The present study was to assess the effect of ultrarapid freezing on the development of 1-cell mouse zygote using cryoprotectants, DMSO (dimethyl sulfoxide) or PROH (1,2-propanediol). We investigated the effect of the type and concentration of cryoprotectant, and of the temperature and time of prefreezing equilibration on their capacity to develop to the blastocyst stage in vitro. The concenration, the equilibration temperature, and the exposure time seemed to serve as an important factor in ultrarapid freezing of 1-cell mouse zygotes. In addition to the exposure time and the concentration of cryoprotectant appeared to play a key role in the development of the embryo. In general, the development of the embryo was more effective at 3degrees C than 23degrees C and 4.5 M than 3 M for 3 to 5 minutes. At 23degrees C the development of the embryo was stimulated by DMSO while at 3degrees C it was stimulated by PROH. Thus it has been suggested that there exists a correlation between the concentration of cryoprotectants and exposure time in the development of the embryo. In conclusion, we found that for ultrarapid freezing of mouse 1-cell embryos in DMSO, or PROH-based solution, viability shown optimum depending on the cryoprotectant, the concentration of the cryoprotectant and on the temperature and the duration of equilibration.
Animals ; Blastocyst ; Dimethyl Sulfoxide ; Embryonic Structures ; Freezing* ; Mice* ; Zygote*

Among two groups of navy recruits undergoing training, one consisting of 500 enlisted men and another 100 newly enrolled midshipmen, 18 reported to Chinhae Naval Hospital with fatigue fractures during the period from February though September 1969. Following observations were made on these cases: 1. The incidence of fatigue facture was 1.4 percent among the enlisted men and 11 per cent among the midshipmen. 2. There were 6 cases of complete fraeture and 2 cases of incomplete fracture. In the remaining 10 cases, only periosteal reaction was evdent roentgenographically with no visible fracture line initially: fracture lines, however, became apparent on subsequent examinations in all the cases. 3. The fractures involved the femur in 4 cases and the tibia in 14 cases, a ratio of 1:3. 5 in latter s favor. 4. The site of predirection in the femur was the distal one third of the shaft, occurring in 3 of the cases. In the tibia the junction of the upper and middle thirds was most frequently affected, occurring in 10 of the cases. 5. All the cases in our series healed without any complications.
Fatigue ; Femur ; Fractures, Stress ; Humans ; Incidence ; Male ; Tibia

No abstract available.
Ameloblastoma*

Nowadays, the increased of number the elderly in the of the population is inevitable due to the development of medical science as to the extension of the average span of the human life as a result of the establishment of the public welfare system thru economic growth. Consequently, the number of old aged patients that we anesthesiologists deal with will be on the increase. We should paricularly take greater care in the anesthesia of elderly patients than we do in case of young patients. The decline in cardiopulmonary, renal, hepatic function and endocrine disease will have a great effect on anesthesia. To take account of this diminished physiologic reserve the anesthesiologist should make a routine examination of the problems in applying an anesthesia to the aged patient with regard to the peculiarity of geratric anesthesia. We have analysed 1,372 cases of geristric anesthesia during 1972~1981 at Chonnam National University Hospital. The results were as follow: 1) A total of 19035 anesthesias were done among them, patients of over 60 years were 1,372(7.2%), of which the number of male patients(63.8%) was 1.8 times greater than that of female patient(36.2%). 2) Among the 1,372 cases, 991 cases were 60~69 year old(72.2%), 335 cases were 70~79 year old (24.4%) and 46 cases were 80 year(3.4%). 3) Emergency versus elective surgical cases were 36.5% versus 63.5%. 4) Inhalation anesthesia was performed in 969 cases(70.6%), spinal anesthesia 360 cases(26.3%) and epidural anesthesia numbered 43 cases(3.1%). 5) Halothane anesthesia was markedly increased after 1979 instead of diethyl ether anesthesia. 6) Halothane in inhalation anesthesia, tetracaine in spinal anesthesia and lidocaine in epidural anesthesia were the major anesthetics. 7) In the evaluation of the preoperative condition for 613 elective surgery cases in the last 5years, 171 cases(27.6%) revealed hypertension, 136 cases(22.3%) showed abnormal chest P=A finding, 127 cases(20.7%) showed an abnormal ECG finding and 17 cases (2.8%) revediabetes mellitus. 8) Total mortality rate was 2.1%. The mortality rate of emergency surgery (4.6%) was about 4 times greater than that of elective surgery(0.7%).
Female ; Male ; Humans ; Mortality

