1.Bcr rearrangement analysis using digoxigenin-dUTP.
Hee Jung KANG ; Se Ik JOO ; Sung Sup PARK ; Han Ik CHO ; San In KIM
Korean Journal of Hematology 1991;26(2):299-305
No abstract available.
2.Chromosomal Abnormalities in Human Hepatoma.
Sung Ik CHUNG ; Sang Heun BAIK ; Hong Tage KIM
Korean Journal of Physical Anthropology 1995;8(2):185-193
To a better understanding for molecular mechanism of oncogenesis in hepatoma, primary hepatocellular carcinoma and hepatoma cell lines (Hep 3B, PLC/PRP/5, Hep G2) were subjected to detailed cytogenetic analysis with G-banding method after cell cultures. No cloned chromosomal abnormalities were found in the primary hepatoma (below 100%). On the other hand, all hepatoma cell lines were cloned, the specific chromosomal abnormalities in Hep 3B were del(1p21), del(6q14) and t(1 ; 11)(pll ; q13). Genes of AMY1A, CGA, SEA and HSTF1 were located on 1p21 and 6q14 respectively. SEA and HSTF1 were located on 11q13. Regions of chromosome abnormalities in PLC/PRF/5 were the same found in Hep 3B. Besides, del(1q32) and del(1p32) were also cloned. Gene of CR1 and MYCL1 were located on 1q32 and 1p32 respectively. The characteristic findings of chromosome abnormalities in Hep G2 were del(1p31) and del(1q22). And GST1 and DAF were located on these regions each other Del(6q11) and del(1p22) were also found in Hep G2. From the above results, it is presumed that HBV may integrate to AMY1A gene or near this gene and leads to loss of functions to this gene. And impaired regulation of CGA occurs in next step. SEA, HSTF1 and MYCL1 oncogenes may act as a progressing factor of tumourgenesis in HBsAg(+) hepatoma. Some factors like chemical agents may cause functional loss of GST1 and DAF at first and functional loss of cell regulation of CGA occurs in next step. SKI oncogene may promote the progression of carcinogenesis in this cell line. Whether any causative agents are involved in carcinogenesis of hepatoma, functional loss of CGA gene is the most important factor in tumour-genesis in hepatoma.
Carcinogenesis
;
Carcinoma, Hepatocellular*
;
Cell Culture Techniques
;
Cell Line
;
Chromosome Aberrations*
;
Clone Cells
;
Cytogenetic Analysis
;
Hand
;
Humans*
;
Methods
;
Oncogenes
3.Squamous Cell Carcinoma Arising from Chronic Osteomyelitis: A report of four Cases
Ik Soo CHOI ; Woo Il KIM ; Sung LIM
The Journal of the Korean Orthopaedic Association 1994;29(2):386-393
Squamous cell carcinoma from the draining sinus of chronic osteomylitis has been recognized as a rare complication. The incidence of this complication is between 0.23% and 1.6% in cases of osteomyelitis. The cause of development of carcinoma at the site of chronic osteomyelitis is not well understood. This is a disease of middle aged men, and tibia is the most common site. Biopsy of squamous cell carcinoma which arises in the proliferating edge of the cutaneous ulcer and invades the bone, should include tissues from all sites of ulcer and bone marrow spaces. Histological diagnosis may be difficult because of preexisting metaplasia and pseudoepitheliomatous hyperplasia. Amputation at the adequate level is the treatment of choice. Four cases of squamous cell carcinoma involving tibia, metatarsal bone or calcaneus are reported with review of literatures.
Amputation
;
Biopsy
;
Bone Marrow
;
Calcaneus
;
Carcinoma, Squamous Cell
;
Diagnosis
;
Epithelial Cells
;
Humans
;
Hyperplasia
;
Incidence
;
Male
;
Metaplasia
;
Metatarsal Bones
;
Middle Aged
;
Osteomyelitis
;
Tibia
;
Ulcer
4.Mechanism of oncogenesis and activation of oncogene in human ovarian carcinoma..
Young Wook SUH ; Tae Sung LEE ; Sung Ik CHANG ; Dae Kwang KIM ; Hong Tae KIM
Korean Journal of Physical Anthropology 1992;5(2):123-137
No abstract available.
Carcinogenesis*
;
Humans*
;
Oncogenes*
5.Different mechanism of oncogenesis by individuals in solid tumours..
Sung Ik CHANG ; Dae Kwang KIM ; Hong Tae KIM ; Tae Sung LEE
Korean Journal of Physical Anthropology 1992;5(2):111-122
No abstract available.
Carcinogenesis*
6.Correlation between Chromosome Abnormalities and Genomic Imprinting in Developing Human 2) Independent Expression of Imprinted Genes, H19 and Insulin-like growth factor II (IGF2), in Androgenetic Hydatidiform Moles.
