The recent advances in human pluripotent stem cells (hPSCs) enable to precisely edit the desired bases in hPSCs to be used for the establishment of isogenic disease models and autologous ex vivo cell therapy. The knock-in approach based on the homologous directed repair with Cas9 endonuclease, causing DNA double-strand breaks (DSBs), produces not only insertion and deletion (indel) mutations but also deleterious large deletions. On the contrary, due to the lack of Cas9 endonuclease activity, base editors (BEs) such as adenine base editor (ABE) and cytosine base editor (CBE) allow precise base substitution by conjugated deaminase activity, free from DSB formation. Despite the limitation of BEs in transition substitution, precise base editing by BEs with no massive off-targets is suggested to be a prospective alternative in hPSCs for clinical applications. Considering the unique cellular characteristics of hPSCs, a few points should be considered. Herein, we describe an updated and optimized protocol for base editing in hPSCs. We also describe an improved methodology for CBE-based C to T substitutions, which are generally lower than A to G substitutions in hPSCs.

PURPOSE: Metabolic syndrome (MS) is a clinical condition that shares many common characteristics with diabetes. However, unlike diabetes, the usefulness of MS as a prognostic entity in peripheral arterial disease is uncertain. This study evaluated the prognostic usefulness of MS in critical lower limb ischemia (CLI) patients. MATERIALS AND METHODS: We compared the 2-year clinical outcomes in 101 consecutive CLI patients (66+/-14 years; 78% men) with 118 affected limbs treated with percutaneous transluminal angioplasty (PTA) according to the presence of MS and diabetes. RESULTS: The number of MS patients was 53 (52%), of which 45 (85%) had diabetes. During a 2-year follow-up, the incidence of clinical outcomes, including reintervention, major amputation, minor amputation, and survival, was not significantly different between MS and non-MS patients; however, the incidence of minor amputation was significantly higher in diabetic than in non-diabetic patients (42% vs. 17%; p=0.011). Cox regression analysis for the 2-year primary patency demonstrated no association between MS and 2-year primary patency [hazard ratio (HR), 1.02; 95% confidence interval (CI), 0.45-2.30; p=0.961], whereas there was a significant association between diabetes and 2-year primary patency (HR, 2.81; 95% CI, 1.02-7.72; p=0.046). Kaplan-Meier analysis revealed no significant difference in the 2-year primary patency between MS and non-MS patients; however, the 2-year primary patency was lower in diabetic than in non-diabetic patients (p=0.038). CONCLUSION: As a prognostic concept, MS might conceal the adverse impact of diabetes on the prognosis of CLI patients treated with PTA.
Aged ; Aged, 80 and over ; Angioplasty/*methods ; Diabetes Mellitus/*therapy ; Female ; Humans ; Ischemia/*therapy ; Lower Extremity/*blood supply ; Male ; Metabolic Syndrome X/*therapy ; Middle Aged ; Republic of Korea ; Retrospective Studies

BACKGROUND: An increase in the maximum level of sensory blockade by epidural 'top-up' in combined spinal epidural anesthesia may be explained by two mechanisms; a volume effect, compressing the dural sac, and a local anesthetic effect. This study was undertaken to investigate the relative importance of these factors. METHODS: Eighty patients about to undergo lower limb surgery under combined spinal epidural anesthesia were randomly assigned to four groups. Using the needle-through-needle technique, all patients received a subarachnoid injection of 8 mg of heavy bupivacaine through an epidural catheter. At 30 minutes after subarachnoid injection, an epidural top-up was given according to a randomization code. Experimental groups received 10 ml of saline, 10 ml of bupivacaine 0.25%, or 10 ml of bupivacaine 0.5%. The control group received no epidural top-up. The level of sensory blockade was checked by using the pinprick test at 5, 10, 15, 20, 25 and 30 minutes after subarachnoid injection, and at 5, 10, 15, 20, 25 and 30 minutes after epidural top-up. Blood pressure, heart rate and the incidence of side effects such as hypotension, bradycardia, nausea, and high block were analyzed. RESULTS: There was no significant difference in maximum level of sensory block among the 10 ml saline, 10 ml bupivacaine 0.25% or 10 ml bupivacaine 0.5% treated groups during epidural phase. Although blood pressure and heart rate were decreased, there were no differences among experimental groups during epidural phase. The most common side effect during combined spinal epidural anesthesia was hypotension. The incidence of bradycardia and high sensory block (above T4 dermatome level) was similar among the groups. CONCLUSIONS: After the maximum level of sensory blockade due to subarachnoid injection has been established, an epidural top-up with 10 ml of saline or 10 ml of either 0.25% or 0.5% bupivacaine did not significantly increase the level of subarachnoid block in patients with lower extremity surgery. The author concludes the there were no differences among groups with regard to the volume effect and local anesthetic effect in terms of the sensory blockade level during the epidural phase. However, blood pressure and heart rate in the epidural top-up groups reduced with operation time.
Anesthesia ; Anesthesia, Epidural* ; Anesthetics ; Blood Pressure ; Bradycardia ; Bupivacaine* ; Catheters ; Heart Rate ; Humans ; Hypotension ; Incidence ; Lower Extremity ; Nausea ; Random Allocation

