No abstract available.
Animals ; Isoproterenol* ; Nitric Oxide* ; Rats* ; Urinary Bladder*

PURPOSE: We investigated the possible inhibitory effect of aspirin during the initiation and post initiating stages on N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) induced bladder carcinogenesis in female rats. MATERIALS AND METHODS: Forty-five female Sprague-Dawley rats were divided into three groups. Group A received 0.05% BBN in drinking water for 12 weeks. Group B re ceived 0.05% BBN in drinking water with 0.5% aspirin in the diet for 12 weeks. Group C received control diet without added chemicals. The rats were sacrificed after 12 weeks. For identification of chemopreventive effect of aspirin, apoptosis was detected by in situ cell death detection method. The apoptotic index (AI) was calculated from the ratio of typical apoptotic cells relative to the total cells from observation of at least 1000 cells in each preneoplastic lesion among the groups. RESULTS: All cases of group A showed multiple lesions of simple and focal hyperplasia. The incidence of papilloma was 80% in group A. All cases of group B also showed multiple lesions of simple and focal hyperplasia but the incidence of papilloma was decreased to 20%. The total numbers counted of focal hyperplasia and papilloma lesions were significantly reduced in group B than in group A (p<0.001 and p<0.001, respectively). The mean AI in group A and B sequentially increased in preneoplastic lesions, as compared to that in the normal epithelium of the rat bladder. Significant differences in AI in the lesions of simple and focal hyperplasia between group A and B were noted (p<0.001 and p<0.001, respectively). CONCLUSIONS: The result suggests that aspirin significantly decreases the incidence of precancerous lesions and it can act as a chemopreventive agent for precancerous lesions of urinary bladder.
Animals ; Apoptosis ; Aspirin* ; Carcinogenesis ; Cell Death ; Diet ; Drinking Water ; Epithelium ; Female ; Humans ; Hyperplasia ; Incidence ; Papilloma ; Rats* ; Rats, Sprague-Dawley ; Urinary Bladder Neoplasms ; Urinary Bladder*

The cause of radiolucent filling defects in the upper urinary tract are malignant tumor, radiolucent stones, blood clots, air bubbles, congenital deformities of renal parenchyme, and various specific and non-specific infection and their sequence. So the differential diagnosis between malignancy and radiolucent stones is very important, and the exact and fast diagnosis of radiolucent stones is useful in excluding the possibility of malignancy. 27 cases with radiolucent stones were evaluated retrospectively for exact diagnosis and appropriate treatment method. Intravenous urography was done in all cases, and retrograde pyelography, ultrasonography, computed tomography and/or ureterorenoscopy were performed for diagnosis, if needed. Size of stones were measured below 10mm by transverse length in 19 cases (70.4%) with a range of 3 to30mm. The locations of stones were pelvocalyceal system in 8 cases (29.6%) and ureter in 19 cases (70.4%). Among the diagnostic methods, computed tomography was used most frequently. ESWL with retrograde pyelography was most frequent used method of treatment, also simple hydration to small stone was effective.
Congenital Abnormalities ; Diagnosis ; Diagnosis, Differential ; Retrospective Studies ; Ultrasonography ; Ureter ; Urinary Tract ; Urography

No abstract available.
Animals ; Rats* ; Superoxides*

PUROPOSE: Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is a common disorder to men and the syndrome is diagnosed only on the basis of symptoms, principally pain or discomfort in the pelvic region. However many patients have a urinary symptoms, especially storage symptoms such as frequency and urgency. The aim of this study was to evaluate the efficacy of anticholinergics in a prospective randomized, single-blind trial to young and middle aged patients to exclude the effect of benign prostatic hyperplasia as possible. MATERIAL AND METHODS: Forty six men with CP/CPPS (age of third to fifth decades) were randomized in a single-blind fashion to receive either group 1; gatifloxacin (200mg, bid) of 15 patients, or group 2; gatifloxacin (200mg, bid) and propiverine (20mg, once daily) of 31 patients for 2 months. The NIH chronic prostatitis symptom index (NIH-CPSI) and International Prostate Symptom Score (IPSS) were used to grade symptoms and the quality of life (QoL) impact at the start, 1 month and 2 months of the study. RESULTS: There was no significant difference between group 1 and group 2 in about age and duration of the disease. In addition, no significant difference was found between group 1 and 2 in the scores of sub-factors of IPSS and NIH-CPSI at the time of baseline. No statistically significant difference in the NIH-CPSI total score and each domain was seen after treatment. Statistically significant difference in the storage symptom of IPSS was seen after treatment. CONCLUSIONS: The efficacy of propiverine (combined to antibiotics) for CP/CPPS were improvements of the storage symptom of IPSS significantly.
Cholinergic Antagonists ; Humans ; Male ; Middle Aged ; Pelvic Pain* ; Pelvis ; Prospective Studies ; Prostate ; Prostatic Hyperplasia ; Prostatitis* ; Quality of Life

