BACKGROUND: The influencion the environment on a culture is expressed via four routes. (1) the nature of the substrate or phase on or in which the cells grow (2) the physicochemical and physiological constitution of the medium, (3) the constitution of the gas phase, and (4) the incubation temperature. Melanization is closely related to the constitution and amounts of amino acids in the medium. OBJECTIVE: The aim of this study is to investigate whether there are some differences of proliferation and melanization in cultured B,F, mouse melanoma cells according to different culture media. METHODS: We examined the color of cell pellet, cell morphology, electron microscopic findings, cell counts and melanin conlensin BgF mouse melanoma cells cultured in Dulbeccos modified Eagles medium(DMEM), F-10, MCDB 153, Minimal essential medium(MEM), and RPMI 1640, respectively. RESULTS: 1. The color of cell pellet., ringed from dark gray to light brown. The order of the darkness was DMEM, MEM, RPMI 1640, MCDB 153, and F-10 medium. 2. Most Bg, mouse melanoma cells had an epithelioid morphology, but a few cells in MCDB 153 medium showed dendrites. On the 4th day after culture, the cells in F-10 medium were larger than those in the other media. 3. In the electron microscopic. findings, BF, mouse melanoma cells in DMEM and MEM con tained numerous stage IV nelanosomes, however, those in RPMI 1640 and MCDB 153 medium contained a few, and those in F-10 medium did few. 4. The number of BF, mouse melanoma cells were 1.42 + 0.06 x 10", 1.42 + 0.12 x 10", l. 17 + 0.08 x 10, 0.73 0.06 x 10, 0.32 0.01 x 10, in RPMI 1640, DMEM, MEM, F 10, and MCDB 153 medium, respectively. 5. In the MTT assay, the order of the optical density of B,F, mouse melanoma cells in various media was as followings, DMEM, RPMI 1640, MEM, F-10, and MCDB 153. 6. Compared with the melanin contents of B;F, mouse melanoma cells in DMEM, they were 77.97% in MEM, 67.91% in RPMI 1640 and MCDB 153 medium, and 55.94% in F-10 medium. CONCLUSION: The phenotypic changes of BF, mouse melanoma cells were induced by various culture rnedia and were reversilvle. Since the phenotypes of cells can be changed by the culture media, researchers should choose the appropriate culture medium for the cells.
Amino Acids ; Animals ; Cell Count ; Constitution and Bylaws ; Culture Media ; Darkness ; Dendrites ; Eagles ; Melanins ; Melanoma* ; Mice* ; Phenotype

In 1968, Hardy and Anderson first described the term hypereosinophilic syndrome. This syndrome is characterized by persistent and prolonged eosinothila, with primarily hematologic, cardiac, neurologic and derrnatologic abnormalities. Cutaneous mmestations occur in 27-57% of patients. Two types of skin lesions have been noted: (1) erythrritous pruritic papules and nodules or (2) urticaria and angioedema. We report a case of hypereosinophilic syndrome with a vesicle eruption which is a rare skin lesion in tbis syndrome, in a 17 year old man. Diagnosis of hyprcsinophilic syndrome was established by clinical findings, rearked blood eosinophilia without a Brown cause, bone marrow aspiration and biopsy, liver scan and ultrasonography, and histopatholcgie findings of the skin.
Adolescent ; Angioedema ; Biopsy ; Bone Marrow ; Diagnosis ; Eosinophilia ; Humans ; Hypereosinophilic Syndrome* ; Liver ; Skin ; Ultrasonography ; Urticaria

No abstract available.
Esophageal Neoplasms*

Boric acid(H BO) is a weak topical anti infective agent. It can cause acute borism due to the internal absorption. Although the frequency is very rare, boric acid can also induce contact dermat.itis after topical spplication. We present allergic contact dermatitis to boric acid. The patch test results showed a positive reaction to 2% boric acid solution.
Absorption ; Dermatitis, Allergic Contact* ; Patch Tests

No abstract available.
Pneumothorax*

No abstract available.

