No abstract available.
Crohn Disease*

It was well known that atrial myocytes systhesize atrial natriuretic peptide[ANP], and secrete it into the atrial lumen through the atrial endocardium. But the mechanism for regulation of ANP secretion has not been clearly elucidated, because there was little information of the atrial morphology concerning basal lamina. Basal lamina is surmised as one of barriers that control the movement of ANP, a large molecule. This study was attempted to elucidate the morphological characteristics of basal lamina and connective tissue fibers of atrial endocardial layer by scanning electron microscopy. Basal lamina was exposed by removal of the overlying endothelium. This was achieved by using OsO4 maceration, immersion in aqueous boric acid or EDTA treatment. After removal of the endothelial cell, the specimens were exposed to ultrasonic vibration in case of need. The external surface of basal lamina showed a fairly smooth appearance on the whole, although a few irregular folds are often encountered. Fenestrations, 0.1-1 micrometer in diameter, were randomly observed on the basal lamina, and they were circular to oval in shape. Margin of fenestrations was somewhat distinct and some was divided into two parts by linear structures. The structural differences of fenestrations between right and left atria were not found. The fibroreticular lamina under the basal lamina was revealed by removal of the endothelial cells and their basal lamina. This layer was consisted of interwoven fine fibers. These fine fibers were repeatedly divided and fused, forming reticular network. Some fine fibers connected with basal lamina. Some connective tissue fibers below fibroreticular layer were collected into thick bundles running parallel to myocytes. Above results may serve as a basis for the physiological and morphological studies of atrium.
Animals ; Atrial Natriuretic Factor ; Basement Membrane* ; Connective Tissue ; Edetic Acid ; Endocardium* ; Endothelial Cells ; Endothelium ; Immersion ; Microscopy, Electron, Scanning ; Muscle Cells ; Rats* ; Running ; Ultrasonics ; Vibration

No abstract available.
Pyelonephritis, Xanthogranulomatous* ; Wilms Tumor*

The purpose of this paper is to investigate the extent of destruction Blood-ocularbarrier between colored rabbits and white rabbits after photocoagulation, as preliminary experiment for the using colored and white rabbits. A total of 10 rabbits (5 white rabbits, 5 colored rabbits) were used. Photocoagulation in the right eye of 5 white rabbits and 5 colored rabbits were performed. The left eye of all the rabbits was control eye and the right eye was laser treated eye. After 3 days, a dose of 25mg/kg of 10% fluorescein sodium was injected in the ear vein of rabbit intravenously. We sampled aquous humor in anterior chamber and vitreous, and checked fluorescein concentration in anterior chamber and vitreous using HPLC (High Performance Liquid Chromatography). The results obtained were as follows; In experimental animals, 1. There is no significance between colored rabbits and white rabbits in fluorescein concentration of vitreous and aquous humor in control group. 2. The concentration of the fluorescein in the anterior chamber and vitreous of the right photocoagulated eye was higher than control eye, but there is no significance between colored rabbits and white rabbits. 3. there is no significance between colored rabbits and white rabbits in extent of destruction of Blood-ocular-barrier after Laser photocoagulation.
Animals ; Anterior Chamber ; Chromatography, High Pressure Liquid ; Ear ; Fluorescein ; Light Coagulation ; Rabbits* ; Veins

We present here, a case of pemphigus erythematosus developed in a 31-year-old woman, who had been suffering from repeated episode, of remission and exacerbation with several erythematous, and somewhat oozing patche accompanying a mild pruritus or her face, upper part of the back and anterior chest. In serological studies, she had antinuclear antiboc@lies at. a titer of 1:160. Histopathological finding showed subcorneal bullae. Immunofluorescent study revealed IgG and C, deposition in the intercellular space of the epidermis and along the de rmoepidermal junction.
Adult ; Epidermis ; Extracellular Space ; Female ; Fluorescent Antibody Technique ; Humans ; Immunoglobulin G ; Pemphigus* ; Pruritus ; Thorax

Pemphigua foliaceus is a rare, relatively mild and chronic variety of pemphigus which is characterized by superficial flaccid bullae which show acantholytic changes in the upper part of the epidermis histopathologically. We report herein five cases of pemphigus foliaceus. The patients had erythematous and crusted patches chiefly on face, chest and back areas, except for case 2 who had flaccid bullae on the upper extrimities. The diagnosis of all these patients were confirmed by immunofluorescence studies. Among these five patients, one was recognized as a rifampicin induced pemphigus foliaceus, and the other one revealed concurrent lesions of psoriasis.
Diagnosis ; Epidermis ; Fluorescent Antibody Technique ; Humans ; Pemphigus* ; Psoriasis ; Rifampin ; Thorax