PURPOSE: The aim of this study was to investigate the effects of testosterone deprivation on urinary bladder in male rabbits by proteomic analysis. MATERIALS AND METHODS: New Zealand white male rabbits (2.5-3 kg) were divided into 2 group; control group with 5 rabbits and bilateral orchiectomized group, bilateral orchiectomized group was divided into post-operative 4 weeks group (group 1), and 8 weeks group (group 2) with 5 rabbits respectively. Bladder wall was excised partly at 4 or 8 weeks from the beginning of the experiment. Conventional proteomics was performed with high resolution 2-D gel electrophoresis followed by computational image analysis and protein identification using mass spectrometry. We decided to consider 'significant' if protein had 50% decreasing or 200% increasing expression rate. RESULTS: Six proteins were significantly changed in orchiectomy group, compared to control group; serum albumin precursor, GABA transaminase, dimethylarginine dimethylaminohydrolase 2, serum/glucocorticoid regulated kinase and LOC304923 protein were over-expressed in both group 1 and group 2. Annexin A1 was significantly over-expressed in the group 2 only. CONCLUSION: An overexpression of Annexin A1 and GABA transaminase mean a processing of neuronal reactions to injury in orchiectomized rabbit bladder. Also an overexpression of dimethylarginine dimethylaminohydrolase 2 would increase NO synthesis and thereby promote re-endothelialization in the rabbit bladder after orchiectomy. Serum/glucocorticoid regulated kinase would relate to Na+ transport. Albumin precursor and LOC304923 protein are remained to further research. These data suggested that bilateral orchiectomy would make the urinary bladder unstable. However more information is needed in human bladder tissue.
4-Aminobutyrate Transaminase ; Annexin A1 ; Electrophoresis, Gel, Two-Dimensional ; Humans ; Male ; Mass Spectrometry ; Neurons ; New Zealand ; Orchiectomy* ; Phosphotransferases ; Proteomics ; Rabbits ; Serum Albumin ; Testosterone ; Urinary Bladder*

PURPOSE: To explore the effect of bilateral oophorectomy on the several proteins of female rat bladder via a proteomic approach. MATERIALS AND METHODS: A total of 20 female Sprague-Dawley rats were obtained at 8 weeks of age and were divided randomly into 3 groups: a control groups underwent sham operation and bladders were excised 4 weeks and 8 weeks after operation each. Other two groups underwent bilateral oophorectomy and bladders were excised 4 weeks (group 1) and 8 weeks (group 2) after operation each, too. Each group has 5 rats. Conventional proteomics was conducted via high resolution 2-D gel electrophoresis, followed by image analysis and protein identification through mass spectrometry. RESULTS: Bladder weights were reduced significantly in group 2 as compared with the 8-week control group. A comparison of the bladders of the oophorectomy group subjects with those of the control group subjects demonstrated that the expressions of 11 proteins were altered Eukaryotic translation initiation factor 5A (elF-5A), chaperone grp 75 precursor, actin-depolymerizing factor, contrapsin-like inhibitor 1 precursor, guanine deaminase, actin, peroxiredoxin 2, phosphatidylethanolamine, putative protein kinase, Keratin complex 2 and Enol protein. CONCLUSION: The roles of 11 proteins are variable. A part of these proteins have a role of cellular apoptosis, acute inflammation, and muscle contraction. It seems that these changes of proteins have an influence on bladder functions and LUTS.
Actins ; Animals ; Apoptosis ; Electrophoresis, Gel, Two-Dimensional ; Female ; Guanine Deaminase ; Humans ; Inflammation ; Mass Spectrometry ; Muscle Contraction ; Ovariectomy* ; Peptide Initiation Factors ; Peroxiredoxins ; Protein Kinases ; Proteomics ; Rats* ; Rats, Sprague-Dawley ; Urinary Bladder* ; Weights and Measures

PURPOSE: Nerve root compression from spinal stenosis or herniated intervertebral disc(HIVD) may affect bladder function by the disease itself, operation or inappropriate general postoperative care. Preoperative urodynamic study seems to be needed for differential diagnosis of postoperative bladder dysfunction. MATERIALS AND METHODS: Thirty three patients diagnosed as nerve root compression were analysed for urodynamic features prospectively and classified into type I(hyporeflexic, hyposensitive, normal sphincter and hypocontractile, high compliance), type II(hyperreflexic, hypersensitive, normal sphincter and hypocontractile, low compliance), type III(hyperreflexic, hypersensitive, normal sphincter and hypercontractile, low compliance) and normal. Correlation with voiding symptoms, duration of the disease and urodynamic findings was evaluated. RESULTS: The mean age of patients was 56.2 years(29-80 years old), including 21 men and 12 women. Among thirty three patients, 21 had spinal stenosis and twelve patients were diagnosed as HIVD. Urodynamically, there were 16 cases(48.5%) showing type II, 6 cases(18.2%) for type I and 2 cases(6%) for type III. Normal finding was shown in 9 cases(27.3%). No significant difference was shown in mean duration of disease and in urodynamic findings. Patients with voiding symptoms or abnormal urodynamic finding were 20 cases(60.6%) and 24 cases(72.7%), respectively. Among thirteen patients without voiding symptoms, eight patients(61.5%) had abnormal urodynamic findings. CONCLUSIONS: Voiding symptoms and urodynamic findings in patients with nerve root compression were various. Because there is no consistent pattern of dysfunction with any type of nerve root compression disease, preoperative urodynamic study seems to the cornerstone for rational therapy and gives the exact information of bladder function to orthopedic operator.
Diagnosis, Differential ; Female ; Humans ; Male ; Orthopedics ; Postoperative Care ; Prospective Studies ; Radiculopathy* ; Spinal Stenosis ; Urinary Bladder ; Urodynamics*

Lysis of epidural adhesion has been done in patients with refractory lumbar radiculopathies. Cauda equina syndrome is a rare complication of epidural block. We report a case of cauda equina syndrome following epidural adhesiolysis in a patient with spinal stenosis. She complained of numbness in perineum, weakness of left leg, voiding difficulty, and fecal incontinence. She was finally recovered 1 month after the procedure without any sequelae following treatment with medication, bladder training and physical therapy. We suggest the causative factors are osmotic damage produced by subarachnoid injection of large doses of hypertonic saline and the temporary neural compression due to spinal stenosis.
Fecal Incontinence ; Humans ; Hypesthesia ; Leg ; Perineum ; Polyradiculopathy* ; Radiculopathy ; Spinal Stenosis* ; Urinary Bladder

No abstract available.
Hamartoma*