1.Effect of hypocapnia on extracellular glutamate and glycine concentrations during peri-ischemic period in the rabbit hippocampus.
Kyu Taek CHOI ; Jung Kil CHUNG ; Chun Sik KWAK ; Hae Kyu KIM
Journal of Korean Medical Science 1994;9(5):394-401
Glutamate (GLU) is a neurotransmitter. Massive release of GLU and glycine (GLY) into the brain's extracellular space may be triggered by ischemia, and may result in acute neuronal lysis or delayed neuronal death. The aim of this study was to evaluate the possible relationship between hyperventilation and the level of GLU and GLY during brain ischemia. Rabbits were anesthetized with halothane and oxygen. Group 1 was allowed to hyperventilate (PaCO2 25-35 mmHg). PaCO2 was maintained throughout the study. Group 2 was a normal control group that maintained normocapnia. Two global cerebral ischemic episodes were produced. Microdialysate was collected during the peri-ischemic and reperfusion periods from the dorsal hippocampus. GLU and GLY concentrations were determined using high-performance liquid chromatography. In the control group, GLU and GLY were significantly elevated during each episode of ischemia; these levels returned to baseline within 10 minutes after reperfusion. In contrast, in the hyperventilation group GLU and GLY concentrations increased during ischemia, but they were not statistically significant. We were able to demonstrate that hypocapnia during periischemic period lowered extracellular GLU and GLY concentrations. These results can explain a part of the protective action of hypocapnia during cerebral ischemia.
Animal
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Brain Ischemia/*metabolism
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Glutamic Acid/*analysis
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Glycine/*analysis
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Hippocampus/*chemistry
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Hyperventilation/metabolism
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Hypocapnia/*metabolism
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Potassium/metabolism
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Potassium Channels/physiology
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Rabbits
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Support, Non-U.S. Gov't
2.Hypocapnia Attenuates, and Nitrous Oxide Disturbs the Cerebral Oximetric Response to the Rapid Introduction of Desflurane.
Younsuk LEE ; Jeoung Hyuk LEE ; Dong Il YOON ; Youngmin LEE ; Kyoung Ok KIM ; Seunghyun CHUNG ; Junyong IN ; Jun Gwon CHOI ; Hun CHO
Journal of Korean Medical Science 2009;24(6):1051-1057
The aim of this study was to develop a nonlinear mixed-effects model for the increase in cerebral oximetry (rSO2) during the rapid introduction of desflurane, and to determine the effect of hypocapnia and N2O on the model. Twelve American Society of Anesthesiologist physical status class 1 and 2 subjects were allocated randomly into an Air and N2O group. After inducing anesthesia, desflurane was then increased abruptly from 4.0 to 12.0%. The PET(CO2), PET(DESF) and rSO2 were recorded at 12 predetermined periods for the following 10 min. The maximum increase in rSO2 reached +24-25% during normocapnia. The increase in rSO2 could be fitted to a four parameter logistic equation as a function of the logarithm of PET(DESF). Hypocapnia reduced the maximum response of rSO2, shifted the EC50 to the right, and increased the slope in the Air group. N2O shifted the EC50 to the right, and reduced the slope leaving the maximum rSO2 unchanged. The N2O-effects disappeared during hypocapnia. The cerebrovascular reactivity of rSO2 to CO2 is still preserved during the rapid introduction of desflurane. N2O slows the response of rSO2. Hypocapnia overwhelms all the effects of N2O.
Adult
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Anesthetics, Inhalation/*pharmacology
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*Cerebral Cortex/blood supply/drug effects/physiology
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Cerebrovascular Circulation/*drug effects/physiology
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Female
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Hemodynamics
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Humans
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Hypocapnia/*metabolism
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Isoflurane/*analogs & derivatives/pharmacology
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Male
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Middle Aged
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Models, Theoretical
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Nitrous Oxide/*metabolism
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*Oximetry
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Random Allocation
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Regional Blood Flow/drug effects