1.Mitochondria-Targeted Vitamin E Protects Skin from UVB-Irradiation.
Won Serk KIM ; Ikyon KIM ; Wang Kyun KIM ; Ju Yeon CHOI ; Doo Yeong KIM ; Sung Guk MOON ; Hyung Keun MIN ; Min Kyu SONG ; Jong Hyuk SUNG
Biomolecules & Therapeutics 2016;24(3):305-311
Mitochondria-targeted vitamin E (MVE) is designed to accumulate within mitochondria and is applied to decrease mitochondrial oxidative damage. However, the protective effects of MVE in skin cells have not been identified. We investigated the protective effect of MVE against UVB in dermal fibroblasts and immortalized human keratinocyte cell line (HaCaT). In addition, we studied the wound-healing effect of MVE in animal models. We found that MVE increased the proliferation and survival of fibroblasts at low concentration (i.e., nM ranges). In addition, MVE increased collagen production and downregulated matrix metalloproteinase1. MVE also increased the proliferation and survival of HaCaT cells. UVB increased reactive oxygen species (ROS) production in fibroblasts and HaCaT cells, while MVE decreased ROS production at low concentration. In an animal experiment, MVE accelerated wound healing from laser-induced skin damage. These results collectively suggest that low dose MVE protects skin from UVB irradiation. Therefore, MVE can be developed as a cosmetic raw material.
Animal Experimentation
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Cell Line
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Collagen
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Fibroblasts
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Humans
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Keratinocytes
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Mitochondria
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Models, Animal
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Reactive Oxygen Species
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Skin*
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Vitamin E*
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Vitamins*
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Wound Healing
2.Development of S-Methylmethionine Sulfonium Derivatives and Their Skin-Protective Effect against Ultraviolet Exposure.
Won Serk KIM ; Wang Kyun KIM ; Nahyun CHOI ; Wonhee SUH ; Jinu LEE ; Dae Duk KIM ; Ikyon KIM ; Jong Hyuk SUNG
Biomolecules & Therapeutics 2018;26(3):306-312
In a previous study, we have demonstrated that S-methylmethionine sulfonium (SMMS) confers wound-healing and photoprotective effects on the skin, suggesting that SMMS can be used as a cosmetic raw material. However, it has an unpleasant odor. Therefore, in the present study, we synthesized odor-free SMMS derivatives by eliminating dimethyl sulfide, which is the cause of the unpleasant odor and identified two derivatives that exhibited skin-protective effects: one derivative comprised (2S,4S)- and (2R,4S)-2-phenylthiazolidine-4-carboxylic acid and the other comprised (2S,4R)-, (2S,4S)-, (2R,4R)-, and (2R,4S)-2-phenyl-1,3-thiazinane-4-carboxylic acid. We performed in vitro proliferation assays using human dermal fibroblasts (hDFs) and an immortalized human keratinocyte cell line (HaCaT). The two SMMS derivatives were shown to increase hDF and HaCaT cell proliferation as well as improve their survival by protecting against ultraviolet exposure. Moreover, the derivatives regulated the expression of collagen type I and MMP mRNAs against ultraviolet exposure in hDFs, suggesting that these derivatives can be developed as cosmetic raw materials.
Cell Line
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Cell Proliferation
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Collagen Type I
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Fibroblasts
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Humans
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In Vitro Techniques
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Keratinocytes
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Odors
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Reactive Oxygen Species
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RNA, Messenger
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Skin
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Vitamin U*