Radioactive (14)C-glucose(U) was given to Clonorchis sinensis in Tris buffer medium, in corder to trace the metabolic fate of the labelled carbon. The labelled carbon from glucose enters into intermediary metabolites and end products of anaeroblic glycolysis, Embden-Meyerhof pathway, and of aerobic Krebs cycle. These product were identified by one or two-dimensional paper chromatography in combination with autoradoigraphy. The labelled metabolites detected in this experiment corresponded to pyruvic acid, latic acid, malic acid, succinic acid and fumaric acid. Amino acids, such as alanine, aspartic acid, glutamic acid, valine, theronine, and serine, derived by the degradation of (14)C- glycose were also found. Labelled compounds behaving like alanine, aspartic acid and glutamic acid were observed in the chroma to gram of incubation medium. The preciptation which suggests a positive reaction for protein occured when absolute ethanol was added to the incubation medium.
parasitology-helminth-trematoda ; Clonorchis sinensis ; two-dimensional paper chromatography ; autoradiography ; metabolism ; glucose

No abstract available.
Kidney Transplantation*

INTRODUCTION: Bone mineral density (BMD) is an important index in diagnosis of osteoporosis and other metabolic bone diseases, prediction of fractures, and monitoring treatment. This study was to find a more feasible technique for prediction of osteoporotic fracture between dual energy X-ray absorptiometry (DXA) and quantitative computed tomography (QCT) and to reveal the actual change of bone strength when BMD was changed. METHODS: Ten of these 20 specimens were used as the demineralized group and the other 10 as the control. Each specimen was immersed in HCl solution at for a period of at least 10 minutes, up to 100 minutes, at an interval of 10 minutes for different levels of demineralization. BMD was measured using DXA and QCT. Uniaxial compression tests were conducted to measure biomechanical parameters. Pearson correlation analysis was used respectively between BMD and biomechanical parameters and between DXA and QCT. RESULTS: Elastic modulus (r=0.87) and yield stress (r=0.84) showed a statistically significant correlation with DXA BMD. Through correlation analysis with QCT BMD and elastic modulus, correlation coefficient showed hemi-vertebra (r=0.80) and trabecular (r=0.68). In yield stress, there was a statistically significant correlation in hemi-vertebra (r=0.87) and trabecular bone (r=0.84). CONCLUSION: DXA is a current standard technique not only for diagnosis of osteoporosis but also for prediction of fracture risk compared to QCT. Actual decrease of bone strength was much greater than that of BMD by both DXA and QCT.
Absorptiometry, Photon* ; Biomechanical Phenomena ; Bone Density* ; Bone Diseases, Metabolic ; Diagnosis ; Elastic Modulus ; Osteoporosis ; Osteoporotic Fractures

Collision tumors represent the coexistence of two adjacent but histologically distinct tumors in an organ. Collision tumors have been identified in various organs, but they are rare in liver. We present a rare case of a 57-year-old man, who was hospitalized for the removal of a liver mass (S5/6 segmentectomy), that was probably a hepatocellular carcinoma. The eventual pathology examination revealed a collision tumor composed of a hepatocellular carcinoma and an undifferentiated sarcoma. Because the tumor recurred 2 months after the operation, reoperation (right hemicolectomy and tumorectomy) was performed. In the 2nd month following the second operation we found multiple liver metastases, chest metastasis and abdominal cavity metastasis. To our knowledge this is the first case of a hepatic collision tumor that was composed of a hepatocellular carcinoma and an undifferentiated sarcoma. Herein, we report the case of a hepatic collision tumor and briefly review the literature.
Abdominal Cavity ; Carcinoma, Hepatocellular ; Humans ; Liver ; Middle Aged ; Neoplasm Metastasis ; Reoperation ; Sarcoma ; Thorax

No abstract available.
Child* ; Fistula* ; Humans

BACKGROUND: In 1951, Ardran reported that metastatic bone lesions could be detectable on plain radiography with 30% to 50% of decalcification. Authors performed experimental study for minimum level of decalcification to detect the osteolytic bone metastasis of long bone with recent technique of radiographs. METHODS: One pair of fibula and humerus from two cadavers was cut into specimen 1 inch in length. Distal half of specimen was dipped into hydrochloride (HCl) with 15 min interval. All 16 specimens were checked by film-type radiography (FR), computed radiography (CR), digital radiography (DR). To exclude inter-observer's variance, 3 radiologists evaluated images. Calcium amount before and after decalcification was measured and expressed in percentage of decalcification. RESULTS: Osteolytic changes were detectable with 11% to 16% of decalcification for fibula and 3% to 8% for humerus on plain radiography with FR, CR, and DR. CONCLUSIONS: Our study showed that minimum of 3% and maximum of 16% of decalcification is necessary when osteolytic metastatic bone lesions of long bone could be detected on plain radiography.
Cadaver ; Calcium ; Decalcification Technique ; Fibula ; Humerus ; Neoplasm Metastasis* ; Osteolysis ; Radiographic Image Enhancement ; Radiography*

