Since the culture of M. furfur is impossible, the KOH wet mount and various staining techniques have been applied for identification of the M. furfur. However, these methods still have many disputed points. Practically, the KOH wet mount method is in common use but. there are many difficulties in identifying the fungi. The author intended to suggest an easy and simple method for identification of the fungi, using the KOH and various other staining solutions, and comparing this with many known methods. At the same time, by applying the best method of identification which the author was able to develop, distribution of the fungi in the horny layer and the viability of the fungi during treatment were abserved In identifying the fungi, 1% toluidine blue was most excellent, but hematoxylin, eosin, cotton blue, Giemsa stain, and Wright stain were not so satisfactory. 2. After staining with l% toluidine blue to the skin lesion scotch tape was applied to the lesion briefly and then examined under direct microscope. This was most easy and convenient method. 3. Repeated scotch taping from ] to 12 times produced no change in the distribution of fungi in the horny layer, but after 28 applications there was remarkable reduction of the amount of the fungi and no fungi was demonstrated in groups taped more than 46 times. 4. No influence was noted in the distribution of fungi after repeated irradiation of Ultra-violet light once daily for 18 days. 5. Continous daily application of 25% sodium thiosulfate solution for average 3. 9 days, caused the disappearance of tungi and no fungal elements were found following EO days of successive observation. 6. Application of 2.5% selenium sulfide on alternate day for average 6.2 days, caused the disappearance of fungi and no fungal elements were found following 55- 62 days of successive observation.
Azure Stains ; Eosine Yellowish-(YS) ; Fungi ; Hematoxylin ; Malassezia* ; Selenium ; Skin ; Sodium ; Tinea Versicolor* ; Tinea* ; Tolonium Chloride

Two cases of Klippel-Trenaunay-Weber Syndrome were presented and its literatures were reviewed. The patients were 25 year old male farmer and 38 year old housewife. Both had hypertrophy, port-wine nevus, and varicosities in case 1, on left lower extremity since birth. The length and cirumference of the affected leg were longer than the other side, and X-ray showed osteohypertrophy of tibia of the involved leg,
Adult ; Humans ; Hypertrophy ; Klippel-Trenaunay-Weber Syndrome* ; Leg ; Lower Extremity ; Male ; Nevus ; Parturition ; Tibia

Two cases of Klippel-Trenaunay-Weber Syndrome were presented and its literatures were reviewed. The patients were 25 year old male farmer and 38 year old housewife. Both had hypertrophy, port-wine nevus, and varicosities in case 1, on left lower extremity since birth. The length and cirumference of the affected leg were longer than the other side, and X-ray showed osteohypertrophy of tibia of the involved leg,
Adult ; Humans ; Hypertrophy ; Klippel-Trenaunay-Weber Syndrome* ; Leg ; Lower Extremity ; Male ; Nevus ; Parturition ; Tibia

Tow cases of congenital ectodermal defect in brothers aged 15 and 10 years were pesinted and the literature was reviewed. They had lack of sweating, sparseness of the hard hairs, absence of lanugo hairs, maldevelopment or defect of most of the teeth, etc. They seemed to be angidrotic type associated with a sex-linked reccessive inheritance in their family backgroud.
Ectodermal Dysplasia* ; Hair ; Humans ; Siblings ; Sweat ; Sweating ; Tooth ; Wills

We describe a riboprinting scheme for identification of unknown Acanthamoeba isolates at the species level. It involves use of the PCR-RFLP of small subunit ribosomal RNA gene (riboprint) of 24 reference strains by 4 kinds of restriction enzymes. Seven strains in morphological group I and III were identified at species level with their unique sizes of PCR product and riboprint type by Rsa I. Unique restriction fragment length patterns of 17 strains in group II by Dde I, Taq I and Hae III were classified into: (1) four taxa that were identifiable to the species level, (2) a subgroup of 4 taxa and a pair of 2 taxa that were identical to each other, and (3) a species complex of 7 taxa assigned to A. castellanii complex that were closely related. These results were consistent with that of 18s rDNA sequence analysis. This approach provides an alternative to the rDNA sequencing for rapid identification of a new clinical isolate or large number of environmental isolates of Acanthamoeba.
Acanthamoeba/classification/genetics/*isolation & purification ; Animals ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; RNA, Protozoan ; RNA, Ribosomal ; Ribotyping/*methods

No abstract available.
Hyperaldosteronism*

The cyclophilins (Cyps) are family members of proteins that exhibit peptidylprolyl cis-trans isomerase (PPIase, EC 5.2.1.8) activity and bind the immunosuppressive agent cyclosprin A (CsA) in varying degrees. During the process of random sequencing of a cDNA library made from Giardia intestinalis WB strain, the cyclophilin gene (gicyp 1) was isolated. An open reading frame of gicyp 1 gene was 576 nucleotides, which corresponded to a translation product of 176 amino acids (Gicyp 1). The identity with other Cyps was about 58-71%. The 13 residues that constituted the CsA binding site of human cyclophilin were also detected in the amino acid sequence of Gicyp 1, including tryptophan residue essential for the drug binding. The single copy of the gicyp 1 gene was detected in the G. intestinalis chromosome by southern hybridization analysis. Recombinant Gicyp 1 protein clearly accelerated the rate of cis-- Amino Acid Sequence ; Animals ; Cloning, Molecular ; Cyclophilins/antagonists & inhibitors/chemistry/genetics/*isolation&purification ; Cyclosporine/metabolism/pharmacology ; Giardia lamblia/*chemistry/genetics ; Human ; Immunosuppressive Agents ; Molecular Sequence Data ; Protein Binding ; Protozoan Proteins ; Recombinant Proteins

