2.Salivary Bacterial Counts on Application Time of Oral Antiseptic Agents and Mechanical Irrigation
Hyoung Sup LIM ; Jae Jin KIM ; Mija KIM ; Hak Kyun KIM
Journal of the Korean Association of Maxillofacial Plastic and Reconstructive Surgeons 2013;35(3):155-160
Agar
;
Bacteria
;
Bacterial Load
;
Chlorhexidine
;
Mouth
;
Povidone-Iodine
;
Saliva
;
Sheep
;
Stem Cells
3.A Novel Cell Line for Screening of Immunosuppressor Specific to T Lymphocytes.
Sang Kyou LEE ; Jung Hee LIM ; Kyung Min CHO ; Seung Hyo LEE ; Yong Sup SONG ; Hyoung Sik CHUN ; Hyeog Jin SON
Korean Journal of Immunology 1997;19(3):375-382
The systematic study of products from bacteria and fungi has led to the development of two immunosuppressive drugs, cyclosporin A and FK 506 (tacrolimus) which are useful to suppress adaptive immune responses to the grafted tissue. However, they affect all immune responses indiscriminately and are both toxic to kidneys and other organs. To facilitate the development of immunosuppressor to block the T cell receptor (TcR)-mediated signaling cascade specifically, a novel Jurkat T cell transfectants, JK NFAT-SEAP were generated in which the expression of the secreted alkaline phosphatase (SEAP) is driven by the multiple NFAT binding sites plus minimal IL-2 promoter. Upon stimulation with ionomycin or anti-TcR mAb OKT3 in the presence of PMA, these transfectants secreted high level of SEAP into the medium, which was conveniently analyzed by SEAP analysis. The secretion of SEAP was effectively inhibited by cyclosporin A or FK 506 at the concentration of [10 ' ug/ml], [10 ug/ml] respectively. JK NFAT-SEAP transfectants will provide two major advantages for the development of a novel immunosuppressor. First, analysis of SEAP secreted into the culture medium by SEAP analysis enables us to test a large number of samples within a short period of time. Second, Usage of IL-2 promoter for the expression of SEAP makes us identify bioproducts to target specifically on TcR-mediated signaling pathway.
Alkaline Phosphatase
;
Bacteria
;
Binding Sites
;
Cell Line*
;
Cyclosporine
;
Fungi
;
Interleukin-2
;
Ionomycin
;
Kidney
;
Mass Screening*
;
Muromonab-CD3
;
Receptors, Antigen, T-Cell
;
T-Lymphocytes*
;
Tacrolimus
;
Transplants
4.Protective Effect of Capsaicin on the Ischemia-Reperfusion Injury of the Intestine in Rats.
Won Young KIM ; Kyoung Soo LIM ; Hyoung Sup PARK
Journal of the Korean Society of Emergency Medicine 2002;13(2):155-162
PURPOSE: Capsaicin, a major ingredient of hot peppers, has a stimulatory effect on the neurons. Ingested capscaicin causes pain and even damages the mucosa with a large dose. On the other hand, it protects the gastric mucosa against many ulcerogenic agents or conditions. In this study, we investigated the effect of oral capsaicin on the ischemia-reperfusion damage of the intestinal mucosa in rats. The involvement of stress proteins such as HSP-70 and HSP-72 in the action of capsaicin was also investigated. METHODS: Male Sprague-Dawley rats were given oral capsaicin (20 mg/kg/day) for a designated period. Ischemia of the small intestine was inflicted by clamping the superior mesenteric arteries for 30 minutes under anesthesia. After the ischemic period, the clamp was released and the animal was kept for 1, 5, or 24 hours. Then, the intestinal structure was observed under the microscope, and the levels of the heat-shock proteins were determined by using a Western blot. RESULTS AND CONCLUSION: Oral capsaicin protected the mucosa of the small intestine against the ischemia/reperfusion injury. Capsaicin also increased and expedited the ischemia/reperfusion-induced expression of the heat-shock proteins, HSP-70 and 72, in the intestine. The possible linkage between the two events is discussed.
Anesthesia
;
Animals
;
Blotting, Western
;
Capsaicin*
;
Constriction
;
Gastric Mucosa
;
Hand
;
Heat-Shock Proteins
;
Humans
;
Intestinal Mucosa
;
Intestine, Small
;
Intestines*
;
Ischemia
;
Male
;
Mesenteric Artery, Superior
;
Mucous Membrane
;
Neurons
;
Rats*
;
Rats, Sprague-Dawley
;
Reperfusion Injury*
5.A case of SIADH in small cell lung cancer.
