1.Isolation of Middle East Respiratory Syndrome Coronavirus from a Patient of the 2015 Korean Outbreak.
Wan Beom PARK ; Nak Jung KWON ; Pyoeng Gyun CHOE ; Su Jin CHOI ; Hong Sang OH ; Sang Min LEE ; Hyonyong CHONG ; Jong Il KIM ; Kyoung Ho SONG ; Ji Hwan BANG ; Eu Suk KIM ; Hong Bin KIM ; Sang Won PARK ; Nam Joong KIM ; Myoung Don OH
Journal of Korean Medical Science 2016;31(2):315-320
During the 2015 outbreak of Middle East respiratory syndrome coronavirus (MERS-CoV) in Korea, 186 persons were infected, resulting in 38 fatalities. We isolated MERS-CoV from the oropharyngeal sample obtained from a patient of the outbreak. Cytopathic effects showing detachment and rounding of cells were observed in Vero cell cultures 3 days after inoculation of the sample. Spherical virus particles were observed by transmission electron microscopy. Full-length genome sequence of the virus isolate was obtained and phylogenetic analyses showed that it clustered with clade B of MERS-CoV.
Animals
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Cercopithecus aethiops
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Coronavirus Infections/*diagnosis/epidemiology/*virology
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Disease Outbreaks
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Humans
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Microscopy, Electron
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Middle East Respiratory Syndrome Coronavirus/classification/genetics/*isolation & purification/ultrastructure
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Phylogeny
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Polymerase Chain Reaction
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RNA, Viral/analysis/chemistry/metabolism
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Republic of Korea/epidemiology
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Sequence Analysis, RNA
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Vero Cells
Result Analysis
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