No abstract available.
Foot* ; Free Tissue Flaps*

This study was performed to investigate the stimulating effect on oocyte maturation and cumulus cell expansion in TC199 media by human cord serum (HCS) supplementation. Immature mouse oocyte cumulus complexes (OCCs) were cultured in TC199 media supplemented with bovine serum albumin (BSA), HCS and human chorionic gonadotropin (hCG) instead of luteinizing hormone (LH) respectively, and the expression of cumulus expansion and oocyte maturation were observed. After 4hr and 24hr culture with or without OCCs, media containing 0.4% BSA, 10% HCS and 10 lU hCG respectively were collected and analyzed for changing concentrations of estradiol (E2), progesterone(P4), testosterone(T), and PGF2. There were no elevation of E2, T, and PGF2 by OCCs culture, but minute elevation of P4 level by 24hr OCCs culture in hCG supplementation (p=0.048). The stimulating pattern of cumulus expansion of OCCs by HCS and hCG supplementation was similar to our previously report using Ham's F-10 media, however oocyte maturation rates after 24hr OCCs culture in all media were increased by 20~30% compared to Ham's F-10 media. These results suggest that LH in HCS induce cumulus expansion probably by P4 secretion of OCCs, and TC199 is efficient media for immature mouse oocyte maturation.
Animals ; Chorionic Gonadotropin ; Cumulus Cells* ; Dinoprost ; Estradiol ; Humans* ; Luteinizing Hormone ; Mice ; Oocytes* ; Serum Albumin, Bovine

No abstract available.
Thumb*

INTRODUCTION: It has been proposed that narcolepsy and REM sleep behavior disorder (RBD) have overlapped symptom profile and pathophysiology. This study was aimed at measuring and comparing changes in EEG frequency band of REM sleep in narcolepsy and RBD, applying EEG spectral analysis method. METHODS: Nine patients diagnosed as narcolepsy and the same number of RBD patients were studied. Spectral analysis of the REM sleep EEG was performed in each patient on 9 epochs selected evenly from the first, second, and third REM periods. Then, we compared frequency band percentages of REM sleep EEG in narcolepsy and RBD. RESULTS: Narcolepsy patients had significantly higher delta frequency ratio than RBD ones (p=0.00). In alpha and beta2 frequency bands, RBD patients showed higher percentage than narcolepsy ones. Slow wave sleep was more prevalent in narcolepsy patients. But, no difference of REM sleep percentage was found between the two groups (p=0.93). CONCLUSION: Higher delta frequency ratio in REM sleep of narcolepsy patients than RBD ones reflects that sleep-promoting mechanism is more dominant in narcolepsy than in RBD.
Electroencephalography ; Humans ; Narcolepsy ; REM Sleep Behavior Disorder ; Sleep, REM

In order to elucidate the possibility of diagnosis in soft tissue cysticercosis in case no parasite in it, immunohistochemical staining with antihuman IgG, IgM, and IgA and anticysticercus antibody in tissue section were carried out. Not only the differential counts of infiltrated plasma cells but also the parasite itself against to the anticysticercus antibody were observed. Materials stained immunohistochemically were consisted of 21 soft tissue cysticercosis with or without parasite. The results were as follows: 1) IgG producing plasma cells were most frequent (45.8+/-18.7) and followed by IgM (14.3+/-0.43) and (4.3+/-5.71). Number of IgG plasma cells were more frequently accompanied in the presence of cysticercus (55.9+/-17.7) than in the absence of parasite (39.7+/-17.1). 2) The mean+/-S.D. of plasma cells positive to the anticysticercus antibody were 28.2+/-18.7. And anticysticercus antibody producing plasma cells were more frequent in the presence of cysticercus (49.2+/-5.16) than in the absence of parasite (15.2+/-9.46). 3) Antigenicity of the cysticercus was strong in inner layer(#) followed by tegument(+) and fibrous layer(-). 4) Therefore, it could be suggested that antibody against to the cysticercus is mainly produced by IgG plasma cells and antigenicity of the worm is strong in the inner layer of cyst wall. In addition, there is a possibility of assisting the diagnosis of cysticercosis with anticysticercus antibody by immunohistochemically.
Cysts

No abstract available in English.
Torticollis

Zielke introduced new anterior instrumentation by modifying Dwyer system and called it the ventral derotation spondylodesis(VDS) system in 1976. This system is a powerful device and when the excised disc spaces are compressed with it, stable fixation can be obtained. But proper patient selection, meticulous operative technique and good post-operative care are essential to minimize serious complications. No results of this technique had been published in English-language literature until Moe reported the results of 66 cases of Zielke instrumentation in 1983. Zielke instrumentation was carried out in 12 cases of scoliosis;8 in idiopathic scoliosis and 4 in congenital scoliosis, at the Department of Orthopedic Surgery, Seoul National University Hospital, for 16 months from November 1984 to March 1986. Seven cases had been followed for more then 9 months and'following results were obtained. 1. Numbers of vertebrae involved in major curve and numbers of fused vertebrae were 6·3 respectively. 2. Average preoperative curve was 72.7 degrees and immediate postoperative curve was 17.6 degrees with 75.8% correction. There was only 1.0 degree loss of correction with an average follow-up of 14 months (9-16 months). 3. Zielke instrumentation has the advantage of shorter fusion and better correction in thoracolumbar and lumbar scoliosis.
Follow-Up Studies ; Orthopedics ; Patient Selection ; Scoliosis ; Seoul ; Spine

No abstract available.
Cerebral Palsy* ; Early Diagnosis* ; Neurologic Examination*

No abstract available.
Leg* ; Skin*

No abstract available.