The extracellular cytolysin produced by V. ulnificus has gained great attention as a causative factor for the pathogenesis of V. vulnificus-infected disease. In this study, an attempt was made to elucidated the mechanism for the toxity of cytolysin. 1. Cytolysin given to mouse showed lethal activity with LD of 3.7 HU and produced hemoconcentration, suggesting that its lethal activitis attributable to the increased vascular permeability. 2. The lethal activity of cytolysin for mouse was prevented by intraperitoneal administration of clemastin or verapamil one hour before intravenous injection of cytolysin. 3. Treatment of peritoneal mast cells from rats with cytolysin stimulated histamine relesse irrespective of the prescence of extracellular Ca and the stimulatory effect of cytolysin was not affected by the pretreatment of mast cells with per tussis toxin. 4. The hemolytic activity and stimulatory effect of cytolysin on histamine release from mast cell were inhibited by cholesterol. The results suggest that the lethal activity of cytolysin is attributable to the increased vascular permeability which is resulted from the stimulation of histsmine release from mast cells and/or basophils by cytolysin.
Animals ; Basophils ; Capillary Permeability ; Cholesterol ; Histamine ; Histamine Release ; Injections, Intravenous ; Mast Cells ; Mice ; Perforin* ; Rats ; Verapamil ; Vibrio vulnificus* ; Vibrio*

Takayasu's arteritis is briefly as an inflammatory process of unknown etiology occuring dominantly in young female most commonly involving the great vessels arising from the aortic arch. The disease process results in stenosis and obliteration of involved vessels. Syncope in Takayasu's arteritis is known to result from decreased blood flow to brain because of stenosing blood vessels. We herein describe a case of paroxysmal atrioventricular block in a 40-year old man with Takayasu's arteritis. The patient presented with spontaneous recurrent syncope associated with paroxysmal AV block. Carotid sinus pressure repeatedly induced paroxysmal AV block and syncope. Carotid sinus pressure after pretreatment of atropine failed to induce AV block or syncope. Coronary angiogram was normal. We concluded that syncope in this patients was attributed to carotid hypersensitivity rather than decreased blood flow to the brain due to vessel stenosis.
Adult ; Aorta, Thoracic ; Atrioventricular Block* ; Atropine ; Blood Vessels ; Brain ; Carotid Sinus ; Constriction, Pathologic ; Female ; Humans ; Hypersensitivity ; Syncope ; Takayasu Arteritis*

In this paper, course and complications of the 200 cases of cataract extractions and postoperative period as well as the visual acuity a t conclusion of period of observation are presented. The observation are made comparing with the types of corneo-scleral suture. The vision of preoperatively complicated cases are not so improved after successful cataract extraction. Vitreous loss during the operation and, vitreous protrusion into anterior chamber do not interfere thc post-operative vision. Massive and longstanding hyphema used to interfere the final vision. The average visual acuity after the operation revealed 0.34. All the above indicated observation are performed on the cases of average age 56.1 years old.
Anterior Chamber ; Cataract Extraction* ; Cataract* ; Dronabinol ; Hyphema ; Postoperative Period ; Sutures ; Visual Acuity

Retroperitoneal fibrosis sometimes causes urological problem involving the ureter, but the mechanism is uncertain. An aortic aneurysm, including an iliac artery aneurysm, is thought to be one of the mechanisms of retroperitoneal fibrosis. However, cases caused by an isolated iliac artery aneurysm are very rare, and symptoms tend to be non-specific; therefore, no definitive treatment has been established. Herein, we report our recent experience of a patient with a left common iliac artery aneurysm involving the ureter, who underwent successful surgical therapy.
Aneurysm* ; Aortic Aneurysm ; Constriction, Pathologic* ; Humans ; Iliac Artery* ; Retroperitoneal Fibrosis* ; Ureter*

