Infectious bursal disease virus (IBDV) was inactivated by two different chemicals--formaldehyde and binary ethylenimine (BEI). Formaldehyde was used at 0.1% and 0.2%, while BEI was used at concentrations of 0.001 and 0.002 mol/L. These four vaccines were tested for their efficiency in generating humoral immune response in different groups of broiler chicks. Both BEI-inactivated vaccines gave relatively higher antibody titers and were almost twice as efficient as formaldehyde-inactivated ones.
Animals ; Antibodies, Viral ; blood ; Aziridines ; pharmacology ; Chickens ; Formaldehyde ; pharmacology ; Infectious bursal disease virus ; immunology ; Vaccination ; Vaccines, Inactivated ; immunology ; Viral Vaccines ; immunology