1.Gene rearrangement of specific exons of immunoglobulin heavy chain joining region in B- lymphoid leukemias.
Chan Jeoung PARK ; Hyun Chan CHO ; John UHMACHER
Korean Journal of Hematology 1993;28(2):307-312
No abstract available.
Exons*
;
Gene Rearrangement*
;
Immunoglobulin Heavy Chains*
;
Immunoglobulins*
;
Leukemia, Lymphoid*
2.Organotypic Culture of HaCaT cells: Use of Dermal Substrate that Combines de-epidermized Dermis with Fibroblast-populated Collagen Matrix.
Kwang Hyun CHO ; Dong Youn LEE ; Kyoung Chan PARK ; Kwang Hyun CHO
Annals of Dermatology 2002;14(3):137-142
BACKGROUND: The immortalized human keratinocyte line, HaCaT cells have been widely used as substitutes for normal epidermal keratinocytes. Recently, reconstruction of a skin equivalent using HaCaT cells showed a multilayered epithelium,but somewhat different tissue architecture as compared with normal epidermis. OBJECTIVE: In this study, using HaCaT cells we tried to reconstruct an epidermis resembling more closely to normal epidermis than the previous results. MATERIALS AND METHODS: HaCaT cells were cultured in air-liquid interface on a recently developed dermal substrated in our laboratory, de-epidermized dermis (DED) raised on fibroblast-populated collagen matrix and the result was compared with those on DED or fibroblast-populated collagen matrix alone. RESULTS: HaCaT cells on the new dermal substrate formed a multilayered epithelium with rete ridges, showing rather orderly cellular organization compared with those on fibroblast-populated collagen matrix. However, horny and granular layers were not observed contrary to normal epidermis. Immunohistochemical studies revealed that differentiation markers such as keratin 1, keratin 6 and involucrin showed the similar pattern to those in HaCaT cells cultured on fibroblast-populated collagen matrix. Markers of terminal differentiation, loricrin and filaggrin were not expressed contrary to normal epidermis. CONCLUSION: These results suggest that organotypic culture HaCaT cells on the dermal substrate combines DED with fivroblast-populated collagen matrix results in incomplete differentiation of HaCaT cells contrary to normal keratinocytes.
Antigens, Differentiation
;
Collagen*
;
Dermis*
;
Epidermis
;
Epithelium
;
Humans
;
Keratin-1
;
Keratin-6
;
Keratinocytes
;
Skin
3.A Case of Diffuse Biphasic Cutaneous Amyloidosis.
Young Min PARK ; Koo Seog CHAE ; Sang Hyun CHO ; Baik Kee CHO ; Chan Kum PARK
Annals of Dermatology 1997;9(4):281-285
We described a case of diffuse biphasic cutaneous amyloidosis, a unique form of localized cutaneous amyloidosis. A 41-year-old man has gradually developed a lichenoid papular and a grouped spotted pigmented macular eruption on the trunk and upper extremities over the past 15 years. Histopathologic examination revealed that amyloid deposits were present in the papillary dermis. It was confirmed by Congo red staining, immunohistochemistry and electron microscopy. There was no evidence of systemic amyloidosis.
Adult
;
Amyloidosis*
;
Congo Red
;
Dermis
;
Humans
;
Immunohistochemistry
;
Microscopy, Electron
;
Plaque, Amyloid
;
Upper Extremity
4.Clinical application of diagnosis laparoscopy in gynecology.
Woo Hyun JEONG ; Tai Ho CHUNG ; Jung Hyun CHO ; Yoon Ho LEE ; Dong Jei CHO ; Chan Ho SONG
Korean Journal of Obstetrics and Gynecology 1991;34(9):1302-1312
No abstract available.
Diagnosis*
;
Gynecology*
;
Laparoscopy*
5.Cutaneous Metastasis from Internal Malignancy.
You Chan KIM ; Kwang Hyun CHO ; Yoo Shin LEE ; Eui Keun HAM
Korean Journal of Dermatology 1987;25(2):213-221
We reviewed clinical records and histologic materials of 96 cases of metastatic skin cancer in Seoul National University Hospital from January 1973 to July 1986. The metastatic skin cancers of male were twice as many as that of female and they occurred most frequently in male at their fifties, female, forties. The most frequent primary cancers in men were carcinoma of the stomach, lung, colorectum and pancreas; in women, carcinoma of the breast, stomach, lung and uterine cervix. Cutaneous metastases from the carcinomas of the liver, uterine cervix and stomach which were many in Korea were smaller in number than that from the carcinomas of the lung and breast. Metastatic lesions were recognized before the primary tumor relatively often in carcinoma of the lung, pancreas and rarely in carcinoma of the uterine cervix, stomach. Though localizations of cutaneous metasta.sis were widespread over all body surfaces, regional areas of primary tumor were predisposed to metastasis. Metastatic carcinoma usually produced nonspecific nodules in the skin. Less commonly, some lesions of metastasis showed inflammatory or sclerotic plaque. The histologic findinga of cutaneous metastases generally mimicked that of primary tumors; nevertheless, in most instances, it was not possible to recognize the original tumor from a histologic examination of metastatic skin cancer and it was possible to classify the metastatic carcinoma only as an adenocarcinoma, a squamous cell carcinoma or an undifferntiated carcinoma.
