1.Observation of 17 Asphyxial Suicides by Helium Gas.
Hyoung Soo LIM ; Kyung Won HAHM ; Hyun Wook KANG
Korean Journal of Legal Medicine 2013;37(2):78-83
Since the book "Final Exit: The Practicalities of Self-Deliverance and Assisted Suicide for the Dying" was published in 1991, there has been a worldwide increase in the number of cases of suicidal asphyxiation using helium gas. However, no domestic reports have been published thus far. Recently, we encountered a case of asphyxial suicide by inhalation of helium from inside a plastic bag. Subsequently, we reviewed the records of the Scientific Crime Analysis System of National Police Agency of Korea. There were 17 cases of helium-associated asphyxial suicides between January 1, 2000 and June 30, 2012. The average age of the deceased was 30.6 years with a male to female ratio of approximately 5:1. Thirteen of 17 such deaths occurred in the victims'houses. In all cases, the method of death involved placing a plastic bag over the head with a hose attached to helium tanks. In 6 of 17 cases, the decedents had psychiatric disorders such as depression. As neither characteristic signs of death nor conventional methods for detecting helium gas exist, a thorough investigation of the death scene and the decedents'environment is extremely vital for confirming death due to helium asphyxiation.
Crime
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Depression
;
Female
;
Head
;
Helium
;
Humans
;
Inhalation
;
Korea
;
Male
;
Plastics
;
Police
;
Suicide
;
Suicide, Assisted
2.A clinical study of degenerative spinal stenosis.
Seung Rim PARK ; Myung Ho KIM ; Hyoung Soo KIM ; Kyoung Ho MOON ; Seog Won LIM
The Journal of the Korean Orthopaedic Association 1991;26(4):1213-1218
No abstract available.
Spinal Stenosis*
3.Posterior Epidural Migration of a Sequestrated Intervertebral Lumbar Disc: A Case Report.
Jae Lim CHO ; Ye Soo PARK ; Joo Hee HAN ; Tae Hyoung KWEON
The Journal of the Korean Orthopaedic Association 1999;34(5):985-988
Posterior epidural migration of a sequestrated intervertebral lumbar disc is rare with only five cases ever reported in the literature so far. The objective of this paper is to report our experience of such a extremely rare case with a review the literature
4.Poland's syndrome: one case report.
Seung Rim PARK ; Myung Ho KIM ; Hyoung Soo KIM ; Kyoung Ho MOON ; Seog Won LIM
The Journal of the Korean Orthopaedic Association 1991;26(5):1606-1608
No abstract available.
5.A Clinical Study of Fractures and Dislocations of the Spine
Kwang Hoe KIM ; Sung Joon KIM ; Jae Lim CHO ; Hyoung Soo KIM
The Journal of the Korean Orthopaedic Association 1983;18(5):851-860
No abstract available in English.
Clinical Study
;
Dislocations
;
Spine
6.Reliability of the Single Cell PCR analysis for Preimplantation Genetic Diagnosis of Single Gene Disorders.
Hye Won CHOI ; Hyoung Song LEE ; Chun Kyu LIM ; Mi Kyoung KOONG ; Inn Soo KANG ; Jin Hyun JUN
Korean Journal of Fertility and Sterility 2005;32(4):293-300
No abstract available.
Epidermolysis Bullosa
;
Muscular Dystrophy, Duchenne
;
Ornithine Carbamoyltransferase
;
Polymerase Chain Reaction*
;
Preimplantation Diagnosis*
7.The Effects of Sciatic Neurectomy on Rat Gastrocnemius Muscle.
Sang Soo OH ; Jung Min LIM ; Hyoung Tae KIM
Korean Journal of Physical Anthropology 2008;21(2):149-165
The effects of unilateral sciatic neurectomy on gastrocnemius muscle were studied in adult male rats. The morphological changes of both gastrocnemius muscles were observed by light and electron microscopy. Western blot analysis was performed to study the protein expression. Following the denervation, the affected muscle weights decreased significantly than normal. And the denervation led to a significant reduction in the area and diameter of muscle fibers on light microscopy. The affected muscle fibers showed the decreasing in size and the irregularity of myofibrilar arrangement on electron microscopy. On transverse view, the area of affected muscle fibers were smaller than normal. Their myofibrils were smaller and very irregular in size. The thin and thick myofilaments were not regular and partially lost. Mitochondria between myofibrils and subsarcolemmal area in affected muscle fibers were damaged and partially lost. The sacoplasmic reticulum and T-tubules were mostly lost and irregular. Some satellite cells were observed adjacent the muscle fiber, but they were inactive morphologically. On longitudinal view, most of myofibrils in affected muscle fibers were lost generally or partially although the their most sarcomeres were regularly arranged. Z line and myofilaments were lost partially and were partially irregularly arranged. The loss of thin myofilaments was more than that of thick myofilaments. Much debris of cell organelles were scattered among myofibrils. The expression of MyoD and myogenin were decrease significantly and the expression of p-mTOR and p-FOXO1 were decreased, too. On the other hand, MuRF1 was increased significantly. Taken together, the main effect of gastrocnemius muscle by sciatic neurectomy is the atrophy as a result of the loss of myofilaments and cell organelles through the decrease of protein synthesis and the increase of protein degradation.
Adult
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Animals
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Atrophy
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Blotting, Western
;
Denervation
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Hand
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Humans
;
Light
;
Male
;
Microscopy
;
Microscopy, Electron
;
Mitochondria
;
Muscle, Skeletal
;
Muscles
;
Myofibrils
;
Myogenin
;
Organelles
;
Proteolysis
;
Rats
;
Reticulum
;
Sarcomeres
;
Sciatic Nerve
;
Weights and Measures
8.Delayed Local Infection in Tibial Fracture Treated with Plate Fixation.