BACKGROUND AND OBJECTIVES: The eosinophil plays an important role in allergic inflammation and the eosinophil cationic proteins (ECP) is one of the major granular proteins during its activation. ECP is proved to have an eosinophil specificity and reflects the activity of the eosinophil in the target site. We tried to identify the difference in the ECP levels between the nasal secretion of allergic and nonallergic rhinitis. MATERIALS AND METHODS: In order to draw a correlation between the ECP level and other diagnostic methods of allergy, we examined ECP in serum and nasal secretion of 20 patients with mite allergy and 20 non-allergic rhinitis patients. We also performed symptom questionnaires, acoustic rhinometry, the Wright-Giemsa staining of nasal brushings, and total amount of IgE in nasal secretion. RESULTS: ECP level in the nasal secretion of allergic patients after allergen challenge was higher than that of non-allergic rhinitis patients (p=0.026). We also found that the ECP level of allergic patients was higher after allergen provocation than before (p=0.005). Additionally, there was a negative correlation between the minimal cross-sectional area in acoustic rhinometry and the ECP level in the nasal secretion before the challenge (r=- 0.54, p=0.01). CONCLUSION: We concluded that ECP is the soluble marker which reflects the severity of allergic inflammation in the nose and is useful for diagnosis as well as in the follow-up care of nasal allergic inflammation.
Diagnosis ; Eosinophil Cationic Protein* ; Eosinophils* ; Humans ; Hypersensitivity ; Immunoglobulin E ; Inflammation ; Mites ; Nose ; Surveys and Questionnaires ; Rhinitis* ; Rhinometry, Acoustic ; Sensitivity and Specificity

Synchronous primary cancers in the liver and gallbladder have been rarely reported.We report a case of synchronous cancers of hepatic angiosarcoma and gallbladder adenocarcinoma, mimicking gallbladder cancer with hepatic invasion. Additionally, the clinical implications, the radiologic features, and the diagnostic difficulties are further discussed.

Liposarcoma in esophagus is rare, moreover the pedunculated form is very rare. We experienced a case of liposarcoma in a 36-year-old man who had intermittent swallowing difficulty for 7 months. Endoscopy and esophagography revealed that a smooth longitudinal tumor mass occupied the esophageal lumen. Esophagotomy and surgical excision was done. The tumor was 4 cm in length and 3 cm in average diameter with an obvious stalk measuring 3.5 cm in length and 1.5 cm in diameter. Microscopic examination disclosed a lipoma with focal ulceration and liposarcomatous change infiltrating into interstitial fibrous tissue at its distal end.
Adult ; Deglutition ; Endoscopy ; Esophagus* ; Humans ; Intestines* ; Lipoma ; Liposarcoma* ; Stomach* ; Ulcer

STUDY DESIGN: We retrospectively assessed the results of percutaneous balloon kyphoplasty (KP) by clinical and radiological methods. PURPOSE: To evaluate the outcome of KP as a treatment for osteoporotic burst fractures. OVERVIEW OF LITERATURE: Many surgeons are concerned about the possibility of neurological complications after percutaneous kyphoplasty for osteoporotic burst fractures, secondary to intra-canal cement leakage. METHODS: We performed KP as a treatment for osteoporotic burst fractures. We studied 12 patients/13 vertebrae. The two control groups consisted of patients who only underwent conservative treatment and those who underwent posterior instrumentation and fusion. We measured each preoperative/postoperative vertebral kyphotic deformity angle (KDA) using simple lateral spine images and checked for leakage of cement, as well. The preoperative/postoperative visual analog scale (VAS) scores for back pain, degree of daily activity, and postoperative complications were evaluated. RESULTS: The mean improvement in KDA after KP was 9.7+/-2.2degrees. The mean preoperative and postoperative VAS scores for back pain were 8.3+/-0.4 and 3.1+/-0.17, respectively. Regarding the control group, the mean postoperative VAS score for the conservative group and the posterior surgery group decreased by 4.5+/-0.17 and 3.2+/-0.19, respectively. There was no statistically significant difference between the KP and posterior surgery groups (p=0.125). However, there was a statistically significant difference between the KP and conservative treatment groups (p=0.012). CONCLUSIONS: KP is safe and useful for treating osteoporotic burst fractures.
Back Pain ; Congenital Abnormalities ; Humans ; Kyphoplasty ; Postoperative Complications ; Retrospective Studies ; Spine