Adenocarcinoma
;
Breast
;
Carcinoma, Squamous Cell
;
Cervix Uteri
;
Female
;
Humans
;
Korea
;
Liver
;
Lung
;
Male
;
Neoplasm Metastasis*
;
Pancreas
;
Seoul
;
Skin
;
Skin Neoplasms
;
Stomach
6.A Case of Persistent Grover's Disease.
Ki Ho KIM ; Yoo Chan KIM ; Ai Young LEE ; Kwang Hyun CHO ; Yoo Shin LEE
Korean Journal of Dermatology 1989;27(5):582-588
We herein presented a case of persistent variant of Grovers disease in a 83-year old male. He had a rather extensive eruption of erythematous scaly papules, which had been profusely grouped but discretely distributed over 2 years or more. The histopathologic findings of two lesions in the same patient revealed mixture of various histologic patterns, patterns resembling Dariers disease, pemphigus vulgaris, Hailey-Hailey disease and spongiosis. The ultrastructural findings of Darier pattern should be essentially similar to those of Dariers disease and also be common to those observed in the early stages of other causes of focal acantholytic dyskeratosis. The patient had been treated with topical and intralesional steroid and oral 13-cis-isotretinoin, however, the lesions had been persisted only with partial improvement of clinical appearance and reduction of pruritus.
Aged, 80 and over
;
Darier Disease
;
Humans
;
Male
;
Pemphigus
;
Pemphigus, Benign Familial
;
Pruritus
7.Transcervical myomectomy with resectoscopy.
Won Il PARK ; Ki Hyun PARK ; Byung Suk LEE ; Dong Jae CHO ; Chan Ho SONG
Korean Journal of Fertility and Sterility 1993;20(3):301-305
No abstract available.
8.An Analysis of The Patients with Non-Traumatic Chest Pain Patients in Emergency Department according to Goldman's Algorithm.
Suk Jin CHO ; Jin Hyun YOO ; Chan Woong KIM ; Eun Kyung EO ; Koo Young JUNG
Journal of the Korean Society of Emergency Medicine 1999;10(4):599-606
BACKGROUND: It is important to evaluate the patient presenting with non-traumatic chest pain in emergency department, and it is also difficult to classify appropriately. The purpose of this study is to predict probability of acute myocardial infarction(AMI) and assess the group for low probability of AMI that is target for observation-unit of chest pain in emergency department. METHODS: A prospective, clinical study was performed from March 1998 to August 1998 at the department of emergency medicine, Ewha Womans University Mokdong hospital. We classified high-probability group and low-probability group of AMI according to Goldman's algorithm and then compared with the final diagnosis. RESULTS: The study included 218 patients. 84 patients(39%) was the high-probability group and 134 patients(61%) was the low-probability group of AMI. As compared with final diagnosis, AMI was 47 patients(56%) among the high-probability group and 1 patient(0.7%) among the low-probability group. We observed sensitivity of 98%, specificity of 78% in predicting AMI. CONCLUSION: The classification according to Goldman's algorithm may be useful predictor of AMI and improve triage for emergency department patient with chest pain. It is also helpful for management of observation-unit of chest pain in emergency department.
Chest Pain*
;
Classification
;
Diagnosis
;
Emergencies*
;
Emergency Medicine
;
Emergency Service, Hospital*
;
Female
;
Humans
;
Prospective Studies
;
Sensitivity and Specificity
;
Thorax*
;
Triage
9.A Case of unusual condyloma Acuminatum in an Immunosuppressed Patient.
Joo Hyun CHOI ; Mi Kyung CHO ; Duk Kyu CHUN ; Kyoung Chan PARK
Korean Journal of Dermatology 1989;27(6):734-737
Condyloma acuminatum is a common viral disease which is transmitted by sexual intercourse. We experienced a case of condyloma acuminatum occurring in a patient of systemic lupus erythematosus who has been treated with various immunosuppressive agents for 10 years. This condylorna acuminatum was unusual in that it was very rapidly growing, recurrent and reluctant to various treatrnents. We investigated the type of human papillomavirus by Southern blot hybridization and schieved partial improvement by bleomycin intralesional injection together with 5-FU topical application.
Bleomycin
;
Blotting, Southern
;
Coitus
;
Fluorouracil
;
Humans
;
Immunosuppressive Agents
;
Injections, Intralesional
;
Lupus Erythematosus, Systemic
;
Virus Diseases
10.A Case of Delayed Tracheo-esophageal Fistula Complicating Acute Intoxication of Hydrogen Peroxide.
Hyun Young CHO ; Chan Young KOH
Journal of the Korean Society of Emergency Medicine 2017;28(2):218-222
Hydrogen peroxide is a commonly used oxidizing agent. If injected, it may result in morbidity via direct caustic injury, oxygen gas formation, and lipid peroxidation. We report a 40-year-old male patient who accidentally swallowed undiluted hydrogen peroxide (35%). The initial chest computed tomography scan showed no active lesions. He was admitted to the intensive care unit for conservative treatment. Fourteen days after treatment, uncontrolled fever and foul oder sputum occurred, which was not alleviated despite empirical antibiotics therapy. Findings on a chest computed tomography showed tracheo-esophageal fistula at the lower trachea and left main bronchus. He underwent surgical replacement surgery and was discharged without complication after 52 days of admission.
Adult
;
Anti-Bacterial Agents
;
Bronchi
;
Caustics
;
Fever
;
Fistula*
;
Humans
;
Hydrogen Peroxide*
;
Hydrogen*
;
Intensive Care Units
;
Lipid Peroxidation
;
Male
;
Oxygen
;
Poisoning
;
Sputum
;
Thorax
;
Trachea