2.Generation and Characterization of Alloenic Radiation Bone Marrow Chimera.
Korean Journal of Immunology 1998;20(3):333-341
Allogenic bone marrow chimera has been used to study the differentiation of donor-derived bone marrow cells under the recipient thymic environment, tolerance generaion between recipient and donor cells. We prepared H-2' to H-2 allogenic chimera by transfering bone marrow cells from H-2dmice to r-irradiated H-2k mice, and examined the differentiation ofthe bone marrow cells under allogenic environment. Complete reconstitution with H-2d+ phenotype cells in the thymus of the chimera mice was observed. However, the number of CD4- CD8+ cells dramatically decreased under the recipient thymic environment, CD8+ cells significantly reduced also in spleen and lymph node, compared with that of normal mice. Interestingly, we also observed coexistence of donor-derived cells (H-2k) and recipient derived cells (H-2d) in lymph node and spleen in the chimera. These results suggested that the decrease of CD4-CD8+ cells could be caused by r-irradiation by affecting the recipient thymic environment, and that in this chimera, tolerance between donor-derived cells and recipient-derived cells was maintained.
Animals
;
Bone Marrow Cells
;
Bone Marrow*
;
Chimera*
;
Humans
;
Lymph Nodes
;
Mice
;
Phenotype
;
Spleen
;
Thymus Gland
;
Tissue Donors
3.Clinicopathologic parameters in predicting cervical nodal metastasis in early squamous cell carcinoma of the oral cavity.
Hyoun Chull KIM ; Jingo KUSUKAWA ; Tadamitsu KAMEYAMA
Journal of the Korean Association of Oral and Maxillofacial Surgeons 1992;18(3):90-97
No abstract available.
Carcinoma, Squamous Cell*
;
Mouth*
;
Neoplasm Metastasis*
4.A Study of Culture and Sensitivity Test of Mycobacterium Tuberculosis
The Journal of the Korean Orthopaedic Association 1977;12(4):681-684
A study of concentration smear, culture for AFB and sensitivity test for antituberculous drugs was carried out in 810 patients who had been admitted in SNUH in the period of 12 months from January 1976 to December 1976. Acid-fast bacilli was confirmed in 110 cases (13.6%) by concentrated direct smear and in 88 cases(10.9%) by culture, and concomitantly sensitivity test was performed in 26 isolated cases. Streptomycin, INH, PAS, Kanamycin and Ethionamide were rather less sensitive to the strains of Mycobacterium tuberculosis, but all strains obtained during the period of this study were highly sensitive to Rifampicin.
Ethionamide
;
Humans
;
Kanamycin
;
Mycobacterium tuberculosis
;
Mycobacterium
;
Rifampin
;
Streptomycin
5.T Cell Epitope Analysis of Structural Protein of Adenovirus.
Jae Won HWANG ; Mi Hyung KIM ; Kil Hyoun KIM
Korean Journal of Immunology 1998;20(4):435-442
Thelper (Th) cells play a pivotal role in the regulation of immune responses. Since Th epitopes in adenovirus have not yet been defined, in this study, it was attempted to search for Th epitopes of adenovirus antigens that are restricted by MHC class II (H-2E). Among candidate viral proteins to be screened for Th epitopes, structural protein was selected, since they induced strong IL-2 release from adenovirus immune lyrnph node (LN) cells and the presence of E1 protein, which contains immunodominant cytotoxic T lymphocyte epitopes, did not potentiate the T cell responses. To confirm the presence of Th epitopes in the structural protein, virions were trypsinized and the resulting polypeptides whose molecular weights were lower than 5,000 were fractionated by HPLC. Some of the HPLC fraction turned out to induce LN cell proliferation. Ten synthetic peptides were designed as candidate Th epitopes from the primary amino acid sequences of adenovirus hexon and penton protein which are major constituents of the virion. The selected sequences share the common features of other known H-2E' binding ligands. Among these ten synthetic peptides, peptide of hexon protein amino acid residue 709-721 induced noticeable proliferation of LN cells from preimmune mice, and also able to induce IL-2 secretion from adenovirus-specific T hybridomas, suggesting that the peptide was the most immunodominant Th epitope. Hexon protein 221-233 and hexon protein 676-688 are considered as epitopes also. This study revealed three epitope sequences from adenovirus structural protein that are presented by class II MHC, H-2E.
