1.Rapid Sequential Gain of ABL1 Kinase Domain Mutations with a Complex Karyotype in the Progression of Chronic Myelogenous Leukemia.
Yousun CHUNG ; Hyeon Seok EOM ; Hyewon LEE ; Sunseob PARK ; Hyoeun SHIM ; Eun Hae CHO ; Sun Young KONG
Annals of Laboratory Medicine 2014;34(5):399-401
No abstract available.
Base Sequence
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Benzamides/therapeutic use
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Bone Marrow/pathology
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Female
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Fusion Proteins, bcr-abl/*genetics
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Humans
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Karyotyping
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive/drug therapy/*genetics
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Middle Aged
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Philadelphia Chromosome
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Piperazines/therapeutic use
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Protein Kinase Inhibitors/therapeutic use
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Pyrimidines/therapeutic use
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Sequence Analysis, DNA
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Thiazoles/therapeutic use
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Translocation, Genetic
2.A Case of Catheter-Related Bacteremia of Tsukamurella pulmonis.
Hyoeun Eun SHIM ; Heungsup SUNG ; Seung Mi BAEK ; Seung NAMGUNG ; Mi Na KIM ; Yong Gyun KIM ; Gyu Hyung LEE
The Korean Journal of Laboratory Medicine 2009;29(1):41-47
Tsukamurella pulmonis is an aerobic actinomycete. We report a catheter-related bacteremia of T. pulmonis. A 39 yr-old male with ALL was hospitalized to receive bone marrow transplantation (BMT). Although the patient developed a high fever at the 7th hospital day (HD), it subsided with vancomycin treatment, and he received BMT at 9th HD. Fever resurged at 16th HD despite sustained treatment with vancomycin, meropenem, and amphotericin B, but subsided with removal of Hickman catheter (HC) at 19th HD. Three sets of blood cultures comprising one from the HC and two from venipunctures were taken at 7th, 16th, and 19th HD, and the distal tip of the HC was also cultured. The aerobic vials of all 3 HC-withdrawn blood cultures and one peripheral blood culture taken at 19HD and the HC tip culture grew long, straight, thin gram-positive rods that were positive on modified Kinyoun stain. This organism showed tiny, rough, grey colonies after 3-day incubation and grew to large flat colonies when incubation was extended. It was catalase-positive, urease-positive, and alkaline-slant/alkaline-deep on triple sugar iron agar, and hydrolyzed hypoxanthine. The sequence of 1,296 base pairs of 16S rRNA of this organism showed a 100.0% homology with the published sequence of T. pulmonis DSM 44142T. To our knowledge, this is the first report of T. pulmonis bacteremia in Korea.
Actinomycetales/classification/genetics/isolation & purification
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Actinomycetales Infections/diagnosis/*microbiology/therapy
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Adult
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Bacteremia/*diagnosis/microbiology/therapy
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Bone Marrow Transplantation
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Catheter-Related Infections/*microbiology
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Humans
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Leukemia, Myeloid, Acute/therapy
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Male
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Phylogeny
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RNA, Ribosomal, 16S/genetics
3.Development of an Automated Image Analyzer for Microvessel Density Measurement in Bone Marrow Biopsies
Yousun CHUNG ; Seungwon SHIN ; Hyoeun SHIM ; Ji Yeon SOHN ; Dong eun LEE ; Hyewon LEE ; Hyeon Seok EOM ; Kwang Gi KIM ; Sun Young KONG
Annals of Laboratory Medicine 2020;40(4):312-316
Angiogenesis is important for the proliferation and survival of multiple myeloma (MM) cells. Bone marrow (BM) microvessel density (MVD) is a useful marker of angiogenesis and an increase in MVD can be used as a marker of poor prognosis in MM patients. We developed an automated image analyzer to assess MVD from images of BM biopsies stained with anti-CD34 antibodies using two color models. MVD was calculated by merging images from the red and hue channels after eliminating non-microvessels. The analyzer results were compared with those obtained by two experienced hematopathologists in a blinded manner using the 84 BM samples of MM patients. Manual assessment of the MVD by two hematopathologists yielded mean±SD values of 19.4±11.8 and 20.0±11.8. The analyzer generated a mean±SD of 19.5±11.2. The intraclass correlation coefficient (ICC) and Bland-Altman plot of the MVD results demonstrated very good agreement between the automated image analyzer and both hematopathologists (ICC=0.893 [0.840–0.929] and ICC=0.906 [0.859–0.938]). This automated analyzer can provide time- and labor-saving benefits with more objective results in hematology laboratories.
4.Prevalence and Risk Factors of Germline Pathogenic Variants in Pancreatic Ductal Adenocarcinoma
Kum Hei RYU ; Sunhwa PARK ; Jung Won CHUN ; Eunhae CHO ; Jongmun CHOI ; Dong-Eun LEE ; Hyoeun SHIM ; Yun-Hee KIM ; Sung-Sik HAN ; Sang-Jae PARK ; Sang Myung WOO ; Sun-Young KONG
Cancer Research and Treatment 2023;55(4):1303-1312
Purpose:
The genetic attribution for pancreatic ductal adenocarcinoma (PDAC) has been reported as 5%-10%. However, the incidence of germline pathogenic variants (PVs) in Korean PDAC patients has not been thoroughly investigated. Therefore, we studied to identify the risk factors and prevalence of PV for future treatment strategies in PDAC.
Materials and Methods:
Total of 300 (155 male) patients with a median age of 65 years (range, 33 to 90 years) were enrolled in National Cancer Center in Korea. Cancer predisposition genes, clinicopathologic characteristics, and family history of cancer were analyzed.
Results:
PVs were detected in 20 patients (6.7%, median age 65) in ATM (n=7, 31.8%), BRCA1 (n=3, 13.6%), BRCA2 (n=3), and RAD51D (n=3). Each one patient showed TP53, PALB2, PMS2, RAD50, MSH3, and SPINK1 PV. Among them, two likely PVs were in ATM and RAD51D, respectively. Family history of various types of cancer including pancreatic cancer (n=4) were found in 12 patients. Three patients with ATM PVs and a patient with three germline PVs (BRCA2, MSH3, and RAD51D) had first-degree relatives with pancreatic cancer. Familial pancreatic cancer history and PVs detection had a significant association (4/20, 20% vs. 16/264, 5.7%; p=0.035).
Conclusion
Our study demonstrated that germline PVs in ATM, BRCA1, BRCA2, and RAD51D are most frequent in Korean PDAC patients and it is comparable to those of different ethnic groups. Although this study did not show guidelines for germline predisposition gene testing in patients with PDAC in Korea, it would be emphasized the need for germline testing for all PDAC patients.