1.Production of IL - 6 and IL - 8 in Human Fibroblasts Stimulated with Mycoplasma Lysates and Bacterial Toxins.
Kwang Hyuk KIM ; Myung Woong CHANG
Journal of the Korean Society for Microbiology 1999;34(6):573-582
Cytokines are hormone-like proteins which mediate and regulate inflammatory and immune responses. The purpose of this study was to investigate the effect of lipopolysaccharide (LPS), Staphylococcus enterotoxin B (SEB), and Mycoplasma lysates on regulation of IL-6 and IL-8 production by human nasal fibroblasts. Primary cultured cells were incubated with LPS (1.0 ug/ml) from E.coli, SEB (1.0 ug/ml) from S.aureus, or Mycoplasma lysates (M.pneumoniae, Mp; M. fermentans, Mf; M. hominis, Mh, each 1.0 ug/ml). The culture supernatants were collected at 2, 6, and 24 hr and assessed for IL-6 and IL-8 production by enzyme-linked immunosorbent assay. The production of IL-6 in the culture supematant was downregulated by LPS, SEB, or Mycoplasma lysates. But IL-6 was upregulated by mixed exposure with Mp+LPS (2 hr), Mp+LPS+SEB (24 hr), Mf+LPS (24 hr), Mf+LPS+SEB (2 hr), Mh+LPS (24 hr), Mh+SEB (24 hr), or Mh+LPS+ SEB (24 hr). The production of IL-8 in the culture supematant was similar to that of IL-6 by same stimulants. But IL-8 was upregulated by mixed exposure with Mf+LPS+SEB (2 hr), Mh+LPS (24 hr), Mh+ SEB (24 hr), or Mh+LPS+SEB (24 hr). These studies show that costimulation of LPS or SEB with Mycoplasma whole cell lysates upregulates the production of IL-6 and IL-8.
Bacterial Toxins*
;
Cells, Cultured
;
Cytokines
;
Enterotoxins
;
Enzyme-Linked Immunosorbent Assay
;
Fibroblasts*
;
Humans*
;
Interleukin-6
;
Interleukin-8
;
Mycoplasma*
;
Staphylococcus
2.Clinical Study of Therapeutic Effect in Status Epilepticus.
Young Hyuk LEE ; Chang Jun COE
Journal of the Korean Pediatric Society 1986;29(1):72-78
No abstract available.
Status Epilepticus*
4.Detection of contaminated mycoplasmas on the cultured cell lines.
Myung Woong CHANG ; Kwang Hyuk KIM
Journal of the Korean Society for Microbiology 1993;28(3):209-221
No abstract available.
Cells, Cultured*
;
Mycoplasma*
5.Isolated Musculocutaneous Nerve Palsy after the Reverse Total Shoulder Arthroplasty.
Sung Guk KIM ; Chang Hyuk CHOI
Clinics in Shoulder and Elbow 2016;19(2):101-104
Reverse total shoulder arthroplasty has been performed with promising results in rotator cuff tear arthropathy. However, the global complication of the reverse total shoulder arthroplasty is relatively higher than that of the conventional total shoulder arthroplasty. Neurologic complications after reverse total shoulder arthroplasty are rare but there are sometimes remaining sequelae. The cause of the neurologic complication is multifactorial, including arm traction, position and the design of the implant. Most cases of neurologic palsy following reverse total shoulder arthroplasty occur in the axillary nerve and the radial nerve. The authors report on a case of a 71-year-old man with isolated musculocutaneous nerve palsy after reveres total shoulder arthroplasty with related literature.