Kee Haeng LEE ; Youn Soo KIM ; Moon Gu CHOI ; Chang Hoon JEONG ; Hyoung Min KIM ; Ki Hyeon LIM
The Journal of the Korean Orthopaedic Association 1998;33(1):127-132
Plate fixation is being regarded as one of the reliahle fixation method in the management of tibial fractures. But this technique may leave many complications including infection. However, delayed local infection which developed several weeks after open reduction on the tibia has been rarely described as a complication in the literatures. The purpose of this study is to evaluate the contributing factors to the delayed locaI infection in tibial fracture treated with plate fixation. Authors reviewed and analyzed the 60 cases of tihial fracture that were treated with plate and screws from 1989 to 1996. Analyses dealt with fracture classification, laboratory and radiographic findings and the site of plate application. We defined a delayed local infection as a condition in which patient has infection signs on Jaboratory and physical examination at or over 8 week post-operation without any evidence of osteomyelitis on plain radiographs. We found 7 cases of delayed local infection out of 60 tibial fractures from 8 to 21 weeks postoperatively with an average of 14.1 weeks. All the 7 cases that had been treated with plate on medial aspect of tibia were treated by administration of antibiotics without surgery such as debridement or removal of hardwares. Authors concluded that the medial plating was the most important factor of the development of delayed local infection(Z=1.78).
Anti-Bacterial Agents
;
Classification
;
Debridement
;
Humans
;
Osteomyelitis
;
Physical Examination
;
Tibia
;
Tibial Fractures*
9.Arachnoid Cyst in Cavernous Sinus: Case Report.
Hyoung Gun LIM ; Won Jong YOO ; So Lyung JUNG ; Hae Giu LEE ; Hyun Wook LIM ; Soo Ah IM
Journal of the Korean Radiological Society 2002;47(6):561-563
Arachnoid cyst of the cavernous sinus is very rare. When present, its anatomic location frequently gives rise to cranial nerve palsy. We report a case of arachnoid cyst of the cavernous sinus in a 38-year-old man with impaired eyeball movement and diplopia.
Adult
;
Arachnoid Cysts
;
Arachnoid*
;
Cavernous Sinus*
;
Cranial Nerve Diseases
;
Diplopia
;
Humans
10.The Electron Microscopic Study on the Development of Knee Joint in Rat.
Hyoung Soo LIM ; Won Hwan OH ; Jae Young LIM ; Yong Wook KIM ; Wang Jae LEE ; Ka Young CHANG ; Douk Ho HWANG
Korean Journal of Anatomy 2000;33(3):339-351
These study was designed to observe the appearance and the characteristics of apoptotic cells during the development of knee joint in rat. The fetus were collected on the 16th, 17th, 18th, 19th, and 20th day of pregnancy. In this study, TUNEL staining, electron microscopic investigation and immunocytochemical gold labeling techniques were used. In the immuno-cytochemical gold labeling techniques, primary antibodies were used, which were to be polyclonal rabbit anti-mouse/ rat Bax, polyclonal rabbit anti-tissue transglutaminase C, and polyclonal goat anti-cpp32p20. The samples were observed under JEOL 1200 EX-II transmission electron microscope. The results were as follows. 1. In a 16-day-old fetus, between femur and tibia cartilages, mesenchymal cells were observed. Mesenchymal cells had marginated heterochromatin and dilated rough endoplasmic reticulum. 2. In a 17-day-old fetus, the knee joint clefts were first formed. In the primordial cruciate ligaments between the cartilages, capillaries were scattered. The apoptotic cells, which had fragmented and condensed nucleus, showed in the synovium. And necrotic cells, which had nuclear chromatin margination, perinuclear cisternae, and dilated rough endoplasmic reticulum, also were observed in the joint cleft surface. 3. From the 18-day-old fetus, phagocytic synovial cells and secretory synovial cells could be confirmed. The apoptotic cells were not seen. 4. In a 17-day-old fetus, a few cells were positive for TUNEL reaction in the joint cleft region. 5. In a 17-day-old fetus, Bax were marked on the mitochondria, endoplasmic reticulum of apoptotic cells. Also, it was marked at the phagocytosed apoptotic bodies in the neighboring cells. 6. In a 17-day-old fetus, the tissue Transglutaminase C were marked in the perinuclear region, vacuoles, cell membrane and extracellular matrix of the apoptotic cells. Also, it was marked at the phagocytosed apoptotic bodies in the neighboring cells. 7. In a 17-days-old fetus, CPP32 labeling were marked in the cytoplasm of the apoptotic cells. Practically, it was distributed between the phagocytosed apoptotic bodies and the neighboring cells. On the basis of above findings, it is obvious that the joint cleft are first formed in a 17-day-old fetus, a few cells are to be TUNEL positive signals, and the apoptotic cells contain Bax, tissue Transglutaminase C, and CPP32. Therefore the apoptotic cells and the necrotic cells are appeared in the 17-day-old fetus, and these cells are concerned with joint cleft formation.
Animals
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Antibodies
;
Apoptosis
;
Capillaries
;
Cartilage
;
Cell Membrane
;
Chromatin
;
Cytoplasm
;
Endoplasmic Reticulum
;
Endoplasmic Reticulum, Rough
;
Extracellular Matrix
;
Femur
;
Fetus
;
Goats
;
Heterochromatin
;
In Situ Nick-End Labeling
;
Joints
;
Knee Joint*
;
Knee*
;
Ligaments
;
Mitochondria
;
Pregnancy
;
Rats*
;
Synovial Membrane
;
Tibia
;
Vacuoles