BACKGROUND: Isolated lung perfusion (ILP) was developed as a new treatment approach to non-resectable primary or metastatic lung cancer, because of its ability to reduce systemic toxicity while delivering high-dose chemotherapeutic agents to the target organs. This research was planned to evaluate the direct toxic effect of high-dose cisplatin to the lung tissue during isolated lung perfusion. MATERIAL AND METHOD: Fifteen mongrel dogs were divided in the perfusate for 40 minutes. The second group was composed of 5 mongrel dogs which underwent ILP with cisplatin 2.5 mg/Kg added to the perfusate for 30 minutes and 10 minutes with washing solution without cisplatin. The third group underwent the same procedure as the second group except cisplatin 5.0 mg/Kg in the perfusate. Activities of serum angiotensin converting enzyme (ACE), tumor necrosis factor-alpha (TNF-alpha), and concentration of serum lactate dehydrogenase (LDH) and blood urea nitrogen/creatinine (BUN/Cr) were analyzed in each groups at the time of pre-perfusion, 1 hour, 1 day, 1 week, and 2 weeks after ILP. RESULT: Serum ACE activities before and 1 hour, 1 day, 1 week, and 2 weeks after ILP in control group were 45.1+/-6.3, 44.6+/-9.3, 46.7+/-9.5, 50.8+/-9.1, 46.1+/-4.3 U/L. Those in cisplatin 2.5 and 5.0 mg/Kg groups were 49.4+/-12.6, 39.0+/-8.6, 42.3+/-15.9, 50.0+/-2.6, 53.8+/-8.3 and 55.5+/-12.3, 47.0+/-6.3, 45.1+/-6.9, 74.8+/-19.5, 60.2+/-12.0 U/L, respectively. Serum TNF-alpha activities in each group before and after ILP were 5.0+/-1.5 / 7.7+/-2.2 / 6.6+/-2.5 / 4.3+/-1.3 / 5.2+/-1.1 (control), 8.7+/-1.6 / 9.9+/-2.2 / 7.9+/-1.5 / 6.3+/-2.2 / 7.4+/-2.4 (cisplatin 2.5 mg/Kg), and 6.9+/-0.7 / 8.9+/-3.4 / 7.9+/-4.0 / 3.3+/-0.9 / 5.8+/-1.3 pg/ml (cisplatin 5.0 mg/Kg). Mean LDH levels of each group were 225.7 / 271.3 / 328.9 / 350.8 / 255.7(control), 235.7 / 265.7 / 336.0 / 379.5 / 299.2 (cisplatin 2.5 mg/Kg), and 259.6 / 285.2 / 340.6 / 433.4 / 292.4 IU/L (cisplatin 5.0 mg/Kg). So there was no significant difference in serum ACE, TNF-alpha, and LDH activity changes after ILP between the 3 groups. And, there was no significant changes in BUN/Cr in each groups, which was independent of ILP and perfused concentration of cisplatin. In addition, all dogs survived the ILP and there was no significant evidence of pulmonary vascular injury after 2 weeks of ILP with cisplatin. CONCLUSION: There was no harmful effect of cisplatin to the lund tissue of the mongrel dog up to 5.0 mg/Kg in perfusate. Therefore, it is perceived to be safe and effective to deliver high-dose cisplatin to the lung without pulmonary toxicity and renal damage with ILP.
Animals ; Cisplatin* ; Dogs* ; L-Lactate Dehydrogenase ; Lung Neoplasms ; Lung* ; Peptidyl-Dipeptidase A ; Perfusion* ; Tumor Necrosis Factor-alpha ; Urea ; Vascular System Injuries

Adrenal gland is a rare location for an oncocytic neoplasm. In English literature less than 10 cases of adrenocortical oncocytoma have been reported. We have experienced a case of adrenocortical oncocytoma in a 35-year-old man which was detected incidentally during the ultra-sonographic evaluation of the abdomen for a routine physical examination. This case did not demonstrate any clinical evidence of adrenocortical abnomalities, such as virilization or hypertension. Grossly, the tumor was light to dark tan on cut surface. Light-microscopic examination revealed tumor cells with abundant lipid- sparse eosinophilic cytoplasm and occasional pleomorphic nuclei. Mitotic figures were less than 5/50 HPFs. Tumor cells were positive for vimentin but negative for pancytokeratin, CAM 5.2, chromogranin and synaptophysin. Ultrastructural examination demonstrated abundant mitochondria containing occasional intramitochondrial dense bodies or inclusions.
Abdomen ; Adenoma, Oxyphilic* ; Adrenal Glands ; Adult ; Cytoplasm ; Eosinophils ; Humans ; Hypertension ; Mitochondria ; Physical Examination ; Synaptophysin ; Triacetoneamine-N-Oxyl ; Vimentin ; Virilism

