BACKGROUND: The proliferation of vascular smooth muscle cells(VSMCs) is an important process in the pathogenesis of atherosclerosis. VSMCs proliferation is closedly related to the cytosolic calcium flux via L-type voltage dependent calcium channel. OBJECTIVES: To investigate the role of cytosolic Ca2+ in the proliferation of VSMCs. METHODS: The proliferation of aortic vascular smooth muscle cells of streptozotocin induced diabetic rats, acquired by enzymatic method. Cell counting, and calcium agonists(Bay K 8644 10(-6)M) or calcium antagonists(verapamil 10(-6)M). Cytosolic calcium ion was measured with Fura 2 method. Calcium channel gene expression was detected with RT-PCR method. RESULTS: VSMCs of diabetic rats were more proliferated than those of nondiabetic ones. Bay K 8644 enhanced VSMCs proliferation of both groups. Verapamil blocked the incremental effects induced by Bay K 8644. Expression of calcium channel gene was enhanced by Bay K 8644 and was reversed by vera- pamil. CONCLUSION: The enhanced proliferation of VSMCs is associated with the increment in cytosolic calcium, which is accompli shed through the expression of L-type voltage dependent calcium channel.
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester ; Animals ; Aorta* ; Atherosclerosis ; Calcium ; Calcium Channels ; Cell Count ; Cytosol ; Fura-2 ; Gene Expression ; Muscle, Smooth, Vascular* ; Rats* ; Streptozocin ; Verapamil

No abstract available.
Progeria*

No abstract available.
Aging*

No abstract available.
Diabetes Mellitus* ; Drug Therapy*

No abstract available.

No abstract available.
Aging*

No Abstract Available.
Endocrinology*

BACKGROUND: Vascular smooth muscle cells (VSMCs) proliferation is an important process in the pathogenesis of atherosclerosis. VSMCs proliferation is closedly related to the cytosolic calcium flux via L-type voltage dependent calcium channel. OBJECTIVES: To investigate the role of cytosolic Ca(2+) in the proliferation of VSMCs. METHODS: The proliferation of aortic vascular smooth muscle cells of rats(1% cholesterol diet fed rats and general diet fed ones), acquired by enzymatic method. Cell counting, and calcium agonists(Bay K 8644 10(-6)M) or calcium antagonists(verapamil 10(-6)M). Cytosolic calcium ion was measured with Fura 2 method. Calcium channel gene expresssion was detected with RT-PCR method. RESULTS: Masson Trichrome staining shows more disturbed cell lining, more VSMCs, more degenerated media, more collagen laydown, and more adventitial fat in the aorta of cholesterol-fed rats than in that of general diet-fed ones. VSMCs of cholesterol fed-rats were moreproliferated than those of general diet fed-ones. Bay K 8644 enhanced VSMCs proliferation of both groups. Verapamil blocked the incremental effects induced by Bay K 8644. Expression of calcium channel gene was enhanced by Bay K 8644 and was reversed by verapamil. CONCLUSION: The enhanced proliferation of atherosclerotic VSMCs is associated with the increment in cytosolic calcium, which is accomplshed through the expression of L-type voltage dependent calcium channel.
3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester ; Animals ; Aorta* ; Atherosclerosis ; Calcium Channels ; Calcium* ; Cell Count ; Cholesterol* ; Collagen ; Cytosol* ; Diet ; Fura-2 ; Muscle, Smooth* ; Muscle, Smooth, Vascular ; Myocytes, Smooth Muscle* ; Rats* ; Verapamil

No abstract available.
Aged* ; Diabetes Mellitus* ; Humans

No abstract available.
Constipation*