed at four days or more after the injury. All hygromas were located at the frontal or fronto-temporo-parietal regions. Bilaterality was seen in 54.7%. SDGs occurred earlier when the age of the patients were 40 years old or more(p=0.037). It occurred earlier when the initial CT scans were normal, when there was no accompanying traumatic intracranial lesions, and high GCS on admission. However, these differences were statistically not significant(p>0.05). These results suggest that the premorbid conditions for the development of subdural hygroma were sufficient potential subdural space and separation of the dural border cell layer, although former seemed to be more important that the later. Osmotic dehydration in the aged victims should be serially reevaluated, because the subdural hygroma may develop when the intracranial pressure is excessively low.
Adult ; Coma ; Craniocerebral Trauma ; Dehydration ; Epidemiology ; Humans ; Incidence* ; Intracranial Pressure ; Lymphangioma, Cystic ; Magnetic Resonance Imaging ; Natural History ; Prospective Studies* ; Subdural Effusion* ; Subdural Space ; Tomography, X-Ray Computed