No abstract available.
Humans

The ornithine aminotransferase (OAT) activity of mouse was found to be highest in the small intestine. The mitochondrial OAT from mouse small intestine was purified to homogeneity by the procedures including heart treatment, ammonium sulfate fractionation, octyl-Sepharose chromatography, and Sephadex G-150 gel filtration. Comparing to the amino acid sequence of mouse hepatic OAT, six N-terminal amino acid residues have been deleted in intestinal OAT. However, the subsequent sequence was identical with that of hepatic OAT. The molecular weights of both intestinal and hepatic OAT were estimated as 46 kDa by SDS-gel electrophoresis and as 92 kDa by gel filtration, indicating that both native OATs are dimeric. Biochemical properties of intestinal OAT, such as molecular weight, pH optimum and K(m) values for L-ornithine and alpha-ketoglutarate, were similar to those of hepatic OAT. However, intestinal OAT was more labile than hepatic OAT to tryptic digestion.
Amino Acid Sequence ; Animal ; Intestine, Small/enzymology* ; Liver/enzymology ; Male ; Mice ; Mice, Inbred ICR ; Molecular Sequence Data ; Molecular Weight ; Ornithine-Oxo-Acid Transaminase/metabolism* ; Ornithine-Oxo-Acid Transaminase/isolation & purification ; Ornithine-Oxo-Acid Transaminase/genetics* ; Tissue Distribution ; Trypsi

The systolic time intervals were measured in 25 normal controls and 23 patients with dilated cardiomyopathy by simultaneous recording of the aortic valve echocardiogram and ECG. These values were compared to corresponding ones obtained from the method using simultaneously recorded phonocardiogram, ECG, and indirect carotid pulse tracings. And we assessed left ventricular function by systolic time intervals in dilated cardiomyopathy. The results were as followings. 1) High degree of correlation(r> or =0.94) was found between the two methods for each intervals, EMS, LVET, PEP, PEP/LVET. 2) In normal controls, PEP/LVET obtained from echocardiographic measurement was 0.31+/-0.02. 3) In the patients with dilated cardiomyopathy, PEP/LVET(0.59+/-0.13) was significantly higher(p<0.001), PEP index was longer(p<0.05), LVET index was shorter(p<0.05) than in normal controls.
Adult* ; Aortic Valve ; Cardiomyopathy, Dilated* ; Echocardiography* ; Electrocardiography ; Humans ; Systole* ; Ventricular Function, Left

Glucose prevents the development of diabetes induced by alloxan. In the present study, the protective mechanism of glucose against alloxan-induced beta-cell damage was investigated using HIT-T 15 cell, a Syrian hamster transformed beta-cell line. Alloxan caused beta-cell damages with DNA fragmentation, inhibition of glucose-stimulated insulin release, and decrease of cellular ATP level, but all of these beta-cell damages by alloxan were prevented by the presence of 20 mM glucose. Oligomycin, a specific inhibitor of ATP synthase, completely abolished the protective effects of glucose against alloxan-induced cell damage. Furthermore, treatment of nuclei isolated from HIT-T15 cells with ATP significantly prevented the DNA fragmentation induced by Ca2+. The results indicate that ATP produced during glucose metabolism plays a pivotal role in the protection of glucose against alloxan-induced beta-cell damage.
Adenosine Triphosphate/pharmacology ; Adenosine Triphosphate/metabolism ; Alloxan/pharmacology* ; Animal ; B-Lymphocytes/metabolism ; B-Lymphocytes/drug effects* ; B-Lymphocytes/cytology ; Calcium/pharmacology ; Cell Line ; Cell Nucleus/genetics ; Cell Nucleus/drug effects ; Cell Survival ; DNA/metabolism ; DNA/genetics ; DNA/drug effects ; DNA Fragmentation ; Dose-Response Relationship, Drug ; Egtazic Acid/pharmacology ; Glucose/pharmacology* ; Insulin/secretion ; Oligomycins/pharmacology

No abstract available.
Neck*

No abstract available.
Nasal Surgical Procedures*