PURPOSE: Of the various methods for evaluating erectile dysfunction, International Index of Erectile Function (IIEF) questionnaires and penile color Doppler ultrasonography are commonly performed. In this study, the total IIEF scores and erectile function domain scores of the IIEF were compared with the etiology diagnosed from the indices of penile color Doppler ultrasonography. MATERIALS AND METHODS: A total of 54 patients, with erectile dysfunction, who underwent penile color Doppler ultrasonography and IIEF, were retrospectively examined. Various blood flow parameters, including the peak systolic velocity, end diastolic velocity, mean flow rate and resistive index, were observed and recorded for about 30 minutes following an intracorporeal injection of alprostadil and self stimulation. According to the causes of the erectile dysfunction, as diagnosed with penile color Doppler ultrasonography, the total IIEF and erectile function domain scores of the IIEF were compared. RESULTS: There was no statistically significant difference in the total IIEF and IIEF scores of the erectile function domain between the causes of erectile dysfunction as diagnosed by the penile color Doppler ultrasonography. CONCLUSIONS: The IIEF scores did not statistically differentiate the specific etiologies of erectile dysfunction determined by penile color Doppler ultrasonography. Therefore, it is believed that the IIEF score alone cannot predict the etiologies of erectile dysfunction.
Alprostadil ; Erectile Dysfunction* ; Humans ; Male ; Surveys and Questionnaires ; Retrospective Studies ; Self Stimulation ; Ultrasonography, Doppler, Color

PURPOSE: Latissimus dorsi myocutaneous flap (LDMCF) is a commonly used technique for breast reconstruction following breast-conserving surgery. However, this technique has a high incidence of donor site seroma. The aim of this study is to evaluate the effect of donor-site quilting on seroma formation. METHODS: A retrospective review of 95 patients who underwent immediate breast reconstruction with LDMCF from May of 2006 through February of 2007 was performed. Patients were divided into Group A, in which only a closed suction drain was used, and Group B, in which quilting and a closed suction drain were used. The outcome measures were age, body mass index (BMI), mastectomy volume, duration of drain, total volume of postoperative seroma, length of hospital stay, and incidence of postoperative aspiration. RESULTS: In Group B, the total amount of seroma, duration of drain, and length of hospital stay were significantly reduced (P<0.05). However, the incidence of postoperative aspiration was not different between Group A and Group B (P=0.06). CONCLUSION: The quilting technique reduces the volume of postoperative seroma and may help prevent of seroma after LDMCF.
Body Mass Index ; Female ; Humans ; Incidence ; Length of Stay ; Mammaplasty ; Mastectomy ; Mastectomy, Segmental ; Outcome Assessment (Health Care) ; Retrospective Studies ; Seroma ; Suction ; Tissue Donors

PURPOSE: A lateral thoracodorsal fasciocutaneous flap (LTFF) is a local fasciocutaneous flap that has been used in breast reconstructions since the 1980s. Although the LTFF is a wellstudied reconstruction procedure after radical surgery in Western countries, there is no report in Korea. By introducing the LTFF procedure, we suggest an easy reconstruction technique that can be performed by the breast surgeon directly. METHODS: Patients with lateral breast cancer and redundant lateral thoracic region might be candidates for this procedure. The flap consists of the lateral and dorsal extensions of the inframammarian fold as well as an extended line from the anterior axillary line. A quadrantectomy is performed through a planned skin incision, and an axillary lymph node dissection can be performed simultaneously if the sentinel lymph node is positive. The skin and subcutaneous fat with the fascia of the serratus anterior and latissimus dorsi muscle should be dissected carefully. A wedge-shaped flap can be acquired successfully. The lateral breast defect is then reconstructed by a rotation of the flap. The axis of the flap is drawn following the inframammarian fold so that the final scar would be under the brassiere line. RESULTS: Nineteen patients were treated with the LTFF after breast conserving surgery. All tumors were located in lateral breast regions. Seroma occurred in three and partial fat necrosis and partial flap necrosis were observed in each one. The cosmetic result based on four-point scoring system of breast cosmesis showed excellent in seventeen and good in two. CONCLUSION: Despite its long scar line, with appropriate patient selection, a LTFF might be a useful method for breast reconstructions.
Axis, Cervical Vertebra ; Breast Neoplasms ; Breast* ; Cicatrix ; Fascia ; Fat Necrosis ; Female ; Humans ; Korea ; Lymph Node Excision ; Lymph Nodes ; Mammaplasty* ; Mastectomy, Segmental ; Necrosis ; Patient Selection ; Seroma ; Skin ; Subcutaneous Fat ; Superficial Back Muscles

Diffuse large B-cell lymphoma that arises from the gallbladder is extremely rare, and the associated studies are not well described in the literature. We report our experience that diffuse large B-cell lymphoma of the gallbladder was diagnosed by histological findings after laparoscopic cholecystectomy in a 75-year-old man. The patient was diagnosed with stage IV lymphoma, and chemotherapy was performed following surgery. The abdominal, chest, neck computed tomography (CT) and positron emission tomography (PET)-CT were performed after chemotherapy, and the results showed that there were no multiple lymphadenopathies. The patient was considered to have achieved complete remission. Diffuse large B-cell lymphoma of the gallbladder is extremely rare and never been diagnosis preoperatively. Pathological examination of the cholecystectomy specimen is important. This will be very helpful for identifying patients who need additional treatment.
Aged ; B-Lymphocytes ; Cholecystectomy ; Cholecystectomy, Laparoscopic ; Cholecystitis ; Diagnosis ; Drug Therapy ; Gallbladder ; Humans ; Lymphoma ; Lymphoma, B-Cell ; Neck ; Positron-Emission Tomography ; Thorax