Sung Ik CHANG ; Bo Hwa CHOI ; Tae Sung LEE ; Dae Kwang KIM ; In Hwan LEE
Korean Journal of Anatomy 1998;31(4):611-617
Human uniparental gestations such as androgenetic hydatidiform moles provide a model to evaluate the integrity of parent-specific gene expression,-i.e, genomic imprinting,- in the absence of a complementary parental genetic contribution. Several imprinted genes are characterized so far including the insulin-like growth factor-2 gene (IGF2) coding for a fetal growth factor and the Hl9 gene whose normal function is unknown but which is likely to act as an untranslated mRNA for its tumor-suppressing function. IGF2 is expressed exclusively from the paternal allele while Hl9 from the maternal allele. Such an alternate expression is quite interesting because both Hl9 and IGF2 genes are located close to each other on chromosome 11p15.5. An in situ hybridization analysis has shown strong expression of Hl9 and IGF2 alleles in nine hydatidiform moles. Especially, a prominent expression of Hl9 and IGF2 was detected in cytotrophoblast and the cellular localization was almost paralleled in Hl9 and IGF2 transcripts . Hl9 and IGF2 genes could be expressed either biallelically or monoallelically in the moles. However, IGF2 biallelic expression did not affect allele-specificity of Hl9 expression.. These results suggest that both H19 and IGF2 transcripts are expressed in the same cells and that the functional imprinting of H19 and IGF2 genes in hydatidiform moles can be controlled individually and independently of each other.
Alleles
;
Chromosome Aberrations*
;
Clinical Coding
;
Female
;
Fetal Development
;
Genomic Imprinting*
;
Humans*
;
Hydatidiform Mole*
;
In Situ Hybridization
;
Insulin-Like Growth Factor II*
;
Parents
;
Pregnancy
;
RNA, Messenger
;
Trophoblasts
7.Esophageal perforation during endotracheal intubation.
Sung Chul KIM ; Sang Ik KIM ; Sang Joon OH ; Chang Ho KIM
The Korean Journal of Thoracic and Cardiovascular Surgery 1992;25(11):1231-1235
No abstract available.
Esophageal Perforation*
;
Intubation, Intratracheal*
8.A case of Lipoleiomyoma of the Uterus.
Hea Su SHIN ; Sung Min SON ; Young Min YANG ; Tae Sang KIM ; Ik Su KIM
Korean Journal of Obstetrics and Gynecology 2000;43(10):1853-1856
No abstract available.
Uterus*
9.A Case of Sirenomelia.
Hyun Kuk KIM ; Sung Ik CHO ; Byoung Tae KIM ; Hak Jhoo CHA ; Sang Jhoo LEE
Journal of the Korean Pediatric Society 1990;33(2):241-245
No abstract available.
Ectromelia*
10.The Effect of Tretinoin Intradermal Injection on Dermal Thickening in Rabbit.
Ik Jun LEE ; Moo Hyun PAIK ; Seung Hong KIM ; Sung Taek KIM
Journal of the Korean Society of Plastic and Reconstructive Surgeons 2000;27(1):78-82
Tretinoin(all-trans retinoic acid) is a metabolite of vitamin A and it is useful in the treatment of photoaging skin. Photoaging skin is characterized by wrinkles, mottled pigmentation, dry and rough skin, and loss of skin tone. Current use of topical tretinoin mainly acts on the epidermis, requires a long period to obtain the desired results and may cause skin hyperpigmentation. A combination of topical and injectable tretinoin has been used to reduce the treatment period as a result of its potentialized effect on the dermis when compared to graditional topical cream use. in this study, we observed histologic alterations in 5 white rabbits after using 0.05% topical tretinoin cream and 0.1% injectable tretinoin. Tretinoin was treated on the rabbits ears-group 1 and 2 on the right ear for study 1, and group A and B on the left ear for study 2. Study 1 was done to differentiate whether the dermal thickening is due to the simple physical stretching of dermis by intradermal injection, or whether it is duer to the histologic change by tretinoin. In group 1, saline was injected intradermally and in group 2, tretinoin was injected intradermally. Study 2 was done to compare the dermal thickening between the topical tretinoin cream treatment group (group A) and the combined topical and injectable tretinoin group (group B). Injection was done once a week immediately followed by 340nm blue light skin exposure. These treatment were done for 12 weeks. We harvested skin stripe from all group, group 1 and 2, and group A and B respectively, after 2, 6, and 12 weeks after treatment. Histologic differences were observed and measured. Dermal thickening was observed in group 2 and in group B(p<0.05). The results showed that intradermal injection of tretinoin mainly acts on the dermis and potentialtes the effect on photo-aging skin and fine wrinkles.
Dermis
;
Ear
;
Epidermis
;
Hyperpigmentation
;
Injections, Intradermal*
;
Pigmentation
;
Rabbits
;
Skin
;
Tretinoin*
;
Vitamin A