Symphysis pubis separation is an uncommon but not rare complication of delivery. Characteristic symptoms of symphyseal separation include suprapubic pain and tenderness which radiate to the back or legs, difficult ambulation, and bladder dysfunction. Clinical history, presenting symptoms, and response to therapy are sufficient to make the diagnosis, although radiographic documentation of symphyseal separation by x-ray or ultrasound are frequently used to confirm the diagnosis. The underlying etiology of symptomatic symphyseal separation has not been fully elucidated. Associations with macrosomia, pathological joint loosening, and increased force placed on the pelvic ring have been suggested as possible etiologies. Conservative therapy, including bed rest, pelvic binders, ambulation devices, and mild analgesics usually result in complete recovery within 4-16 weeks. Our experience of three cases of peripartum symphysis pubis separation delivered from 1998 to 1999 were reviewed with related articles.
Analgesics ; Bed Rest ; Diagnosis ; Joints ; Leg ; Peripartum Period ; Pregnancy ; Ultrasonography ; Urinary Bladder ; Walking

Although several publications have reported delayed or non-union, there is a consensus that the standard treatment for atypical femoral fracture (AFF) is an intramedullary nailing. However, no case of tensile insufficiency fracture of femoral neck associated with intramedullary nailing in patients with AFF have been reported. Here, we report an 82-year-old woman with tensile type of insufficiency fracture of the femoral neck after intramedullary nailing for the treatment of AFF.
Aged, 80 and over ; Consensus ; Female ; Femoral Fractures* ; Femur Neck* ; Fracture Fixation, Intramedullary* ; Fractures, Stress* ; Humans ; Osteoporosis

BACKGROUND: Anatomic footprint restoration of anterior cruciate ligament (ACL) is recommended during reconstruction surgery. The purpose of this study was to compare and analyze the femoral and tibial tunnel positions of transtibial single bundle (SB) and transportal double bundle (DB) ACL reconstruction using three-dimensional computed tomography (3D-CT). METHODS: In this study, 26 patients who underwent transtibial SB ACL reconstruction and 27 patients with transportal DB ACL reconstruction using hamstring autograft. 3D-CTs were taken within 1 week after the operation. The obtained digital images were then imported into the commercial package Geomagic Studio v10.0. The femoral tunnel positions were evaluated using the quadrant method. The mean, standard deviation, standard error, minimum, maximum, and 95% confidence interval values were determined for each measurement. RESULTS: The femoral tunnel for the SB technique was located 35.07% +/- 5.33% in depth and 16.62% +/- 4.99% in height. The anteromedial (AM) and posterolateral (PL) tunnel of DB technique was located 30.48% +/- 5.02% in depth, 17.12% +/- 5.84% in height and 34.76% +/- 5.87% in depth, 45.55% +/- 6.88% in height, respectively. The tibial tunnel with the SB technique was located 45.43% +/- 4.81% from the anterior margin and 47.62% +/- 2.51% from the medial tibial articular margin. The AM and PL tunnel of the DB technique was located 33.76% +/- 7.83% from the anterior margin, 45.56% +/- 2.71% from the medial tibial articular margin and 53.19% +/- 3.74% from the anterior margin, 46.00% +/- 2.48% from the medial tibial articular margin, respectively. The tibial tunnel position with the transtibial SB technique was located between the AM and PL tunnel positions formed with the transportal DB technique. CONCLUSIONS: Using the 3D-CT measuring method, the location of the tibia tunnel was between the AM and PL footprints, but the center of the femoral tunnel was at more shallow position from the AM bundle footprint when ACL reconstruction was performed by the transtibial SB technique.
Adult ; Anterior Cruciate Ligament Reconstruction/*methods ; *Femur/radiography/surgery ; Humans ; Imaging, Three-Dimensional/*methods ; Knee Joint/physiology ; Male ; Prospective Studies ; Surgery, Computer-Assisted/*methods ; *Tibia/radiography/surgery ; Tomography, X-Ray Computed