Syringoma is not a uncommon tumor of the skin. It is a benign adenoma, of intraepidermal eccrine ducts. It occurs predominantly in women at puberty or adolescence. The lesions usually are multiple and small, skin colored or slightly yellowish payules, usually only 1 or 3mm in size. The lesions usually are limited to the lower eyelids but may occur on cheek, axilla, abdomen, neck, anterior chest and vulva. Rarely these are generalized in distributior. A 23 years old healthy man has had widespread lesions over the face, anterior chest and abdomen. The lesions appeared at the age of 13 on the anterior chest and abdomen. These increased in number and extended slowly to the face On visiting our department, there were numerous, angular or round, skin colored papules on his face and anterior chest as well as yellowish brown colored papules on his abdomen. Diagnosis was confirmed by clinical and histopathological findings as generalized syringoma.
Abdomen ; Adenoma ; Adolescent ; Axilla ; Cheek ; Diagnosis ; Eyelids ; Female ; Humans ; Neck ; Puberty ; Skin ; Syringoma* ; Thorax ; Vulva ; Young Adult

Authors investigated the possible role of intravascular hypercoagulable states on the etiology of Kegg-Clave-Perthes diesease. Forty-five patients with Legg-Clave-Perthes disease(31 avascular stages and 14 reossification stages) and twenty-two normal control patients were subjected to study for evaluation of coagulation and fibrinolysis system by means of the tests which included antiphospholipid antibody(APA), Protein C, Protein S and antithrombin- III (AT- III) for evaluation of coagulation system, and tissue type PIasminogen activator(tPA), Plasminogen activator inhibitor(PAI), D-dimer for fibrinolytic system. APA increased significantly in Legg-Clave-Perthes patients(p=0.016) as compared with control group, while Protein C(p=0.040) and Protein S(P=0.0001) decreased significantly in Legg-Clave- Perthes disease. AT- III increased in Legg-Clave-Perthes disease(p=0.0000). In contrast, there were no statistically significant differences in PAI, tPA, D-dimer between the Legg-Clave-Perthes disease and control group. There were no differences in all parameters between the avascular stage and reossification stage in patients with Legg-Clave-Perthes disease, Suggestive of possible inherent effect in coagulation system(hypercoagulable states) which does not change with time. Based on the above findings authors presumed that hypercoagulable state may contribute to the development of Legg-Calve-Perthes disease. However, to elucidate the etiology of Legg-Calve-Perthes disease, further extensive investigation should be followed, which include the familial tendency of hypercoagulable state, relationship with other multifactorial causes such as alcohol and steroids, and confirmation of intravascular thrombosis or decreased blood perfusion in the femoral head. Also, the significance of abnormally elevated AT-III on the disease should be answered.
Fibrinolysis ; Head ; Humans ; Legg-Calve-Perthes Disease ; Perfusion ; Plasminogen Activators ; Protein C ; Protein S ; Steroids ; Thrombosis

Percutaneous transhepatic biliary drainage has played a major role in treatment of patients with bilairy tractdisease, especially obstruction by malignant disease. Percutaneous transhepatic biliary drainage was performed 128 times in 107 patients of obstructive jaundice for recent 2 years and 8 months from July, 1981 to March, 1984 at departement of radiology, Seol National University Hospital. The resuls were as follows; 1. The male to femaleratio was 2:1 and the age ranged from 19 to 88 with average of 54. 2. The causes of obstructive jaundice included 94 malignant diseases and 13 benign diseases. Malignant diseases were 48 cases of bile duct cancer, 20 cases of metastasis, 20 cases of pancreatic cancer, 4 cases of gallbladder cancer, 1 case of ampulla Vater cancer, and 1case of duodenal cancer. Benign diseases were 8 cases of comon bile duct stone and 3 cases of benign stricture and2 cases of cholangitis. 3. The most common indication was palliative drainage of obstruction secondary to malignant tumor in 78 cases. 4. The overall success rate was 93.7%. Internal drainage was acheved in 34 (26.5%)and external drainage was accomplished 86(67.2%). 5. Decline in serum bilirubin level was found in 100cases(5.6%). Percutaneous transheatpic biliary drainage is a proven technique for non-operative biliarydecompression and established alternative to surgery.
Bile Duct Neoplasms ; Bile Ducts ; Bilirubin ; Cholangitis ; Constriction, Pathologic ; Drainage ; Duodenal Neoplasms ; Gallbladder Neoplasms ; Humans ; Jaundice, Obstructive ; Male ; Neoplasm Metastasis ; Pancreatic Neoplasms

