The present study was undertaken to evaluate and compare the in vitro leucoagglutination titers and in vivo lymphopenic eftect of three different antilymphocytic sera. Rabbits were immunized against adult dog and pup lymphoid tissue with multiple intramuscular injections of pooled spleen, mesenteric lymphnode and thymus cells. The number of lymphoid cells per each immunization was 1.8 * 107 of spleen cells, 1.68*108 of mesenteric lymphnode cells and 4.4*107 of thymus cells and immunization was done once per 10 days for 2 months. After completion of immunization and one booster injection, the rabbits were bled and total of 11 antidog spleen sera(RADSS), 4 antidog lymphnode sera(RADLS)and 3 antidog thymus sera (RADTS) were prepared and stored at-20C until used. With in vitro agglutination technique, each serum was serially diluted with buffered saline and tested with freshly prepared dog spleen, lymphnode,thymus cells and peripheral blood buffy coat leucocytes. With in vivo test, the antilymphocytic sera were intramuscularly injected in a dose of 1 ml/kg into adult dogs and after 12 hours peripheral blood pictures were evaluated and compared with those before antisera administration. The results were as follows. 1. The range of in vitro leucoagglutination titers of RADSS for spleen cells, tymphnode cells, thymus cells and peripheral leucocytes was 1: 4 to 1: 128, 1: 4 to 1: 128, 1: 4 to 1: 64 and 1: 4 to 1: 128, respectively. 2. The range of in vitro leucoagglutination titers of RADLS for sp]een cells, lymphnode cells, thymus cells and peripheral leucocytes was 1: 32 to 1: 64. 1: 32 to 1: 256, 1: 32 to 1: 128 and 1: 16 to 1: 256, respectively. 3. The range of in vitro leucoagglutination titers of RADTS for spleen cells, lymphnode cells, thymus cells and peripheral leucocytes was 1: 16 to l: 128, 1: 16 to 1: 64, 1: 8 to 1: 64 and 1: 8 to 1: 64, respectively. 4. After a singIe intramuscular injection of the antisera, the total R.B,C. counts were not significantly changed in RADSS, RADLS and RADTS. However, the percentage of neutrophils increased bv a mean value of 35.3% 34.8% and 21.7%p in RADSS,RADI.S and RADTS, respectively and the percentage of lymphocytes decreased by a mean value of 47.8%, 51.8% and 30.6% in RADSS, RADLS and RADTS, respectiveIy. The eosinophils showed a tendency of decrease in RADSS, RADLS and RADTS, respectively. The monocyes and basophis were not significantly changed after injection of the antisera. The author found that there was no significant difference between the in vitro leucoagglutination titers of three kind of antisera and that with in vitro leucoagglutination test, the peripheral blood leucocytes may serve as an useful antigen because of simp]icity of preparation. Also, the author found that there was no direct correlation between the in vitro leucoagglutination titers and in vivo Iymphopenic effect, but generally the antisera with strong lymphopenic effect had higher in vitro titers than those with weak lymphopenic effcct, In this experiment, the lymphopenic effect of RADTS was slightly lawer than those ot RADSS and RADLS and this difference may be due to smaller number of the cells used in immunization for the preparation of the antisera. The author concluded that spleen was as useful as lymphnode or thymus in its antigenicity and in producing effective antilymphocytic serum.
Adult ; Agglutination ; Animals ; Blood Buffy Coat ; Dogs ; Eosinophils ; Humans ; Immune Sera ; Immunization ; Injections, Intramuscular ; Lymphocytes ; Lymphoid Tissue ; Neutrophils ; Rabbits ; Spleen* ; Thymus Gland*

1) A case of Behcets syndrome in 45th years old house-wife was presented. 2) The patient well responsed to the administration of Antibiotics or Antihistaminics. 3) Author reviewed of the related literature.
Anti-Bacterial Agents ; Behcet Syndrome* ; Humans