No abstract available.
Kidney Transplantation*

No abstract available.
Phonation* ; Tooth*

The primary purpose of the study is to investigate the destruction and recovery of the blood retinal barrier after photocoagulation and cryotherapy in the rabbits. The 110 pigmented rabbits were used in this experiment. After photocagulation and cryotherapy we injected intravenousely a dose of 25 mg/kg of fluorescein sodium, and then sampled blood and vitreous and measured the concentration of fluorescein sodium by means of High Performance Liquid Chromatography. The calculated penetration ratio which indicates the destruction of the blood retinal barrier is obtained by dividing fluorescein sodium concentration of vitreous by integral of fluorescein sodium concentration of the plasma from 3 min to 60 min. Subsequently, fundus photography and enucleation for flat preparation were performed in each experimental rabbit. The fluorescein concentration of vitreous of the normal rabbit is 3.60 +/- 4.75 X 10(-9)gm/ml and its penetration ratio is 0.20 +/- 0.23 X 10(-6) min. After measuring the correlation between the frequency of photocoagulation and penetration ratio and between the frequency of cryotherapy and penetration ratio, we found out that the correlation coefficient was 0.885 and 0.909 respectively. And this experiment showed that penetration ratio was higher in cryotherapy group than in photocoagulation group. In addition, we divided these experimental animals into 4 groups, mild and severe cryotherapy groups and lighter and heaver photocoagulation groups, and assessed the penetration ratio of these four groups at 1,3,7,14,28, and 42 days after treatment. As a result, penetration ratio was highest at 3 days after treatment and was almost back to normal by 42 days in all experimental groups except in severe cryotherapy group. Compared with photocagulation groups, cryotherapy groups showed more extensive destruction and delayed recovery of the blood retinal barrier. In fundus photography, in the photocoagulation groups white patch was developed after treatment, at 7 days white patches disappeared and were replaced by pigmented and scarred tissue; by 42 days the margin of these lesions was indistinct and the lesions were changed into relatively small scarred patches. On the other hand, in the cryotherapy groups the thick round white patch wasdeveloped after treament, at 7 days large round white patches disappeared and were replaced by pigmented and scarred tissue; by 42 days the lesions were replaced by large scarred patches with pigmentation and depigmentation. In flat preparation, in the photocoagulation groups central necrotic zone and intermediate zone with hyperpigmentation and peripheral zone with hypopigmentation was presented at 1 day. In the cryotherapy groups the diminished density, in the center and white ring at the margin was revealed at 1 day. From 7 days in photocogulation groups and from 14 days in the cryotherapy groups, retinal pigment epithelium began to show proliferation and by 42 days, retinal pigment epithelial layer of both groups except severe cryotherapy group was replaced by relatively normal retinal pigment epithelium.
Animals ; Blood-Retinal Barrier* ; Chromatography, Liquid ; Cicatrix ; Cryotherapy* ; Fluorescein ; Hand ; Hyperpigmentation ; Hypopigmentation ; Light Coagulation* ; Photography ; Pigmentation ; Plasma ; Rabbits* ; Retinal Pigment Epithelium ; Retinaldehyde

Respiratory distress syndrome (RDS) of preterm infants remains a significant cause of morbidity and mortality despite improvements in neonatal intensive care and artificial ventilatory techniques. After identification of the deficiency of pulmonary surfactant is major pathophysiologic basis in RDS, artificial surfactant replacement therapy in RDS was first successfully tested by Fujiwara and co-workers in 1980. therefore, exogenous surfactant replacement produced exellent results in improved clinical and repiratory status during the acute period and decreased incidence of late complications and mortality. According to comparison of administration timing between early (within 6 hours after birth) and late (after 6 hours)group, early replacement therapy is more effective in improving of clinical course and prognosis. Because of that, early, just after birth, recognition and detection of RDS is also important procedure. There are many investigations and methods for the detection of RDS in prenatal or postnatal period. Among then, stable microbubble rating (SMR) test was a simple method and SMR test has a higher diagnostic accuracy. To determine the relation of the SMR and purified natural surfactant (PNS) concentration in vitro, the author conducted each 5 times test of SMR method according to 5 groups of PNS concentration by using modified Pattle's method. The results were as follows: 1) The mean and standard deviation of SMR according to 5 groups of PNS concentration were 119.4 (15.0in 20mug PL (phospholipid)/ml, 452.2 (160.2 in 40mug PL/ml, 879.0 (93.4 in 60mug PL/ml, 1311.8 (274.8in80mug PL/ml, 1710.6(272.3 in 100mug PL/ml. 2) The regression curve of SMR and PNS concentration showed statistically significant relation(p<0.005). In conclusion, the SMR test was a good method in estimation of surfactant concentration in vitro and also in diagnosis of RDS recognized as a surfactant deficiency. In the future, we expected that prophylactic surfactant replacement therapy. immediate after birth, will be more popular in the field of neonatal care of RDS. So, we recommended the use of this method for early detection and serving optimal care of RDS.
Diagnosis ; Humans ; Incidence ; Infant, Newborn ; Infant, Premature ; Intensive Care, Neonatal ; Microbubbles* ; Mortality ; Parturition ; Prognosis ; Pulmonary Surfactants*