BACKGROUND: Despite growing awareness of health behavior, making actual behavioral change seems to be more complex. This study assessed factors influencing discrepancy between the awareness and health behavior in Korean middle-aged people. METHODS: A random-digit-dial telephone survey of 1,047 middle-aged Koreans was carried out in 2004. Through the survey, the agreement between the level of awareness and health behavior was analyzed. And factors related to health-risk behaviors in the group of appropriate awareness were analyzed. RESULTS: In the group of appropriate awareness, males tended to be smokers, problem-drinkers and were not maintaining normal weight. In men, younger age, longer work-hours and more frequent stress were significantly related to both smoking and problem-drinking despite appropriate awareness. Higher income and blue-color working class were also predictors for the problem- drinking. In women, older age, lower education, lower income and comorbid disease were significantly related to abnormal weight. Physical inactivity was significantly related to lower education, longer working hours and lower income. CONCLUSION: This study showed that sociodemographic factors and stress were related with health-risk behavior with appropriate awareness in middle-aged people. These findings are expected to have implications for development of health promotion program focusing on stress management, improvement of sociocultural perception and simultaneous intervention for both smoking and alcohol. For middle-aged Korean women, more efforts must be made to remove economical and sociocultural barriers.
Drinking ; Education ; Female ; Health Behavior* ; Health Promotion ; Humans ; Male ; Middle Aged ; Smoke ; Smoking ; Telephone

The EST Knowledge Integrated System, EKIS (http://ekis.kribb.re.kr), was established as a part of Korea's Ministry of Education, Science and Technology initiative for genome sequencing and application research of the biological model organisms (GEAR) project. The goals of the EKIS are to collect EST information from GEAR projects and make an integrated database to provide transcriptomic and metabolomic information for biological scientists. The EKIS constitutes five independent categories and several retrieval systems in each category for incorporating massive EST data from high-throughput sequencing of 65 different species. Through the EKIS database, scientists can freely access information including BLAST functional annotation as well as Genechip and pathway information for KEGG. By integrating complex data into a framework of existing EST knowledge information, the EKIS provides new insights into specialized metabolic pathway information for an applied industrial material.
Data Mining ; Expressed Sequence Tags ; Genome ; Metabolic Networks and Pathways ; Metabolomics ; Models, Biological

JL1, a specific epitope on CD43, is a potential biomarker for the diagnosis of acute leukemia. Although qualitative assays for detecting leukemia-specific CD43 exist, there is a need to develop quantitative assays for the same. Here, we developed two novel monoclonal antibodies (mAbs), 2C8 and 8E10, recognizing different epitopes on CD43. These clones are capable of pairing with YG5, another mAb against JL1 epitope, because they were selectively obtained using sandwich ELISA. Antigens recognized by 2C8 and 8E10 were confirmed as CD43 by western blotting using the CD43-hFC recombinant protein. When expression on various leukemic cell lines was investigated, 2C8 and 8E10 displayed a disparity in the distribution of the epitope. Enzyme assays revealed that these mAbs recognized a sialic acid-dependent epitope on CD43. Using normal thymus and lymph node paraffin-embedded tissues, we confirmed a difference in the epitopes recognized by the two mAbs that was predicted based on the maturity of the cells in the tissue. In summary, we developed and characterized two mAbs, 2C8 and 8E10, which can be used with YG5 in a sandwich ELISA for detecting leukemia-specific CD43.
Antibodies, Monoclonal ; Blotting, Western ; Cell Line ; Clone Cells ; Diagnosis ; Enzyme Assays ; Enzyme-Linked Immunosorbent Assay ; Epitopes* ; Leukemia ; Lymph Nodes ; Thymus Gland