Over a period of recent two years, 100 patients who had localized pulmonary lesions and underwent percutaneousneedle aspiration and biopsy, were analized. There were 56 malignancies and 44 benign and 79% respectively.Differentiation of malignancy vs. benignity was possible in 89% of cases obviating unnecessary exploratorythoracotomy for diagnostic purpose, Five cases were misinterpreted and eight cases were non-diagnostic oncytology. Inadequate materail was obtained in two cases; one was due to hardness of the mass, which, later,confirmed as chondrosarcoma, and the other was too samll(0.8x1.0cm) to be visible on lateral view. Theobainenability of the tissue was 98%. 14(14%) patients developed pneumothorax; one of them required treatment andthe reminder showed spontaneous resporption. (Transient neglibigle blood tinged sputum was found in 16(16%)cases.) The method, problems and complications are discussed. Authors recommend the percutaneous needle aspirationand biopsy as the initial procedure in diagnostic work-up of pulmonary coin lesions, especially when they aresmaller, more peripheral and metastatic neoplasm is neoplasm is suspected.
Biopsy ; Biopsy, Needle ; Chondrosarcoma ; Hardness ; Humans ; Methods ; Needles ; Pneumothorax ; Solitary Pulmonary Nodule ; Sputum ; Tolnaftate

The etiologic factors causing low back pain are numerous and variable. Orthopaedic sugreons tend to focus attention to any defects of structural and functional origins such as pine, discs and facet joints. Clinically, however, the fact remains that not all abnormal findings produce back pain and vice vera. Among the many modalities of investigation to determine the etiology of back pain, plain X-ray of the lumbosacral spine is the single most important acreening method. In addition to antero-posterior, lateral and oblique views, stress view of the spine gives certain clue to predict the disc degeneration. The X-ray findings of a total of 503 patients with low back pain (the study group). admitted during the year of 1977 to the Department of Orthopaedic Surgery, National Medical Center, were reviewed and compared with the control group of 100 patients without relevance to back pain (the control group). Our intention was to explore the relationship between the abnormal findings of plain X-rays and occurence of back pain. and to further entrance the diagnostic value of the spine. The study group of 503 patients (86 cases of which were operated on) were diagnosed not only by routine plain X-rays but also by contrast myelography and tomography in addition to detailed physical examination. Following observations were made: 1. The sex was nearly equally distributed in the atudy group of 503 patients. The age ranged from the second to the eighth decade, dominant group being the third to fifth decades. 2. The causes of the low back pain were herniated disc, spondylosis, spondylolysis, and infection, in that order. 119 patients (23.7%) out of the 503 showed no roentgenologicaI abnormal findings. 3. 384 cases out of the 503 revealed abnormal findings, mainly congenital or developmental and degenerative changes in that order. 4. In a comparative study with the control group, congenital or developmental anomalies and degenerative changes were not related to low back pain. Disc. space narrowing, supposed to be valuable in establishing the diagnosis of low back pain was present in 26% of the cases in study group and in 11% in the control group. 5. The age incidence of the low back pain with congenital or developmental anomalies was mainly in th second and third decades, while that of disc space narrowing was in the third to fifth decade. The degenerative changes of the spine appeared after the fourth decade. 6. Transitional vertebra and tropism are supposed to plsy a role on the degenerative change of the spine. 7. The stress study of the spine had some benifit it the diagnosis of disc degeneration. The mobility of the L4-5 lesion with low back pain was increased to mean 15.1 and standard deviation 3.7, in comparison to the mean 12.3 and standard deviation 3.7 in the control group.
Back Pain ; Diagnosis ; Humans ; Incidence ; Intention ; Intervertebral Disc Degeneration ; Intervertebral Disc Displacement ; Low Back Pain ; Methods ; Myelography ; Physical Examination ; Spine ; Spondylolysis ; Spondylosis ; Tropism ; Zygapophyseal Joint

A 69-year-old man was admitted to the hospital because of flu-like symptoms and fatigue for 2 weeks. Computed tomography revealed ground glass opacity and consolidation in both the lungs as well as pleural effusion. The patient was diagnosed with pneumonia and was hospitalized. At the time of hospitalization, he complained of shortness of breath and coughed-up blood-tinged sputum. Two days after admission, he died suddenly. An autopsy was performed; cardiomegaly was noted, and further examination revealed that the aortic valve had been destroyed by multiple, irregular vegetations. Herein, we report an autopsy case of infective endocarditis with a review of the relevant literatures.
Aged ; Aortic Valve ; Autopsy* ; Cardiomegaly ; Dyspnea ; Endocarditis* ; Fatigue ; Glass ; Hospitalization ; Humans ; Lung ; Pleural Effusion ; Pneumonia ; Sputum