Purpose: The purpose of this study was to explore patients’ awareness and treatment experiences with Chronic Obstructive Pulmonary Disease (COPD) through in-depth interviews. The objective was to develop data to support patients in the successful management of their illness. Methods: Purposeful sampling was used to collect information- rich cases and participants were recruited at a university hospital in Daegu, South Korea. Data collection was done through face to face in-depth individual interviews with 10 patients with COPD from November 2016 to December 2018. Interview began with open-ended questions and each interview lasted on average one hour. All interviews were audio-taped and transcribed verbatim and transcripts were analyzed using qualitative thematic analysis. The findings were verified by the participants. Results: Four themes emerged from data analysis: ‘Feeling like the disease is not my disease,’ ‘Terminology spills like a flood,’ ‘Negligent daily disease-management with relief,’ and ‘Relying on health professionals and family members hoping for healing’. Conclusion Participants do not have a clear understanding of their illness nor feel that they are principal agents of their health and illness for treatment and disease management; instead, they depend mainly on their family members and doctors. The findings elucidated the need for increasing patients’ awareness of the illness. Nurses should develop educational support programs to help COPD patients improve their awareness of their illness and successfully manage their treatment.

No abstract available.
Fertilization in Vitro* ; Gonadotropin-Releasing Hormone*

PURPOSE: This research is to understand the effects of senior simulation on employees of elderly care facilities and utilize it in their job training. METHODS: This research is a nonequivalence control group pre-to-post quasiexperiment research. 18 employees who have experienced senior simulation are set as test group and 18 other employees who have not experienced senior simulation are set as control group. RESULTS: The hypothesis that 'the test group which has experienced senior simulation will show a positive change in their attitude on elderly care, compared to the control group' and 'the test group which has experienced senior simulation will show a positive change in understanding of elders, compared to the control group' was supported. The hypothesis that 'the test group which has experienced senior simulation will show a positive change in job satisfaction and performance of duties, compared to the control group' was dismissed. CONCLUSION: The senior simulation seems to contribute to elderly care facilities employees' change in their viewpoints of elders and broaden their understanding of them. The senior simulation was meaningful to provide basic help with implementing job training programs.
Aged ; Humans ; Job Satisfaction ; Nursing Homes

PURPOSE:Ghrelin is an endogenous ligand of the growth hormone secretagogue receptor, and stomach is the major site of ghrelin secretion. The purpose of this study is to compare the serum ghrelin concentrations between patients with type 2 diabetes mellitus (DM) and normal adults. We studied also whether serum ghrelin levels in the patients with type 2 DM are correlated with body mass index (BMI), serum insulin, lipid profiles, and creatinine levels. METHODS:Forty patients with type 2 DM and forty normal adults were included in this study. We measured heights and weights of the subjects and calculated their BMIs. Blood samples were obtained to measure the ghrelin concentration and their sera were stored at -20degreeC until used. In all subjects, serum ghrelin levels were measured using the commercially available Ghrelin(human) EIA kit. RESULTS:No differences of mean values were detected between the control group and the type 2 diabetic group for age, body weight, BMI, and the levels of serum total cholesterol, triglyceride, HDL cholesterol, and creatinine. But ghrelin level of the type 2 diabetic group (71.1+/-30.5 ng/L) was significantly lower than the control group (139.7+/-36.9 ng/L). In the control group, the ghrelin level showed positive correlation with HDL cholesterol (Pearson's correlation coefficient=0.37, P<0.05). In the diabetic group, the ghrelin level showed weakly positive correlation with insulin concentration. However, there was no significant relationship between serum ghrelin and various parameters in the diabetic patients group. CONCLUSION: In this study, ghrelin concentration in type 2 diabetic patients was lower than that in the control group. In the control group, serum ghrelin concentrations were positively correlated with HDL cholesterol. In the type 2 diabetic group, there was no significant correlation between insulin and ghrelin concentrations.
Adult ; Body Mass Index ; Body Weight ; Cholesterol ; Cholesterol, HDL ; Creatinine ; Diabetes Mellitus ; Diabetes Mellitus, Type 2* ; Ghrelin* ; Humans ; Insulin ; Receptors, Ghrelin ; Stomach ; Triglycerides ; Weights and Measures