No abstract available.
Incontinentia Pigmenti*

BACKGROUND: Hydrofluoric acid(HF) is one of the strong stirritating, corrosive and poisonous inorganic acid, The HF burns are occuring with ever-increa firifrequency due to the wide use of this acid in industrial fields, such as electronics and semicor tor industries. OBJECTIVE: The purpie of this study is to quantiate theound depths produced by varying concentrations of HF aiud to suggest the treatment guideline. METHODS: Uarious cinicentrations of HF(5%, 25%, 50%),were applied to two pairs of test sites on a guinea pig's back in a uniform fashion with cotton-tip applicators and then the histopathologic changes of guinea pig skin were observed. RESULTS: 1, In the 5% HF applied group, intracellular edema vaobserved in the epidermis after 12 hours. After 3days, epidermal cell necrosis was observed. 2. In the 25% HF applied group, intraepidermal vesice was observed after 6hours. After 12hours, inflammatory cell infiltrations were observed in the apper dermis and then after 3days, dermal necrosis was oberved. 3. In the 50% HF applied group, epidermal cell necros After 6hours, liquefaction degeneration in the epidermis an was observed focally after 3hours. iflhmmatory cell infiltrations in the upper dermis were observed. After 2days, dermalar.ecrosis was observed and then after days, the reaction was at its peak and subcutaneous tissuc. necrosis was observed. CONCLUSION: This study showed that a higher concentrator HF exposure enhances The penetration of HF. For treatmi not alcohloic solution of a quarternary ammonium for avintralesional injection of calcium gluconate within 24 hours of HF and a longer period after we suggest wet dressing with an concentration of HF burn and high concentration of HF burn.
Ammonium Compounds ; Animals ; Bandages ; Burns* ; Calcium Gluconate ; Dermis ; Edema ; Epidermis ; Guinea Pigs* ; Guinea* ; Hydrofluoric Acid* ; Necrosis ; Skin

BACKGROUND: Althogh drug euption refers to a common dermatological condition, there are numerous holdbacks on the diagnosis. OBJECTIVE: This study atternpts to define the clinical features and causavite drug of the patients with drug eruptions, and to evalarate the diagnostic value of various skin tests. METHOD: 130 patients with drug eruptions were reviewed clinically. In 54 patients, intradermal test, patch test, and scratch patch test were performed using suspected drugs. Result : The exanthematous eruption(53.0%), fixed drug eruptior.(29.2%), and urticaria(6.2%) were the common types of the drug eruption. The common causative agenst of drug eruptions were antibiotics/antimicrobials, antipyretics/NSAIDs, and CNS depressants. 28 paitients out of the 54 patients on skin tests with suspected drugs had positive results in at least one slinest. CONCLUSION: In our study, we were able to obtain somewhat meaningfi 1 results based on data from the combination of various skin tests.
Central Nervous System Depressants ; Diagnosis ; Drug Eruptions* ; Humans ; Intradermal Tests ; Patch Tests ; Skin Tests* ; Skin*

BACKGROUND: Hydrofluoric acid burns are occuring with ever-increiisir g frequency due to the wide use of this acid in industrial fields, such as electronics and semiconductor i idustries. OBJECTIVE: The purpose of thie study was to evaluate the clinical charateristics of hydrofluoric acid burn. This study will give basic information of hydrofluoric acid burn to the dermatologists who are not familiar with hydrofluoric acid. METHOD: Twenty-two patient who were diagnosed as having hydroiluiiric acid burns at koryo General Hospital(1986-1993) were irraluated clinical)y. Forteen were malc ar d eight were female, most of them were in their third decade. RESULTS: The results are sumrnurised as follows 1. Latent periods were variabie from 30 min. to 3 days. Most patients recognized their chemical burn several hours after exposure. 2. Foot & sole (9 cases) and fingers (7 cases) were most frequently in olved. In 7 cases, nails were also involved. 3. Pain was the most. commori iuobjective symptom. 4. Skin lesions showed erytherna, erythematous swelling, erosion, bulla., necrosis, pigmentation and crust, formation, progressii ely. 5. Treatment Wet dressing with Hyami-solution and topical application of ai tibiotics or steroidointment were sufficient in mild case. In severe cases, intralesional injection of calcium glucoante was perfirmed. Debridement was also performed after the progn ssion of tissue damage stopped. Nails were extracted in cases involving nails. CONCLUSION: Hydrofluoric acid burn is characterized clinically by severc progessive tissue destruction and excruciating pain. Dermatologists need to be aware of the clinical features of hydroclucric acid burn and its proper treatment.
Bandages ; Burns* ; Burns, Chemical ; Calcium ; Debridement ; Female ; Fingers ; Foot ; Humans ; Hydrofluoric Acid* ; Injections, Intralesional ; Necrosis ; Pigmentation ; Semiconductors ; Skin