Adenoviridae*
;
Amino Acid Sequence
;
Animals
;
Cell Proliferation
;
Chromatography, High Pressure Liquid
;
Epitopes
;
Epitopes, T-Lymphocyte*
;
Hybridomas
;
Interleukin-2
;
Ligands
;
Mice
;
Molecular Weight
;
Peptides
;
Trypsin
;
Viral Proteins
;
Virion
6.The in Vitro Proliferative Properties of Fibroblasts Originating from Upper and Lower Dermis of Psoriatic Skin Lesions.
Jong Min KIM ; Hyoun Chan CHO ; Yoo Shin LEE
Korean Journal of Dermatology 1987;25(1):41-51
To investigate the heterogeneity of fibroblast proliferation rate depending on the depth of dermis in the psoriatic skin lesions, fibroblasts were obtained from the upper and lower dermis of the forearm skin lesions in 9 psoriatir, patients and of the comparable sites in 7 healthy persons respectively, and were cultured. The in vitro proliferation rates of the fibroblasts were calculated by measuring the efficiency af cell attachment, cell numbers on varying days of culture and the population doubling time during the 3rd passage of subculture. The results were as follows: 1, The efficiencies of cell attachment at 24 hours after seeding were not statistically different between the upper derrnal fibroblasts and the lower ones in both psoriatic skin lesions and normal skins. 2. By rneasuring the cell numbers and the population doubling times, the prolifeatior rate of the upper der mal fibroblasts was greater than that of the lower ones in both psoriatic skin lesions and normal skins and that the fibroblastic proliferation rate was significantly inereased in the psoriatic skin lesion compared to the normal skin.
Cell Count
;
Dermis*
;
Fibroblasts*
;
Forearm
;
Humans
;
Population Characteristics
;
Skin*
7.Influence of Gamma linoleic acid (Epogam) on the Skin Surface Conditions of Atopic Dermatitis.
Hyoun Seung LEE ; Kyoung Chan PARK ; Kyu Han KIM
Annals of Dermatology 2000;12(4):238-242
BACKGROUND: Gamma linoleic acid (GLA, Epogam) is considered a safe and effective modality in the treatment of atopic dermatitis (AD) in which impaired function of the enzyme, delta-6-desaturase, has been reported to result in reduced levels of GLA, desaturated fatty acids. OBJECTIVE: We performed this study to observe the changes of skin surface conditions measured objectively by bioengineering methods in relation to clinical improvement after treatment with GLA (Epogam®) in children with AD. METHODS: Thirty-four children with AD were treated with GLA (Epogam®) and evaluated with clinical parameters.The changes of skin surface conditions were monitored by non-invasive experimental instruments. RESULTS: There was a significant decrease of transepidermal water loss (TEWL) and gradual improvements in clinical severity after 12 weeks of GLA (Epogam®) treatment. The change of skin surface pH was statistically significant on the antecubital fossa and abdomen except the popliteal fossa. The other parameters including skin surface hydration and skin surface lipid did not show consistent changes. CONCLUSION: Clinical improvement of AD with GLA (Epogam) seemed to be achieved by the reduction of TEWL.
Abdomen
;
Bioengineering
;
Child
;
Dermatitis, Atopic*
;
Fatty Acids
;
Humans
;
Hydrogen-Ion Concentration
;
Linoleic Acid*
;
Linoleoyl-CoA Desaturase
;
Skin*
;
Water
8.Evaluation of ACL-300 automated coagulation analyzer for the plasmafibrinogen assay.
Hyoun Tae KIM ; Ae Ja PARK ; Young Ju CHA
Korean Journal of Clinical Pathology 1992;12(2):195-204
No abstract available.
9.Evaluation of ACL-300 automated coagulation analyzer for the plasmafibrinogen assay.
Hyoun Tae KIM ; Ae Ja PARK ; Young Ju CHA
Korean Journal of Clinical Pathology 1992;12(2):195-204
No abstract available.
10.Evaluation for counting reticulocytes by FACScan.
Ae Ja PARK ; Hyoun Tae KIM ; Yong Ook PARK
Korean Journal of Clinical Pathology 1993;13(2):219-223
No abstract available.
Reticulocytes*