Aged
;
Arm
;
Arthroplasty*
;
Humans
;
Musculocutaneous Nerve*
;
Paralysis*
;
Radial Nerve
;
Rotator Cuff
;
Shoulder*
;
Tears
;
Traction
6.Prevention of Heterotopic Bone Formation after Total Hip Arthroplasty in High Risk Patient with Radiation Therapy
Chang Dong HAN ; Chong Hyuk CHOI ; Chang Ok SUH
The Journal of the Korean Orthopaedic Association 1994;29(7):1738-1744
Heterotopic ossification(HO) is a frequent complication of total hip arthroplasty(THA), If it is sevre enough, it can convert a successful result of the operation into a poor one. Among several strategies that researches in recent years have tested, radiation therapy have been accepted to have efficacy for prevention of heterotopic ossification by many authors. While postoperative irradiation has been shown to lower the incidence of HO effectively in high risk patients following THA, patients in multiple dose protocols continue to develop some heterotopic ossifiction despite therapy and have some problems. Ninteen patients received single dose exposure to 600 rad delivered within 48 hours of THA with shielding of prosthesis region. The patients were considered high risk group for developing HO because of hypertropic osteoarthritis, post-traumatic osteoarthritis or the presence of previous formed ectopic bone. The average follow up period was 10.2 months(range 6 months
Arthroplasty, Replacement, Hip
;
Follow-Up Studies
;
Hip
;
Humans
;
Incidence
;
Ossification, Heterotopic
;
Osteoarthritis
;
Osteogenesis
;
Prostheses and Implants
7.Prevalence of Ureaplasma Urealyticum in Ovarian or Cervical Cancer.
Myung Woong CHANG ; Kwang Hyuk KIM ; In Dal PARK
Journal of the Korean Society for Microbiology 2000;35(5):361-361
No Abstract Available.
Prevalence*
;
Ureaplasma urealyticum*
;
Ureaplasma*
;
Uterine Cervical Neoplasms*
8.The clinical characteristics of insomnia in general hospital inpatients.
Jong Gook LEE ; Chang Hwan HAN ; Tae Hyuk YOO
Journal of Korean Neuropsychiatric Association 1991;30(1):125-134
No abstract available.
Hospitals, General*
;
Humans
;
Inpatients*
;
Sleep Initiation and Maintenance Disorders*
9.Clinical Evaluation of the Arachnoid Cysts in the Pediatric Age Group.
Ho Taek KIM ; Young Hyuk LEE ; Chang Jun COE
Journal of the Korean Pediatric Society 1988;31(4):467-473
No abstract available.
Arachnoid Cysts*
;
Arachnoid*
;
Humans
10.A study of artifacts in MR imaging induced by metalic aneurysm clips.
Joo Hyuk LEE ; Kee Hyun CHANG ; Jae Hyung PARK
Journal of the Korean Radiological Society 1992;28(2):307-313
To investigate MR artifacts induced by metallic implants, scans were obtained using both the ferromagnetic Drake lip and the non ferromagnetic Yasargil clip. Scan were taken through the area of clips using geometrical phantom. The MRI was performed by spin echo technique and gradient echo technique on both 2.0T and 0.5T MR unit. The luthors evaluated the nature and differences of artifacts in each sequence and parameter. Artifacts induced by both lips were noted in the direction of frequency encoding gradient, and consisted of region of signal loss abutted in one side by survilinear region of bright signal. Geometric distortion of image was marked in the Drake clip, out was minimal in the Yasargil clip under both 2.0T and 0.5T. Artifacts were more pronounced in the gradient echo technique than those of the spin echo technique on both 2.0T and 0.5T. Although there were no differences n the nature of artifacts induced by the Drake clip among each parameter in the spin echo technique under 2.0T, artifacts were slightly more pronounced on T2 weighted image under the 0.5T field, but no differences were found in the nature of artifacts induced by the Yasargil clip in the spin echo technique under, both the 2.0T and 0.5T fields. Marked artifacts were induced through the small area of the Drake clip which were included in the scan plane, but induced artifacts were small when a small area of the Yasargil clip was included in the scan plane. It seemed likely hat artifacts were slightly more pronounced in the 2.0T than the 0.5T field in both clips, but objective evaluation of the difference was difficult, In conclusion, these results can be an essential basis for the interpretation of MR images or patents with metallic inplants.
Aneurysm*
;
Artifacts*
;
Lip
;
Magnetic Resonance Imaging*
;
Magnets