No abstract available.
Heart Valves* ; Heart*

BACKGROUND:Acute vestibular neuronitis is the disease of which the etiology and pathophysiology are largely unknown . But the viral infection and ischemia of the labyrinth and the vestibular nerve are considered as general etiology. This study was performed to support the viral infection rather than the ischemic theory. MATERIALS & METHODS:We studied seventy years old female patient who showed painful vesicles on left auricle and vertigo with spontaneous nystagmus to the right side. We performed physical examination, serologic test, ENG test, pure tone audiogram, brain magnetic resonance imaging and polymerase chain reaction. RESULTS:We found small vesicles and vascular injection in left EAC, herpes zoster IgG positive, spontaneous right beating in electronystagmograpy, 54% left canal paresis in Caloric test , decreasing left side Tc in velocity step rotatory test, decresed gain, deviation to left in symmetry and phase lead in sinusoidal harmonic acceleration test, normal range hearing in pure tone audiogram, microangiopathy on cortex in brain MRI and negative PCR. CONCLUSION:This case supports viral infection etiology rather than ischemia in vestibular neuritis. But more studies to find the etiology of vestibular neuronitis are required.
Acceleration ; Brain ; Caloric Tests ; Ear, Inner ; Female ; Hearing ; Herpes Zoster ; Herpes Zoster Oticus ; Humans ; Immunoglobulin G ; Ischemia ; Magnetic Resonance Imaging ; Paresis ; Physical Examination ; Polymerase Chain Reaction ; Reference Values ; Serologic Tests ; Vertigo ; Vestibular Nerve ; Vestibular Neuronitis

PURPOSE: This study was done to identify self-directed learning readiness, achievement goal orientations, learning satisfaction and learning achievement, and to evaluate the factors affecting learning achievement for nursing students using a web-based Health Assessment e-Book. METHODS: The research design was a cross-sectional study with a structured questionnaire and data were collected before using the web-based Health Assessment e-Book and 1 week after finishing. The participants were 80 nursing students who were taking the Health Assessment class from March to June 2009. RESULTS: Mean score for subjective learning achievement was 31.26 and for objective learning achievement, 69.25. Subjective and objective learning achievement were positively correlated with self-directed learning readiness, mastery goal, attitude toward distance education, and learning satisfaction. In subjective learning achievement, learning satisfaction and mastery goal were significant predictive factors and explained 64% of the variance. Objective learning achievement was significantly predicted by learning satisfaction and self-directed learning readiness, which explained 24% of the variance. CONCLUSION: Learning satisfaction, mastery goal and self-directed learning readiness were found to be very important factors associated with learning achievement for nursing students using a web-based Health Assessment e-Book. To provide high quality and effective web-based courses and to improve nursing students' learning achievement and learning satisfaction, educators should consider the learner's characteristics from the initial stages of lecture planning.
Adult ; *Computer-Assisted Instruction ; Cross-Sectional Studies ; *Education, Nursing, Baccalaureate ; Educational Status ; Female ; Humans ; Internet ; *Learning ; Male ; Personal Satisfaction ; Questionnaires ; Students, Nursing/*psychology

OBJECTIVE: To evaluate the current status and new trends in gynelcological pelviscopy in Korea. METHODS: A survey was conducted and analyzed based on 20997 cases of operative pelviscopy hom May, 1985 to February, 1998 reported from twenty four university hospitals in Korea. RESULTS: The results obtained were ss followings; 1) 45.9 % of the total cases was 30 to 39 years old age group and it was the most dominant age group, 2) The previous operative history was found in 10.9% of the tota1 cases and included cesarean section(30.0%), ectopic pregnancy(20.3%), etc. 3) The operative indications were diagnostic(26.2%), myoma uteri(23.1%), ectopic pregnancy(21.4%), adnexal mass(18.4%), etc. In cases of diagnostic indication, the most common post-operative diagnosis of pelviscopic surgery was tubal pregnancy(30,6%). 4) The operative titles were salpingectomy(25.1%), hysterectomy(19.9%), salpinghorectomy(19.8%), etc. 5) The complications of operative pelviscopy were composed of subcutaneous emphysema(0.58%), preperitoneal bleeding(0.3%), bladder injury(0,3%), bowel injury(0.3%), etc. 6) lhe survey showed that 57.1% of the respondents desired more than 1 year of period for well peration between the operator and the assistant. 56.7% of respondents indicated that the surgical technique of the operator was the most important factor for a successful pelvisocopic surgery. 7) The most beneficial effect of the pelviscopy included the comfortableness of the patient(40.0%), cosmetic effect(22.8%), decrease of complications(14.2%), etc. CONCLUSION: The modem methods of operative pelviscopy have been successfully used in the treatment of gynecological disease. Today, pelviscopic surgery is being applied in almost all the cases of gynecological disease. Hence, the majority of pelviscopy techniques and pracedures being performed by surgeon must be taught in the controlled setting of a residency or a fellowship training program.
Adult ; Surveys and Questionnaires ; Diagnosis ; Education ; Fellowships and Scholarships ; Hospitals, University ; Humans ; Internship and Residency ; Korea* ; Modems ; Myoma ; Urinary Bladder