Recently radiation and radioisotope are being used widely in many fields such as medicine and industry. But only a few papers were reported about the radio dermatitis due to industrial use of radiation. Authors experienced 4 cases of occupational radiodermatitis due to Ir-192 occur red during the radioisotope traspierce test. All 4 patients noticed painful swelling, erythema, and bullae on their fingers or palms 1-2 weeks after exposure. All laboratory tests to detect ystemic effects of radiation were normal. Three patients recovered after treatment with systemic and topical corticosteroid and emollient cream for 2-4 weeks. But one patient suffered from complication of fibrosis and recurrent cellulitis on his fingers. We discussed about the working situation of our patients and reviewed the literatures about the radiodermatitis.
Cellulitis ; Dermatitis ; Erythema ; Fibrosis ; Fingers ; Humans ; Radiodermatitis

We present four cases of herpes zoster treated successfully with vidarabine-5-monophosphate, two patients receiving chernotherapy and radiation therapy due to Hodgkins lymphoma or colon cancer, a patient combined with viral meningitis, and a patient with herpes zoster ophthalmicus. Viclarabine can be used to treat herpes zoster in immunosuppressed patients or patients with malignancy or patients with severe complications.
Colonic Neoplasms ; Herpes Zoster Ophthalmicus ; Herpes Zoster* ; Hodgkin Disease ; Humans ; Meningitis, Viral

A case of Kimuras disease was presented. It seemed that this is the first reported case in Korean literature. The patient was 26 years old male having painless slowly growing tumor-like swelling on his left cheek for 5 months. Mild leukocytosis and marked eosinophilia (27%) were noted in routine laboratory tests. Histopathologically numerous well developed lymphoid follicular structures composed of closely packed lymphocytes and histiocytes were seen in the deep dermis and subcutaneous fat tissue. Dense eosinophilic infiltrations in and around the follicles were peculiar picture. The literature was reviewed. Its relation to the similar descriptions in western literature under various diffe'rent headings was discussed;
Adult ; Cheek ; Dermis ; Eosinophilia ; Eosinophils ; Head ; Histiocytes ; Humans ; Leukocytosis ; Lymphocytes ; Male ; Subcutaneous Fat

Hyperkeratosis of the nipple and areola is a rare skin disordr characterized clinically by thickening and discoloration of the skin of the areola and/or nipple and pathoiog cally by papillomaosis or acanthosis-nigricans like changes. We report a case of hyperkeratosis of the nipple and areola occuring in a woman with no underlying endocrinopathy and no other additional hyperkeratotic conditions. Ttie iresent case seems to be the nevoid form by the Levy-Franckel classification. Treatment with topical medications including steroids and keratolytic agents produced no improvement. Therefore we recommeneded surgical excision because the paient was greatly disturbed by the appearance of the lesions. Surgical excision and full thickness skin graft were performed sucessfully.
Classification ; Female ; Humans ; Keratolytic Agents ; Nipples* ; Skin* ; Steroids ; Transplants*