Kyu Chang WON ; Jong Sik LIM ; Chan Woo LEE ; Hyoung Woo LEE ; Choong Ki LEE ; Jin Hong CHUNG ; Myoung Soo HYUN ; Bong Sup SHIM ; Hyun Woo LEE
Yeungnam University Journal of Medicine 1991;8(2):227-234
The syndrome of inappropriate ADH secretion is a disorder characterized by hyponatremia which results from water retention attributable to ADH release. The hallmark of SIADH is hyponatremia due to water retention, in the presence of urinary osmolality above plasma osmolality. The SIADH was initially described by Schwartz et al (1957). This syndrome, first recognized in patients with bronchogenic carcinoma, has now been observed in a variety of other illnesses. Recently, we encountered a 59 year-old female with small cell lung cancer, also she had SIADH. Thus, we present a case and review the literature on the subject.
Carcinoma, Bronchogenic
;
Female
;
Humans
;
Hyponatremia
;
Inappropriate ADH Syndrome*
;
Osmolar Concentration
;
Plasma
;
Small Cell Lung Carcinoma*
;
Water
8.Trans-Sacral Local Resection as a Posterior Approach.
Bong Hwa LEE ; Hyoung Chul PARK ; Hae Wan LEE ; Chang Nam AN ; Taeik UM ; Young A LIM ; Byoung Sup KIM ; Mi Young CHANG ; Soo Hyoung KIM ; Sung Wook CHO
Journal of the Korean Society of Coloproctology 2010;26(3):197-203
PURPOSE: Surgical removal for a mass in the pre-sacral space or mid rectum through a posterior approach is not frequent. We would like to present the technique of trans-sacral local resection as a posterior approach. We analyzed the follow up of patients who underwent surgery using the proposed technique. METHODS: A total of 21 patients who had undergone a trans-sacral local resection with lower sacrectomy between January 1997 and December 2006 were enrolled in this study. The diagnoses were large epidermal cyst, gastrointestinal stromal tumor, high grade adenoma, and early cancers in the mid rectum. We analyzed the surgical complications and disease recurrences. The mean follow up for tumors of the rectum was 53+/-35 mo. RESULTS: Epidural anesthesia was appropriate for all whole procedures. Among the 21 cases, there was one case of a rectocutaneous fistula as a postoperative complication (4.9%). In one case among the submucosal cancers, there was a systemic metastasis at 24 mo without local recurrence. CONCLUSION: In our experience, a trans-sacral resection with a lower sacrectomy is a good option and provides a wide and direct surgical exposure for the removal of a pre-sacral or a mid-rectal mass. Good bowel preparation is mandatory.
Adenoma
;
Anesthesia, Epidural
;
Epidermal Cyst
;
Fistula
;
Follow-Up Studies
;
Gastrointestinal Stromal Tumors
;
Humans
;
Neoplasm Metastasis
;
Postoperative Complications
;
Rectal Neoplasms
;
Rectum
;
Recurrence
9.Experimental Study of Osseointegration and Stability of Intentionally Exposed Hydroxyapatite Coating Implants
Kyung In JEONG ; Su Gwan KIM ; Seong Yong MOON ; Ji Su OH ; Ji Ho JO ; Hyoung Sup LIM ; Jeong Sun KIM ; Sung Chul LIM ; Mi Ae JEONG
Journal of the Korean Association of Maxillofacial Plastic and Reconstructive Surgeons 2012;34(1):12-16
Animals
;
Dental Implants
;
Dogs
;
Durapatite
;
Femur
;
Humans
;
Intention
;
Mentors
;
Osseointegration
;
Osteogenesis
;
Reference Values
10.P MR spectroscopy study of the brain at 4.7 T in new borns.
Dae Chul SUH ; Chi Woong MOON ; Tae Keun LEE ; Ki Soo KIM ; Yun YI ; tae Hwan LIM ; Cheol Min PARK ; Hye Young CHOI ; Hyoung Sup PARK ; On You HWANG ; Shi Joon YOO
Journal of the Korean Radiological Society 1993;29(1):152-159
In vivo 31P NMR spectra were obtained in eight infant brain at 4.7T. Each phosphorus metabolite and its ratio were analyzed to evaluate the brain damage and maturity, and compared with the reported data obtained at the lower field strength. Measurement of T1 relaxation time at 4.7T was done in an infant and a cat brain in vivo. PCr/Pi and PCr/β-ATP ratio were used as a marker of brain damage. PME/PDE revealed higher values than those of the reported data obtained at the lower field strength and the difference was partly attributed to the long T1 relaxation time of PME rather than the brain immaturity. Although the resolution of the spectrum was improved at 4.7T, a long repetition time is recommended to minimize T1difference of phosphorus metabolites of brain at 4.7T.
Animals
;
Brain*
;
Cats
;
Humans
;
Infant
;
Magnetic Resonance Spectroscopy*
;
Phosphorus
;
Relaxation