1.Immunoblot findings of calcareous corpuscles binding proteins in cyst fluid of Taenia solium metacestodes.
The Korean Journal of Parasitology 2004;42(3):141-143
After collecting calcareous corpuscles from plerocercoid of Spirometra mansoni (sparganum), we evaluated the antigenic values of calcareous corpuscles binding proteins obtained from the cyst fluid of Taenia solium metacestodes. Immunoblot analysis revealed that cysticercosis patient sera strongly recognized 10 and 95 kDa calcareous corpuscles binding proteins. This result demonstrated that calcareous corpuscles are bound with major secretory antigenic proteins, which is possibly involved in the secretory pathways of the 10 and 95 kDa proteins presenting in the cyst fluid of T. solium metacestodes.
Animals
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Antigens, Helminth/*analysis
;
Carrier Proteins/*immunology
;
Cysticercosis/diagnosis/immunology
;
Helminth Proteins/*immunology
;
Humans
;
Immunoblotting/methods
;
Molecular Weight
;
Serologic Tests
;
Sparganum
;
Taenia solium/*chemistry/immunology
2.Modification of carbohydrate compositions of 31/36 kDa proteins of plerocercoids (sparganum) of Spirometra mansoni grown in different intermediate hosts.
The Korean Journal of Parasitology 2004;42(2):77-79
We purified specific 31/36 kDa antigenic molecules from sparganum in different intermediate hosts (snakes and mice) and analyzed their monosaccharide compositions. Compositional analysis showed that glucose and mannose concentrations were 2-3 fold higher in the 31/36 kDa molecule purified from snakes than those from mice. This result implies that antigenic glycoproteins of sparganum from snakes might be modified in mammalian sparganosis with respect to their carbohydrate composition.
Animals
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Antigens, Helminth/*chemistry
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Human
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Mice
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Monosaccharides/*analysis
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Snakes/parasitology
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Sparganum/immunology
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Spirometra/*immunology
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Support, Non-U.S. Gov't
3.Abdominal Distension.
Journal of the Korean Medical Association 2001;44(4):427-432
No abstract available.
4.The Last Fifty Years of Western Medicine in Korea: Korean Surgical Society.
Journal of the Korean Medical Association 1997;40(8):986-989
No abstract available.
Korea*
5.Significance of Sentinel Node Biopsy in Cancer.
Journal of the Korean Medical Association 1998;41(3):336-339
No abstract available.
Biopsy*
7.Correction of Malalignment during Subtrochanteric Nailing.
Journal of the Korean Fracture Society 2009;22(1):66-70
No abstract available.
Nails
8.Multiple Myeloma Combined with Stomach Cancer: A case report.
Chang Heon YANG ; Myung Soo HYUN ; Hyun Woo LEE
Yeungnam University Journal of Medicine 1989;6(1):197-204
A case of multiple myeloma combined with stomach cancer and related literatures were reviewed. A 67 year-old male patient entered the hospital with dysphagia and weight loss for 3 months. Peripheral blood examination revealed anemia with rouleaux formation. Total protein of the serum was 9.9 g/dl with hyperglobulinemia (albumin 2.7 g/dl, globulin 7.2 g/dl, A/G ratio 0.375). On the electrophoresis and immunoelectrophoresis of the serum, the abnormal protein with the pattern of monoclonal gammopathy (IgG-K type) was shown. There were multiple variable sized osteolytic lesions on skull X-ray and abnormal hot uptakes of rips on bone scan and result of rib biopsy was plasmacytoma. Gastrofiberscopy was performed to search for the cause of upper gastrointestinal bleeding, revealed stomach cancer, and the result of the gastric mucosal biopsy proved to be well-differentiated adenocarcinoma.
Adenocarcinoma
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Anemia
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Biopsy
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Deglutition Disorders
;
Electrophoresis
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Hemorrhage
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Humans
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Immunoelectrophoresis
;
Male
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Multiple Myeloma*
;
Paraproteinemias
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Plasmacytoma
;
Ribs
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Skull
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Stomach Neoplasms*
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Stomach*
;
Weight Loss
9.Bilateral breast cancer.
Jung Hyun YANG ; Hyun Chang KIM ; Ze Hong WOO
Journal of the Korean Surgical Society 1993;44(6):961-968
No abstract available.
Breast Neoplasms*
;
Breast*
10.Immunolocalization of the 150 kDa protein in cyst fluid of Taenia solium metacestodes.
Hyun Jong YANG ; Young Bae CHUNG
The Korean Journal of Parasitology 2004;42(2):81-84
The 150 kDa protein of cyst fluid (CF) of Taenia solium metacestodes was purified by ammonium sulfate fractionation and Superose 6 HR gel filtration chromatography. The purified protein consisted of three subunits (15, 10 and 7 kDa proteins), which were analyzed with the use of a 7.5-15% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Immunofluorescence study was carried out by using immunize specific polyclonal antibody. Positive reactions were noticed at bladder walls, calcareous corpuscles, granules of cyst fluid and some host tissue surrounding the bladder wall of the metacestodes. These results suggest that the 150 kDa protein was secreted into host tissues, inducing immune responses in the host, and it may play important roles in the cellular physiology of the parasites.
Animals
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Chemical Fractionation
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Chromatography, Gel
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Cyst Fluid/*chemistry/metabolism/parasitology
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Cysticercosis/*metabolism/parasitology
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Electrophoresis, Polyacrylamide Gel
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Helminth Proteins/chemistry/*isolation & purification
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Mice
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Mice, Inbred BALB C
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Microscopy, Fluorescence
;
Molecular Weight
;
Support, Non-U.S. Gov't
;
Swine
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Swine Diseases/parasitology
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Taenia solium/*metabolism