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1.Cortisol and catecholamine in internal spermatic vein: are they toxic materials responsible for impairment of the spermatogenesis in varicocele?.

The crystalline surface layer protein (SLP) and a 28-32 kDa antigen of Rickettsia typhi were known as strong immunogens. We previously reported a cloning and sequence analysis of the SLP gene of R. typhi (slpT) and showed that the open reading frame of this gene encodes both the SLP and a 32-kDa protein. Our study also showed that a 48-kDa protein reacted strongly with polyclonal antiserum of a patient with murine typhus. In this study, we produced three recombinant proteins (SLP, 32-kDa, and 48-kDa protein) in E. coli as fusion proteins with maltose binding proteins. The reactivity of these proteins with patients' sera was investigated.
Clone Cells* ; Cloning, Organism* ; Crystallins ; Humans ; Maltose-Binding Proteins ; Open Reading Frames ; Recombinant Proteins ; Rickettsia typhi* ; Rickettsia* ; Sequence Analysis ; Typhus, Endemic Flea-Borne

5.Correlation study on foods/nutrients intake and the mortality and the morbidity of carcers of stomach and colorectum in Korea.

Myong Kyun CYNN ; Dong Hyun KIM ; Keun Young YOO

Korean Journal of Epidemiology 1993;15(2):119-131

6.Classification of Borrelia burgdorferi Sensu Lato Isolated in Korea by Restriction Fragment Length Polymorphism.

Kyung Hee PARK ; Myong Joon HAHN ; Seung Hyun LEE ; Jong Hyun KIM

Journal of the Korean Society for Microbiology 1998;33(2):119-128

The organization of the ribosomal genes is unique in Borrelia burgdorferi in that the rrl (23S) and rrf (SS) genes are duplicated in tandem. Twelve Korean Borrelia isolates were classified by the method of rRNA gene restriction fragment length polymorphism (RFLP) and Southern hybridization. One of the isolates, KW-3, as well as the representative of ribotype group IV Ip89 appeared to have evolved within B. garinii. The size of the restriction bands of the 11 isolates showed different patterns from the USA and European isolates. The result by RFLP of rrf-rrl intergenic spacer region amplified by PCR showed a good correlation with that of Southern hybridization of rRNA gene. Therefore, the eleven Korean isolates could be classified as members of a new Borrelia group.
Borrelia burgdorferi Group* ; Borrelia burgdorferi* ; Borrelia* ; Classification* ; Genes, rRNA ; Korea* ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length* ; Ribotyping

7.Significance of IgG and IgM antibodies in the diagnosis of scrub typhus and evaluation of rickettsia tsutsugamushi strain Boryong as a diagnostic antigen.

Woo Hyun CHANG ; Sun Ho KEE ; Mu Jin CHU ; Myong Sik CHOI ; Ik Sang KIM

Journal of the Korean Society for Microbiology 1992;27(1):19-27

8.Significance of IgG and IgM antibodies in the diagnosis of scrub typhus and evaluation of rickettsia tsutsugamushi strain Boryong as a diagnostic antigen.

Woo Hyun CHANG ; Sun Ho KEE ; Mu Jin CHU ; Myong Sik CHOI ; Ik Sang KIM

Journal of the Korean Society for Microbiology 1992;27(1):19-27

9.Broken Suture after Keratoplasty.

Hyun Seung KIM ; Yoon Won MYONG

Journal of the Korean Ophthalmological Society 1996;37(2):219-224

We performed keratoplasty and did not remove suture material in 189 patients (189 eyes) after operation. Among them, 48 eyes which had clear grafts with spontaneous suture breakage had been analyzed retrospectively for the age, the sex distribution, the time and the site of broken suture, the preoperative diagnosis and the relationship between the suture breakage and the presence of vasculized recipient bed or suture method. The time when suture was broken after keratoplasty ranged from 3 months to 80 months(average 30 months). Leukoma cornea, 25 eyes(52.1%) and keratoconus, 13 eyes(27.1%) were the most common cause of keratoplasty and the others were corneal dystrophy, Fuchs dystrophy and re-keratoplasty. The most common site of broken suture was at superior quadrant(30 eyes, 62.5%), The vasculized recipient bed was found in 16 eyes, 13 eyes of leukoma cornea and 3 eyes of fe-keratoplasty in preoperative state. The statistical analysis showed significant relationship between the presence of vasculized recipient bed and the suture breakage(p<0.05). Suture was broken in 28 eyes(26.7%) of 105 eyes with single continuous suture, 6 eyes(21.9%) of 20 eyes with combined interupted suture and 14 eyes(21.9%) of 64 eyes with combined interupted and continuous suture. The statistical analysis showed no significant relationship between the suture method and the suture breakage(p>0.05).
Cornea ; Corneal Opacity ; Corneal Transplantation* ; Diagnosis ; Fuchs' Endothelial Dystrophy ; Humans ; Keratoconus ; Retrospective Studies ; Sex Distribution ; Sutures* ; Transplants

10.Microwave stimulated staining of bone marrow aspiration smears and plastic embedded section.

Jong Hyun YOON ; Myong Ok SHIM ; Woon Heung SONG ; Han Ik CHO ; Sang In KIM

Korean Journal of Hematology 1991;26(2):357-362

1.Cortisol and catecholamine in internal spermatic vein: are they toxic materials responsible for impairment of the spermatogenesis in varicocele?.

2.Monoclonal antibodies specific to rickettsia typhi.

3.Monoclonal antibodies specific to rickettsia typhi.

4.Cloning and Expression of the Gene Encoding the 32-kDa Protein of Rickettsia typhi.

5.Correlation study on foods/nutrients intake and the mortality and the morbidity of carcers of stomach and colorectum in Korea.

6.Classification of Borrelia burgdorferi Sensu Lato Isolated in Korea by Restriction Fragment Length Polymorphism.

7.Significance of IgG and IgM antibodies in the diagnosis of scrub typhus and evaluation of rickettsia tsutsugamushi strain Boryong as a diagnostic antigen.

8.Significance of IgG and IgM antibodies in the diagnosis of scrub typhus and evaluation of rickettsia tsutsugamushi strain Boryong as a diagnostic antigen.

9.Broken Suture after Keratoplasty.

10.Microwave stimulated staining of bone marrow aspiration smears and plastic embedded section.

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