No abstract available.
Erythema*

The health-related habits dietary behaviors and nutrient intakes of the elderly living in Cheongju were investigated by interviews with a questionnaire from August to September in 1996. The responses of 169 free-living elderly aged over 65(male 91, female 78) were analyzed. Thirty-six percent of the subjects smoked currently, 33%, drank alcoholic beverages, and 52% exercise regularly. Exercise was mostly in the from of walking for 1/2-1 hour. Significantly higher proportions of males smoked, drank, and exercised as compared to females. Food groups eaten at every meal were examined on a 5-point scale, and the consumption scores were compared with groups according to sex, age, smoking, drinking, and exercise habits. The elderly who exercised regularly got significantly higher scores in their calcium intakes and the variety of the 6 food groups than the elderly who did not exercise. The dietary assessment data showed that the energy intakes of males and females were 81.9% and 72.8% of the RDAs, respectively. The proportions of the elderly consuming less than 75% of RDAs of vitamin A and calcium were 96.5% and 91.1% of the total subjects, respectively. Higher educational status, more pocket money and regular exercise had positive effects on nutrient intakes, while smoking and drinking among females, but not males, had negative effects. Therefore, to improve nutritional status of the elderly, intake of vitamin A-and calcium-rich food such as milk with regular exercise should be emphasized on the education program.
Aged* ; Alcohol Drinking ; Alcoholic Beverages ; Calcium ; Chungcheongbuk-do* ; Drinking ; Education ; Educational Status ; Female ; Food Habits* ; Humans ; Male ; Meals ; Milk ; Nutritional Status* ; Smoke ; Smoking ; Vitamin A ; Vitamins ; Walking ; Surveys and Questionnaires

This study examined the effects of excess intake of calcium(Ca) and iron(Fe) supplements on iron bioavailability, liver and kidney functions in anemic model rats. Seven-week-old female rats were first fed and Fe-deficient diet for ten weeks, and then fed one of nine experimental diets for an additional eight weeks, containing three levels of Ca, normal (0.5%) or high(1.5%) or excess (2.5%) and three levels of Fe, normal(35ppm) or high(210 ppm) or excess(350ppm). In anemic model rats, serum Fe, total iron binding capacity(TIBC), hemogolin(Hb), hematocrit(Hct) and liver Fe contents were significantly decreased. Apparent Fe absorption significantly increased with increasing dietary Fe levels, and decreased with increasing dietary Ca levels. serum Fe concentration significantly increased in rats fed a high- and excess-Fe diet, and decreased in rats fed a excess-Ca diet. TIBC was decreawed in rats fed a excess-Ca diet, and transferrin saturation(%) increased in rats fed ahigh- and excess-Fe diet. Hb and Hct were decreased in rats fed an excess-Ca diet regardless of dietary Fe levels. Fe and thiobarbituric acid reactin gsubstance(TBARS) Contents of liver significantly increased in rats fed a high- and excess0-Fe diet, and decreased in rats fed a high- and excess-Ca diet. Fe content of the spleen showed similar results. Urinary creatinine and GFR increased in rats fed an excess-Ca diet regardless of dietary Fe levels. GOT, GPT and LDH were not significantly affected by dietary Ca and Fe levels. These results suggest that excess intake of Fe may increase liver Fe deposits and TBARS, and excess intake of Ca may decrease Fe bioavailability and kidney function leading to potential health problems in anemic model rats.
Absorption ; Animals ; Biological Availability* ; Calcium* ; Creatinine ; Diet ; Female ; Humans ; Iron* ; Kidney* ; Liver* ; Rats* ; Spleen ; Thiobarbituric Acid Reactive Substances ; Transferrin

This study was performed to investigate body image perceptions of women about themselves and their preschool children and also to investigate the relationship between these perceptions. Subjects were 545 women and their children (279 boys and 268 girls) residing in Daejeon city. 67.7% of women were classified as normal group, 18.6% as overweight group, and 13.8% as underweight group by BMI. 68.5% and 78.7% of their boys and girls, respectively, were classified as normal group, 22.4% and 16.4% as overweight group, 9.1% and 4.9% as underweight group by WLI. Women made relatively accurate judgments on their current body sizes and selected their body images as the most desirable one. But they preferred plumper figures for their children and failed to perceive their overweight children as overweight. While 74.7% of women wished to be thinner, 81.1% of them wished their children to be fatter. Women spending less then 100 thousand won and more than 500 thousand won as monthly food expenses and having only one child perceived their children's current body sizes the lowest and the highest, respectively. Women wished boys to be fatter than girls. Their current body sizes were correlated positively with the children's current body size (p<.01), and their healthiest, attractive, and 'wish' figures were correlated positively with children's current, healthiest, attractive, and 'wish' figures (p<.05 - p<.01). Body size dissatisfaction (wish to be thinner) and BMI of women were correlated negatively with children's healthiest and attractive figures (p<.01). These findings suggest that in order to correct the women's body image misperceptions and to prevent childhood obesity, mother's perception about healthy body images for themselves and their children need to be included in nutrition education. Also, it is necessary to assist mothers to understand the relationship of body size and weight status with the risk of chronic disease which might appear later in their children's life.
Body Image* ; Body Size ; Child ; Child, Preschool* ; Chronic Disease ; Education ; Female ; Humans ; Judgment ; Mothers ; Overweight ; Pediatric Obesity ; Thinness