Variation in the facial pattern and effect of the rotational jaw growth on the facial proportion were studied in serial cephalometric radiographs of 40 Korean children (35 boys, 15 girls) ranging in age from 6 to 13 years. According to Y-axis (N-S-Gn) growth change during the 7 years of period, the subject who had exhibited "wave-like" manner of Y-axis growth change within +/- 2degrees was classified to the Parallelwise group and the subject who had exhibited Y-axis increase more than +/- 2degrees was classified to the Clockwise rotation group and the subject who had exhibited Y-axis decrease more than -2degrees was classified to the Counterlockwise rotation group. For the comparison of each group, a total of 22 morphologic variables were employed and the data were analyzed by means of computer morphometrics and statistical methods. On the basis of the finding of this study, the following trends were eatablished: 1. The Parallelwise group was 75%, the Clockwise rotation group was 12.5% and the Counterclockwise rotation group was 12.5%. 2. The growth pattern of cranial base was related to the rotation of mandible. 3. Maxillary prognathism was occured in the Counterclockwise rotation group and the rotation of palatal plane was occured in the Clockwise rotation group. 4. Mandibular prognathism was occured in the Parallelwise and the Counterclockwise rotation groups, especially in the Counterclockwise rotation group. 5. The degree of maxillo-mandibular divergency was constant in the Clockwise rotation group but decreased in the Counterclockwise rotation and the Parallelwise groups, especially in the Counterclockwise group. 6. There were no differences in the size of the anterior upper facial height (N-ANS) and the posterior lower facial height ((Go-Me)-PNS) but there were differences in the size of the anterior lower facial height (ANS-Me) and the posterior upper facial height ((S-N)-PNS) between each group. 7. The growth increment and the size of the facial gepth was not relater to the growth pattern of the face but the growth increment and the size of the facial height was related to the growth pattern of the face. 8. Proportional change due to the facial growth rotation was concentrated in the anterior lower face. 9. The most apparent difference between each group was happened in the size of the posterior cranial base (S-Ba) and the facial length (S-Gn).
Child ; Humans ; Jaw ; Mandible ; Prognathism ; Skull Base

The congenital dislocation of the hip is one of the most common congenital disease of the infants. The diagnosis and treatment of comgemital dislocation of the hip has been developcd since Lorenz published the methods of the closed reduction and immobilization in 1895. By replacing the displaced femoral head in the acetabular socket in early time, the normal development of acetabulum and femoral head were expected. So the early diagnosis and treatment were very important for good results. The authors studied 19 cases of 18 patients of congenital dislocation of the hip at the Department of Orthopedic Surgery of Hanyang University Hospital from June, 1972 to June, 1977. As results of this study, the following concolusion were result reached: 1. The proponderance of girl to boy was 13: 5. 2. The ratio of left side to right was 12:7. 3. The chief complaints were shortening of lower extremity, limitation of hip motion(esp. abduction) below 1 year old age and limping in over 1 year old age. 4. The associated congenital anomaly was abserved in a case of internal tibial torsion and metatarsus varus deformity. 5. In birth history, there was 1 case of breech presentation. 6. The acetabular index was much decreased in involved hip than the sound hip in treatment. 7. The arthrogram was valuable to detect any obstacles of closed reduction. 8. In 16 cases of 15 patients, 13 cases were treated by closed reduction, 1 case was open reduction, 1 case was derotation osteotomy, 1 case was varus and derotation osteotomy. 9. The results of treatment was Excellent:8 Good:8.
Acetabulum ; Breech Presentation ; Clinical Study ; Congenital Abnormalities ; Diagnosis ; Dislocations ; Early Diagnosis ; Female ; Head ; Hip ; Humans ; Immobilization ; Infant ; Lower Extremity ; Male ; Metatarsus ; Orthopedics ; Osteotomy ; Pregnancy ; Reproductive History

CT and myelography, separately or combined together, have been used for the study of spine lesion. Metrizamide enhanced CT is a new diagnostic modality for the study of spine lesion. The purpose of this study was to compare the efficacy of metrizamide enhanced CT with those of CT and metrizamide myelography. Among 163 surgically proven cases, metrizamide CT was carried out on 49 cases, CT on 50 cases and myelography on 64 cases, at Department of Orthopedic Surgery, Seoul University Hospital for 3 years period from August 1981 to July 1984. 1. Overall diagnostic accuracy of CT was 90%, that of metrizamide myelography was 82.8%and that of metrizamide CT was 93. 9%. 2. Diagnostic accuracy of metrizamide CT in HIVD was 95%, in spinal stenosis 94.4% and in spondylolisthesis 80%. 3. Most of complications were observed in myelography, even they were minor. 4. It was concluded that plain CT was advisable on a clinically typical HIVD, considering complications and expenses of myelography and metrizamide CT. 5. Metrizamide CT was indicated on previously operated cases or atypical HIVD, spinal stenosis and spondylolisthesis.
Metrizamide ; Myelography ; Orthopedics ; Seoul ; Spinal Stenosis ; Spine ; Spondylolisthesis